MyulA.00587.f

Two component transcriptional regulatory protein TcrX

CENTER ID: MyulA.00587.f
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00587.f.A1.GE29714 Full length( MyulA.00587.f ) 1 234
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00587.f.A1.PS00800 Full length( MyulA.00587.f ) 1 234

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.5090
RefSeq: YP_907859.1
UniProt: A0PVL9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCRADGQPVS VLVVDDEPVL ADMVSMALRY EGWNIATASD GASAIASARA ERPDVVVLDV MLPDMSGLEV LHRLRKENPR LPVLLLTAKD AVEDRIAGLT AGGDDYVTKP FSIEEIVLRL RALLRRTGVT TVDSGAQLVV GDLVLDEDSH EVTRAGEPIS LTSTEFELLR FMMRNSKRVL SNAQILDRVW SYDFGGRSNI VELYISYLRK KIDNGREPMI HTLRGAGYVL KPAR
NT Sequence
ATGTGCCGGG CCGATGGTCA ACCGGTAAGC GTGTTGGTCG TCGATGACGA ACCCGTTCTG GCCGACATGG TGTCAATGGC ACTGCGTTAC GAGGGCTGGA ACATCGCCAC CGCCTCCGAT GGCGCGTCTG CGATCGCGTC CGCCCGCGCC GAGCGCCCTG ACGTCGTCGT ACTCGACGTG ATGCTGCCCG ACATGAGCGG GCTGGAAGTC CTGCATCGCT TGCGTAAGGA GAACCCACGG CTGCCGGTGC TGCTGTTGAC GGCCAAGGAT GCGGTGGAGG ATCGCATCGC CGGGTTGACC GCTGGCGGAG ACGATTACGT GACCAAGCCG TTCAGCATCG AGGAGATTGT GCTGCGGCTG CGGGCGCTGT TGCGACGCAC CGGTGTGACC ACCGTGGACA GCGGGGCTCA GCTGGTCGTC GGGGATCTGG TGCTCGATGA GGACAGTCAT GAAGTGACAC GCGCCGGCGA GCCGATTTCG TTGACATCAA CCGAATTCGA ATTGCTACGG TTCATGATGC GTAACTCCAA GCGGGTACTG AGTAATGCGC AGATTCTGGA CCGGGTATGG AGTTATGACT TCGGCGGCCG GTCCAACATC GTCGAGCTCT ACATCTCCTA CCTGCGCAAG AAGATCGACA ACGGTCGCGA ACCCATGATT CACACGCTGC GCGGCGCAGG TTATGTCCTC AAACCGGCTC GT
Details for MyulA.00587.f.A1.GE29714
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 28kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCRADGQPV SVLVVDDEPV LADMVSMALR YEGWNIATAS DGASAIASAR AERPDVVVLD VMLPDMSGLE VLHRLRKENP RLPVLLLTAK DAVEDRIAGL TAGGDDYVTK PFSIEEIVLR LRALLRRTGV TTVDSGAQLV VGDLVLDEDS HEVTRAGEPI SLTSTEFELL RFMMRNSKRV LSNAQILDRV WSYDFGGRSN IVELYISYLR KKIDNGREPM IHTLRGAGYV LKPAR
Validated NT Sequence
ttattaacga gccggtttga ggacataacc tgcgccgcgc agcgtgtgaa tcatgggttc gcgaccgttg tcgatcttct tgcgcaggta ggagatgtag agctcgacga tgttggaccg gccgccgaag tcataactcc atacccggtc cagaatctgc gcattactca gtacccgctt ggagttacgc atcatgaacc gtagcaattc gaattcggtt gatgtcaacg aaatcggctc gccggcgcgt gtcacttcat gactgtcctc atcgagcacc agatccccga cgaccagctg agccccgctg tccacggtgg tcacaccggt gcgtcgcaac agcgcccgca gccgcagcac aatctcctcg atgctgaacg gcttggtcac gtaatcgtct ccgccagcgg tcaacccggc gatgcgatcc tccaccgcat ccttggccgt caacagcagc accggcagcc gtgggttctc cttacgcaag cgatgcagga cttccagccc gctcatgtcg ggcagcatca cgtcgagtac gacgacgtca gggcgctcgg cgcgggcgga cgcgatcgca gacgcgccat cggaggcggt ggcgatgttc cagccctcgt aacgcagtgc cattgacacc atgtcggcca gaacgggttc gtcatcgacg accaacacgc ttaccggttg accatcggcc cggcacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCRADGQPV SVLVVDDEPV LADMVSMALR YEGWNIATAS DGASAIASAR AERPDVVVLD VMLPDMSGLE VLHRLRKENP RLPVLLLTAK DAVEDRIAGL TAGGDDYVTK PFSIEEIVLR LRALLRRTGV TTVDSGAQLV VGDLVLDEDS HEVTRAGEPI SLTSTEFELL RFMMRNSKRV LSNAQILDRV WSYDFGGRSN IVELYISYLR KKIDNGREPM IHTLRGAGYV LKPAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgccgggcc gatggtcaac cggtaagcgt gttggtcgtc gatgacgaac ccgttctggc cgacatggtg tcaatggcac tgcgttacga gggctggaac atcgccaccg cctccgatgg cgcgtctgcg atcgcgtccg cccgcgccga gcgccctgac gtcgtcgtac tcgacgtgat gctgcccgac atgagcgggc tggaagtcct gcatcgcttg cgtaaggaga acccacggct gccggtgctg ctgttgacgg ccaaggatgc ggtggaggat cgcatcgccg ggttgaccgc tggcggagac gattacgtga ccaagccgtt cagcatcgag gagattgtgc tgcggctgcg ggcgctgttg cgacgcaccg gtgtgaccac cgtggacagc ggggctcagc tggtcgtcgg ggatctggtg ctcgatgagg acagtcatga agtgacacgc gccggcgagc cgatttcgtt gacatcaacc gaattcgaat tgctacggtt catgatgcgt aactccaagc gggtactgag taatgcgcag attctggacc gggtatggag ttatgacttc ggcggccggt ccaacatcgt cgagctctac atctcctacc tgcgcaagaa gatcgacaac ggtcgcgaac ccatgattca cacgctgcgc ggcgcaggtt atgtcctcaa accggctcgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyulA.00587.f.A1.PS00800
PURIFICATION DATe: 10/11/2010
CONCENTRATION: 29.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCRADGQPV SVLVVDDEPV LADMVSMALR YEGWNIATAS DGASAIASAR AERPDVVVLD VMLPDMSGLE VLHRLRKENP RLPVLLLTAK DAVEDRIAGL TAGGDDYVTK PFSIEEIVLR LRALLRRTGV TTVDSGAQLV VGDLVLDEDS HEVTRAGEPI SLTSTEFELL RFMMRNSKRV LSNAQILDRV WSYDFGGRSN IVELYISYLR KKIDNGREPM IHTLRGAGYV LKPAR
Validated NT Sequence
ttattaacga gccggtttga ggacataacc tgcgccgcgc agcgtgtgaa tcatgggttc gcgaccgttg tcgatcttct tgcgcaggta ggagatgtag agctcgacga tgttggaccg gccgccgaag tcataactcc atacccggtc cagaatctgc gcattactca gtacccgctt ggagttacgc atcatgaacc gtagcaattc gaattcggtt gatgtcaacg aaatcggctc gccggcgcgt gtcacttcat gactgtcctc atcgagcacc agatccccga cgaccagctg agccccgctg tccacggtgg tcacaccggt gcgtcgcaac agcgcccgca gccgcagcac aatctcctcg atgctgaacg gcttggtcac gtaatcgtct ccgccagcgg tcaacccggc gatgcgatcc tccaccgcat ccttggccgt caacagcagc accggcagcc gtgggttctc cttacgcaag cgatgcagga cttccagccc gctcatgtcg ggcagcatca cgtcgagtac gacgacgtca gggcgctcgg cgcgggcgga cgcgatcgca gacgcgccat cggaggcggt ggcgatgttc cagccctcgt aacgcagtgc cattgacacc atgtcggcca gaacgggttc gtcatcgacg accaacacgc ttaccggttg accatcggcc cggcacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCRADGQPV SVLVVDDEPV LADMVSMALR YEGWNIATAS DGASAIASAR AERPDVVVLD VMLPDMSGLE VLHRLRKENP RLPVLLLTAK DAVEDRIAGL TAGGDDYVTK PFSIEEIVLR LRALLRRTGV TTVDSGAQLV VGDLVLDEDS HEVTRAGEPI SLTSTEFELL RFMMRNSKRV LSNAQILDRV WSYDFGGRSN IVELYISYLR KKIDNGREPM IHTLRGAGYV LKPAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCCGGGCC GATGGTCAAC CGGTAAGCGT GTTGGTCGTC GATGACGAAC CCGTTCTGGC CGACATGGTG TCAATGGCAC TGCGTTACGA GGGCTGGAAC ATCGCCACCG CCTCCGATGG CGCGTCTGCG ATCGCGTCCG CCCGCGCCGA GCGCCCTGAC GTCGTCGTAC TCGACGTGAT GCTGCCCGAC ATGAGCGGGC TGGAAGTCCT GCATCGCTTG CGTAAGGAGA ACCCACGGCT GCCGGTGCTG CTGTTGACGG CCAAGGATGC GGTGGAGGAT CGCATCGCCG GGTTGACCGC TGGCGGAGAC GATTACGTGA CCAAGCCGTT CAGCATCGAG GAGATTGTGC TGCGGCTGCG GGCGCTGTTG CGACGCACCG GTGTGACCAC CGTGGACAGC GGGGCTCAGC TGGTCGTCGG GGATCTGGTG CTCGATGAGG ACAGTCATGA AGTGACACGC GCCGGCGAGC CGATTTCGTT GACATCAACC GAATTCGAAT TGCTACGGTT CATGATGCGT AACTCCAAGC GGGTACTGAG TAATGCGCAG ATTCTGGACC GGGTATGGAG TTATGACTTC GGCGGCCGGT CCAACATCGT CGAGCTCTAC ATCTCCTACC TGCGCAAGAA GATCGACAAC GGTCGCGAAC CCATGATTCA CACGCTGCGC GGCGCAGGTT ATGTCCTCAA ACCGGCTCGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA