SSGCID: Target Page

MyulA.00305.b

Enoyl-CoA hydratase, EchA12

CENTER ID:	MyulA.00305.b
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00305.b.A1.GE29674	Full length( MyulA.00305.b )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.1783
RefSeq:	YP_905473.1
UniProt:	A0PNT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLVLLEHPR PDIALVTLNR PERMNSIAFD VMVPLKEVLE EVSYDNSVRV VVLTGAGRGF SSGADHKSAG AVPNVGGLTR PTYALRSMEL LDEVILMLRR LHQPVIAAVN GPAIGGGLCL ALAADVRVAA ASAYFRAAGI NNGLTASELG LSYLLPRAIG SSRAFEIMLT GRDVTAEEAE RIGLVSCRVP DGQLLDTCYA ITARMAAFSR PGIELTKRTL WSGLDAASLE GHMQAEGLGQ LFVRLLTANF EEAVAARAEK RPAVFTDDK

NT Sequence

GTGTCTTTGG TCCTGTTAGA GCACCCGCGT CCAGACATTG CGCTGGTCAC CCTCAACCGG CCCGAGCGGA TGAACTCCAT CGCCTTCGAC GTCATGGTGC CGCTCAAGGA GGTGCTCGAG GAGGTCAGCT ACGACAACTC GGTGCGGGTG GTGGTGCTGA CCGGGGCTGG CCGGGGCTTC TCGTCTGGCG CTGATCACAA GTCAGCGGGC GCCGTTCCGA ACGTTGGGGG ATTGACCCGG CCGACATACG CGCTGCGTTC GATGGAGCTG CTCGACGAAG TGATCCTGAT GCTGCGGCGT TTGCATCAGC CGGTGATCGC CGCGGTCAAC GGGCCGGCCA TCGGTGGGGG ACTATGCCTG GCGTTGGCCG CCGATGTCCG GGTGGCCGCG GCCAGCGCGT ACTTCCGGGC CGCCGGCATC AACAATGGGC TGACCGCCAG CGAGCTGGGC CTGAGTTACC TGTTGCCCAG GGCCATCGGG TCCTCGCGCG CCTTCGAGAT CATGTTGACC GGCCGCGATG TCACCGCGGA GGAAGCCGAG CGGATTGGTC TGGTGTCCTG CCGGGTGCCC GATGGTCAGC TACTGGACAC CTGTTACGCG ATCACGGCAC GAATGGCGGC CTTCTCCAGG CCGGGAATTG AGTTGACCAA ACGCACACTG TGGAGTGGAC TAGACGCCGC TAGCCTGGAG GGGCACATGC AGGCCGAGGG GCTGGGCCAG CTCTTCGTCC GCCTGCTGAC CGCAAACTTC GAAGAAGCGG TGGCCGCACG CGCCGAGAAA CGGCCGGCGG TTTTCACCGA TGACAAG

Details for MyulA.00305.b.A1.GE29674

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	31kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSLVLLEH PRPDIALVTL NRPERMNSIA FDVMVPLKEV LEEVSYDNSV RVVVLTGAGR GFSSGADHKS AGAVPNVGGL TRPTYALRSM ELLDEVILML RRLHQPVIAA VNGPAIGGGL CLALAADVRV AAASAYFRAA GINNGLTASE LGLSYLLPRA IGSSRAFEIM LTGRDVTAEE AERIGLVSCR VPDGQLLDTC YAITARMAAF SRPGIELTKR TLWSXLDAAS LEGHMQAEGL GQLFVRLLTA NFXEXVAARA EKXAGGFHR

Validated NT Sequence

ttttgtttnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgtc tttggtcctg ttagagcacc cgcgtccaga cattgcgctg gtcaccctca accggcccga gcggatgaac tccatcgcct tcgacgtcat ggtgccgctc aaggaggtgc tcgaggaggt cagctacgac aactcggtgc gggtggtggt gctgaccggg gctggccggg gcttctcgtc tggcgctgat cacaagtcag cgggcgccgt tccgaacgtt gggggattga cccggccgac atacgcgctg cgttcgatgg agctgctcga cgaagtgatc ctgatgctgc ggcgtttgca tcagccggtg atcgccgcgg tcaacgggcc ggccatcggt gggggactat gcctggcgtt ggccgccgat gtccgggtgg ccgcggccag cgcgtacttc cgggccgccg gcatcaacaa tgggctgacc gccagcgagc tgggcctgag ttacctgttg cccagggcca tcgggtcctc gcgcgccttc gagatcatgt tgaccggccg cgatgtcacc gcggaggaag ccgagcggat tggtctggtg tcctgccggg tgcccgatgg tcagctactg gacacctgtt acgcgatcac ggcacgaatg gcggccttct ccaggccggg aattgagttg accaaacgca cactgtggag tgnactagac gccgctagcc tggaggggca catgcaggcc gaggggctgg gccagctctt cgtccgcctg ctgaccgcaa acttcnaaga ancggtggcc gcacgcgccg agaaacnggc cggcggtttt caccgatgac aagtaataac agcacgaaca agttctgcag ccaagcttct cgaggnnncg gctgctaacn aagcccgaaa ggaagctgan tngntgntgc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtctttg gtcctgttag agcacccgcg tccagacatt gcgctggtca ccctcaaccg gcccgagcgg atgaactcca tcgccttcga cgtcatggtg ccgctcaagg aggtgctcga ggaggtcagc tacgacaact cggtgcgggt ggtggtgctg accggggctg gccggggctt ctcgtctggc gctgatcaca agtcagcggg cgccgttccg aacgttgggg gattgacccg gccgacatac gcgctgcgtt cgatggagct gctcgacgaa gtgatcctga tgctgcggcg tttgcatcag ccggtgatcg ccgcggtcaa cgggccggcc atcggtgggg gactatgcct ggcgttggcc gccgatgtcc gggtggccgc ggccagcgcg tacttccggg ccgccggcat caacaatggg ctgaccgcca gcgagctggg cctgagttac ctgttgccca gggccatcgg gtcctcgcgc gccttcgaga tcatgttgac cggccgcgat gtcaccgcgg aggaagccga gcggattggt ctggtgtcct gccgggtgcc cgatggtcag ctactggaca cctgttacgc gatcacggca cgaatggcgg ccttctccag gccgggaatt gagttgacca aacgcacact gtggagtgga ctagacgccg ctagcctgga ggggcacatg caggccgagg ggctgggcca gctcttcgtc cgcctgctga ccgcaaactt cgaagaagcg gtggccgcac gcgccgagaa acggccggcg gttttcaccg atgacaagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa