MyulA.00416.a

ATPase component of an ABC-type transport system

CENTER ID: MyulA.00416.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00416.a.A1.GE29692 Full length( MyulA.00416.a ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4822
RefSeq: YP_907659.1
UniProt: A0PV19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQSNHPAIA LRGARLAFGD RVLWEDLDLS VSRGEFIAVL GPNGTGKTSL LKVLLGELAL SAGVALVDGA RLDTGRTRRG HIGYVPQHHP IDREVMLRGR DLVGLGVDGC RWGAVALRSG ERARRREAVQ RALQQVGGER LADVRVGLMS GGELQRIRVA QALASDPSLL LCDEPLLALD PANANLVAAL LDRRRREAAT AVMVVTHEVN PILPYVDRVL YLVDGRFLIG TVEQVMTSQT LSTLYRADIE VIKVVKGPHT RYLVVDAQDG LR
NT Sequence
TTGAACCAGT CGAACCACCC GGCAATTGCG CTGCGCGGTG CGCGGCTGGC ATTCGGCGAT CGGGTCCTGT GGGAGGACCT CGATCTGTCG GTGTCACGCG GCGAATTCAT CGCCGTGCTC GGGCCCAACG GCACCGGCAA GACCTCGTTG CTCAAGGTGT TGCTCGGCGA ACTCGCGTTG AGCGCAGGTG TGGCGCTGGT GGACGGCGCC AGGCTCGACA CCGGTCGAAC GCGCCGCGGC CATATCGGCT ATGTCCCACA GCACCATCCG ATCGACCGGG AGGTGATGCT GCGCGGGCGC GACCTGGTCG GGCTGGGTGT CGACGGATGC CGGTGGGGTG CGGTTGCGTT GCGCTCGGGC GAGCGGGCCC GTCGCCGCGA AGCCGTGCAG CGGGCGCTGC AGCAGGTCGG CGGTGAGCGG CTTGCCGACG TCCGGGTGGG GCTGATGTCG GGGGGCGAGC TGCAGCGGAT ACGCGTCGCC CAGGCACTGG CAAGCGACCC GTCGTTGCTG CTGTGCGATG AACCGCTACT GGCCCTCGAC CCGGCCAACG CCAACCTGGT CGCCGCTCTG CTCGATCGCC GTCGCCGGGA GGCCGCAACC GCGGTCATGG TGGTCACCCA CGAGGTCAAC CCGATCTTGC CGTACGTGGA CCGGGTGCTC TACCTGGTCG ACGGGCGATT CTTGATCGGC ACCGTCGAGC AGGTGATGAC CAGCCAGACC CTCTCGACGC TGTACCGCGC CGATATCGAG GTGATCAAAG TCGTCAAGGG CCCCCACACC CGCTATCTGG TAGTCGACGC CCAGGACGGG CTCCGA
Details for MyulA.00416.a.A1.GE29692
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLNQSNHPA IALRGARLAF GDRVLWEDLD LSVSRGEFIA VLGPNGTGKT SLLKVLLGEL ALSAGVALVD GARLDTGRTR RGHIGYVPQH HPIDREVMLR GRDLVGLGVD GCRWGAVALR SGERARRREA VQRALQQVGG ERLADVRVGL MSGGELQRIR VAQALASDPS LLLCDEPLLA LDPANANLVA ALLDRRRREA ATAVMVVTHE VNPILPYVDR VLYLVDGRFL IGTVEQVMTS QTLSTLYRAD IEVIKVVKGP HTRYLVVDAX DGLR
Validated NT Sequence
ttttntttan tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgaa ccagtcgaac cacccggcaa ttgcgctgcg cggtgcgcgg ctggcattcg gcgatcgggt cctgtgggag gacctcgatc tgtcggtgtc acgcggcgaa ttcatcgccg tgctcgggcc caacggcacc ggcaagacct cgttgctcaa ggtgttgctc ggcgaactcg cgttgagcgc aggtgtggcg ctggtggacg gcgccaggct cgacaccggt cgaacgcgcc gcggccatat cggctatgtc ccacagcacc atccgatcga ccgggaggtg atgctgcgcg ggcgcgacct ggtcgggctg ggtgtcgacg gatgccggtg gggtgcggtt gcgttgcgct cgggcgagcg ggcccgtcgc cgcgaagccg tgcagcgggc gctgcagcag gtcggcggtg agcggcttgc cgacgtccgg gtggggctga tgtcgggggg cgagctgcag cggatacgcg tcgcccaggc actggcaagc gacccgtcgt tgctgctgtg cgatgaaccg ctactggccc tcgacccggc caacgccaac ctggtcgccg ctctgctcga tcgccgtcgc cgggaggccg caaccgcggt catggtggtc acccacgagg tcaacccgat cttgccgtac gtggaccggg tgctctacct ggtcgacggg cgattcttga tcggcaccgt cgagcaggtg atgaccagcc agaccctctc gacgctgtac cgcgccgata tcgaggtgat caaagtcgtc aagggccccc acacccgcta tctggtagtc gacgcccang acgggctccg ataataaaca gcacgaacaa gttnngcagc cnagcttctc gaggatncng ctgctaacaa agncngaaag gaagctgant tggntgctgc cnccgntnnn nnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLNQSNHPA IALRGARLAF GDRVLWEDLD LSVSRGEFIA VLGPNGTGKT SLLKVLLGEL ALSAGVALVD GARLDTGRTR RGHIGYVPQH HPIDREVMLR GRDLVGLGVD GCRWGAVALR SGERARRREA VQRALQQVGG ERLADVRVGL MSGGELQRIR VAQALASDPS LLLCDEPLLA LDPANANLVA ALLDRRRREA ATAVMVVTHE VNPILPYVDR VLYLVDGRFL IGTVEQVMTS QTLSTLYRAD IEVIKVVKGP HTRYLVVDAQ DGLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaaccag tcgaaccacc cggcaattgc gctgcgcggt gcgcggctgg cattcggcga tcgggtcctg tgggaggacc tcgatctgtc ggtgtcacgc ggcgaattca tcgccgtgct cgggcccaac ggcaccggca agacctcgtt gctcaaggtg ttgctcggcg aactcgcgtt gagcgcaggt gtggcgctgg tggacggcgc caggctcgac accggtcgaa cgcgccgcgg ccatatcggc tatgtcccac agcaccatcc gatcgaccgg gaggtgatgc tgcgcgggcg cgacctggtc gggctgggtg tcgacggatg ccggtggggt gcggttgcgt tgcgctcggg cgagcgggcc cgtcgccgcg aagccgtgca gcgggcgctg cagcaggtcg gcggtgagcg gcttgccgac gtccgggtgg ggctgatgtc ggggggcgag ctgcagcgga tacgcgtcgc ccaggcactg gcaagcgacc cgtcgttgct gctgtgcgat gaaccgctac tggccctcga cccggccaac gccaacctgg tcgccgctct gctcgatcgc cgtcgccggg aggccgcaac cgcggtcatg gtggtcaccc acgaggtcaa cccgatcttg ccgtacgtgg accgggtgct ctacctggtc gacgggcgat tcttgatcgg caccgtcgag caggtgatga ccagccagac cctctcgacg ctgtaccgcg ccgatatcga ggtgatcaaa gtcgtcaagg gcccccacac ccgctatctg gtagtcgacg cccaggacgg gctccgaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa