MyulA.00309.b

Acyl-CoA dehydrogenase FadE1

CENTER ID: MyulA.00309.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00309.b.A1.GE29677 Full length( MyulA.00309.b ) 1 430
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.5533
RefSeq: YP_908175.1
UniProt: A0PWI5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWDFETDPEY QAKLDWVQEF MVNELEPLDL VALDPYDKKN PEMMAVLRPL QQVKNQGLWA AHLRPEVGGQ GFGQVKLALL NEILGRSRWA PSVFGCQAPD SGNAEILALF GTEDQKARYL QPLLDGEITS CYSMTEPQGG SDPGSFVTAA TRDGDSWIIN GEKWFSTNAK HASFFIVMAV TNPQARTYEK MSLFIVPAET PGIEIVRNVG VGTESSKHAS HGYVRYHDVR VPADHVLGGE GQAFMTAQTR LGGGRIHHAM RTIALARRAF DMMCERAVSR QTRHGRLSDL QMTQEKVADS WIQIEQFRLL VLRTAWLIDK HHDYQKVRRD IAAVKVAMPQ VLHDVVQRAM HLHGALGVSD EMPFVKMMVA AESLGIADGA TELHKMTVAR RTLRQYEPVA TPFPSQHIPT RRAAAEARLA ERLEHSIAEF
NT Sequence
GTGTGGGACT TCGAAACAGA CCCCGAATAC CAGGCGAAGC TGGACTGGGT CCAAGAGTTC ATGGTCAACG AACTCGAACC ACTGGATCTG GTAGCTCTCG ATCCCTATGA CAAGAAGAAC CCCGAGATGA TGGCCGTCCT TCGGCCGTTG CAGCAGGTGA AGAACCAGGG GCTCTGGGCC GCTCACCTGC GACCGGAAGT CGGCGGGCAG GGGTTTGGCC AAGTCAAGCT TGCGCTGCTG AACGAAATCC TCGGACGCTC GCGCTGGGCG CCCTCGGTGT TCGGTTGTCA GGCACCAGAC TCGGGTAACG CCGAGATCCT GGCGCTGTTC GGCACCGAGG ACCAGAAGGC CCGCTACCTG CAACCGCTGC TGGACGGCGA AATCACCTCC TGCTACTCGA TGACAGAACC TCAGGGAGGT TCGGATCCAG GGTCGTTCGT CACGGCCGCC ACCCGCGACG GAGACAGCTG GATCATCAAC GGGGAGAAGT GGTTTTCCAC CAACGCCAAG CACGCCTCGT TCTTCATCGT GATGGCCGTG ACCAACCCGC AGGCCCGCAC CTACGAGAAG ATGTCGCTGT TCATCGTCCC TGCGGAGACA CCCGGTATCG AGATCGTCCG CAACGTCGGC GTCGGGACCG AGTCGAGCAA GCACGCCAGC CACGGCTACG TCCGCTACCA CGATGTTCGG GTGCCGGCCG ATCATGTGCT CGGCGGTGAA GGCCAGGCGT TCATGACCGC GCAGACGCGA CTCGGCGGCG GTCGCATTCA CCACGCGATG CGCACAATTG CATTGGCACG ACGAGCCTTT GACATGATGT GCGAACGCGC CGTATCCAGG CAGACCAGAC ACGGCCGGCT TTCCGACTTG CAGATGACGC AGGAGAAGGT CGCCGACAGC TGGATCCAAA TCGAGCAGTT CCGGCTACTG GTGCTGCGCA CGGCGTGGCT GATCGACAAG CACCACGACT ATCAGAAGGT GCGTCGCGAC ATCGCGGCGG TGAAGGTCGC AATGCCACAG GTGCTGCACG ACGTGGTGCA GCGCGCCATG CACCTGCACG GCGCGCTCGG CGTTTCCGAC GAGATGCCGT TCGTCAAGAT GATGGTGGCC GCCGAATCGC TGGGCATCGC CGACGGCGCC ACCGAACTGC ACAAGATGAC GGTGGCACGC CGGACACTGC GCCAATACGA GCCTGTTGCA ACGCCGTTCC CGTCACAGCA CATACCCACC CGGCGAGCGG CGGCCGAAGC GCGGCTGGCC GAGCGCCTGG AACACAGCAT CGCGGAGTTC
Details for MyulA.00309.b.A1.GE29677
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 50kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 64
PERCENT COVERAGE: 79
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVWDFETDP EYQAKLDWVQ EFMVNELEPL DLVALDPYDK KNPEMMAVLR PLQQVKNQGL WAAHLRPEVG GQGFGQVKLA LLNEILGRSR WAPSVFGCQA PDSGNAEILA LFGTEDQKAR YLQPLLDGEI TSCYSMTEPX GGSDPGSFVT AATRDGXSWI INGEKWFSTN AKHASXFIVM AVTNPQARXY XKMSLFIVPA XTPXIEIVRN VXVXXXSSXX XXXGYVXTXX SXXXRSXXXX XXXXXXXXXX XGGXXXXXXX XXXXXXXXFX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttttgtttaa ctttnagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt gggacttcga aacagacccc gaataccagg cgaagctgga ctgggtccaa gagttcatgg tcaacgaact cgaaccactg gatctggtag ctctcgatcc ctatgacaag aagaaccccg agatgatggc cgtccttcgg ccgttgcagc aggtgaagaa ccaggggctc tgggccgctc acctgcgacc ggaagtcggc gggcaggggt ttggccaagt caagcttgcg ctgctgaacg aaatcctcgg acgctcgcgc tgggcgccct cggtgttcgg ttgtcaggca ccagactcgg gtaacgccga gatcctggcg ctgttcggca ccgaggacca gaaggcccgc tacctgcaac cgctgctgga cggcgaaatc acctcctgct actcgatgac agaacctcan ggaggttcgg atccagggtc gttcgtcacg gccgccaccc gcgacggana cagctggatc atcaacgggg agaagtggtt ttccaccaac gccaagcacg cctcgtnctt catcgtgatg gccgtgacca acccgcaggc ccgcanctac nanaagatgt cgctgttcat cgtccctgcn ganacacccn gtatcgagat cgtccgcaac gtcgnngtcn ggnccnantc nagcaannnn nncannnncg gntacgtnnc tacacnangt tcngnngnnn gncgatcatg nngntngnng nnaagnnnnn ntntgancnn nnnnnnncnn ntcggnggnn gtcnnnttnn ncnnnnnnnt nnnnnntnnn tnnnnncnan nnnntttgnn nnnnnnnnnn nnnnnncnnn nnnnnnnnna nnngnnnntt nnantnnnnn nnnngnnann nnnncnnnng ntnnnnnnnn nnnnnnnnnn nnnnnnncnn ngnngnnnna nnnaancnnn nnntnnnnnn nnnnnnnnnn nnnnnngncn nngnnnnnng nnnnnnngnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna nnnnnnnnnn nnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVWDFETDP EYQAKLDWVQ EFMVNELEPL DLVALDPYDK KNPEMMAVLR PLQQVKNQGL WAAHLRPEVG GQGFGQVKLA LLNEILGRSR WAPSVFGCQA PDSGNAEILA LFGTEDQKAR YLQPLLDGEI TSCYSMTEPQ GGSDPGSFVT AATRDGDSWI INGEKWFSTN AKHASFFIVM AVTNPQARTY EKMSLFIVPA ETPGIEIVRN VGVGTESSKH ASHGYVRYHD VRVPADHVLG GEGQAFMTAQ TRLGGGRIHH AMRTIALARR AFDMMCERAV SRQTRHGRLS DLQMTQEKVA DSWIQIEQFR LLVLRTAWLI DKHHDYQKVR RDIAAVKVAM PQVLHDVVQR AMHLHGALGV SDEMPFVKMM VAAESLGIAD GATELHKMTV ARRTLRQYEP VATPFPSQHI PTRRAAAEAR LAERLEHSIA EF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtgggac ttcgaaacag accccgaata ccaggcgaag ctggactggg tccaagagtt catggtcaac gaactcgaac cactggatct ggtagctctc gatccctatg acaagaagaa ccccgagatg atggccgtcc ttcggccgtt gcagcaggtg aagaaccagg ggctctgggc cgctcacctg cgaccggaag tcggcgggca ggggtttggc caagtcaagc ttgcgctgct gaacgaaatc ctcggacgct cgcgctgggc gccctcggtg ttcggttgtc aggcaccaga ctcgggtaac gccgagatcc tggcgctgtt cggcaccgag gaccagaagg cccgctacct gcaaccgctg ctggacggcg aaatcacctc ctgctactcg atgacagaac ctcagggagg ttcggatcca gggtcgttcg tcacggccgc cacccgcgac ggagacagct ggatcatcaa cggggagaag tggttttcca ccaacgccaa gcacgcctcg ttcttcatcg tgatggccgt gaccaacccg caggcccgca cctacgagaa gatgtcgctg ttcatcgtcc ctgcggagac acccggtatc gagatcgtcc gcaacgtcgg cgtcgggacc gagtcgagca agcacgccag ccacggctac gtccgctacc acgatgttcg ggtgccggcc gatcatgtgc tcggcggtga aggccaggcg ttcatgaccg cgcagacgcg actcggcggc ggtcgcattc accacgcgat gcgcacaatt gcattggcac gacgagcctt tgacatgatg tgcgaacgcg ccgtatccag gcagaccaga cacggccggc tttccgactt gcagatgacg caggagaagg tcgccgacag ctggatccaa atcgagcagt tccggctact ggtgctgcgc acggcgtggc tgatcgacaa gcaccacgac tatcagaagg tgcgtcgcga catcgcggcg gtgaaggtcg caatgccaca ggtgctgcac gacgtggtgc agcgcgccat gcacctgcac ggcgcgctcg gcgtttccga cgagatgccg ttcgtcaaga tgatggtggc cgccgaatcg ctgggcatcg ccgacggcgc caccgaactg cacaagatga cggtggcacg ccggacactg cgccaatacg agcctgttgc aacgccgttc ccgtcacagc acatacccac ccggcgagcg gcggccgaag cgcggctggc cgagcgcctg gaacacagca tcgcggagtt caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa