SSGCID: Target Page

MyulA.00556.a

Succinyl-CoA ligase [ADP-forming] subunit alpha

CENTER ID:	MyulA.00556.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00556.a.A1.GE29707	Full length( MyulA.00556.a )		1	300

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.5450
RefSeq:	YP_908120.1
UniProt:	A0PWD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIFLNKDNK VIVQGITGSE ATVHTARMLK AGTQIVGGVN ARKAGTTVTH EDKGGRVIKL PVFGGVAEAM EKTGADVSII FVPPKFAKDA IIEAIDAEIP LLVVITEVIP VQDTAYAWAY NLGKGGKTRI IGPNCPGIIT PGQALVGITP ANITGAGPVG LVSKSGTLTY QMMFELRDFG FTTSIGIGGD PVIGTTHIDA IEAFEKDPDT KLIVMIGEIG GDAEERAADF IKANVSKPVV GYVAGFTAPE GKTMGHAGAI VSGSSGTAAA KKEALEAAGV QVGKTPSETA VLAREILKGL

NT Sequence

ATGTCGATCT TCTTGAACAA GGACAACAAG GTCATCGTCC AGGGCATCAC CGGCAGTGAG GCGACCGTCC ACACTGCGCG CATGCTCAAG GCCGGCACCC AAATCGTCGG CGGGGTGAAC GCGCGCAAGG CGGGCACCAC CGTGACGCAC GAGGACAAGG GTGGTCGGGT AATCAAGCTG CCGGTATTCG GCGGTGTCGC CGAGGCGATG GAAAAGACCG GCGCCGACGT CTCGATCATC TTCGTGCCGC CGAAGTTCGC CAAGGACGCG ATCATCGAGG CCATTGACGC CGAGATTCCG CTGCTGGTGG TCATCACCGA GGTGATCCCG GTGCAAGACA CCGCCTATGC CTGGGCCTAC AACCTGGGCA AGGGCGGCAA GACCCGCATC ATCGGGCCCA ACTGCCCGGG CATCATCACC CCGGGGCAGG CGCTGGTTGG CATCACCCCG GCCAACATCA CCGGCGCCGG CCCGGTCGGG CTGGTCTCCA AGTCCGGCAC GCTGACCTAC CAGATGATGT TCGAGCTGCG CGATTTCGGT TTCACCACCT CGATCGGTAT CGGTGGCGAC CCAGTGATCG GCACCACCCA CATCGATGCC ATCGAGGCGT TCGAGAAGGA TCCCGACACC AAGCTGATCG TGATGATCGG TGAGATCGGT GGGGACGCCG AGGAGCGAGC GGCCGACTTC ATCAAGGCCA ACGTGTCCAA GCCGGTTGTC GGTTACGTGG CGGGATTCAC CGCGCCGGAG GGCAAGACCA TGGGGCACGC GGGTGCCATC GTGTCCGGCT CCTCGGGCAC CGCGGCGGCC AAGAAAGAAG CCCTGGAGGC CGCCGGGGTA CAGGTCGGCA AGACCCCGTC GGAGACCGCG GTGCTGGCCC GGGAGATCCT GAAGGGTCTC

Details for MyulA.00556.a.A1.GE29707

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/7/2010
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSIFLNKDN KVIVQGITGS EATVHTARML KAGTQIVGGV NARKAGTTVT HEDKGGRVIK LPVFGGVAEA MEKTGADVSI IFVPPKFAKD AIIEAIDAEI PLLVVITEVI PVQDTAYAWA YNLGKGGKTR IIGPNCPGII TPGQALVGIT PANITGAGPV GLVSKSGTLT YQMMFELRDF GFTTSIGIGG DPVIGTTHID AIEAFEKDPD TKLIVMIGEI GGDAEERAAD FIKANVSKPV VGYVAGFTAP EGKTMGHAGA IVSGSSGTAA AKKEALXAAG VQVGKTPSXX AXLARXILXX L

Validated NT Sequence

cnnnnnnntt ttgtttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgtcgatct tcttgaacaa ggacaacaag gtcatcgtcc agggcatcac cggcagtgag gcgaccgtcc acactgcgcg catgctcaag gccggcaccc aaatcgtcgg cggggtgaac gcgcgcaagg cgggcaccac cgtgacgcac gaggacaagg gtggtcgggt aatcaagctg ccggtattcg gcggtgtcgc cgaggcgatg gaaaagaccg gcgccgacgt ctcgatcatc ttcgtgccgc cgaagttcgc caaggacgcg atcatcgagg ccattgacgc cgagattccg ctgctggtgg tcatcaccga ggtgatcccg gtgcaagaca ccgcctatgc ctgggcctac aacctgggca agggcggcaa gacccgcatc atcgggccca actgcccggg catcatcacc ccggggcagg cgctggttgg catcaccccg gccaacatca ccggcgccgg cccggtcggg ctggtctcca agtccggcac gctgacctac cagatgatgt tcgagctgcg cgatttcggt ttcaccacct cgatcggtat cggtggcgac ccagtgatcg gcaccaccca catcgatgcc atcgaggcgt tcgagaagga tcccgacacc aagctgatcg tgatgatcgg tgagatcggt ggggacgccg aggagcgagc ggccgacttc atcaaggcca acgtgtccaa gccggttgtc ggttacgtgg cgggattcac cgcgccggag ggcaagacca tggggcacgc gggtgccatc gtgtccggct cctcgggcac cgcggcggcc aagaaagaag ccctgnnngc cgccggggta caggtcggca agaccccgtc gnanancgcg gngctggccc ggganatcct gnaggntctc taatnaanca cgaacantnt gcagccnnct nncgntccnn ctnctaacaa anccgaaang nanntnannn gntnntgc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgatcttc ttgaacaagg acaacaaggt catcgtccag ggcatcaccg gcagtgaggc gaccgtccac actgcgcgca tgctcaaggc cggcacccaa atcgtcggcg gggtgaacgc gcgcaaggcg ggcaccaccg tgacgcacga ggacaagggt ggtcgggtaa tcaagctgcc ggtattcggc ggtgtcgccg aggcgatgga aaagaccggc gccgacgtct cgatcatctt cgtgccgccg aagttcgcca aggacgcgat catcgaggcc attgacgccg agattccgct gctggtggtc atcaccgagg tgatcccggt gcaagacacc gcctatgcct gggcctacaa cctgggcaag ggcggcaaga cccgcatcat cgggcccaac tgcccgggca tcatcacccc ggggcaggcg ctggttggca tcaccccggc caacatcacc ggcgccggcc cggtcgggct ggtctccaag tccggcacgc tgacctacca gatgatgttc gagctgcgcg atttcggttt caccacctcg atcggtatcg gtggcgaccc agtgatcggc accacccaca tcgatgccat cgaggcgttc gagaaggatc ccgacaccaa gctgatcgtg atgatcggtg agatcggtgg ggacgccgag gagcgagcgg ccgacttcat caaggccaac gtgtccaagc cggttgtcgg ttacgtggcg ggattcaccg cgccggaggg caagaccatg gggcacgcgg gtgccatcgt gtccggctcc tcgggcaccg cggcggccaa gaaagaagcc ctggaggccg ccggggtaca ggtcggcaag accccgtcgg agaccgcggt gctggcccgg gagatcctga agggtctcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa