SSGCID: Target Page

MyulA.00148.a

Integrase/recombinase, XerD

CENTER ID:	MyulA.00148.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00148.a.A1.GE29653	Full length( MyulA.00148.a )		1	313

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.2031
RefSeq:	YP_905650.1
UniProt:	A0PPB0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTLTLDTQL QGYLDHLAIE RGVAANTLSS YRRDLRRYSK HLEDRGITDL AKVGEDDVSE FLVALRRGDP ESGVLGLSAV SAARALIAVR GLHRFAAAEG LAALDVARAV RPPTPGRRLP KSLTIDEVLA LLEGAGGDNP ADGPLTLRNR ALLELLYSTG SRISEAVGLD VDDIDTQART VLLQGKGGKQ RLVPVGRPAV QALDAYLVRG RPELARRGRG TPAIFLNARG GRLSRQSAWQ VLQDAAERAD ITSGVSPHML RHSFATHLLE GGADVRVVQE LLGHASVTTT QIYTLVTVHA LREVWAGAHP RAT

NT Sequence

GTGAGCACCC TGACCCTGGA CACGCAGTTG CAGGGTTATC TCGACCATTT AGCGATCGAA CGTGGCGTTG CCGCCAACAC GTTGAGCTCC TACCGGCGGG ACCTGCGCCG CTACTCCAAG CACCTCGAAG ATCGGGGGAT CACCGATTTA GCCAAGGTGG GCGAGGACGA TGTGAGCGAG TTCCTGGTCG CCCTGCGACG GGGAGATCCC GAATCCGGAG TGCTCGGGCT GTCGGCGGTG TCGGCGGCCC GAGCGCTGAT TGCGGTGCGC GGGTTGCATC GGTTCGCGGC CGCGGAGGGA CTGGCCGCGC TGGATGTGGC GCGCGCCGTG CGTCCGCCCA CACCGGGCCG AAGGCTGCCC AAGAGTCTGA CCATCGATGA GGTGCTGGCC CTGCTCGAAG GTGCCGGTGG CGACAATCCG GCGGACGGCC CGCTGACGCT GCGCAACCGG GCATTGCTGG AGCTGTTGTA TTCCACCGGG TCTCGGATCT CCGAGGCGGT CGGACTCGAT GTCGACGATA TCGATACGCA GGCCAGGACG GTGTTGTTAC AGGGCAAGGG CGGTAAGCAG CGGCTGGTGC CGGTGGGGCG TCCCGCGGTA CAAGCCTTGG ACGCCTATCT GGTGCGCGGA CGTCCGGAGC TCGCCCGCCG CGGCCGCGGC ACCCCGGCGA TTTTCCTCAA CGCGCGCGGC GGTCGCCTGT CGCGCCAAAG CGCTTGGCAG GTGTTGCAGG ATGCGGCCGA ACGTGCCGAC ATCACCTCGG GGGTATCGCC GCACATGCTG CGCCATTCGT TTGCGACTCA TCTGCTGGAA GGCGGCGCGG ATGTCCGGGT GGTGCAGGAG CTTCTGGGTC ATGCCTCGGT GACAACGACG CAGATCTACA CGCTGGTCAC CGTGCACGCG CTGCGCGAAG TGTGGGCGGG CGCGCACCCC CGGGCCACC

Details for MyulA.00148.a.A1.GE29653

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	78
PERCENT COVERAGE:	104

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSTLTLDT QLQGYLDHLA IERGVAANTL SSYRRDLRRY SKHLEDRGIT DLAKVGEDDV SEFLVALRRG DPESGVLGLS AVSAARALIA VRGLHRFAAA EGLAALDVAR AVRPPTPGRR LPKSLTIDEV LALLEGAGGD NPADGPLTLR NRALLELLYS TGSRISEAVG LDVDDIDTQA RTVLLQGKGG KQRLVPVGRP AVQALDAYLV RGRPELARRG RGTPAIFLNA RGGRLSRQSA WXXCRMXPNV PTSPRGYRRT CCAIRXXLIC WXXRXVXXXL WXMXXXXTXX XXLXXXXXXX XXXXXXXXXX XXXXXXXXAX XXXXXXXXXX

Validated NT Sequence

tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgag caccctgacc ctggacacgc agttgcaggg ttatctcgac catttagcga tcgaacgtgg cgttgccgcc aacacgttga gctcctaccg gcgggacctg cgccgctact ccaagcacct cgaagatcgg gggatcaccg atttagccaa ggtgggcgag gacgatgtga gcgagttcct ggtcgccctg cgacggggag atcccgaatc cggagtgctc gggctgtcgg cggtgtcggc ggcccgagcg ctgattgcgg tgcgcgggtt gcatcggttc gcggccgcgg agggactggc cgcgctggat gtggcgcgcg ccgtgcgtcc gcccacaccg ggccgaaggc tgcccaagag tctgaccatc gatgaggtgc tggccctgct cgaaggtgcc ggtggcgaca atccggcgga cggcccgctg acgctgcgca accgggcatt gctggagctg ttgtattcca ccgggtctcg gatctccgag gcggtcggac tcgatgtcga cgatatcgat acgcaggcca ggacggtgtt gttacagggc aagggcggta agcagcggct ggtgccggtg gggcgtcccg cggtacaagc cttggacgcc tatctggtgc gcggacgtcc ggagctcgcc cgccgcggcc gcggcacccc ggcgattttc ctcaacgcgc gcggcggtcg cctgtcgcgc caaagcgctt ggnngngttg caggatgcnn ccnaacgtgc cgacatcacc tcgggggtat cgccgcacat gctgcgccat tcgttngcna ctcatctgct ggnnnnngcg nnnngtccng gnggngctnt gggncatgcn tngnganann acgnnnannn ncncnctnnn cnnnnngnan nnnntgnnnn aannnnngnn nnnnnnnncc cncngnnnnc nnntaannnn nnnnannnnn ncngcancnn nnnnnnnnnn ancnnnngnn nnnaannnna ng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSTLTLDT QLQGYLDHLA IERGVAANTL SSYRRDLRRY SKHLEDRGIT DLAKVGEDDV SEFLVALRRG DPESGVLGLS AVSAARALIA VRGLHRFAAA EGLAALDVAR AVRPPTPGRR LPKSLTIDEV LALLEGAGGD NPADGPLTLR NRALLELLYS TGSRISEAVG LDVDDIDTQA RTVLLQGKGG KQRLVPVGRP AVQALDAYLV RGRPELARRG RGTPAIFLNA RGGRLSRQSA WQVLQDAAER ADITSGVSPH MLRHSFATHL LEGGADVRVV QELLGHASVT TTQIYTLVTV HALREVWAGA HPRAT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcacc ctgaccctgg acacgcagtt gcagggttat ctcgaccatt tagcgatcga acgtggcgtt gccgccaaca cgttgagctc ctaccggcgg gacctgcgcc gctactccaa gcacctcgaa gatcggggga tcaccgattt agccaaggtg ggcgaggacg atgtgagcga gttcctggtc gccctgcgac ggggagatcc cgaatccgga gtgctcgggc tgtcggcggt gtcggcggcc cgagcgctga ttgcggtgcg cgggttgcat cggttcgcgg ccgcggaggg actggccgcg ctggatgtgg cgcgcgccgt gcgtccgccc acaccgggcc gaaggctgcc caagagtctg accatcgatg aggtgctggc cctgctcgaa ggtgccggtg gcgacaatcc ggcggacggc ccgctgacgc tgcgcaaccg ggcattgctg gagctgttgt attccaccgg gtctcggatc tccgaggcgg tcggactcga tgtcgacgat atcgatacgc aggccaggac ggtgttgtta cagggcaagg gcggtaagca gcggctggtg ccggtggggc gtcccgcggt acaagccttg gacgcctatc tggtgcgcgg acgtccggag ctcgcccgcc gcggccgcgg caccccggcg attttcctca acgcgcgcgg cggtcgcctg tcgcgccaaa gcgcttggca ggtgttgcag gatgcggccg aacgtgccga catcacctcg ggggtatcgc cgcacatgct gcgccattcg tttgcgactc atctgctgga aggcggcgcg gatgtccggg tggtgcagga gcttctgggt catgcctcgg tgacaacgac gcagatctac acgctggtca ccgtgcacgc gctgcgcgaa gtgtgggcgg gcgcgcaccc ccgggccacc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa