MyulA.00554.a
Short-chain type dehydrogenase/reductase
| CENTER ID: | MyulA.00554.a |
| ORGANISM: | Mycobacterium ulcerans Agy99 |
| ASSOCIATED DISEASE: | Buruli, Bairnsdale ulcer |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyulA.00554.a.A1.GE29706 | Full length( MyulA.00554.a ) | 1 | 253 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyulA.00554.a.A1.PS00974 | Full length( MyulA.00554.a ) | 1 | 253 |
Structures
3TL3
DEPOSITED: 8/29/2011
DETERMINATION: XRay
CLONE: MyulA.00554.a.A1.GE29706
PROTEIN: MyulA.00554.a.A1.PS00974
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|362242.7.peg.1222 |
| RefSeq: | YP_905071.1 |
| UniProt: | A0PMN1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEIRDAVAVV TGGASGLGLA TTKRLLDAGA QVVVLDIRGE DVVADLGDRA RFAAADVTDE AAVASALDLA ETMGTLRIVV NCAGTGNAIR VLSRDGVFSL AAFRKIVDIN LVGSFNVLRL AAERIAKTEP VGPNAEERGV IINTASVAAF DGQIGQAAYS ASKGGVVGMT LPIARDLASH RIRVMTIAPG LFDTPLLASL PEEARASLGK QVPHPSRLGN PDEYGALAVH IIENPMLNGE VIRLDGAIRM APR
NT Sequence
ATGGAGATCA GGGACGCCGT TGCCGTCGTC ACGGGGGGCG CGTCGGGGCT GGGCCTGGCG ACCACCAAGC GGCTGCTGGA CGCCGGGGCT CAGGTTGTGG TGCTCGACAT CCGGGGTGAA GACGTGGTTG CTGATTTGGG CGACCGGGCA CGGTTCGCGG CGGCGGATGT GACCGACGAG GCCGCCGTCG CCAGTGCGCT GGATCTGGCC GAAACGATGG GCACGCTGCG CATCGTCGTC AATTGCGCCG GGACCGGTAA CGCGATACGC GTGCTGAGCC GCGACGGCGT CTTCTCGTTG GCGGCGTTCC GCAAGATTGT GGACATCAAC CTGGTCGGGA GTTTTAACGT GCTGCGGCTG GCCGCCGAGC GAATCGCCAA AACCGAACCC GTCGGCCCCA ATGCGGAAGA GCGCGGCGTC ATCATCAACA CCGCCTCGGT GGCTGCATTC GATGGCCAGA TTGGCCAGGC TGCGTATTCG GCGTCCAAGG GCGGCGTGGT TGGTATGACC CTGCCGATCG CCCGCGATCT GGCCAGTCAT CGCATCCGGG TGATGACCAT CGCGCCCGGA CTGTTCGATA CCCCGCTGCT GGCGTCTTTG CCCGAGGAGG CCAGGGCGTC GCTGGGTAAG CAGGTGCCGC ACCCGTCCCG GCTGGGAAAC CCCGACGAAT ACGGGGCGCT GGCTGTGCAC ATCATTGAAA ACCCGATGCT CAACGGGGAG GTCATTCGTT TGGATGGCGC GATCCGGATG GCGCCGCGC
Details for MyulA.00554.a.A1.GE29706
| HARVESTED ON: | 6/29/2010 |
| SEQUENCED ON: | 7/7/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEIRDAVAV VTGGASGLGL ATTKRLLDAG AQVVVLDIRG EDVVADLGDR ARFAAADVTD EAAVASALDL AETMGTLRIV VNCAGTGNAI RVLSRDGVFS LAAFRKIVDI NLVGSFNVLR LAAERIAKTE PVGPNAEERG VIINTASVAA FDGQIGQAAY SASKGGVVGM TLPIARDLAS HRIRVMTIAP GLFDTPLLAS LPEEARASLG KQVPHPSRLG NPDEYGALAV HIIENPMLNG EVIRLDGAIR MAPR
Validated NT Sequence
ttattagcgc ggcgccatcc ggatcgcgcc atccaaacga atgacctccc cgttgagcat cgggttttca atgatgtgca cagccagcgc cccgtattcg tcggggtttc ccagccggga cgggtgcggc acctgcttac ccagcgacgc cctggcctcc tcgggcaaag acgccagcag cggggtatcg aacagtccgg gcgcgatggt catcacccgg atgcgatgac tggccagatc gcgggcgatc ggcagggtca taccaaccac gccgcccttg gacgccgaat acgcagcctg gccaatctgg ccatcgaatg cagccaccga ggcggtgttg atgatgacgc cgcgctcttc cgcattgggg ccgacgggtt cggttttggc gattcgctcg gcggccagcc gcagcacgtt aaaactcccg accaggttga tgtccacaat cttgcggaac gccgccaacg agaagacgcc gtcgcggctc agcacgcgta tcgcgttacc ggtcccggcg caattgacga cgatgcgcag cgtgcccatc gtttcggcca gatccagcgc actggcgacg gcggcctcgt cggtcacatc cgccgccgcg aaccgtgccc ggtcgcccaa atcagcaacc acgtcttcac cccggatgtc gagcaccaca acctgagccc cggcgtccag cagccgcttg gtggtcgcca ggcccagccc cgacgcgccc cccgtgacga cggcaacggc gtccctgatc tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEIRDAVAV VTGGASGLGL ATTKRLLDAG AQVVVLDIRG EDVVADLGDR ARFAAADVTD EAAVASALDL AETMGTLRIV VNCAGTGNAI RVLSRDGVFS LAAFRKIVDI NLVGSFNVLR LAAERIAKTE PVGPNAEERG VIINTASVAA FDGQIGQAAY SASKGGVVGM TLPIARDLAS HRIRVMTIAP GLFDTPLLAS LPEEARASLG KQVPHPSRLG NPDEYGALAV HIIENPMLNG EVIRLDGAIR MAPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagatcagg gacgccgttg ccgtcgtcac ggggggcgcg tcggggctgg gcctggcgac caccaagcgg ctgctggacg ccggggctca ggttgtggtg ctcgacatcc ggggtgaaga cgtggttgct gatttgggcg accgggcacg gttcgcggcg gcggatgtga ccgacgaggc cgccgtcgcc agtgcgctgg atctggccga aacgatgggc acgctgcgca tcgtcgtcaa ttgcgccggg accggtaacg cgatacgcgt gctgagccgc gacggcgtct tctcgttggc ggcgttccgc aagattgtgg acatcaacct ggtcgggagt tttaacgtgc tgcggctggc cgccgagcga atcgccaaaa ccgaacccgt cggccccaat gcggaagagc gcggcgtcat catcaacacc gcctcggtgg ctgcattcga tggccagatt ggccaggctg cgtattcggc gtccaagggc ggcgtggttg gtatgaccct gccgatcgcc cgcgatctgg ccagtcatcg catccgggtg atgaccatcg cgcccggact gttcgatacc ccgctgctgg cgtctttgcc cgaggaggcc agggcgtcgc tgggtaagca ggtgccgcac ccgtcccggc tgggaaaccc cgacgaatac ggggcgctgg ctgtgcacat cattgaaaac ccgatgctca acggggaggt cattcgtttg gatggcgcga tccggatggc gccgcgcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MyulA.00554.a.A1.PS00974
| PURIFICATION DATe: | 2/15/2011 |
| CONCENTRATION: | 25.7mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEIRDAVAV VTGGASGLGL ATTKRLLDAG AQVVVLDIRG EDVVADLGDR ARFAAADVTD EAAVASALDL AETMGTLRIV VNCAGTGNAI RVLSRDGVFS LAAFRKIVDI NLVGSFNVLR LAAERIAKTE PVGPNAEERG VIINTASVAA FDGQIGQAAY SASKGGVVGM TLPIARDLAS HRIRVMTIAP GLFDTPLLAS LPEEARASLG KQVPHPSRLG NPDEYGALAV HIIENPMLNG EVIRLDGAIR MAPR
Validated NT Sequence
ttattagcgc ggcgccatcc ggatcgcgcc atccaaacga atgacctccc cgttgagcat cgggttttca atgatgtgca cagccagcgc cccgtattcg tcggggtttc ccagccggga cgggtgcggc acctgcttac ccagcgacgc cctggcctcc tcgggcaaag acgccagcag cggggtatcg aacagtccgg gcgcgatggt catcacccgg atgcgatgac tggccagatc gcgggcgatc ggcagggtca taccaaccac gccgcccttg gacgccgaat acgcagcctg gccaatctgg ccatcgaatg cagccaccga ggcggtgttg atgatgacgc cgcgctcttc cgcattgggg ccgacgggtt cggttttggc gattcgctcg gcggccagcc gcagcacgtt aaaactcccg accaggttga tgtccacaat cttgcggaac gccgccaacg agaagacgcc gtcgcggctc agcacgcgta tcgcgttacc ggtcccggcg caattgacga cgatgcgcag cgtgcccatc gtttcggcca gatccagcgc actggcgacg gcggcctcgt cggtcacatc cgccgccgcg aaccgtgccc ggtcgcccaa atcagcaacc acgtcttcac cccggatgtc gagcaccaca acctgagccc cggcgtccag cagccgcttg gtggtcgcca ggcccagccc cgacgcgccc cccgtgacga cggcaacggc gtccctgatc tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEIRDAVAV VTGGASGLGL ATTKRLLDAG AQVVVLDIRG EDVVADLGDR ARFAAADVTD EAAVASALDL AETMGTLRIV VNCAGTGNAI RVLSRDGVFS LAAFRKIVDI NLVGSFNVLR LAAERIAKTE PVGPNAEERG VIINTASVAA FDGQIGQAAY SASKGGVVGM TLPIARDLAS HRIRVMTIAP GLFDTPLLAS LPEEARASLG KQVPHPSRLG NPDEYGALAV HIIENPMLNG EVIRLDGAIR MAPR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAGATCAGG GACGCCGTTG CCGTCGTCAC GGGGGGCGCG TCGGGGCTGG GCCTGGCGAC CACCAAGCGG CTGCTGGACG CCGGGGCTCA GGTTGTGGTG CTCGACATCC GGGGTGAAGA CGTGGTTGCT GATTTGGGCG ACCGGGCACG GTTCGCGGCG GCGGATGTGA CCGACGAGGC CGCCGTCGCC AGTGCGCTGG ATCTGGCCGA AACGATGGGC ACGCTGCGCA TCGTCGTCAA TTGCGCCGGG ACCGGTAACG CGATACGCGT GCTGAGCCGC GACGGCGTCT TCTCGTTGGC GGCGTTCCGC AAGATTGTGG ACATCAACCT GGTCGGGAGT TTTAACGTGC TGCGGCTGGC CGCCGAGCGA ATCGCCAAAA CCGAACCCGT CGGCCCCAAT GCGGAAGAGC GCGGCGTCAT CATCAACACC GCCTCGGTGG CTGCATTCGA TGGCCAGATT GGCCAGGCTG CGTATTCGGC GTCCAAGGGC GGCGTGGTTG GTATGACCCT GCCGATCGCC CGCGATCTGG CCAGTCATCG CATCCGGGTG ATGACCATCG CGCCCGGACT GTTCGATACC CCGCTGCTGG CGTCTTTGCC CGAGGAGGCC AGGGCGTCGC TGGGTAAGCA GGTGCCGCAC CCGTCCCGGC TGGGAAACCC CGACGAATAC GGGGCGCTGG CTGTGCACAT CATTGAAAAC CCGATGCTCA ACGGGGAGGT CATTCGTTTG GATGGCGCGA TCCGGATGGC GCCGCGCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA