SSGCID: Target Page

MyulA.00305.a

Enoyl-CoA hydratase, EchA12_2

CENTER ID:	MyulA.00305.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00305.a.A1.GE29673	Full length( MyulA.00305.a )		1	263

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.5134
RefSeq:	YP_907885.1
UniProt:	A0PVP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQTLQHNIAC VTLNRPTRLN AIDGSLLDAM DDALDALSGG EFRAAILTGA GRGFCAGADL SGTGQPWTKA TPTTPPFKAT YDAQVRLADQ LTRLYELPIP VIAAVNGVAV GGGLAFALHC DIRIAAEDAR FGSVFIKAGF SSMDMGTSYL LPRIVGAGVA RELMLTGRII DAVEAYRIRL VHEVVVADEL LTAAQKLAGS IAENNAYGVW QTKIGLNAAP DAPSLRHAKE IENRTEIISG FTNNPAEAAR AHQEKRPPAW DSL

NT Sequence

TTGCAAACTC TTCAGCACAA CATTGCTTGC GTCACCTTGA ACCGGCCGAC CCGGTTGAAC GCCATCGACG GATCGCTGCT CGACGCGATG GACGACGCCC TCGACGCGCT GAGCGGGGGG GAGTTTCGGG CCGCGATCCT GACCGGCGCC GGGCGCGGGT TTTGTGCCGG AGCGGATTTG AGCGGCACCG GACAGCCTTG GACGAAGGCC ACACCCACCA CCCCGCCGTT CAAGGCCACC TACGATGCCC AGGTCAGACT GGCCGACCAA TTGACCCGGC TCTACGAGCT GCCCATTCCC GTGATCGCGG CGGTCAACGG CGTCGCGGTC GGTGGCGGGC TGGCCTTTGC GCTGCATTGC GATATCCGGA TCGCTGCCGA AGACGCCAGG TTCGGATCGG TGTTCATCAA GGCCGGGTTC TCGTCGATGG ACATGGGCAC CAGTTATCTG CTGCCCAGAA TCGTCGGTGC CGGGGTGGCG CGCGAACTCA TGCTGACCGG CCGCATCATC GACGCTGTGG AGGCCTATCG GATCAGGCTG GTGCACGAGG TGGTGGTAGC GGACGAATTG TTGACTGCGG CGCAGAAGCT GGCCGGATCG ATCGCCGAGA ACAATGCGTA TGGCGTGTGG CAGACCAAGA TTGGGCTCAA CGCGGCGCCG GATGCCCCCA GCCTGCGGCA TGCCAAGGAA ATCGAGAACC GTACCGAGAT TATCAGCGGC TTCACCAACA ACCCGGCCGA GGCCGCCCGC GCGCATCAGG AGAAACGTCC GCCGGCATGG GATTCGCTG

Details for MyulA.00305.a.A1.GE29673

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	105

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLQTLQHNI ACVTLNRPTR LNAIDGSLLD AMDDALDALS GGEFRAAILT GAGRGFCAGA DLSGTGQPWT KATPTTPPFK ATYDAQVRLA DQLTRLYELP IPVIAAVNGV AVGGGLAFAL HCDIRIAAED ARFGSVFIKA GFSSMDMGTS YLLPRIVGAG VARELMLTGR IIDAVEAYRI RLVHEVVVAD ELLTAAQKLA GSIAENNAYG VWQTKIGLNA APDAPSLRHA KEIENRTEII SGFTNNPAEX ARAHXRNVRR HGIRCNKQHE QVLQPAXRGS GC

Validated NT Sequence

attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgttg caaactcttc agcacaacat tgcttgcgtc accttgaacc ggccgacccg gttgaacgcc atcgacggat cgctgctcga cgcgatggac gacgccctcg acgcgctgag cgggggggag tttcgggccg cgatcctgac cggcgccggg cgcgggtttt gtgccggagc ggatttgagc ggcaccggac agccttggac gaaggccaca cccaccaccc cgccgttcaa ggccacctac gatgcccagg tcagactggc cgaccaattg acccggctct acgagctgcc cattcccgtg atcgcggcgg tcaacggcgt cgcggtcggt ggcgggctgg cctttgcgct gcattgcgat atccggatcg ctgccgaaga cgccaggttc ggatcggtgt tcatcaaggc cgggttctcg tcgatggaca tgggcaccag ttatctgctg cccagaatcg tcggtgccgg ggtggcgcgc gaactcatgc tgaccggccg catcatcgac gctgtggagg cctatcggat caggctggtg cacgaggtgg tggtagcgga cgaattgttg actgcggcgc agaagctggc cggatcgatc gccgagaaca atgcgtatgg cgtgtggcag accaagattg ggctcaacgc ggcgccggat gcccccagcc tgcggcatgc caaggaaatc gagaaccgta ccgagattat cagcggcttc accaacaacc cggccgaggn cgcccgcgcg catcngagaa acgtccgccg gcatgggatt cgctgtaata aacagcacga acaagttctg cagccagctn ctcgaggatc cggctgctaa nnaagcccga aangaagctg anttngctgc tgcnnnnnnn nnnnnannan aaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcaaact cttcagcaca acattgcttg cgtcaccttg aaccggccga cccggttgaa cgccatcgac ggatcgctgc tcgacgcgat ggacgacgcc ctcgacgcgc tgagcggggg ggagtttcgg gccgcgatcc tgaccggcgc cgggcgcggg ttttgtgccg gagcggattt gagcggcacc ggacagcctt ggacgaaggc cacacccacc accccgccgt tcaaggccac ctacgatgcc caggtcagac tggccgacca attgacccgg ctctacgagc tgcccattcc cgtgatcgcg gcggtcaacg gcgtcgcggt cggtggcggg ctggcctttg cgctgcattg cgatatccgg atcgctgccg aagacgccag gttcggatcg gtgttcatca aggccgggtt ctcgtcgatg gacatgggca ccagttatct gctgcccaga atcgtcggtg ccggggtggc gcgcgaactc atgctgaccg gccgcatcat cgacgctgtg gaggcctatc ggatcaggct ggtgcacgag gtggtggtag cggacgaatt gttgactgcg gcgcagaagc tggccggatc gatcgccgag aacaatgcgt atggcgtgtg gcagaccaag attgggctca acgcggcgcc ggatgccccc agcctgcggc atgccaagga aatcgagaac cgtaccgaga ttatcagcgg cttcaccaac aacccggccg aggccgcccg cgcgcatcag gagaaacgtc cgccggcatg ggattcgctg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa