MyulA.00612.a
Lysine--tRNA ligase LysS (EC 6.1.1.6) (Lysyl-tRNA synthetase)
| CENTER ID: | MyulA.00612.a |
| ORGANISM: | Mycobacterium ulcerans Agy99 |
| ASSOCIATED DISEASE: | Buruli, Bairnsdale ulcer |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyulA.00612.a.B1.GE40601 | full length | 1 | 498 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyulA.00612.a.B1.PW37993 | full length | 1 | 498 |
Structures
5VL1
DEPOSITED: 4/24/2017
DETERMINATION: XRay
CLONE: MyulA.00612.a.B1.GE40601
PROTEIN: MyulA.00612.a.B1.PW37993
6AQG
DEPOSITED: 8/19/2017
DETERMINATION: XRay
CLONE: MyulA.00612.a.B1.GE40601
PROTEIN: MyulA.00612.a.B1.PW37993
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|362242.7.peg.4857 |
| UniProt: | A0PV47 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSAESNIPE QFRIRRDKRA RLLAEGYDPY PVAIERTHTL AEIRATYADL PTDSATEDIV GVAGRVVFAR NTGKLCFATL QDGDGTQLQA MISLDEVGRE SLDRWKADVD IGDVVYVHGT VISSRRGELS VLADSWRMAA KALRPLPVAH KEMSEESRVR QRYVDLIVRP QAREVARQRI AVIRAVRNAL ERRGFLEVET PMLQTLAGGA AARPFVTHSN ALDIDLYLRI APELFLKRCI VGGFDRVFEL NRVFRNEGSD STHSPEFSML ETYQTYGTYD DSALITRELI QEVADEAIGT RQLSMPDGSV YDIDGEWATM EMYSSLSEAL GEQITPETTV ARLRDIASGL DVEIDNSVFG HGKLVEELWE HAVGNKLTAP TFVKDFPVET TPLTRQHRSI PGVTEKWDLY VRGVELATGY SELNDPVVQR DRFADQARAA AAGDDEAMQL DEDFLTALEY GMPPCTGTGM GIDRLLMCLT GLSIRETVLF PIVRPHSN
NT Sequence
gtgagctccg ccgagtcaaa cattcccgag cagttccgaa tccgtcggga caagcgtgct cggttattgg cagaggggta tgacccctat ccggtagcca tcgaacgcac tcacacgttg gccgagattc gtgccaccta tgccgaccta ccgacagatt cggcgaccga ggacatcgtc ggtgtcgcgg gtcgagtggt cttcgcgcgt aacaccggga aactgtgttt tgcgacactg caggacggcg acggcaccca gctgcaggcg atgatcagcc tcgacgaggt gggccgcgaa tcactggaca ggtggaaggc cgacgtcgac atcggtgacg tcgtctacgt gcacggcacc gtgatcagct cccgtcgggg cgaactatcg gtgctcgccg attcctggcg gatggctgcc aaggccttgc ggccgctgcc ggtcgcacac aaggagatga gcgaggagtc gcgggttcgt cagcgctacg tcgatttgat cgtgcgtcca caggcgcggg aggtcgcgcg acagcggatt gcggtaatcc gtgcggtgcg taatgcgctg gagcggcgcg ggtttctcga agtcgaaacg ccaatgttgc agaccttggc cggcggcgct gcggcgcggc cgttcgtcac ccattccaat gccctcgaca tcgatcttta cctgcgtatc gcgccggaac tgttcctcaa gcgttgcatc gtgggcggtt tcgacagggt ctttgaacta aatcgggtgt tccgaaacga agggtctgat tccacccatt ccccggaatt ctccatgctg gagacgtatc agacctatgg aacctatgac gattcggccc tgatcaccag agagcttatt caagaagttg ctgacgaggc gatcggaacc agacaactct cgatgcctga cggcagcgtg tacgacatcg acggagaatg ggcaactatg gagatgtatt cctccttgtc ggaggcactg ggggaacaga tcacgccaga gacgacggtt gctcgcttac gtgacatcgc aagcggactt gacgtagaga ttgacaacag tgtttttggt cacggcaaac ttgtcgagga actctgggaa catgctgtcg gcaacaagtt gacggcgccc acatttgtca aggatttccc agtcgagaca acgccattga ctcgtcagca ccgcagtatc cccggagtaa ccgagaagtg ggatctctac gtgcgcggag tcgaactggc taccgggtac tcggaattaa acgatcccgt cgtccagcgg gacagattcg ctgatcaggc ccgtgcggcg gccgcgggtg acgacgaggc gatgcaactt gacgaagatt ttctgacggc tctggagtac gggatgcccc cgtgtactgg aactggaatg ggcatcgatc ggttgttgat gtgtttgacc ggactgtcaa ttagggagac agttttgttc ccgattgttc gccctcactc caactga
Details for MyulA.00612.a.B1.GE40601
| HARVESTED ON: | 10/18/2016 |
| SEQUENCED ON: | 10/19/2016 |
| EXPECTED MW: | 56kDa |
| OBSERVED MW: | 58kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMV SSAESNIPEQ FRIRRDKRAR LLAEGYDPYP VAIERTHTLA EIRATYADLP TDSATEDIVG VAGRVVFARN TGKLCFATLQ DGDGTQLQAM ISLDEVGRES LDRWKADVDI GDVVYVHGTV ISSRRGELSV LADSWRMAAK ALRPLPVAHK EMSEESRVRQ RYVDLIVRPQ AREVARQRIA VIRAVRNALE RRGFLEVETP MLQTLAGGAA ARPFVTHSNA LDIDLYLRIA PELFLKRCIV GGFDRVFELN RVFRNEGSDS THSPEFSMLE TYQTYGTYDD SALITRELIQ EVADEAIGTR QLSMPDGSVY DIDGEWATME MYSSLSEALG EQITPETTVA RLRDIASGLD VEIDNSVFGH GKLVEELWEH AVGNKLTAPT FVKDFPVETT PLTRQHRSIP GVTEKWDLYV RGVELATGYS ELNDPVVQRD RFADQARAAA AGDDEAMQLD EDFLTALEYG MPPCTGTGMG IDRLLMCLTG LSIRETVLF
Validated NT Sequence
cgggaacaaa actgtctccc taattgacag tccggtcaaa cacatcaaca accgatcgat gcccattcca gttccagtac acgggggcat cccgtactcc agagccgtca gaaaatcttc gtcaagttgc atcgcctcgt cgtcacccgc ggccgccgca cgggcctgat cagcgaatct gtcccgctgg acgacgggat cgtttaattc cgagtacccg gtagccagtt cgactccgcg cacgtagaga tcccacttct cggttactcc ggggatactg cggtgctgac gagtcaatgg cgttgtctcg actgggaaat ccttgacaaa tgtgggcgcc gtcaacttgt tgccgacagc atgttcccag agttcctcga caagtttgcc gtgaccaaaa acactgttgt caatctctac gtcaagtccg cttgcgatgt cacgtaagcg agcaaccgtc gtctctggcg tgatctgttc ccccagtgcc tccgacaagg aggaatacat ctccatagtt gcccattctc cgtcgatgtc gtacacgctg ccgtcaggca tcgagagttg tctggttccg atcgcctcgt cagcaacttc ttgaataagc tctctggtga tcagggccga atcgtcatag gttccatagg tctgatacgt ctccagcatg gagaattccg gggaatgggt ggaatcagac ccttcgtttc ggaacacccg atttagttca aagaccctgt cgaaaccgcc cacgatgcaa cgcttgagga acagttccgg cgcgatacgc aggtaaagat cgatgtcgag ggcattggaa tgggtgacga acggccgcgc cgcagcgccg ccggccaagg tctgcaacat tggcgtttcg acttcgagaa acccgcgccg ctccagcgca ttacgcaccg cacggattac cgcaatccgc tgtcgcgcga cctcccgcgc ctgtggacgc acgatcaaat cgacgtagcg ctgacgaacc cgcgactcct cgctcatctc cttgtgtgcg accggcagcg gccgcaaggc cttggcagcc atccgccagg aatcggcgag caccgatagt tcgccccgac gggagctgat cacggtgccg tgcacgtaga cgacgtcacc gatgtcgacg tcggccttcc acctgtccag tgattcgcgg cccacctcgt cgaggctgat catcgcctgc agctgggtgc cgtcgccgtc ctgcagtgtc gcaaaacaca gtttcccggt gttacgcgcg aagaccactc gacccgcgac accgacgatg tcctcggtcg ccgaatctgt cggtaggtcg gcataggtgg cacgaatctc ggccaacgtg tgagtgcgtt cgatggctac cggatagggg tcatacccct ctgccaataa ccgagcacgc ttgtcccgac ggattcggaa ctgctcggga atgtttgact cggcggagct caccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS SAESNIPEQF RIRRDKRARL LAEGYDPYPV AIERTHTLAE IRATYADLPT DSATEDIVGV AGRVVFARNT GKLCFATLQD GDGTQLQAMI SLDEVGRESL DRWKADVDIG DVVYVHGTVI SSRRGELSVL ADSWRMAAKA LRPLPVAHKE MSEESRVRQR YVDLIVRPQA REVARQRIAV IRAVRNALER RGFLEVETPM LQTLAGGAAA RPFVTHSNAL DIDLYLRIAP ELFLKRCIVG GFDRVFELNR VFRNEGSDST HSPEFSMLET YQTYGTYDDS ALITRELIQE VADEAIGTRQ LSMPDGSVYD IDGEWATMEM YSSLSEALGE QITPETTVAR LRDIASGLDV EIDNSVFGHG KLVEELWEHA VGNKLTAPTF VKDFPVETTP LTRQHRSIPG VTEKWDLYVR GVELATGYSE LNDPVVQRDR FADQARAAAA GDDEAMQLDE DFLTALEYGM PPCTGTGMGI DRLLMCLTGL SIRETVLFPI VRPHSN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagct ccgccgagtc aaacattccc gagcagttcc gaatccgtcg ggacaagcgt gctcggttat tggcagaggg gtatgacccc tatccggtag ccatcgaacg cactcacacg ttggccgaga ttcgtgccac ctatgccgac ctaccgacag attcggcgac cgaggacatc gtcggtgtcg cgggtcgagt ggtcttcgcg cgtaacaccg ggaaactgtg ttttgcgaca ctgcaggacg gcgacggcac ccagctgcag gcgatgatca gcctcgacga ggtgggccgc gaatcactgg acaggtggaa ggccgacgtc gacatcggtg acgtcgtcta cgtgcacggc accgtgatca gctcccgtcg gggcgaacta tcggtgctcg ccgattcctg gcggatggct gccaaggcct tgcggccgct gccggtcgca cacaaggaga tgagcgagga gtcgcgggtt cgtcagcgct acgtcgattt gatcgtgcgt ccacaggcgc gggaggtcgc gcgacagcgg attgcggtaa tccgtgcggt gcgtaatgcg ctggagcggc gcgggtttct cgaagtcgaa acgccaatgt tgcagacctt ggccggcggc gctgcggcgc ggccgttcgt cacccattcc aatgccctcg acatcgatct ttacctgcgt atcgcgccgg aactgttcct caagcgttgc atcgtgggcg gtttcgacag ggtctttgaa ctaaatcggg tgttccgaaa cgaagggtct gattccaccc attccccgga attctccatg ctggagacgt atcagaccta tggaacctat gacgattcgg ccctgatcac cagagagctt attcaagaag ttgctgacga ggcgatcgga accagacaac tctcgatgcc tgacggcagc gtgtacgaca tcgacggaga atgggcaact atggagatgt attcctcctt gtcggaggca ctgggggaac agatcacgcc agagacgacg gttgctcgct tacgtgacat cgcaagcgga cttgacgtag agattgacaa cagtgttttt ggtcacggca aacttgtcga ggaactctgg gaacatgctg tcggcaacaa gttgacggcg cccacatttg tcaaggattt cccagtcgag acaacgccat tgactcgtca gcaccgcagt atccccggag taaccgagaa gtgggatctc tacgtgcgcg gagtcgaact ggctaccggg tactcggaat taaacgatcc cgtcgtccag cgggacagat tcgctgatca ggcccgtgcg gcggccgcgg gtgacgacga ggcgatgcaa cttgacgaag attttctgac ggctctggag tacgggatgc ccccgtgtac tggaactgga atgggcatcg atcggttgtt gatgtgtttg accggactgt caattaggga gacagttttg ttcccgattg ttcgccctca ctccaactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MyulA.00612.a.B1.PW37993
| PURIFICATION DATe: | 12/12/2016 |
| CONCENTRATION: | 28mg/ml |
| OBSERVED MW: | 66kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV SSAESNIPEQ FRIRRDKRAR LLAEGYDPYP VAIERTHTLA EIRATYADLP TDSATEDIVG VAGRVVFARN TGKLCFATLQ DGDGTQLQAM ISLDEVGRES LDRWKADVDI GDVVYVHGTV ISSRRGELSV LADSWRMAAK ALRPLPVAHK EMSEESRVRQ RYVDLIVRPQ AREVARQRIA VIRAVRNALE RRGFLEVETP MLQTLAGGAA ARPFVTHSNA LDIDLYLRIA PELFLKRCIV GGFDRVFELN RVFRNEGSDS THSPEFSMLE TYQTYGTYDD SALITRELIQ EVADEAIGTR QLSMPDGSVY DIDGEWATME MYSSLSEALG EQITPETTVA RLRDIASGLD VEIDNSVFGH GKLVEELWEH AVGNKLTAPT FVKDFPVETT PLTRQHRSIP GVTEKWDLYV RGVELATGYS ELNDPVVQRD RFADQARAAA AGDDEAMQLD EDFLTALEYG MPPCTGTGMG IDRLLMCLTG LSIRETVLF
Validated NT Sequence
cgggaacaaa actgtctccc taattgacag tccggtcaaa cacatcaaca accgatcgat gcccattcca gttccagtac acgggggcat cccgtactcc agagccgtca gaaaatcttc gtcaagttgc atcgcctcgt cgtcacccgc ggccgccgca cgggcctgat cagcgaatct gtcccgctgg acgacgggat cgtttaattc cgagtacccg gtagccagtt cgactccgcg cacgtagaga tcccacttct cggttactcc ggggatactg cggtgctgac gagtcaatgg cgttgtctcg actgggaaat ccttgacaaa tgtgggcgcc gtcaacttgt tgccgacagc atgttcccag agttcctcga caagtttgcc gtgaccaaaa acactgttgt caatctctac gtcaagtccg cttgcgatgt cacgtaagcg agcaaccgtc gtctctggcg tgatctgttc ccccagtgcc tccgacaagg aggaatacat ctccatagtt gcccattctc cgtcgatgtc gtacacgctg ccgtcaggca tcgagagttg tctggttccg atcgcctcgt cagcaacttc ttgaataagc tctctggtga tcagggccga atcgtcatag gttccatagg tctgatacgt ctccagcatg gagaattccg gggaatgggt ggaatcagac ccttcgtttc ggaacacccg atttagttca aagaccctgt cgaaaccgcc cacgatgcaa cgcttgagga acagttccgg cgcgatacgc aggtaaagat cgatgtcgag ggcattggaa tgggtgacga acggccgcgc cgcagcgccg ccggccaagg tctgcaacat tggcgtttcg acttcgagaa acccgcgccg ctccagcgca ttacgcaccg cacggattac cgcaatccgc tgtcgcgcga cctcccgcgc ctgtggacgc acgatcaaat cgacgtagcg ctgacgaacc cgcgactcct cgctcatctc cttgtgtgcg accggcagcg gccgcaaggc cttggcagcc atccgccagg aatcggcgag caccgatagt tcgccccgac gggagctgat cacggtgccg tgcacgtaga cgacgtcacc gatgtcgacg tcggccttcc acctgtccag tgattcgcgg cccacctcgt cgaggctgat catcgcctgc agctgggtgc cgtcgccgtc ctgcagtgtc gcaaaacaca gtttcccggt gttacgcgcg aagaccactc gacccgcgac accgacgatg tcctcggtcg ccgaatctgt cggtaggtcg gcataggtgg cacgaatctc ggccaacgtg tgagtgcgtt cgatggctac cggatagggg tcatacccct ctgccaataa ccgagcacgc ttgtcccgac ggattcggaa ctgctcggga atgtttgact cggcggagct caccatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMS SAESNIPEQF RIRRDKRARL LAEGYDPYPV AIERTHTLAE IRATYADLPT DSATEDIVGV AGRVVFARNT GKLCFATLQD GDGTQLQAMI SLDEVGRESL DRWKADVDIG DVVYVHGTVI SSRRGELSVL ADSWRMAAKA LRPLPVAHKE MSEESRVRQR YVDLIVRPQA REVARQRIAV IRAVRNALER RGFLEVETPM LQTLAGGAAA RPFVTHSNAL DIDLYLRIAP ELFLKRCIVG GFDRVFELNR VFRNEGSDST HSPEFSMLET YQTYGTYDDS ALITRELIQE VADEAIGTRQ LSMPDGSVYD IDGEWATMEM YSSLSEALGE QITPETTVAR LRDIASGLDV EIDNSVFGHG KLVEELWEHA VGNKLTAPTF VKDFPVETTP LTRQHRSIPG VTEKWDLYVR GVELATGYSE LNDPVVQRDR FADQARAAAA GDDEAMQLDE DFLTALEYGM PPCTGTGMGI DRLLMCLTGL SIRETVLFPI VRPHSN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGCT CCGCCGAGTC AAACATTCCC GAGCAGTTCC GAATCCGTCG GGACAAGCGT GCTCGGTTAT TGGCAGAGGG GTATGACCCC TATCCGGTAG CCATCGAACG CACTCACACG TTGGCCGAGA TTCGTGCCAC CTATGCCGAC CTACCGACAG ATTCGGCGAC CGAGGACATC GTCGGTGTCG CGGGTCGAGT GGTCTTCGCG CGTAACACCG GGAAACTGTG TTTTGCGACA CTGCAGGACG GCGACGGCAC CCAGCTGCAG GCGATGATCA GCCTCGACGA GGTGGGCCGC GAATCACTGG ACAGGTGGAA GGCCGACGTC GACATCGGTG ACGTCGTCTA CGTGCACGGC ACCGTGATCA GCTCCCGTCG GGGCGAACTA TCGGTGCTCG CCGATTCCTG GCGGATGGCT GCCAAGGCCT TGCGGCCGCT GCCGGTCGCA CACAAGGAGA TGAGCGAGGA GTCGCGGGTT CGTCAGCGCT ACGTCGATTT GATCGTGCGT CCACAGGCGC GGGAGGTCGC GCGACAGCGG ATTGCGGTAA TCCGTGCGGT GCGTAATGCG CTGGAGCGGC GCGGGTTTCT CGAAGTCGAA ACGCCAATGT TGCAGACCTT GGCCGGCGGC GCTGCGGCGC GGCCGTTCGT CACCCATTCC AATGCCCTCG ACATCGATCT TTACCTGCGT ATCGCGCCGG AACTGTTCCT CAAGCGTTGC ATCGTGGGCG GTTTCGACAG GGTCTTTGAA CTAAATCGGG TGTTCCGAAA CGAAGGGTCT GATTCCACCC ATTCCCCGGA ATTCTCCATG CTGGAGACGT ATCAGACCTA TGGAACCTAT GACGATTCGG CCCTGATCAC CAGAGAGCTT ATTCAAGAAG TTGCTGACGA GGCGATCGGA ACCAGACAAC TCTCGATGCC TGACGGCAGC GTGTACGACA TCGACGGAGA ATGGGCAACT ATGGAGATGT ATTCCTCCTT GTCGGAGGCA CTGGGGGAAC AGATCACGCC AGAGACGACG GTTGCTCGCT TACGTGACAT CGCAAGCGGA CTTGACGTAG AGATTGACAA CAGTGTTTTT GGTCACGGCA AACTTGTCGA GGAACTCTGG GAACATGCTG TCGGCAACAA GTTGACGGCG CCCACATTTG TCAAGGATTT CCCAGTCGAG ACAACGCCAT TGACTCGTCA GCACCGCAGT ATCCCCGGAG TAACCGAGAA GTGGGATCTC TACGTGCGCG GAGTCGAACT GGCTACCGGG TACTCGGAAT TAAACGATCC CGTCGTCCAG CGGGACAGAT TCGCTGATCA GGCCCGTGCG GCGGCCGCGG GTGACGACGA GGCGATGCAA CTTGACGAAG ATTTTCTGAC GGCTCTGGAG TACGGGATGC CCCCGTGTAC TGGAACTGGA ATGGGCATCG ATCGGTTGTT GATGTGTTTG ACCGGACTGT CAATTAGGGA GACAGTTTTG TTCCCGATTG TTCGCCCTCA CTCCAACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT