MytuD.20412.a

Probable fatty acid synthase/synthetase Fas (Rv2524c)

CENTER ID: MytuD.20412.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.20412.a.B2.GE43838 FAS MaoC, active-site containing 1211 1330
MytuD.20412.a.B3.GE43841 FAS MaoC/FabD 1211 1722
MytuD.20412.a.MB2.GE43840 FAS MaoC, active-site containing 1211 1330
MytuD.20412.a.MB3.GE43842 FAS MaoC/FabD 1211 1722
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.111.peg.2822
TubercuList: Rv2524c
MTB Network Portal: Rv2524c
UniProt: P95029

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIHEHDRVS ADRGGDSPHT THALVDRLMA GEPYAVAFGG QGSAWLETLE ELVSATGIET ELATLVGEAE LLLDPVTDEL IVVRPIGFEP LQWVRALAAE DPVPSDKHLT SAAVSVPGVL LTQIAATRAL ARQGMDLVAT PPVAMAGHSQ GVLAVEALKA GGARDVELFA LAQLIGAAGT LVARRRGISV LGDRPPMVSV TNADPERIGR LLDEFAQDVR TVLPPVLSIR NGRRAVVITG TPEQLSRFEL YCRQISEKEE ADRKNKVRGG DVFSPVFEPV QVEVGFHTPR LSDGIDIVAG WAEKAGLDVA LARELADAIL IRKVDWVDEI TRVHAAGARW ILDLGPGDIL TRLTAPVIRG LGIGIVPAAT RGGQRNLFTV GATPEVARAW SSYAPTVVRL PDGRVKLSTK FTRLTGRSPI LLAGMTPTTV DAKIVAAAAN AGHWAELAGG GQVTEEIFGN RIEQMAGLLE PGRTYQFNAL FLDPYLWKLQ VGGKRLVQKA RQSGAAIDGV VISAGIPDLD EAVELIDELG DIGISHVVFK PGTIEQIRSV IRIATEVPTK PVIMHVEGGR AGGHHSWEDL DDLLLATYSE LRSRANITVC VGGGIGTPRR AAEYLSGRWA QAYGFPLMPI DGILVGTAAM ATKESTTSPS VKRMLVDTQG TDQWISAGKA QGGMASSRSQ LGADIHEIDN SASRCGRLLD EVAGDAEAVA ERRDEIIAAM AKTAKPYFGD VADMTYLQWL RRYVELAIGE GNSTADTASV GSPWLADTWR DRFEQMLQRA EARLHPQDFG PIQTLFTDAG LLDNPQQAIA ALLARYPDAE TVQLHPADVP FFVTLCKTLG KPVNFVPVID QDVRRWWRSD SLWQAHDARY DADAVCIIPG TASVAGITRM DEPVGELLDR FEQAAIDEVL GAGVEPKDVA SRRLGRADVA GPLAVVLDAP DVRWAGRTVT NPVHRIADPA EWQVHDGPEN PRATHSSTGA RLQTHGDDVA LSVPVSGTWV DIRFTLPANT VDGGTPVIAT EDATSAMRTV LAIAAGVDSP EFLPAVANGT ATLTVDWHPE RVADHTGVTA TFGEPLAPSL TNVPDALVGP CWPAVFAAIG SAVTDTGEPV VEGLLSLVHL DHAARVVGQL PTVPAQLTVT ATAANATDTD MGRVVPVSVV VTGADGAVIA TLEERFAILG RTGSAELADP ARAGGAVSAN ATDTPRRRRR DVTITAPVDM RPFAVVSGDH NPIHTDRAAA LLAGLESPIV HGMWLSAAAQ HAVTATDGQA RPPARLVGWT ARFLGMVRPG DEVDFRVERV GIDQGAEIVD VAARVGSDLV MSASARLAAP KTVYAFPGQG IQHKGMGMEV RARSKAARKV WDTADKFTRD TLGFSVLHVV RDNPTSIIAS GVHYHHPDGV LYLTQFTQVA MATVAAAQVA EMREQGAFVE GAIACGHSVG EYTALACVTG IYQLEALLEM VFHRGSKMHD IVPRDELGRS NYRLAAIRPS QIDLDDADVP AFVAGIAEST GEFLEIVNFN LRGSQYAIAG TVRGLEALEA EVERRRELTG GRRSFILVPG IDVPFHSRVL RVGVAEFRRS LDRVMPRDAD PDLIIGRYIP NLVPRLFTLD RDFIQEIRDL VPAEPLDEIL ADYDTWLRER PREMARTVFI ELLAWQFASP VRWIETQDLL FIEEAAGGLG VERFVEIGVK SSPTVAGLAT NTLKLPEYAH STVEVLNAER DAAVLFATDT DPEPEPEEDE PVAESPAPDV VSEAAPVAPA ASSAGPRPDD LVFDAADATL ALIALSAKMR IDQIEELDSI ESITDGASSR RNQLLVDLGS ELNLGAIDGA AESDLAGLRS QVTKLARTYK PYGPVLSDAI NDQLRTVLGP SGKRPGAIAE RVKKTWELGE GWAKHVTVEV ALGTREGSSV RGGAMGHLHE GALADAASVD KVIDAAVASV AARQGVSVAL PSAGSGGGAT IDAAALSEFT DQITGREGVL ASAARLVLGQ LGLDDPVNAL PAAPDSELID LVTAELGADW PRLVAPVFDP KKAVVFDDRW ASAREDLVKL WLTDEGDIDA DWPRLAERFE GAGHVVATQA TWWQGKSLAA GRQIHASLYG RIAAGAENPE PGRYGGEVAV VTGASKGSIA ASVVARLLDG GATVIATTSK LDEERLAFYR TLYRDHARYG AALWLVAANM ASYSDVDALV EWIGTEQTES LGPQSIHIKD AQTPTLLFPF AAPRVVGDLS EAGSRAEMEM KVLLWAVQRL IGGLSTIGAE RDIASRLHVV LPGSPNRGMF GGDGAYGEAK SALDAVVSRW HAESSWAARV SLAHALIGWT RGTGLMGHND AIVAAVEEAG VTTYSTDEMA ALLLDLCDAE SKVAAARSPI KADLTGGLAE ANLDMAELAA KAREQMSAAA AVDEDAEAPG AIAALPSPPR GFTPAPPPQW DDLDVDPADL VVIVGGAEIG PYGSSRTRFE MEVENELSAA GVLELAWTTG LIRWEDDPQP GWYDTESGEM VDESELVQRY HDAVVQRVGI REFVDDGAID PDHASPLLVS VFLEKDFAFV VSSEADARAF VEFDPEHTVI RPVPDSTDWQ VIRKAGTEIR VPRKTKLSRV VGGQIPTGFD PTVWGISADM AGSIDRLAVW NMVATVDAFL SSGFSPAEVM RYVHPSLVAN TQGTGMGGGT SMQTMYHGNL LGRNKPNDIF QEVLPNIIAA HVVQSYVGSY GAMIHPVAAC ATAAVSVEEG VDKIRLGKAQ LVVAGGLDDL TLEGIIGFGD MAATADTSMM CGRGIHDSKF SRPNDRRRLG FVEAQGGGTI LLARGDLALR MGLPVLAVVA FAQSFGDGVH TSIPAPGLGA LGAGRGGKDS PLARALAKLG VAADDVAVIS KHDTSTLAND PNETELHERL ADALGRSEGA PLFVVSQKSL TGHAKGGAAV FQMMGLCQIL RDGVIPPNRS LDCVDDELAG SAHFVWVRDT LRLGGKFPLK AGMLTSLGFG HVSGLVALVH PQAFIASLDP AQRADYQRRA DARLLAGQRR LASAIAGGAP MYQRPGDRRF DHHAPERPQE ASMLLNPAAR LGDGEAYIG
NT Sequence
gtgacgatcc acgagcacga ccgggtgtcc gctgatcgcg gcggggacag cccgcatacc acccacgctc tggtcgatcg cctcatggct ggtgagccct acgctgtcgc attcggtggc cagggcagcg cctggctgga aaccctcgaa gagctggtgt cggccaccgg gatagaaacc gagttggcga cgttggtcgg tgaggcagag ctgttgctcg atccggtcac cgacgagctg attgtggtgc gcccgatcgg tttcgagccg ctgcaatggg tacgcgcact ggcggccgag gacccggttc cgtccgacaa gcacctgacg tcggccgccg tgtcggtgcc cggcgtgttg cttacccaga tcgcggcgac ccgggcgctg gcccgtcaag gcatggacct cgtggccacc ccgccggtcg ccatggcggg gcattcgcaa ggtgtgctgg cggtggaagc cctcaaggct ggtggggcac gcgacgtcga gctgtttgcc ttggcccagt tgatcggtgc cgccggaacg ctggtggccc gccggcgcgg aatttccgtc ctgggcgatc gcccgccgat ggtatcggtc accaacgccg accccgagcg catcggccgg ttgctcgacg agttcgccca ggacgtgcgc acggtgctgc caccggtgtt gtccatccgc aacggccggc gtgccgtcgt catcaccggc acccccgagc agctgtcgcg tttcgagctt tattgccgcc agatctccga gaaggaagaa gccgaccgca agaacaaggt ccgcggcggc gacgtcttct cgccggtctt cgagccggtg caggtggagg tgggctttca caccccgcgg ctatccgacg ggatcgacat cgtcgcgggc tgggccgaga aggcgggcct cgatgtcgcc ttggctcggg agctggccga tgccatcttg atcagaaagg tcgactgggt cgacgagatc acccgtgtcc acgcggccgg cgcccgctgg atcctcgacc tggggccggg cgacatcctg acccgactga ccgcaccggt gatccgcggc ctgggcatcg gcatcgtgcc ggcggctacc cgcggtggcc agcgcaacct gttcaccgtc ggcgccaccc ccgaggttgc ccgggcctgg tcgagctacg caccgaccgt ggttcgcctc cccgacggca gggtcaagct ctcgacgaag ttcacccggc tgaccggccg ctcgccgatc ctgctcgcgg gcatgacccc gaccaccgtg gacgccaaga tcgtcgccgc ggcggccaac gccgggcact gggccgagct ggccggcggc gggcaggtca ccgaagagat cttcggtaac cgcatcgaac aaatggccgg cctgctcgag ccgggccgca cctatcagtt caacgcgctg ttcctcgatc cctacctgtg gaagcttcag gtgggcggca agcggttggt gcagaaggcc cgccagtccg gcgccgcgat cgacggcgtg gtgatcagcg ccggcatccc agacctcgac gaggccgtcg agctgatcga cgaactgggc gacatcggca tcagccacgt cgtgttcaaa cccgggacca tcgagcagat ccgctcggtg attcgcatcg ccaccgaggt gcccaccaag ccggtgatca tgcacgtcga gggcgggcgc gccggcgggc accattcctg ggaggatctc gacgacctgc tgctggctac ctactcggag ttgcgctcac gcgccaacat cacggtgtgc gtcggcggcg gcattggcac cccgagaagg gctgcggaat atttgtccgg gcgctgggcg caggcctacg gcttcccatt gatgccgatc gacggcatcc tggtcggcac cgcggcgatg gccaccaagg aatccaccac gtcgccatcg gtcaagcgga tgctcgtcga cactcagggc accgaccaat ggatcagcgc cggaaaagcg cagggcggca tggcctccag ccgcagtcag ctcggtgccg atatccacga gatcgacaac agcgcatccc ggtgcgggcg gctgctcgac gaggtggccg gtgacgcgga ggcggtcgcg gagcgtcgcg acgagatcat cgcggcgatg gccaagaccg ccaagcccta cttcggcgac gtcgccgaca tgacctacct gcagtggctg cggcgctacg tcgaactggc catcggggaa ggcaactcga ccgccgacac cgcctcggtg ggcagcccgt ggctggccga cacctggcgg gaccgcttcg agcagatgct gcagcgtgcc gaagcccggt tgcacccaca ggatttcggc ccgatccaga cgctattcac cgatgctggc ctgctggaca atccgcagca ggcgatcgcc gccctgctgg cgcgctaccc cgacgccgag accgtgcagt tgcatcccgc ggatgtgccc tttttcgtga cgttgtgcaa gacgctgggc aagccggtca acttcgtgcc ggtgatcgac caggacgtgc ggcgctggtg gcgcagcgac tcgctgtggc aggcccacga cgcccgctac gacgccgatg cggtgtgcat cattccgggc accgcgtcgg tagccggcat cacccggatg gatgaacccg tcggtgagtt gctggaccgt ttcgagcaag ccgcaatcga tgaagtgctc ggcgccggtg tcgagccgaa ggatgtcgcg tcgcgccggc tgggccgcgc cgacgtggcc ggaccgttgg ctgtcgtcct cgacgcaccc gatgtgcgct gggccggtcg caccgtgacc aacccggtgc atcggatcgc cgacccggcc gaatggcagg tgcacgatgg acccgaaaac ccgcgcgcca cacactcatc caccggcgcc cggctgcaga cgcacggcga cgacgtcgcc ttgagcgtgc ccgtctcggg cacctgggtc gacatccgat tcacgttgcc ggccaacacc gtcgatggcg gcaccccggt gatcgccacc gaggacgcca ccagcgccat gcgcacggtg ctggcgatcg ccgccggtgt cgacagcccg gagttcttgc ctgcggtggc caacgggacg gccactttga cggtggactg gcaccccgag cgtgttgccg accacaccgg cgtcaccgcc acgttcggtg agccgctggc acccagcctc accaacgtgc ccgacgcgct cgtcggccct tgttggccag cggttttcgc ggccatcgga tcggcggtca ccgacaccgg tgagccggtg gtggaaggcc tgctgagcct ggtgcatctg gaccacgccg cccgcgtggt cggtcagctg cccacggtcc cggcccaatt gaccgtcacc gcaacggctg ccaacgcaac cgatacggac atgggccgcg tcgtgccggt ctcggtcgtc gttaccggcg ccgatggcgc cgtgatcgcc actctcgagg agcgattcgc gatcctgggt cgcaccggtt ccgccgagct cgccgacccg gcgcgagccg gtggcgcggt gtcggcgaac gccaccgaca ccccgcgccg tcgccgccgc gacgtcacga tcaccgcgcc ggtcgacatg cgcccgttcg cggtggtgtc cggcgaccac aaccccattc acaccgaccg ggccgccgcg ctgcttgccg gcctggagtc gccgatcgtg cacggcatgt ggctgtcggc cgcggcgcaa cacgcggtga ccgccaccga cgggcaggcc cggccaccgg cccggctggt cggctggacc gcgcggtttt tgggcatggt gcgccccggc gacgaggtgg acttccgcgt cgagcgcgtc ggaatcgacc agggcgcaga gattgtggac gtggccgcgc gcgtcgggtc ggatctagtg atgtcggcct ccgcgcgact ggccgcaccc aagacggtct acgcattccc cggccagggc atccaacaca agggcatggg catggaggtg cgcgcccgct ccaaggcggc ccgcaaggtg tgggacaccg cggacaagtt cacccgcgac accctgggct tctcggtact gcacgtggtc cgcgacaacc cgaccagcat catcgccagc ggtgtgcact accaccaccc cgacggggtg ctctacctga cgcagttcac ccaggtcgcg atggcgacgg tggcggccgc gcaggtcgcc gagatgcgtg aacagggagc cttcgtcgaa ggcgccatcg cgtgcggcca ctcggtcggc gagtacaccg cgctggcctg cgtgaccggc atctaccaac tggaagcctt gctggagatg gtgtttcacc gcgggtcgaa gatgcacgac atcgttccgc gcgacgagct cggccgctcc aactatcggc tggcggccat ccggccgtcc cagatcgacc tcgacgacgc cgacgtgccc gcgttcgtcg ccgggatcgc ggagagcacc ggtgaattcc tggagatcgt gaatttcaac ctgcgtggct cgcaatacgc gatcgcgggc acggtacgcg gcctcgaggc gctcgaggcc gaggtggagc ggcgccgcga gctcaccggc ggccgacggt cgttcatttt ggtgcccggc atcgatgttc cgttccactc gcgagtgctg cgggtcgggg tggccgaatt ccggcgctcg ctggaccggg tcatgccgcg cgacgcggac cccgacctga tcatcgggcg ctacattccc aacctggtgc cgcggttgtt caccctggac cgcgacttca tccaggaaat ccgggatttg gtgcccgccg agccgctcga cgagatcctc gccgactacg acacctggct tcgcgagcgt ccgcgcgaga tggcgcgcac ggtgttcatc gagctgctgg catggcaatt cgccagcccg gtgcgctgga tcgagacgca ggatctgctg ttcatcgagg aggccgccgg cgggctgggt gtggagcgat tcgtcgagat cggtgtgaag agctcaccga cggtggcggg tcttgccacc aacaccctca aactgcccga atacgcccac agcacagtgg aagtgctcaa cgccgagcgt gatgccgcgg tgctgttcgc caccgacacc gacccggagc cggagccgga ggaagacgag ccggtcgcgg aatcgcccgc gccggacgtc gtctcggaag ccgcccccgt cgcgccggcc gcttcgtcgg cgggcccgcg tcccgacgat ctggttttcg acgccgccga tgccacgctg gcgctgatcg cgctctcggc caagatgcgc atcgaccaga tcgaagaact cgactccatc gagtccatca ccgacggtgc gtcgtcgcgg cgcaaccagc tgctggtgga cctgggctcc gagctgaacc tcggtgccat tgacggcgcc gccgaatcgg acctggccgg tctgcgctca caggtgacca aactggcgcg cacctacaag ccttacggcc cagtgctttc cgacgccatc aacgaccagc ttcgcaccgt cctcggaccg tcgggcaagc ggcccggcgc catcgccgag cgggtgaaga agacctggga gctcggtgag ggctgggcca agcatgtcac cgtcgaggtc gcgctgggca cccgcgaggg cagcagcgtt cgcggcggcg ccatgggcca cctgcacgag ggcgcgctgg ccgatgccgc ctccgtcgac aaggtcatcg acgcggcggt cgcatcggtg gccgcgcgcc agggcgtttc ggtagcgctg ccgtcggccg gtagtggtgg cggcgccacc atcgacgcgg ccgcgctcag cgagttcacc gaccaaatca ccggccgtga gggcgtgctg gcctccgcgg cccgcctggt gctggggcag ctgggactgg acgaccccgt caacgccttg ccggccgccc ccgattccga gctgatcgac ttggtcaccg ccgaactggg agcggactgg ccgcggttgg tggcaccggt gttcgacccc aagaaggccg tcgtattcga cgaccgctgg gccagcgccc gcgaggacct ggtgaagctg tggctgaccg acgagggcga catcgacgcc gactggccgc gcctggcgga gcgcttcgag ggtgccggcc acgtcgtggc gacccaggct acctggtggc aaggtaagtc gctggccgcg ggccggcaga tccatgcatc gctgtacggc cgcatcgccg ccggcgccga gaaccccgaa cccggccgct acggcggcga agttgccgtg gtgaccggcg cttcgaaggg ttcgatcgcc gcgtcggtgg tggctcggct gctcgacggc ggagccaccg tcatcgcgac cacctccaag ctcgacgagg agcggctggc gttctaccgc acgctgtatc gcgaccacgc ccgttacggc gcggcgctgt ggctggtcgc ggcgaacatg gcgtcctact ccgacgtcga cgccctggtc gaatggatcg gcaccgaaca gaccgaaagc cttgggccgc agtcgattca catcaaagac gcgcagaccc cgacgctgct gttcccgttc gcggcgccac gcgtggtcgg ggacctgtcg gaggccggtt cgcgcgccga gatggagatg aaagtgctgc tgtgggccgt gcaacggctg atcggcggcc tgtcgacgat cggcgccgaa cgcgacatcg cgtcgcggct gcacgtggtg ctgcccggct cgcccaaccg tggcatgttc ggcggcgacg gcgcctacgg cgaagccaag tccgcgctgg atgccgtggt gagccgctgg cacgccgagt cgtcctgggc ggcacgggtc agcctggcgc acgcgctcat cggctggacc cgcggcaccg ggctgatggg ccacaacgat gccatcgtgg ccgccgtcga agaggccggg gtcaccacct actcgaccga cgagatggcg gcgctgctgc tcgacctgtg tgatgcggaa tccaaggtgg ctgcggcgcg ttcgccgatc aaggccgacc tgaccggggg cctggccgag gccaacctcg acatggccga gctggcggcc aaggcgcgcg agcagatgtc ggcagcggcg gccgtcgacg aggacgccga ggcccctggc gccatcgccg cgctgccgtc gccgccccgg ggtttcaccc ccgcaccgcc gccgcaatgg gacgacctcg atgtcgaccc ggccgacctg gtggtgatcg tcggcggcgc cgaaatcggc ccgtacggct cgtcacgcac ccggttcgag atggaggtcg aaaacgagct gtcggcggcc ggcgtgctgg agctggcctg gaccactggg ttgatccgct gggaggacga cccgcaaccc ggttggtacg acaccgaatc cggcgaaatg gtcgacgaat ccgagttggt gcagcgctac cacgacgccg tggtgcagcg cgtcggcatt cgcgaattcg ttgatgacgg cgcgatcgac cccgaccacg cctcgccgct gctggtgtcg gtgttcctgg agaaggactt cgcgttcgtg gtgtcctcgg aggccgatgc gcgcgccttc gtcgagttcg atcccgagca cacggtcatc cggccggtgc ccgactccac cgactggcag gtcatccgca aggccggcac cgagatccgg gtgccgcgaa agaccaagct gtcccgcgtc gtcggcggcc agatcccgac cgggttcgac ccgacggtgt ggggcatcag cgcagacatg gccggttcca tcgaccggtt ggcggtatgg aacatggtgg cgaccgtcga cgcgttcctg tcgtccggtt tcagcccggc cgaggtgatg cgttacgtgc acccgagttt ggtggccaac acccagggca ccggcatggg cggcggcacg tcgatgcaga cgatgtacca cggcaatctg ttgggccgca acaagccgaa cgacatcttc caggaagtct tgccgaatat cattgccgcg cacgtggttc agtcctacgt cggtagctac ggtgcgatga tccacccggt agccgcgtgc gccaccgccg cggtgtcggt cgaggaaggt gtcgacaaga tccggttggg caaggctcaa ctggtggtgg ccggcggcct ggatgacctg acgctggagg gcatcatcgg attcggtgac atggccgcca ccgccgacac gtccatgatg tgcggccgcg gcatccacga ctcgaagttt tcccggccca acgaccgccg ccgtctgggc ttcgtcgaag cccaaggcgg cgggacgatc ctgttggccc gcggggacct ggcgctgcgg atggggctgc cggtgctggc ggtggtggcg ttcgcgcagt cgttcggcga cggcgtgcac acctcgatcc cggccccggg cctgggcgcg ctgggggcgg gccgcggcgg caaggattca ccgctggcgc gggcgctggc caagctgggc gtggccgccg acgacgtggc ggtcatctcc aagcacgaca cctcgacgct ggccaacgat cccaacgaga ccgagttgca tgaacggctc gccgacgccc tgggccgttc cgagggcgcc ccgctgttcg tggtgtcgca gaagagcctg accggccacg ccaagggcgg cgcggcggtc ttccagatga tggggctctg ccagatattg cgggatgggg tgatcccacc caaccgcagc ctcgactgcg tcgacgacga gctggccggc tccgcgcatt tcgtgtgggt gcgtgacacg ttgcggctcg gcggcaagtt cccactcaag gccggcatgc tgaccagcct cgggttcggc catgtgtcgg gcctggtcgc gttggtgcat ccgcaggcgt tcatcgcctc gctggatccc gcacagcgcg cggactacca gcggcgtgcc gacgcccgcc tgctggccgg tcagcgccgg ctggcctcgg cgattgccgg tggtgcgccg atgtaccagc ggcccggtga ccgtcgcttc gaccaccacg cgcccgagcg gccgcaggag gcgtcgatgc tgctgaatcc ggcggcccgg ctgggtgacg gcgaggcgta tatcggctga
Details for MytuD.20412.a.B2.GE43838
HARVESTED ON: data unavailable
SEQUENCED ON: 8/28/2020
EXPECTED MW: 14kDa
OBSERVED MW: 73kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD VTITAPVDMR PFAVVSGDHN PIHTDRAAAL LAGLESPIVH GMWLSAAAQH AVTATDGQAR PPARLVGWTA RFLGMVRPGD EVDFRVERVG IDQGAEIVDV AARVGSDLVM SXSARLAAP
Validated NT Sequence
atggctcacc accaccacca ccatatggac gtcacgatca ccgcgccggt cgacatgcgc ccgttcgcgg tggtgtccgg cgaccacaac cccattcaca ccgaccgggc cgccgcgctg cttgccggcc tggagtcgcc gatcgtgcac ggcatgtggc tgtcggccgc ggcgcaacac gcggtgaccg ccaccgacgg gcaggcccgg ccaccggccc ggctggtcgg ctggaccgcg cggtttttgg gcatggtgcg ccccggcgac gaggtggact tccgcgtcga gcgcgtcgga atcgaccagg gcgcagagat tgtggacgtg gccgcgcgcg tcgggtcgga tctagtgatg tcggnctccg cgcgactggc cgcaccctaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHDV TITAPVDMRP FAVVSGDHNP IHTDRAAALL AGLESPIVHG MWLSAAAQHA VTATDGQARP PARLVGWTAR FLGMVRPGDE VDFRVERVGI DQGAEIVDVA ARVGSDLVMS ASARLAAP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgtca cgatcaccgc gccggtcgac atgcgcccgt tcgcggtggt gtccggcgac cacaacccca ttcacaccga ccgggccgcc gcgctgcttg ccggcctgga gtcgccgatc gtgcacggca tgtggctgtc ggccgcggcg caacacgcgg tgaccgccac cgacgggcag gcccggccac cggcccggct ggtcggctgg accgcgcggt ttttgggcat ggtgcgcccc ggcgacgagg tggacttccg cgtcgagcgc gtcggaatcg accagggcgc agagattgtg gacgtggccg cgcgcgtcgg gtcggatcta gtgatgtcgg cctccgcgcg actggccgca ccctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.20412.a.B3.GE43841
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 57kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMD VTITAPVDMR PFAVVSGDHN PIHTDRAAAL LAGLESPIVH GMWLSAAAQH AVTATDGQAR PPARLVGWTA RFLGMVRPGD EVDFRVERVG IDQGAEIVDV AARVGSDLVM SASARLAAPK TVYAFPGQGI QHKGMGMEVR ARSKAARKVW DTADKFTRDT LGFSVLHVVR DNPTSIIASG VHYHHPDGVL YLTQFTQVAM ATVAAAXVAE MREQGXFVEX AIACXXSVGE YTALACVTGI YQLEALLEMV FHRGSKMHDI VPRDELGRSN YRLAAIRPSQ IDLDDADVPA FVAGIAESTG EFLEIVNFNL RGSQYAIAGT VRGLEALEAE VERRRELTGG RRSFILVPGI DVPFHSRVLR VGVAEFRRSL DRVMPRDADP DLIIGRYIPN LVPRLFTLDR DFIQEIRDLV PAEPLDEILA DYDTWLRERP REMARTVFIE LLAWQFASPV RWIETQDLLF IEEAAGGLGV ERFVEIGVKS SPTVAGLATN TLKLPEYAHS TV
Validated NT Sequence
atggctcacc accaccacca ccatatggac gtcacgatca ccgcgccggt cgacatgcgc ccgttcgcgg tggtgtccgg cgaccacaac cccattcaca ccgaccgggc cgccgcgctg cttgccggcc tggagtcgcc gatcgtgcac ggcatgtggc tgtcggccgc ggcgcaacac gcggtgaccg ccaccgacgg gcaggcccgg ccaccggccc ggctggtcgg ctggaccgcg cggtttttgg gcatggtgcg ccccggcgac gaggtggact tccgcgtcga gcgcgtcgga atcgaccagg gcgcagagat tgtggacgtg gccgcgcgcg tcgggtcgga tctagtgatg tcggcctccg cgcgactggc cgcacccaag acggtctacg cattccccgg ccagggcatc caacacaagg gcatgggcat ggaggtgcgc gcccgctcca aggcggcccg caaggtgtgg gacaccgcgg acaagttcac ccgcgacacc ctgggcttct cggtactgca cgtggtccgc gacaacccga ccagcatcat cgccagcggt gtgcactacc accaccccga cggggtgctc tacctgacgc agttcaccca ggtcgcgatg gcgacngtgg cggccgcgca ngtcgccgag atgcgtgaac agggancctt cgtcgaangn gccatcgcgt gcgnnnactc ggtcggcgag tacaccgcgc tggcctgcgt gaccggcatc taccaactgg aagccttgct ggagatggtg tttcaccgcg ggtcgaagat gcacgacatc gttccgcgcg acgagctcgg ccgctccaac tatcggctgg cggccatccg gccgtcccag atcgacctcg acgacgccga cgtgcccgcg ttcgtcgccg ggatcgcgga gagcaccggt gaattcctgg agatcgtgaa tttcaacctg cgtggctcgc aatacgcgat cgcgggcacg gtacgcggcc tcgaggcgct cgaggccgag gtggagcggc gccgcgagct caccggcggc cgacggtcgt tcattttggt gcccggcatc gatgttccgt tccactcgcg agtgctgcgg gtcggggtgg ccgaattccg gcgctcgctg gaccgggtca tgccgcgcga cgcggacccc gacctgatca tcgggcgcta cattcccaac ctggtgccgc ggttgttcac cctggaccgc gacttcatcc aggaaatccg ggatttggtg cccgccgagc cgctcgacga gatcctcgcc gactacgaca cctggcttcg cgagcgtccg cgcgagatgg cgcgcacggt gttcatcgag ctgctggcat ggcaattcgc cagcccggtg cgctggatcg agacgcagga tctgctgttc atcgaggagg ccgccggcgg gctgggtgtg gagcgattcg tcgagatcgg tgtgaagagc tcaccgacgg tggcgggtct tgccaccaac accctcaaac tgcccgaata cgcccacagc acagtggaa
Expected Protein Sequence
MAHHHHHHDV TITAPVDMRP FAVVSGDHNP IHTDRAAALL AGLESPIVHG MWLSAAAQHA VTATDGQARP PARLVGWTAR FLGMVRPGDE VDFRVERVGI DQGAEIVDVA ARVGSDLVMS ASARLAAPKT VYAFPGQGIQ HKGMGMEVRA RSKAARKVWD TADKFTRDTL GFSVLHVVRD NPTSIIASGV HYHHPDGVLY LTQFTQVAMA TVAAAQVAEM REQGAFVEGA IACGHSVGEY TALACVTGIY QLEALLEMVF HRGSKMHDIV PRDELGRSNY RLAAIRPSQI DLDDADVPAF VAGIAESTGE FLEIVNFNLR GSQYAIAGTV RGLEALEAEV ERRRELTGGR RSFILVPGID VPFHSRVLRV GVAEFRRSLD RVMPRDADPD LIIGRYIPNL VPRLFTLDRD FIQEIRDLVP AEPLDEILAD YDTWLRERPR EMARTVFIEL LAWQFASPVR WIETQDLLFI EEAAGGLGVE RFVEIGVKSS PTVAGLATNT LKLPEYAHST VEVLNAERDA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgtca cgatcaccgc gccggtcgac atgcgcccgt tcgcggtggt gtccggcgac cacaacccca ttcacaccga ccgggccgcc gcgctgcttg ccggcctgga gtcgccgatc gtgcacggca tgtggctgtc ggccgcggcg caacacgcgg tgaccgccac cgacgggcag gcccggccac cggcccggct ggtcggctgg accgcgcggt ttttgggcat ggtgcgcccc ggcgacgagg tggacttccg cgtcgagcgc gtcggaatcg accagggcgc agagattgtg gacgtggccg cgcgcgtcgg gtcggatcta gtgatgtcgg cctccgcgcg actggccgca cccaagacgg tctacgcatt ccccggccag ggcatccaac acaagggcat gggcatggag gtgcgcgccc gctccaaggc ggcccgcaag gtgtgggaca ccgcggacaa gttcacccgc gacaccctgg gcttctcggt actgcacgtg gtccgcgaca acccgaccag catcatcgcc agcggtgtgc actaccacca ccccgacggg gtgctctacc tgacgcagtt cacccaggtc gcgatggcga cggtggcggc cgcgcaggtc gccgagatgc gtgaacaggg agccttcgtc gaaggcgcca tcgcgtgcgg ccactcggtc ggcgagtaca ccgcgctggc ctgcgtgacc ggcatctacc aactggaagc cttgctggag atggtgtttc accgcgggtc gaagatgcac gacatcgttc cgcgcgacga gctcggccgc tccaactatc ggctggcggc catccggccg tcccagatcg acctcgacga cgccgacgtg cccgcgttcg tcgccgggat cgcggagagc accggtgaat tcctggagat cgtgaatttc aacctgcgtg gctcgcaata cgcgatcgcg ggcacggtac gcggcctcga ggcgctcgag gccgaggtgg agcggcgccg cgagctcacc ggcggccgac ggtcgttcat tttggtgccc ggcatcgatg ttccgttcca ctcgcgagtg ctgcgggtcg gggtggccga attccggcgc tcgctggacc gggtcatgcc gcgcgacgcg gaccccgacc tgatcatcgg gcgctacatt cccaacctgg tgccgcggtt gttcaccctg gaccgcgact tcatccagga aatccgggat ttggtgcccg ccgagccgct cgacgagatc ctcgccgact acgacacctg gcttcgcgag cgtccgcgcg agatggcgcg cacggtgttc atcgagctgc tggcatggca attcgccagc ccggtgcgct ggatcgagac gcaggatctg ctgttcatcg aggaggccgc cggcgggctg ggtgtggagc gattcgtcga gatcggtgtg aagagctcac cgacggtggc gggtcttgcc accaacaccc tcaaactgcc cgaatacgcc cacagcacag tggaagtgct caacgccgag cgtgatgcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for MytuD.20412.a.MB2.GE43840
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 38
Validated AA Sequence
MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTNSSS NNNNNNNNNN LGIEGRISEG TLEAQTQGPG SMDVTITAPV DMRPFAVVSG DHNPIHTDRA AALLAGLESP IVHGMWLSAA AQHAVTATDG QARPPARLVG WTARFLGMVR PGDEVDFRVE RVGIDQGAEI VDVAARVGSD LVMSASARLA AP
Validated NT Sequence
ttnnttnnnn ntttgttagc agccggatcc tcgagaagct tggctgcaga acttgttcgt gctgtttatt agggtgcggc cagtcgcgcg gaggccgaca tcactagatc cgacccgacg cgcgcggcca cgtccacaat ctctgcgccc tggtcgattc cgacgcgctc gacgcggaag tccacctcgt cgccggggcg caccatgccc aaaaaccgcg cggtccagcc gaccagccgg gccggtggcc gggcctgccc gtcggtggcg gtcaccgcgt gttgcgccgc ggccgacagc cacatgccgt gcacgatcgg cgactccagg ccggcaagca gcgcggcggc ccggtcggtg tgaatggggt tgtggtcgcc ggacaccacc gcgaacgggc gcatgtcgac cggcgcggtg atcgtgacgt ccatcgaacc aggaccctgg gtctgagctt ccagggtacc ttctgaaatc cttccctcga tcccgaggtt gttgttattg ttgttgttgt tgttgttcga gctcgaatta gtctgcgcgt ctttcagggc ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatagt ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ctacggcacc cagcggtttg tctttattaa ccgcttccag accttcatca gtcagcagat agttttcgag gaactctttt gccagctctt tgttcggact ggcggcgttt atacctgcgc tcagcacgcc aacgaacggn ttggatggnt ngacccttga aggtcggcag taccgttaca ccataattca ctttgctggn gncgatgttg gaccntgccc acnggnngnt gatggtcatc gnntnnnttc nnccnttnan tannnagctt nnngcgatgg aagtaatnnn nnnnnntgca ttcatgnnnn tttgntttna atnnnngnca ncnngaangn nnnnaccnnc nttnncnncn gcntnntccn nnccc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS DVTITAPVDM RPFAVVSGDH NPIHTDRAAA LLAGLESPIV HGMWLSAAAQ HAVTATDGQA RPPARLVGWT ARFLGMVRPG DEVDFRVERV GIDQGAEIVD VAARVGSDLV MSASARLAAP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggacgt cacgatcacc gcgccggtcg acatgcgccc gttcgcggtg gtgtccggcg accacaaccc cattcacacc gaccgggccg ccgcgctgct tgccggcctg gagtcgccga tcgtgcacgg catgtggctg tcggccgcgg cgcaacacgc ggtgaccgcc accgacgggc aggcccggcc accggcccgg ctggtcggct ggaccgcgcg gtttttgggc atggtgcgcc ccggcgacga ggtggacttc cgcgtcgagc gcgtcggaat cgaccagggc gcagagattg tggacgtggc cgcgcgcgtc gggtcggatc tagtgatgtc ggcctccgcg cgactggccg caccctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MytuD.20412.a.MB3.GE43842
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 101kDa
OBSERVED MW: 101kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 38
PERCENT COVERAGE: 15
Validated AA Sequence
MIRSGSASRG MTRSSERRNS ATPTRSTREW NGTSMPGTKM NDRRPPVSSR RRSTSASSAS RXRTVPAIAY CEPRRLKFTI SRNSPVLSAI PATNAGTSAS SRSIWDGRMA PADSWSGRAR RAERCRASST RGETPSPARL PVW
Validated NT Sequence
tnccttnntn nnnctttgtt agcagccgga tcctcgagaa gcttggctgc agaacttgtt cgtgctgttt attaggcatc acgctcggcg ttgagcactt ccactgtgct gtgggcgtat tcgggcagtt tgagggtgtt ggtggcaaga cccgccaccg tcggtgagct cttcacaccg atctcgacga atcgctccac acccagcccg ccggcggcct cctcgatgaa cagcagatcc tgcgtctcga tccagcgcac cgggctggcg aattgccatg ccagcagctc gatgaacacc gtgcgcgcca tctcgcgcgg acgctcgcga agccaggtgt cgtagtcggc gaggatctcg tcgagcggct cggcgggcac caaatcccgg atttcctgga tgaagtcgcg gtccagggtg aacaaccgcg gcaccaggtt gggaatgtag cgcccgatga tcaggtcggg gtccgcgtcg cgcggcatga cccggtccag cgagcgccgg aattcggcca ccccgacccg cagcactcgc gagtggaacg gaacatcgat gccgggcacc aaaatgaacg accgtcggcc gccggtgagc tcgcggcgcc gctccacctc ggcctcgagc gcctcgaggn cgcgtaccgt gcccgcgatc gcgtattgcg agccacgcag gttgaaattc acgatctcca ggaattcacc ggtgctctcc gcgatcccgg cgacgaacgc gggcacgtcg gcgtcgtcna ggtcgatctg ggacggncgg atggcnccag ccgatagttg gagcggccga gctcgtcgcg cggaacgatg tcgtgcatct tcnacccgcg gtgaaacacc atctccagca aggcttccag tttggtagat gcccggtcac gcanncancg cgggtgtact cnnnancnan tggccgcacg cgatggnnnn tnngacgann ntcctngntt cangcatctc ggactgnnnc ngncgcncnt nnnnntcgnn gacnnnggtg nactgcgtcn gannnncccc ntcngnnggn ngnangnnnn ncngntgnna nnannctnnn nnngnnnnnn ncagnnncnn nancnnggnn nnngggnnaa nttnnncnnn nnnnccnnnn nnnnnng
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS DVTITAPVDM RPFAVVSGDH NPIHTDRAAA LLAGLESPIV HGMWLSAAAQ HAVTATDGQA RPPARLVGWT ARFLGMVRPG DEVDFRVERV GIDQGAEIVD VAARVGSDLV MSASARLAAP KTVYAFPGQG IQHKGMGMEV RARSKAARKV WDTADKFTRD TLGFSVLHVV RDNPTSIIAS GVHYHHPDGV LYLTQFTQVA MATVAAAQVA EMREQGAFVE GAIACGHSVG EYTALACVTG IYQLEALLEM VFHRGSKMHD IVPRDELGRS NYRLAAIRPS QIDLDDADVP AFVAGIAEST GEFLEIVNFN LRGSQYAIAG TVRGLEALEA EVERRRELTG GRRSFILVPG IDVPFHSRVL RVGVAEFRRS LDRVMPRDAD PDLIIGRYIP NLVPRLFTLD RDFIQEIRDL VPAEPLDEIL ADYDTWLRER PREMARTVFI ELLAWQFASP VRWIETQDLL FIEEAAGGLG VERFVEIGVK SSPTVAGLAT NTLKLPEYAH STVEVLNAER DA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggacgt cacgatcacc gcgccggtcg acatgcgccc gttcgcggtg gtgtccggcg accacaaccc cattcacacc gaccgggccg ccgcgctgct tgccggcctg gagtcgccga tcgtgcacgg catgtggctg tcggccgcgg cgcaacacgc ggtgaccgcc accgacgggc aggcccggcc accggcccgg ctggtcggct ggaccgcgcg gtttttgggc atggtgcgcc ccggcgacga ggtggacttc cgcgtcgagc gcgtcggaat cgaccagggc gcagagattg tggacgtggc cgcgcgcgtc gggtcggatc tagtgatgtc ggcctccgcg cgactggccg cacccaagac ggtctacgca ttccccggcc agggcatcca acacaagggc atgggcatgg aggtgcgcgc ccgctccaag gcggcccgca aggtgtggga caccgcggac aagttcaccc gcgacaccct gggcttctcg gtactgcacg tggtccgcga caacccgacc agcatcatcg ccagcggtgt gcactaccac caccccgacg gggtgctcta cctgacgcag ttcacccagg tcgcgatggc gacggtggcg gccgcgcagg tcgccgagat gcgtgaacag ggagccttcg tcgaaggcgc catcgcgtgc ggccactcgg tcggcgagta caccgcgctg gcctgcgtga ccggcatcta ccaactggaa gccttgctgg agatggtgtt tcaccgcggg tcgaagatgc acgacatcgt tccgcgcgac gagctcggcc gctccaacta tcggctggcg gccatccggc cgtcccagat cgacctcgac gacgccgacg tgcccgcgtt cgtcgccggg atcgcggaga gcaccggtga attcctggag atcgtgaatt tcaacctgcg tggctcgcaa tacgcgatcg cgggcacggt acgcggcctc gaggcgctcg aggccgaggt ggagcggcgc cgcgagctca ccggcggccg acggtcgttc attttggtgc ccggcatcga tgttccgttc cactcgcgag tgctgcgggt cggggtggcc gaattccggc gctcgctgga ccgggtcatg ccgcgcgacg cggaccccga cctgatcatc gggcgctaca ttcccaacct ggtgccgcgg ttgttcaccc tggaccgcga cttcatccag gaaatccggg atttggtgcc cgccgagccg ctcgacgaga tcctcgccga ctacgacacc tggcttcgcg agcgtccgcg cgagatggcg cgcacggtgt tcatcgagct gctggcatgg caattcgcca gcccggtgcg ctggatcgag acgcaggatc tgctgttcat cgaggaggcc gccggcgggc tgggtgtgga gcgattcgtc gagatcggtg tgaagagctc accgacggtg gcgggtcttg ccaccaacac cctcaaactg cccgaatacg cccacagcac agtggaagtg ctcaacgccg agcgtgatgc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa