SSGCID: Target Page

MyulA.00416.b

Conserved ATP-binding protein ABC transporter

CENTER ID:	MyulA.00416.b
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00416.b.A1.GE29693	Full length( MyulA.00416.b )		1	280

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.2260
RefSeq:	YP_905828.1
UniProt:	A0PPT8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEAGPDTAD PDLLIDFRDV SLRRGGRTLV GPLDWAVELD ERWVILGPNG AGKTSLLRMA AASEHPSSGV AFVLGERLGR VDVSELRARI GLSSSALAQR IPTDEVVRDL VVSAGYAVLG RWRERYEDVD YRRAVDMLES LGAEHLADRT YGTLSEGERK RVLIARALMT DPELLLLDEP AAGLDLGGRE ELVARLADLA ADPDAPALVL VTHHVEEVPP GFSHCLLLAE GHVVASGLLS DVLTAENLWA AFGQQIAVDF VDGRYFARRI RARAAHRRQQ

NT Sequence

GTGTCTGAAG CTGGGCCTGA TACAGCCGAT CCCGACCTGT TGATCGACTT CAGAGACGTT TCGCTGCGTC GCGGCGGACG CACCTTGGTC GGTCCGCTGG ACTGGGCCGT CGAACTCGAC GAGCGTTGGG TGATCCTGGG CCCCAACGGC GCCGGCAAGA CCTCCTTGCT TCGGATGGCC GCCGCCTCCG AGCACCCGTC CTCGGGGGTT GCCTTCGTCC TGGGCGAACG GCTGGGCCGG GTCGACGTGT CGGAGCTGCG CGCGCGCATC GGGCTGAGTT CCTCGGCCCT GGCGCAGCGC ATACCCACCG ACGAAGTGGT GCGAGACCTC GTCGTATCGG CCGGCTACGC GGTGTTGGGC AGGTGGCGGG AGCGCTACGA GGACGTCGAC TACCGCCGGG CCGTCGACAT GCTGGAAAGC CTGGGCGCCG AGCATCTGGC CGACCGCACC TACGGAACCC TGTCCGAGGG GGAGCGCAAG CGGGTCTTGA TCGCCCGTGC GTTGATGACC GACCCCGAAC TGTTGCTGCT CGATGAGCCC GCGGCCGGTC TCGACTTGGG CGGCCGCGAG GAATTGGTGG CGCGGCTGGC CGATCTGGCC GCCGACCCGG ATGCGCCCGC CCTGGTGCTG GTCACCCACC ATGTCGAAGA GGTTCCTCCC GGCTTCAGCC ACTGCCTGCT GCTGGCGGAG GGCCACGTCG TGGCATCGGG CCTATTGTCC GACGTGCTCA CCGCCGAAAA TCTGTGGGCC GCATTCGGCC AACAGATTGC CGTCGATTTC GTCGACGGAC GATATTTCGC CCGCCGGATC CGCGCCCGCG CAGCCCACCG GAGGCAGCAA

Details for MyulA.00416.b.A1.GE29693

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/7/2010
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMVSEAGP DTADPDLLID FRDVSLRRGG RTLVGPLDWA VELDERWVIL GPNGAGKTSL LRMAAASEHP SSGVAFVLGE RLGRVDVSEL RARIGLSSSA LAQRIPTDEV VRDLVVSAGY AVLGRWRERY EDVDYRRAVD MLESLGAEHL ADRTYGTLSE GERKRVLIAR ALMTDPELLL LDEPAAGLDL GGREELVARL ADLAADPDAP ALVLVTHHVE EVPPGFSHCL LLAEGHVVAS GLLSDVLTAE NLWAAFGQQI AVDFVDGRYF ARRIRARAAH R

Validated NT Sequence

cctccggtgg gctgcgcggg cgcggatccg gcgggcgaaa tatcgtccgt cgacgaaatc gacggcaatc tgttggccga atgcggccca cagattttcg gcggtgagca cgtcggacaa taggcccgat gccacgacgt ggccctccgc cagcagcagg cagtggctga agccgggagg aacctcttcg acatggtggg tgaccagcac cagggcgggc gcatccgggt cggcggccag atcggccagc cgcgccacca attcctcgcg gccgcccaag tcgagaccgg ccgcgggctc atcgagcagc aacagttcgg ggtcggtcat caacgcacgg gcgatcaaga cccgcttgcg ctccccctcg gacagggttc cgtaggtgcg gtcggccaga tgctcggcgc ccaggctttc cagcatgtcg acggcccggc ggtagtcgac gtcctcgtag cgctcccgcc acctgcccaa caccgcgtag ccggccgata cgacgaggtc tcgcaccact tcgtcggtgg gtatgcgctg cgccagggcc gaggaactca gcccgatgcg cgcgcgcagc tccgacacgt cgacccggcc cagccgttcg cccaggacga aggcaacccc cgaggacggg tgctcggagg cggcggccat ccgaagcaag gaggtcttgc cggcgccgtt ggggcccagg atcacccaac gctcgtcgag ttcgacggcc cagtccagcg gaccgaccaa ggtgcgtccg ccgcgacgca gcgaaacgtc tctgaagtcg atcaacaggt cgggatcggc tgtatcaggc ccagcttcag acaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSEAGPDT ADPDLLIDFR DVSLRRGGRT LVGPLDWAVE LDERWVILGP NGAGKTSLLR MAAASEHPSS GVAFVLGERL GRVDVSELRA RIGLSSSALA QRIPTDEVVR DLVVSAGYAV LGRWRERYED VDYRRAVDML ESLGAEHLAD RTYGTLSEGE RKRVLIARAL MTDPELLLLD EPAAGLDLGG REELVARLAD LAADPDAPAL VLVTHHVEEV PPGFSHCLLL AEGHVVASGL LSDVLTAENL WAAFGQQIAV DFVDGRYFAR RIRARAAHRR QQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtctgaa gctgggcctg atacagccga tcccgacctg ttgatcgact tcagagacgt ttcgctgcgt cgcggcggac gcaccttggt cggtccgctg gactgggccg tcgaactcga cgagcgttgg gtgatcctgg gccccaacgg cgccggcaag acctccttgc ttcggatggc cgccgcctcc gagcacccgt cctcgggggt tgccttcgtc ctgggcgaac ggctgggccg ggtcgacgtg tcggagctgc gcgcgcgcat cgggctgagt tcctcggccc tggcgcagcg catacccacc gacgaagtgg tgcgagacct cgtcgtatcg gccggctacg cggtgttggg caggtggcgg gagcgctacg aggacgtcga ctaccgccgg gccgtcgaca tgctggaaag cctgggcgcc gagcatctgg ccgaccgcac ctacggaacc ctgtccgagg gggagcgcaa gcgggtcttg atcgcccgtg cgttgatgac cgaccccgaa ctgttgctgc tcgatgagcc cgcggccggt ctcgacttgg gcggccgcga ggaattggtg gcgcggctgg ccgatctggc cgccgacccg gatgcgcccg ccctggtgct ggtcacccac catgtcgaag aggttcctcc cggcttcagc cactgcctgc tgctggcgga gggccacgtc gtggcatcgg gcctattgtc cgacgtgctc accgccgaaa atctgtgggc cgcattcggc caacagattg ccgtcgattt cgtcgacgga cgatatttcg cccgccggat ccgcgcccgc gcagcccacc ggaggcagca aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa