MyulA.00358.c

Enoyl-CoA hydratase EchA2

CENTER ID: MyulA.00358.c
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00358.c.A1.GE29682 Full length( MyulA.00358.c ) 1 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00358.c.A1.PS00768 Full length( MyulA.00358.c ) 1 304

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.1702
RefSeq: YP_905425.1
UniProt: A0PNN5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTPEFQTLL YSTAGPVATI TLNRPEHLNT IVPPMPDEIE AAVGLAERDQ GIKVIVLRGA GRAFSGGYDF GGGFQHWGET MMTDGQWDPG KDFAMVTARE TAPTQKFMAI WRASKPVIAQ IHGWCVGGAS DYALCADIVI ASDDAVIGTP YSRMWGAYLS GMWLYRLSFA KAKWHSLTGR PLSGVQAAQI ELINEAVPFE RLEARVAEVA AELAQIPLSQ LQAQKLIVNQ AYENMGLAST QTLGGILDGL MRNTPDALDF IKTAENQGVR AAVERRDGPF GDYSQAPPEL RPNPNHVIVP DRDR
NT Sequence
ATGCCAACTC CTGAGTTCCA GACGCTGCTG TACTCGACAG CGGGCCCGGT GGCGACCATC ACGCTCAATC GCCCGGAGCA CCTCAACACG ATCGTTCCGC CCATGCCCGA TGAGATCGAG GCAGCCGTGG GGCTGGCCGA ACGGGACCAG GGCATCAAAG TCATCGTGTT ACGCGGTGCC GGCCGCGCCT TCTCGGGGGG TTACGACTTT GGCGGGGGTT TCCAGCATTG GGGCGAAACC ATGATGACCG ACGGGCAATG GGATCCGGGC AAAGACTTCG CCATGGTCAC CGCCCGCGAG ACCGCCCCGA CACAGAAGTT CATGGCCATC TGGCGGGCGT CGAAACCGGT GATCGCGCAG ATCCACGGCT GGTGTGTCGG GGGAGCCAGC GACTACGCGT TGTGCGCCGA CATCGTCATT GCCAGCGATG ACGCCGTGAT CGGAACCCCG TACAGCCGAA TGTGGGGCGC GTACCTGTCC GGGATGTGGC TCTACCGGTT GAGCTTTGCC AAGGCCAAAT GGCATTCCTT GACCGGTCGG CCGCTGTCCG GCGTCCAGGC CGCGCAGATC GAGCTGATCA ACGAGGCGGT GCCGTTCGAA CGGCTCGAAG CCCGGGTCGC CGAGGTCGCC GCCGAGCTGG CGCAGATTCC GTTGTCCCAG CTACAGGCGC AGAAACTGAT CGTCAATCAG GCCTACGAAA ATATGGGGCT GGCATCCACC CAGACCCTGG GCGGCATTCT CGACGGCCTG ATGCGCAATA CGCCGGACGC GTTGGACTTC ATCAAAACCG CCGAAAACCA GGGAGTGCGG GCCGCGGTCG AGCGTCGAGA TGGACCGTTC GGTGATTACA GCCAGGCCCC ACCGGAGCTG CGGCCCAACC CCAATCACGT GATAGTCCCT GATCGCGACC GA
Details for MyulA.00358.c.A1.GE29682
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPTPEFQTL LYSTAGPVAT ITLNRPEHLN TIVPPMPDEI EAAVGLAERD QGIKVIVLRG AGRAFSGGYD FGGGFQHWGE TMMTDGQWDP GKDFAMVTAR ETAPTQKFMA IWRASKPVIA QIHGWCVGGA SDYALCADIV IASDDAVIGT PYSRMWGAYL SGMWLYRLSF AKAKWHSLTG RPLSGVQAAQ IELINEAVPF ERLEARVAEV AAELAQIPLS QLQAQKLIVN QAYENMGLAS TQTLGGILDG LMRNTPDALD FIKTAENQGV RAAVERRDGP FGDYSQAPPE LRPNPNHVIV PDR
Validated NT Sequence
cgcgatcagg gactatcacg tgattggggt tgggccgcag ctccggtggg gcctggctgt aatcaccgaa cggtccatct cgacgctcga ccgcggcccg cactccctgg ttttcggcgg ttttgatgaa gtccaacgcg tccggcgtat tgcgcatcag gccgtcgaga atgccgccca gggtctgggt ggatgccagc cccatatttt cgtaggcctg attgacgatc agtttctgcg cctgtagctg ggacaacgga atctgcgcca gctcggcggc gacctcggcg acccgggctt cgagccgttc gaacggcacc gcctcgttga tcagctcgat ctgcgcggcc tggacgccgg acagcggccg accggtcaag gaatgccatt tggccttggc aaagctcaac cggtagagcc acatcccgga caggtacgcg ccccacattc ggctgtacgg ggttccgatc acggcgtcat cgctggcaat gacgatgtcg gcgcacaacg cgtagtcgct ggctcccccg acacaccagc cgtggatctg cgcgatcacc ggtttcgacg cccgccagat ggccatgaac ttctgtgtcg gggcggtctc gcgggcggtg accatggcga agtctttgcc cggatcccat tgcccgtcgg tcatcatggt ttcgccccaa tgctggaaac ccccgccaaa gtcgtaaccc cccgagaagg cgcggccggc accgcgtaac acgatgactt tgatgccctg gtcccgttcg gccagcccca cggctgcctc gatctcatcg ggcatgggcg gaacgatcgt gttgaggtgc tccgggcgat tgagcgtgat ggtcgccacc gggcccgctg tcgagtacag cagcgtctgg aactcaggag ttggcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPTPEFQTL LYSTAGPVAT ITLNRPEHLN TIVPPMPDEI EAAVGLAERD QGIKVIVLRG AGRAFSGGYD FGGGFQHWGE TMMTDGQWDP GKDFAMVTAR ETAPTQKFMA IWRASKPVIA QIHGWCVGGA SDYALCADIV IASDDAVIGT PYSRMWGAYL SGMWLYRLSF AKAKWHSLTG RPLSGVQAAQ IELINEAVPF ERLEARVAEV AAELAQIPLS QLQAQKLIVN QAYENMGLAS TQTLGGILDG LMRNTPDALD FIKTAENQGV RAAVERRDGP FGDYSQAPPE LRPNPNHVIV PDRDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccaactcct gagttccaga cgctgctgta ctcgacagcg ggcccggtgg cgaccatcac gctcaatcgc ccggagcacc tcaacacgat cgttccgccc atgcccgatg agatcgaggc agccgtgggg ctggccgaac gggaccaggg catcaaagtc atcgtgttac gcggtgccgg ccgcgccttc tcggggggtt acgactttgg cgggggtttc cagcattggg gcgaaaccat gatgaccgac gggcaatggg atccgggcaa agacttcgcc atggtcaccg cccgcgagac cgccccgaca cagaagttca tggccatctg gcgggcgtcg aaaccggtga tcgcgcagat ccacggctgg tgtgtcgggg gagccagcga ctacgcgttg tgcgccgaca tcgtcattgc cagcgatgac gccgtgatcg gaaccccgta cagccgaatg tggggcgcgt acctgtccgg gatgtggctc taccggttga gctttgccaa ggccaaatgg cattccttga ccggtcggcc gctgtccggc gtccaggccg cgcagatcga gctgatcaac gaggcggtgc cgttcgaacg gctcgaagcc cgggtcgccg aggtcgccgc cgagctggcg cagattccgt tgtcccagct acaggcgcag aaactgatcg tcaatcaggc ctacgaaaat atggggctgg catccaccca gaccctgggc ggcattctcg acggcctgat gcgcaatacg ccggacgcgt tggacttcat caaaaccgcc gaaaaccagg gagtgcgggc cgcggtcgag cgtcgagatg gaccgttcgg tgattacagc caggccccac cggagctgcg gcccaacccc aatcacgtga tagtccctga tcgcgaccga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyulA.00358.c.A1.PS00768
PURIFICATION DATe: 9/14/2010
CONCENTRATION: 53.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPTPEFQTL LYSTAGPVAT ITLNRPEHLN TIVPPMPDEI EAAVGLAERD QGIKVIVLRG AGRAFSGGYD FGGGFQHWGE TMMTDGQWDP GKDFAMVTAR ETAPTQKFMA IWRASKPVIA QIHGWCVGGA SDYALCADIV IASDDAVIGT PYSRMWGAYL SGMWLYRLSF AKAKWHSLTG RPLSGVQAAQ IELINEAVPF ERLEARVAEV AAELAQIPLS QLQAQKLIVN QAYENMGLAS TQTLGGILDG LMRNTPDALD FIKTAENQGV RAAVERRDGP FGDYSQAPPE LRPNPNHVIV PDR
Validated NT Sequence
cgcgatcagg gactatcacg tgattggggt tgggccgcag ctccggtggg gcctggctgt aatcaccgaa cggtccatct cgacgctcga ccgcggcccg cactccctgg ttttcggcgg ttttgatgaa gtccaacgcg tccggcgtat tgcgcatcag gccgtcgaga atgccgccca gggtctgggt ggatgccagc cccatatttt cgtaggcctg attgacgatc agtttctgcg cctgtagctg ggacaacgga atctgcgcca gctcggcggc gacctcggcg acccgggctt cgagccgttc gaacggcacc gcctcgttga tcagctcgat ctgcgcggcc tggacgccgg acagcggccg accggtcaag gaatgccatt tggccttggc aaagctcaac cggtagagcc acatcccgga caggtacgcg ccccacattc ggctgtacgg ggttccgatc acggcgtcat cgctggcaat gacgatgtcg gcgcacaacg cgtagtcgct ggctcccccg acacaccagc cgtggatctg cgcgatcacc ggtttcgacg cccgccagat ggccatgaac ttctgtgtcg gggcggtctc gcgggcggtg accatggcga agtctttgcc cggatcccat tgcccgtcgg tcatcatggt ttcgccccaa tgctggaaac ccccgccaaa gtcgtaaccc cccgagaagg cgcggccggc accgcgtaac acgatgactt tgatgccctg gtcccgttcg gccagcccca cggctgcctc gatctcatcg ggcatgggcg gaacgatcgt gttgaggtgc tccgggcgat tgagcgtgat ggtcgccacc gggcccgctg tcgagtacag cagcgtctgg aactcaggag ttggcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPTPEFQTL LYSTAGPVAT ITLNRPEHLN TIVPPMPDEI EAAVGLAERD QGIKVIVLRG AGRAFSGGYD FGGGFQHWGE TMMTDGQWDP GKDFAMVTAR ETAPTQKFMA IWRASKPVIA QIHGWCVGGA SDYALCADIV IASDDAVIGT PYSRMWGAYL SGMWLYRLSF AKAKWHSLTG RPLSGVQAAQ IELINEAVPF ERLEARVAEV AAELAQIPLS QLQAQKLIVN QAYENMGLAS TQTLGGILDG LMRNTPDALD FIKTAENQGV RAAVERRDGP FGDYSQAPPE LRPNPNHVIV PDRDR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCAACTCCT GAGTTCCAGA CGCTGCTGTA CTCGACAGCG GGCCCGGTGG CGACCATCAC GCTCAATCGC CCGGAGCACC TCAACACGAT CGTTCCGCCC ATGCCCGATG AGATCGAGGC AGCCGTGGGG CTGGCCGAAC GGGACCAGGG CATCAAAGTC ATCGTGTTAC GCGGTGCCGG CCGCGCCTTC TCGGGGGGTT ACGACTTTGG CGGGGGTTTC CAGCATTGGG GCGAAACCAT GATGACCGAC GGGCAATGGG ATCCGGGCAA AGACTTCGCC ATGGTCACCG CCCGCGAGAC CGCCCCGACA CAGAAGTTCA TGGCCATCTG GCGGGCGTCG AAACCGGTGA TCGCGCAGAT CCACGGCTGG TGTGTCGGGG GAGCCAGCGA CTACGCGTTG TGCGCCGACA TCGTCATTGC CAGCGATGAC GCCGTGATCG GAACCCCGTA CAGCCGAATG TGGGGCGCGT ACCTGTCCGG GATGTGGCTC TACCGGTTGA GCTTTGCCAA GGCCAAATGG CATTCCTTGA CCGGTCGGCC GCTGTCCGGC GTCCAGGCCG CGCAGATCGA GCTGATCAAC GAGGCGGTGC CGTTCGAACG GCTCGAAGCC CGGGTCGCCG AGGTCGCCGC CGAGCTGGCG CAGATTCCGT TGTCCCAGCT ACAGGCGCAG AAACTGATCG TCAATCAGGC CTACGAAAAT ATGGGGCTGG CATCCACCCA GACCCTGGGC GGCATTCTCG ACGGCCTGAT GCGCAATACG CCGGACGCGT TGGACTTCAT CAAAACCGCC GAAAACCAGG GAGTGCGGGC CGCGGTCGAG CGTCGAGATG GACCGTTCGG TGATTACAGC CAGGCCCCAC CGGAGCTGCG GCCCAACCCC AATCACGTGA TAGTCCCTGA TCGCGACCGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA