MyulA.00113.a

Polyketide synthase MbtC

CENTER ID: MyulA.00113.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00113.a.A1.GE29651 Full length( MyulA.00113.a ) 1 439
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4241
RefSeq: YP_907241.1
UniProt: A0PTV1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTNGSAGAA DPIVISGMAV EAPGGIDSPA SLWAALAESR ELLSPFPRDR GWPVDDLLSV SDLEGWGQVC DSGGFLDGAT TFDPGFFGIS AREAIVLHPQ QRVAMRVAWK ALENSGINPG STAGAEGGCF VGMSPMEYGP RASVADAYSG HRIASLGQLG GAARISHSLG LIGPSISVDT ACASSLSALH LAATAVREDE CEWALAGAVC VMGSPGAFYE FARLNALSED GHCRAYSDNA TGTVWGEGAG MVLVERESRA QREGRRIYGR IAAIRTNHNG KGKPILVPRV RAQEQVIRKT LEVAGIDPVE VGMIEGHGTA TPAGDPVELI ALFKTYGAAG SEALLGSIKS NAGHSQAASG ILALIKLLLA GQHGQIPPTL FADNPTKTVD WDLTGLRLAT KLHEWEPKHG VRYGAVSSFG AGGANAHAII SMPATGVGQ
NT Sequence
ATGAGCACCA ACGGATCCGC CGGAGCAGCC GACCCGATCG TCATCTCCGG AATGGCCGTC GAGGCACCCG GCGGAATCGA CAGCCCGGCT TCTTTGTGGG CAGCGCTTGC CGAATCCAGG GAACTACTGA GTCCCTTTCC CCGCGACCGC GGCTGGCCCG TCGATGATCT GTTGTCGGTA TCCGACCTGG AAGGTTGGGG GCAGGTTTGC GATTCCGGCG GATTCCTGGA TGGAGCAACC ACATTCGACC CTGGCTTCTT CGGTATCAGC GCCCGCGAGG CCATCGTGTT GCATCCCCAG CAACGGGTTG CGATGCGAGT CGCGTGGAAG GCACTGGAGA ATTCCGGAAT CAACCCCGGC TCGACTGCCG GGGCAGAGGG CGGCTGTTTC GTCGGCATGT CGCCAATGGA GTACGGACCA CGGGCATCGG TAGCCGATGC CTACAGCGGT CATCGGATCG CCTCGCTGGG CCAACTGGGG GGCGCCGCCC GAATTTCGCA CAGTCTTGGC CTGATCGGTC CTTCGATCAG TGTCGACACG GCGTGCGCCT CGTCATTGTC CGCACTGCAC CTGGCCGCCA CGGCGGTGCG GGAGGACGAG TGCGAATGGG CGCTTGCCGG CGCCGTGTGC GTGATGGGTT CGCCCGGAGC GTTTTACGAG TTCGCGAGAC TCAATGCCCT TTCCGAGGAC GGGCATTGCC GCGCCTATTC CGATAACGCC ACCGGCACCG TGTGGGGGGA AGGCGCGGGA ATGGTTCTCG TCGAGCGGGA ATCACGAGCA CAGCGAGAGG GCCGGCGAAT CTACGGACGT ATCGCGGCCA TCCGCACCAA CCACAACGGT AAAGGCAAGC CAATCTTGGT TCCCCGGGTA CGAGCGCAAG AGCAGGTTAT TCGCAAGACG CTGGAGGTGG CGGGAATTGA TCCGGTCGAG GTCGGAATGA TCGAAGGACA CGGCACTGCA ACGCCTGCCG GCGACCCAGT CGAATTGATC GCGCTGTTCA AGACCTACGG CGCTGCGGGG TCGGAAGCTC TGCTCGGGTC GATCAAGTCC AATGCCGGAC ACTCGCAGGC GGCATCCGGA ATCCTGGCGT TGATCAAGCT GCTGCTGGCC GGCCAACACG GTCAGATTCC GCCAACCCTT TTCGCGGACA ACCCAACCAA AACGGTGGAC TGGGATCTGA CCGGCCTGCG GCTGGCGACG AAACTACATG AGTGGGAGCC CAAACACGGT GTTCGCTACG GTGCCGTGTC TTCGTTCGGT GCGGGCGGCG CCAATGCCCA TGCCATCATT TCGATGCCGG CTACCGGAGT CGGACAG
Details for MyulA.00113.a.A1.GE29651
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 47kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTNGSAGA ADPIVISGMA VXAPGGIXSP ASLXAALAES XXLLXPFPRX RXXXVDDLLS VSDLEGWGQV CDSGGFLDGA TTFDPGFFGI SAREAIVLHP QQRVAMRVAW KALENSGINP GSTAGAEGGC FVGMSPMEYG PRASVADAYS GHRIASLGQL GGAARISHSL GLIGPSISVD TACASSLSAL HLAATAVRED ECEWALAGAV CVMGSPGAFY EFARLNALSE DGHCRAYSDN ATGTVWGEGA GMVLVERXSX XQREGXRIYX RIAAIRXNHN GXXKPIXXPR GTXARXXXSX XXXXGGNXSX XXXSXXXXLX XXXXPXXXSX XXXXXXLXGX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgagc accaacggat ccgccggagc agccgacccg atcgtcatct ccggaatggc cgtcnaggca cccggcggaa tcnacagccc ggcttctttg ngggcagcgc ttgccgaatc cnngnaacta ctgantccct ttccccgcna ccgcgnnagn nccgtcgatg atctgttgtc ggtatccgac ctggaaggtt gggggcaggt ttgcgattcc ggcggattcc tggatggagc aaccacattc gaccctggct tcttcggtat cagcgcccgc gaggccatcg tgttgcatcc ccagcaacgg gttgcgatgc gagtcgcgtg gaaggcactg gagaattccg gaatcaaccc cggctcgact gccggggcag agggcggctg tttcgtcggc atgtcgccaa tggagtacgg accacgggca tcggtagccg atgcctacag cggtcatcgg atcgcctcgc tgggccaact ggggggcgcc gcccgaattt cgcacagtct tggcctgatc ggtccttcga tcagtgtcga cacggcgtgc gcctcgtcat tgtccgcact gcacctggcc gccacggcgg tgcgggagga cgagtgcgaa tgggcgcttg ccggcgccgt gtgcgtgatg ggttcgcccg gagcgtttta cgagttcgcg agactcaatg ccctttccga ggacgggcat tgccgcgcct attccgataa cgccaccggc accgtgtggg gggaaggngc gggaatggtt ctcgtcgagc ggnaatcncn ancacagcga gagggcnnnc gaatctacng acgtatcgcg gccatccgnn ncaaccacaa cggtaanngc aagccaatcn tgnnnccccg gggtacnanc gcaagancag nttantcnna nangcnngna gnnggcggga atngatccnn tcnangannn tcnaannnnn nnnnnctgca nnncnnnnnc ganccnnncn antngtcgng ntnnntnnnn nnnncgnnnc tnnngggnnn nnnncnnnnn nnngnnnatn annnnnnnnn nnnnnnncnn gnnnnnnngn nnnnnnnnna nnannnnnnn nnnnnnnnnn nnnnnnnann nnnnnnnntt nnnnnnnnan cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTNGSAGA ADPIVISGMA VEAPGGIDSP ASLWAALAES RELLSPFPRD RGWPVDDLLS VSDLEGWGQV CDSGGFLDGA TTFDPGFFGI SAREAIVLHP QQRVAMRVAW KALENSGINP GSTAGAEGGC FVGMSPMEYG PRASVADAYS GHRIASLGQL GGAARISHSL GLIGPSISVD TACASSLSAL HLAATAVRED ECEWALAGAV CVMGSPGAFY EFARLNALSE DGHCRAYSDN ATGTVWGEGA GMVLVERESR AQREGRRIYG RIAAIRTNHN GKGKPILVPR VRAQEQVIRK TLEVAGIDPV EVGMIEGHGT ATPAGDPVEL IALFKTYGAA GSEALLGSIK SNAGHSQAAS GILALIKLLL AGQHGQIPPT LFADNPTKTV DWDLTGLRLA TKLHEWEPKH GVRYGAVSSF GAGGANAHAI ISMPATGVGQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaccaac ggatccgccg gagcagccga cccgatcgtc atctccggaa tggccgtcga ggcacccggc ggaatcgaca gcccggcttc tttgtgggca gcgcttgccg aatccaggga actactgagt ccctttcccc gcgaccgcgg ctggcccgtc gatgatctgt tgtcggtatc cgacctggaa ggttgggggc aggtttgcga ttccggcgga ttcctggatg gagcaaccac attcgaccct ggcttcttcg gtatcagcgc ccgcgaggcc atcgtgttgc atccccagca acgggttgcg atgcgagtcg cgtggaaggc actggagaat tccggaatca accccggctc gactgccggg gcagagggcg gctgtttcgt cggcatgtcg ccaatggagt acggaccacg ggcatcggta gccgatgcct acagcggtca tcggatcgcc tcgctgggcc aactgggggg cgccgcccga atttcgcaca gtcttggcct gatcggtcct tcgatcagtg tcgacacggc gtgcgcctcg tcattgtccg cactgcacct ggccgccacg gcggtgcggg aggacgagtg cgaatgggcg cttgccggcg ccgtgtgcgt gatgggttcg cccggagcgt tttacgagtt cgcgagactc aatgcccttt ccgaggacgg gcattgccgc gcctattccg ataacgccac cggcaccgtg tggggggaag gcgcgggaat ggttctcgtc gagcgggaat cacgagcaca gcgagagggc cggcgaatct acggacgtat cgcggccatc cgcaccaacc acaacggtaa aggcaagcca atcttggttc cccgggtacg agcgcaagag caggttattc gcaagacgct ggaggtggcg ggaattgatc cggtcgaggt cggaatgatc gaaggacacg gcactgcaac gcctgccggc gacccagtcg aattgatcgc gctgttcaag acctacggcg ctgcggggtc ggaagctctg ctcgggtcga tcaagtccaa tgccggacac tcgcaggcgg catccggaat cctggcgttg atcaagctgc tgctggccgg ccaacacggt cagattccgc caaccctttt cgcggacaac ccaaccaaaa cggtggactg ggatctgacc ggcctgcggc tggcgacgaa actacatgag tgggagccca aacacggtgt tcgctacggt gccgtgtctt cgttcggtgc gggcggcgcc aatgcccatg ccatcatttc gatgccggct accggagtcg gacagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa