SSGCID: Target Page

MyulA.00458.a

Acyl-CoA thioesterase II TesB1

CENTER ID:	MyulA.00458.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00458.a.A1.GE29696	Full length( MyulA.00458.a )		1	293

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.1918
RefSeq:	YP_905575.1
UniProt:	A0PP35

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRDEAKSDFE ELLAVLDLKR VADDRFIGAH PSKNPMRTFG GLLMAQSFVA NSRTLLRDDL PPSALSVHFI NGGDTTKDIE FQVVRLRDER RFANRRIDAV QNGTLLSSAM ISYMSGGRGL EHGVEPPPVA DPETRPPIGE LLRGYEETVP HFVNALQPIE WRYTNDPSWV MRDKGERLHY NRVWVKALGK LPDDPILHTA TMLYSSDTTV LDSVITTHGL SWGFDRIFAA SANHSVWFHR QVDFNDWVLY STSSPVAADS RGLGTGHFFN RTGQPVATVV QEGVLKYFPA ARR

NT Sequence

GTGCGAGACG AAGCCAAGTC GGACTTCGAG GAACTGCTCG CGGTCCTAGA CCTCAAACGC GTCGCGGATG ATCGGTTCAT CGGGGCCCAT CCCAGCAAGA ACCCGATGCG AACATTCGGT GGGCTGCTGA TGGCTCAGTC ATTTGTTGCC AACAGCCGCA CGTTGCTTCG AGACGATCTA CCGCCCAGCG CGCTCTCGGT GCACTTCATC AACGGTGGCG ACACGACGAA AGACATCGAA TTCCAGGTGG TGCGGCTGCG CGACGAGCGT CGTTTCGCCA ATCGACGGAT CGACGCGGTG CAGAATGGGA CGCTGCTGTC CTCGGCGATG ATCTCCTACA TGTCGGGCGG GCGTGGGCTC GAGCATGGTG TCGAGCCGCC GCCGGTGGCC GATCCAGAAA CTCGCCCGCC CATTGGCGAG CTGCTGCGCG GCTACGAGGA GACCGTTCCG CACTTCGTCA ACGCGCTGCA GCCGATCGAA TGGCGCTACA CCAACGATCC GTCCTGGGTG ATGCGTGACA AAGGGGAGCG GCTGCACTAC AACCGGGTCT GGGTAAAGGC TTTGGGTAAA CTGCCCGACG ATCCGATCCT GCACACCGCG ACGATGCTGT ATTCCTCGGA TACCACCGTG CTGGACTCGG TGATCACCAC CCACGGCCTG TCCTGGGGGT TTGACCGCAT TTTCGCCGCC TCGGCCAACC ATTCGGTGTG GTTTCACCGG CAGGTCGACT TCAACGATTG GGTGCTGTAT TCCACATCGT CACCGGTGGC GGCAGACTCA CGCGGCCTGG GCACTGGGCA CTTCTTCAAT CGCACCGGCC AGCCGGTGGC GACGGTGGTG CAGGAAGGCG TGCTGAAGTA TTTCCCCGCC GCACGACGA

Details for MyulA.00458.a.A1.GE29696

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/7/2010
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	107

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVRDEAKSD FEELLAVLDL KRVADDRFIG AHPSKNPMRT FGGLLMAQSF VANSRTLLRD DLPPSALSVH FINGGDTTKD IEFQVVRLRD ERRFANRRID AVQNGTLLSS AMISYMSGGR GLEHGVEPPP VADPETRPPI GELLRGYEET VPHFVNALQP IEWRYTNDPS WVMRDKGERL HYNRVWVKAL GKLPDDPILH TATMLYSSDT TVLDSVITTH GLSWGFDRIF AASANHSVWF HRQVDFNDWV LYSTSSPVAA DSRGLGTGHF FNRTGQPVAT VXQEXVLKYF PAARRXKQHE XVLXPSXRXX AAXXPKXS

Validated NT Sequence

ttntttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgcga gacgaagcca agtcggactt cgaggaactg ctcgcggtcc tagacctcaa acgcgtcgcg gatgatcggt tcatcggggc ccatcccagc aagaacccga tgcgaacatt cggtgggctg ctgatggctc agtcatttgt tgccaacagc cgcacgttgc ttcgagacga tctaccgccc agcgcgctct cggtgcactt catcaacggt ggcgacacga cgaaagacat cgaattccag gtggtgcggc tgcgcgacga gcgtcgtttc gccaatcgac ggatcgacgc ggtgcagaat gggacgctgc tgtcctcggc gatgatctcc tacatgtcgg gcgggcgtgg gctcgagcat ggtgtcgagc cgccgccggt ggccgatcca gaaactcgcc cgcccattgg cgagctgctg cgcggctacg aggagaccgt tccgcacttc gtcaacgcgc tgcagccgat cgaatggcgc tacaccaacg atccgtcctg ggtgatgcgt gacaaagggg agcggctgca ctacaaccgg gtctgggtaa aggctttggg taaactgccc gacgatccga tcctgcacac cgcgacgatg ctgtattcct cggataccac cgtgctggac tcggtgatca ccacccacgg cctgtcctgg gggtttgacc gcattttcgc cgcctcggcc aaccattcgg tgtggtttca ccggcaggtc gacttcaacg attgggtgct gtattccaca tcgtcaccgg tggcggcaga ctcacgcggc ctgggcactg ggcacttctt caatcgcacc ggccagccgg tggcgacggt ggngcaggaa ngcgtgctga agtatttccc cgccgcacga cganataaac agcacgaana agttctgcan ccaagctnnc gagnnncngc tgctnncaan ccgaaangaa gctgannnng ctgctgncnc nnnnnnnnnn nnnnnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgagac gaagccaagt cggacttcga ggaactgctc gcggtcctag acctcaaacg cgtcgcggat gatcggttca tcggggccca tcccagcaag aacccgatgc gaacattcgg tgggctgctg atggctcagt catttgttgc caacagccgc acgttgcttc gagacgatct accgcccagc gcgctctcgg tgcacttcat caacggtggc gacacgacga aagacatcga attccaggtg gtgcggctgc gcgacgagcg tcgtttcgcc aatcgacgga tcgacgcggt gcagaatggg acgctgctgt cctcggcgat gatctcctac atgtcgggcg ggcgtgggct cgagcatggt gtcgagccgc cgccggtggc cgatccagaa actcgcccgc ccattggcga gctgctgcgc ggctacgagg agaccgttcc gcacttcgtc aacgcgctgc agccgatcga atggcgctac accaacgatc cgtcctgggt gatgcgtgac aaaggggagc ggctgcacta caaccgggtc tgggtaaagg ctttgggtaa actgcccgac gatccgatcc tgcacaccgc gacgatgctg tattcctcgg ataccaccgt gctggactcg gtgatcacca cccacggcct gtcctggggg tttgaccgca ttttcgccgc ctcggccaac cattcggtgt ggtttcaccg gcaggtcgac ttcaacgatt gggtgctgta ttccacatcg tcaccggtgg cggcagactc acgcggcctg ggcactgggc acttcttcaa tcgcaccggc cagccggtgg cgacggtggt gcaggaaggc gtgctgaagt atttccccgc cgcacgacga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa