SSGCID: Target Page

MyulA.00168.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD)

CENTER ID:	MyulA.00168.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00168.a.A1.GE29657	Full length( MyulA.00168.a )		1	227

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.4832
RefSeq:	YP_907669.1
UniProt:	A0PV29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGGTQGVAA IVPAAGSGQR LAAGVPKAFY QLEGQTLVER AVRGLFESGV VDTVVVAVPP DRTDEAKLIL GREATIVAGG ADRTESVSRA LSALSAGVSP EFVLVHDAAR ALTPPALVVR LVDMLRAGHA AVVPVLPLTD TIKAVDANGV VLGTPERAGL RAVQTPQGFS TELLLRAYRH AGVSAFTDDA SLVEHVGGQV HVVGGDPLAF KITNRLDLLL AHAVVRG

NT Sequence

GTGGCCGGGG GAACCCAGGG CGTCGCCGCG ATTGTTCCGG CCGCAGGGTC GGGTCAGCGG CTCGCGGCTG GCGTGCCTAA GGCGTTCTAT CAACTCGAAG GTCAAACTCT GGTTGAGCGA GCCGTCAGGG GCTTGTTCGA ATCCGGGGTG GTGGACACCG TCGTGGTGGC GGTACCCCCA GATCGCACCG ACGAGGCAAA GCTGATCCTG GGCCGCGAGG CCACCATTGT GGCCGGCGGC GCCGATCGCA CCGAAAGTGT GAGCCGGGCC TTGTCCGCCT TGTCTGCCGG CGTCTCGCCG GAATTCGTAC TTGTCCATGA CGCTGCGCGG GCGCTGACGC CGCCGGCACT GGTCGTGCGG TTGGTCGACA TGTTGCGGGC TGGCCATGCC GCGGTCGTAC CGGTGCTGCC GCTCACTGAC ACAATCAAGG CTGTGGATGC CAATGGGGTG GTTCTGGGTA CGCCGGAACG CGCGGGGCTG AGGGCGGTGC AGACCCCGCA GGGGTTCTCC ACCGAACTGC TGCTGCGCGC CTACCGACAC GCGGGCGTCT CCGCCTTCAC CGATGACGCA TCGCTGGTTG AGCATGTCGG TGGGCAGGTG CACGTGGTTG GCGGCGACCC GCTGGCGTTC AAGATCACCA ACCGTCTCGA TCTGTTGCTG GCCCACGCGG TTGTGCGCGG G

Details for MyulA.00168.a.A1.GE29657

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVAGGTQGV AAIVPAAGSG QRLAAGVPKA FYQLEGQTLV ERAVRGLFES GVVDTVVVAV PPDRTDEAKL ILGREATIVA GGADRTESVS RALSALSAGV SPEFVLVHDA ARALTPPALV VRLVDMLRAG HAAVVPVLPL TDTIKAVDAN GVVLGTPERA GLRAVQTPQG FSTELLLRAY RHAGVSAFTD DASLVEHVGG QVHVVGGDPL AFKITNRLDL LLAHAVVRG

Validated NT Sequence

ttattacccg cgcacaaccg cgtgggccag caacagatcg agacggttgg tgatcttgaa cgccagcggg tcgccgccaa ccacgtgcac ctgcccaccg acatgctcaa ccagcgatgc gtcatcggtg aaggcggaga cgcccgcgtg tcggtaggcg cgcagcagca gttcggtgga gaacccctgc ggggtctgca ccgccctcag ccccgcgcgt tccggcgtac ccagaaccac cccattggca tccacagcct tgattgtgtc agtgagcggc agcaccggta cgaccgcggc atggccagcc cgcaacatgt cgaccaaccg cacgaccagt gccggcggcg tcagcgcccg cgcagcgtca tggacaagta cgaattccgg cgagacgccg gcagacaagg cggacaaggc ccggctcaca ctttcggtgc gatcggcgcc gccggccaca atggtggcct cgcggcccag gatcagcttt gcctcgtcgg tgcgatctgg gggtaccgcc accacgacgg tgtccaccac cccggattcg aacaagcccc tgacggctcg ctcaaccaga gtttgacctt cgagttgata gaacgcctta ggcacgccag ccgcgagccg ctgacccgac cctgcggccg gaacaatcgc ggcgacgccc tgggttcccc cggccaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccggg ggaacccagg gcgtcgccgc gattgttccg gccgcagggt cgggtcagcg gctcgcggct ggcgtgccta aggcgttcta tcaactcgaa ggtcaaactc tggttgagcg agccgtcagg ggcttgttcg aatccggggt ggtggacacc gtcgtggtgg cggtaccccc agatcgcacc gacgaggcaa agctgatcct gggccgcgag gccaccattg tggccggcgg cgccgatcgc accgaaagtg tgagccgggc cttgtccgcc ttgtctgccg gcgtctcgcc ggaattcgta cttgtccatg acgctgcgcg ggcgctgacg ccgccggcac tggtcgtgcg gttggtcgac atgttgcggg ctggccatgc cgcggtcgta ccggtgctgc cgctcactga cacaatcaag gctgtggatg ccaatggggt ggttctgggt acgccggaac gcgcggggct gagggcggtg cagaccccgc aggggttctc caccgaactg ctgctgcgcg cctaccgaca cgcgggcgtc tccgccttca ccgatgacgc atcgctggtt gagcatgtcg gtgggcaggt gcacgtggtt ggcggcgacc cgctggcgtt caagatcacc aaccgtctcg atctgttgct ggcccacgcg gttgtgcgcg ggaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa