SSGCID: Target Page

MyulA.00309.a

Acyl-CoA dehydrogenase FadE1_1

CENTER ID:	MyulA.00309.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00309.a.A1.GE29676	Full length( MyulA.00309.a )		1	435

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.5083
RefSeq:	YP_907854.1
UniProt:	A0PVL4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPWDFSTEPE FEKKLDWIRG FVREEVEPLE VVFPGCEFLP LNDERRRIVD PLKQRVRDEG LWAPHLGPEL GGQGFGAVKL TLINEILGRS PWAPIVFGTQ APDTGNAEII ARFGTPDQKD RYLQGLLSGE IFSCFSMTEP QGGADPRVFT TRAVRDGDGW VISGRKYFSS NASVPSFFIV VAITNPDVAV HHGASTFLVP AGTKGLTIEA NHHLVGALPH EPGHSLMHYD DVRVGADALL GEPGQGFLIL QTRLAGGRLH HAMRSIGVAQ RAVEMMSRRA KSRFTQGSSL ADKQLVQAFI ADSYTELMPF RLAVLHAAWM IDTAGEHAAR AEIGACKILA SQVLKSIGLR AIQVHGALGT TDQLPLVSVL LGGVALGLAD GPTEAHKVNL ARMLLKNYEP EDPQWPSESL AVRQAAARAK YGQLVDRVPA LPDSP

NT Sequence

ATGCCTTGGG ACTTCTCCAC CGAACCGGAG TTCGAGAAGA AGCTCGACTG GATCCGCGGG TTCGTGCGCG AGGAGGTGGA ACCGCTCGAG GTGGTGTTCC CCGGCTGCGA GTTCCTGCCG CTCAACGATG AACGCCGCCG CATCGTGGAT CCCCTCAAAC AACGAGTCCG CGACGAAGGC TTGTGGGCCC CACATTTGGG TCCGGAACTA GGCGGGCAGG GCTTTGGCGC GGTGAAACTG ACCCTGATCA ACGAGATCCT GGGACGCAGC CCCTGGGCTC CGATCGTGTT CGGCACCCAG GCACCCGACA CCGGCAACGC GGAGATCATT GCGCGGTTTG GCACCCCGGA TCAAAAGGAC CGCTACCTGC AGGGCTTGCT GTCCGGCGAG ATCTTCTCCT GCTTCTCCAT GACCGAGCCG CAGGGCGGTG CCGATCCGCG CGTCTTCACC ACCCGCGCCG TCCGCGACGG TGACGGTTGG GTGATCAGCG GCCGCAAGTA TTTCTCGTCC AACGCCTCGG TCCCATCGTT TTTCATCGTC GTCGCGATCA CCAATCCGGA TGTGGCCGTT CACCACGGAG CGTCGACGTT CCTGGTCCCG GCTGGAACCA AGGGCTTGAC CATCGAAGCC AACCACCACC TGGTCGGCGC CCTGCCCCAC GAACCGGGTC ACTCCCTGAT GCACTACGAC GACGTTCGAG TCGGTGCGGA TGCGCTGCTT GGGGAACCGG GGCAGGGATT TCTGATCCTG CAAACCCGCC TGGCCGGTGG CCGGCTGCAT CACGCGATGC GCTCCATCGG GGTGGCGCAG CGCGCGGTCG AGATGATGTC ACGTCGAGCG AAAAGTCGAT TCACCCAGGG CAGTTCGCTG GCCGATAAGC AGTTGGTGCA AGCGTTCATC GCCGACTCCT ACACCGAGCT GATGCCGTTC CGGCTGGCGG TGCTGCACGC GGCCTGGATG ATCGACACCG CCGGCGAGCA CGCGGCGCGC GCCGAGATCG GCGCCTGCAA GATCCTGGCA TCCCAAGTGC TCAAATCGAT TGGTCTGCGT GCCATCCAGG TGCACGGCGC GCTGGGCACC ACCGATCAGC TACCGCTGGT CAGCGTGCTA CTCGGCGGTG TTGCGCTGGG GCTGGCAGAC GGCCCCACCG AAGCCCACAA GGTGAACCTG GCGCGGATGC TGCTGAAGAA CTACGAGCCC GAAGACCCGC AGTGGCCCAG CGAATCACTG GCCGTCCGGC AAGCGGCCGC CCGCGCCAAG TACGGCCAAC TCGTCGACCG TGTTCCGGCC CTTCCCGATT CACCG

Details for MyulA.00309.a.A1.GE29676

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	49kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	49

Validated AA Sequence

Validated NT Sequence

antnnnnnnn nnnattttgt ttaactttaa gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg ccttgggact tctccaccga accggagttc gagaagaagc tcgactggat ccgcgggttc gtgcgcgagg aggtggaacc gctcgaggtg gtgttccccg gctgcgagtt cctgccgctc aacgatgaac gccgccgcat cgtggatccc ctcaaacaac gagtccgcga cgaaggcttg tgggccccac atttgggtcc ggaactaggc gggcagggct ttggcgcggt gaaactgacc ctgatcaacg agatcctggg acgcagcccc tgggctccga tcgtgttcgg cacccaggca cccgacaccg gcaacgcgga gatcattgcg cggtttggca ccccggatca aaaggaccgc tacctgcagg gcttgctgtc cggcgagatc ttctcctgct tctccatgac cganccgcan ggcggtgccg atccgcgcgt cttcaccacc cgcgccgtcc gcgacggtga cggttgggtg atcagcggcc gcaagtattt ctcgtccaac gcctcggncc catcgttttt catcgtcgtc gcgatcacca atccngatgt ngncgttcan cncggancgt cnacgttcct ggtcccggct ggnncaaggg cttgancatc gaanccnacc ccacctggtc gnnnnccngn cccncnannn ggncnnnncc ngatgnncta cnacnacntt cnannngnnn ngntgncctg ntnnnnnnng gnnnnnnttt nancngnnan nccgnctnnn nnnnnntgnn tnnnnnnann nnntccntnn nnngnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaannc nntnnncnna nttnnntngn cnnnnnnnnn nngnnnannn tnnnnncnnn nnnnnnnncn nnnnnnnnnn ggnnnnnnnc nnncngnnnn nnnnanccnn nnnnannnnn nnnnnnnaan nnnnnnnnnn nnnnnnnnng gnnnnnngnn cnnnnnnnnn nnnnnnnnnn c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPWDFSTEP EFEKKLDWIR GFVREEVEPL EVVFPGCEFL PLNDERRRIV DPLKQRVRDE GLWAPHLGPE LGGQGFGAVK LTLINEILGR SPWAPIVFGT QAPDTGNAEI IARFGTPDQK DRYLQGLLSG EIFSCFSMTE PQGGADPRVF TTRAVRDGDG WVISGRKYFS SNASVPSFFI VVAITNPDVA VHHGASTFLV PAGTKGLTIE ANHHLVGALP HEPGHSLMHY DDVRVGADAL LGEPGQGFLI LQTRLAGGRL HHAMRSIGVA QRAVEMMSRR AKSRFTQGSS LADKQLVQAF IADSYTELMP FRLAVLHAAW MIDTAGEHAA RAEIGACKIL ASQVLKSIGL RAIQVHGALG TTDQLPLVSV LLGGVALGLA DGPTEAHKVN LARMLLKNYE PEDPQWPSES LAVRQAAARA KYGQLVDRVP ALPDSP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccttgggac ttctccaccg aaccggagtt cgagaagaag ctcgactgga tccgcgggtt cgtgcgcgag gaggtggaac cgctcgaggt ggtgttcccc ggctgcgagt tcctgccgct caacgatgaa cgccgccgca tcgtggatcc cctcaaacaa cgagtccgcg acgaaggctt gtgggcccca catttgggtc cggaactagg cgggcagggc tttggcgcgg tgaaactgac cctgatcaac gagatcctgg gacgcagccc ctgggctccg atcgtgttcg gcacccaggc acccgacacc ggcaacgcgg agatcattgc gcggtttggc accccggatc aaaaggaccg ctacctgcag ggcttgctgt ccggcgagat cttctcctgc ttctccatga ccgagccgca gggcggtgcc gatccgcgcg tcttcaccac ccgcgccgtc cgcgacggtg acggttgggt gatcagcggc cgcaagtatt tctcgtccaa cgcctcggtc ccatcgtttt tcatcgtcgt cgcgatcacc aatccggatg tggccgttca ccacggagcg tcgacgttcc tggtcccggc tggaaccaag ggcttgacca tcgaagccaa ccaccacctg gtcggcgccc tgccccacga accgggtcac tccctgatgc actacgacga cgttcgagtc ggtgcggatg cgctgcttgg ggaaccgggg cagggatttc tgatcctgca aacccgcctg gccggtggcc ggctgcatca cgcgatgcgc tccatcgggg tggcgcagcg cgcggtcgag atgatgtcac gtcgagcgaa aagtcgattc acccagggca gttcgctggc cgataagcag ttggtgcaag cgttcatcgc cgactcctac accgagctga tgccgttccg gctggcggtg ctgcacgcgg cctggatgat cgacaccgcc ggcgagcacg cggcgcgcgc cgagatcggc gcctgcaaga tcctggcatc ccaagtgctc aaatcgattg gtctgcgtgc catccaggtg cacggcgcgc tgggcaccac cgatcagcta ccgctggtca gcgtgctact cggcggtgtt gcgctggggc tggcagacgg ccccaccgaa gcccacaagg tgaacctggc gcggatgctg ctgaagaact acgagcccga agacccgcag tggcccagcg aatcactggc cgtccggcaa gcggccgccc gcgccaagta cggccaactc gtcgaccgtg ttccggccct tcccgattca ccgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa