MyulA.00277.b

Lipase/esterase LipN

CENTER ID: MyulA.00277.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00277.b.A1.GE29670 Full length( MyulA.00277.b ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.2360
RefSeq: YP_905903.1
UniProt: A0PQ13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNSLPGETD LHPETVHATM SWLSRVQNTV TVVGAKVIPW VPDVAKRLIT RGRSVIIDGN TLDPALQLML SGMRVVGLDG LVIDDDLAAS RAHMREAMLG FPGPQIHVDV EELTLPGPAG DISARHYRPP GDAAAPLLVF YHGGGWALGD LDTHDAVCRL TCRDAGIHVL AIDYRLSPEH RAPAAIDDAF AAFEWAHAHA AELGALPGRV AVGGDSAGGN LAAVVSQLAR DSGGPAPIFQ WLIYPRTDFA GRTRSASLFA RGFLLTKRDI DWFHSQYLKG SGIEPTDPRV SPLRAESLAG LAPALIAVAG FDPLRDEGEN YATALRAAGT PVDLRAMGSL THGFLNLFPL GGGCAAATSE LISALRAHLT RV
NT Sequence
ATGACAAATA GCCTGCCAGG TGAGACGGAC CTCCATCCCG AAACGGTGCA CGCGACGATG AGCTGGCTGA GTCGCGTGCA GAACACCGTC ACCGTCGTCG GCGCCAAGGT CATCCCATGG GTTCCCGATG TCGCCAAACG GCTGATCACA CGGGGTCGCT CGGTCATCAT CGACGGAAAC ACCCTCGATC CCGCATTGCA GCTGATGTTG TCGGGCATGC GGGTCGTGGG TCTGGACGGG CTGGTGATCG ATGACGACCT GGCCGCTTCC CGCGCCCACA TGCGCGAGGC GATGTTGGGA TTTCCCGGTC CGCAGATCCA TGTCGATGTC GAGGAATTAA CCCTGCCCGG ACCTGCCGGC GACATCAGCG CCAGGCATTA CCGTCCGCCC GGCGATGCGG CCGCGCCGTT GCTGGTCTTC TACCACGGGG GCGGGTGGGC ACTCGGCGAC CTCGACACCC ACGACGCGGT CTGCCGACTG ACTTGTCGCG ATGCGGGCAT CCATGTCCTG GCGATCGACT ACCGGCTGTC GCCCGAGCAT CGCGCCCCCG CCGCGATCGA CGATGCGTTC GCGGCCTTTG AGTGGGCGCA CGCGCACGCC GCCGAGCTTG GTGCTCTGCC GGGACGGGTT GCGGTCGGTG GCGACAGTGC GGGGGGCAAC CTGGCGGCCG TGGTGAGCCA GCTGGCCCGA GACAGCGGAG GCCCGGCCCC GATTTTCCAG TGGCTCATCT ACCCACGAAC CGACTTTGCC GGGCGGACCC GTTCCGCGAG CCTGTTCGCC CGAGGGTTCT TGCTGACCAA ACGCGACATC GACTGGTTTC ACTCCCAGTA CCTGAAGGGC TCCGGCATCG AGCCCACGGA CCCGCGGGTA TCGCCGTTAC GAGCCGAATC ACTGGCCGGG CTGGCGCCGG CGCTGATCGC GGTGGCAGGC TTCGACCCGT TGCGTGACGA AGGCGAGAAC TACGCGACCG CCCTGCGGGC CGCCGGCACC CCGGTTGACC TGCGCGCCAT GGGGTCGCTG ACGCACGGGT TCCTCAATCT GTTTCCGCTC GGTGGTGGTT GTGCCGCCGC AACCAGCGAG CTGATTTCTG CGCTGCGTGC ACACCTGACC CGGGTT
Details for MyulA.00277.b.A1.GE29670
HARVESTED ON: 6/22/2010
SEQUENCED ON: 12/5/2014
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMTNSLPG ETDLHPETVH ATMSWLSRVQ NTVTVVGAKV IPWVPDVAKR LITRGRSVII DGNTLDPALQ LMLSGMRVVG LDGLVIDDDL AASRAHMREA MLGFPGPQIH VDVEELTLPG PAGDISARHY RPPGDAAAPL LVFYHGGGWA LGDLDTHDAV CRLTCRDAGI HVLAIDYRLS PEHRAPAAID DAFAAFEWAH AHAAELGALP GRVAVGGDSA GGNLAAVVSQ LARDSGGPAP IFQWLIYPRT DFAGRTRSAS LFARGFLLTK RDIDWFHSQY LKGSGIEPTD PRVSPLRAES LAGLAPALIA VAGFDPLRDE GENYATALRA AGTPVDLRAM GSLTHGFLNL FPLGGGCAAA TSELISALRA HLTRV
Validated NT Sequence
ttattaaacc cgggtcaggt gtgcacgcag cgcagaaatc agctcgctgg ttgcggcggc acaaccacca ccgagcggaa acagattgag gaacccgtgc gtcagcgacc ccatggcgcg caggtcaacc ggggtgccgg cggcccgcag ggcggtcgcg tagttctcgc cttcgtcacg caacgggtcg aagcctgcca ccgcgatcag cgccggcgcc agcccggcca gtgattcggc tcgtaacggc gatacccgcg ggtccgtggg ctcgatgccg gagcccttca ggtactggga gtgaaaccag tcgatgtcgc gtttggtcag caagaaccct cgggcgaaca ggctcgcgga acgggtccgc ccggcaaagt cggttcgtgg gtagatgagc cactggaaaa tcggggccgg gcctccgctg tctcgggcca gctggctcac cacggccgcc aggttgcccc ccgcactgtc gccaccgacc gcaacccgtc ccggcagagc accaagctcg gcggcgtgcg cgtgcgccca ctcaaaggcc gcgaacgcat cgtcgatcgc ggcgggggcg cgatgctcgg gcgacagccg gtagtcgatc gccaggacat ggatgcccgc atcgcgacaa gtcagtcggc agaccgcgtc gtgggtgtcg aggtcgccga gtgcccaccc gcccccgtgg tagaagacca gcaacggcgc ggccgcatcg ccgggcggac ggtaatgcct ggcgctgatg tcgccggcag gtccgggcag ggttaattcc tcgacatcga catggatctg cggaccggga aatcccaaca tcgcctcgcg catgtgggcg cgggaagcgg ccaggtcgtc atcgatcacc agcccgtcca gacccacgac ccgcatgccc gacaacatca gctgcaatgc gggatcgagg gtgtttccgt cgatgatgac cgagcgaccc cgtgtgatca gccgtttggc gacatcggga acccatggga tgaccttggc gccgacgacg gtgacggtgt tctgcacgcg actcagccag ctcatcgtcg cgtgcaccgt ttcgggatgg aggtccgtct cacctggcag gctatttgtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSLPGET DLHPETVHAT MSWLSRVQNT VTVVGAKVIP WVPDVAKRLI TRGRSVIIDG NTLDPALQLM LSGMRVVGLD GLVIDDDLAA SRAHMREAML GFPGPQIHVD VEELTLPGPA GDISARHYRP PGDAAAPLLV FYHGGGWALG DLDTHDAVCR LTCRDAGIHV LAIDYRLSPE HRAPAAIDDA FAAFEWAHAH AAELGALPGR VAVGGDSAGG NLAAVVSQLA RDSGGPAPIF QWLIYPRTDF AGRTRSASLF ARGFLLTKRD IDWFHSQYLK GSGIEPTDPR VSPLRAESLA GLAPALIAVA GFDPLRDEGE NYATALRAAG TPVDLRAMGS LTHGFLNLFP LGGGCAAATS ELISALRAHL TRV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaatagc ctgccaggtg agacggacct ccatcccgaa acggtgcacg cgacgatgag ctggctgagt cgcgtgcaga acaccgtcac cgtcgtcggc gccaaggtca tcccatgggt tcccgatgtc gccaaacggc tgatcacacg gggtcgctcg gtcatcatcg acggaaacac cctcgatccc gcattgcagc tgatgttgtc gggcatgcgg gtcgtgggtc tggacgggct ggtgatcgat gacgacctgg ccgcttcccg cgcccacatg cgcgaggcga tgttgggatt tcccggtccg cagatccatg tcgatgtcga ggaattaacc ctgcccggac ctgccggcga catcagcgcc aggcattacc gtccgcccgg cgatgcggcc gcgccgttgc tggtcttcta ccacgggggc gggtgggcac tcggcgacct cgacacccac gacgcggtct gccgactgac ttgtcgcgat gcgggcatcc atgtcctggc gatcgactac cggctgtcgc ccgagcatcg cgcccccgcc gcgatcgacg atgcgttcgc ggcctttgag tgggcgcacg cgcacgccgc cgagcttggt gctctgccgg gacgggttgc ggtcggtggc gacagtgcgg ggggcaacct ggcggccgtg gtgagccagc tggcccgaga cagcggaggc ccggccccga ttttccagtg gctcatctac ccacgaaccg actttgccgg gcggacccgt tccgcgagcc tgttcgcccg agggttcttg ctgaccaaac gcgacatcga ctggtttcac tcccagtacc tgaagggctc cggcatcgag cccacggacc cgcgggtatc gccgttacga gccgaatcac tggccgggct ggcgccggcg ctgatcgcgg tggcaggctt cgacccgttg cgtgacgaag gcgagaacta cgcgaccgcc ctgcgggccg ccggcacccc ggttgacctg cgcgccatgg ggtcgctgac gcacgggttc ctcaatctgt ttccgctcgg tggtggttgt gccgccgcaa ccagcgagct gatttctgcg ctgcgtgcac acctgacccg ggttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa