MytuD.20406.a

Polyketide synthase Pks13 (Rv3800c)

CENTER ID: MytuD.20406.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.20406.a.B6.GE43836 construct 5 25 539
MytuD.20406.a.B7.GE43837 construct 6 7 582
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|83332.111.peg.4225
TubercuList: Rv3800c
MTB Network Portal: Rv3800c
UniProt: I6X8D2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADVAESQEN APAERAELTV PEMRQWLRNW VGKAVGKAPD SIDESVPMVE LGLSSRDAVA MAADIEDLTG VTLSVAVAFA HPTIESLATR IIEGEPETDL AGDDAEDWSR TGPAERVDIA IVGLSTRFPG EMNTPEQTWQ ALLEGRDGIT DLPDGRWSEF LEEPRLAARV AGARTRGGYL KDIKGFDSEF FAVAKTEADN IDPQQRMALE LTWEALEHAR IPASSLRGQA VGVYIGSSTN DYSFLAVSDP TVAHPYAITG TSSSIIANRV SYFYDFHGPS VTIDTACSSS LVAIHQGVQA LRNGEADVVV AGGVNALITP MVTLGFDEIG AVLAPDGRIK SFSADADGYT RSEGGGMLVL KRVDDARRDG DAILAVIAGS AVNHDGRSNG LIAPNQDAQA DVLRRAYKDA GIDPRTVDYI EAHGTGTILG DPIEAEALGR VVGRGRPADR PALLGAVKTN VGHLESAAGA ASMAKVVLAL QHDKLPPSIN FAGPSPYIDF DAMRLKMITT PTDWPRYGGY ALAGVSSFGF GGANAHVVVR EVLPRDVVEK EPEPEPEPKA AAEPAEAPTL AGHALRFDEF GNIITDSAVA EEPEPELPGV TEEALRLKEA ALEELAAQEV TAPLVPLAVS AFLTSRKKAA AAELADWMQS PEGQASSLES IGRSLSRRNH GRSRAVVLAH DHDEAIKGLR AVAAGKQAPN VFSVDGPVTT GPVWVLAGFG AQHRKMGKSL YLRNEVFAAW IEKVDALVQD ELGYSVLELI LDDAQDYGIE TTQVTIFAIQ IALGELLRHH GAKPAAVIGQ SLGEAASAYF AGGLSLRDAT RAICSRSHLM GEGEAMLFGE YIRLMALVEY SADEIREVFS DFPDLEVCVY AAPTQTVIGG PPEQVDAILA RAEAEGKFAR KFATKGASHT SQMDPLLGEL TAELQGIKPT SPTCGIFSTV HEGRYIKPGG EPIHDVEYWK KGLRHSVYFT HGIRNAVDSG HTTFLELAPN PVALMQVALT TADAGLHDAQ LIPTLARKQD EVSSMVSTMA QLYVYGHDLD IRTLFSRASG PQDYANIPPT RFKRKEHWLP AHFSGDGSTY MPGTHVALPD GRHVWEYAPR DGNVDLAALV RAAAAHVLPD AQLTAAEQRA VPGDGARLVT TMTRHPGGAS VQVHARIDES FTLVYDALVS RAGSESVLPT AVGAATAIAV ADGAPVAPET PAEDADAETL SDSLTTRYMP SGMTRWSPDS GETIAERLGL IVGSAMGYEP EDLPWEVPLI ELGLDSLMAV RIKNRVEYDF DLPPIQLTAV RDANLYNVEK LIEYAVEHRD EVQQLHEHQK TQTAEEIARA QAELLHGKVG KTEPVDSEAG VALPSPQNGE QPNPTGPALN VDVPPRDAAE RVTFATWAIV TGKSPGGIFN ELPRLDDEAA AKIAQRLSER AEGPITAEDV LTSSNIEALA DKVRTYLEAG QIDGFVRTLR ARPEAGGKVP VFVFHPAGGS TVVYEPLLGR LPADTPMYGF ERVEGSIEER AQQYVPKLIE MQGDGPYVLV GWSLGGVLAY ACAIGLRRLG KDVRFVGLID AVRAGEEIPQ TKEEIRKRWD RYAAFAEKTF NVTIPAIPYE QLEELDDEGQ VRFVLDAVSQ SGVQIPAGII EHQRTSYLDN RAIDTAQIQP YDGHVTLYMA DRYHDDAIMF EPRYAVRQPD GGWGEYVSDL EVVPIGGEHI QAIDEPIIAK VGEHMSRALG QIEADRTSEV GKQ
NT Sequence
atggctgacg tagcggaatc ccaggagaac gcccccgccg aaagggccga gctaacggtc cccgagatgc gccagtggct gcgcaactgg gtgggtaagg ccgtcggaaa ggcaccggac tcgatcgacg aatcggtgcc catggtggag ctgggtctgt cgtcgcgcga tgccgtcgcg atggccgccg acatagaaga cctgaccggg gtcacgctgt cggtcgcggt ggcgttcgcg catccgacca tcgaatcgct ggccacccgg atcatcgagg gcgagccgga gaccgaccta gcgggcgatg acgccgaaga ctggtcgcgc accggcccgg ccgagcgcgt cgacatcgcg atcgtgggct tgtccacccg cttcccgggc gagatgaaca cccccgagca gacctggcag gcgctgctgg aaggccgcga cgggatcacc gacctgcccg acgggcgctg gtcggaattc ctcgaagagc cgcggctggc cgcgcgggtc gccggggccc gcacccgggg cggctacctg aaggacatca agggcttcga ttcggagttc ttcgcggtgg ccaagaccga agccgacaac atcgacccgc agcagcggat ggcgctggag ctgacctggg aggcgctcga gcacgcccgc atcccggcgt cgagcctgcg cggccaggcc gtcggtgtgt acatcggcag ctccaccaac gactacagct tcctggcggt gtcggacccg acggtcgcgc acccgtatgc gatcaccggc accagcagct cgatcatcgc caaccgggtg tcctacttct acgacttcca cggaccgtcg gtcaccattg acaccgcgtg ctcgagttcg ctggtggcca tccaccaggg ggtgcaggcg ctgcgcaacg gcgaggccga cgtagtggtc gccggcgggg tgaacgcgtt gatcacaccg atggtcaccc tgggtttcga cgagatcggt gcggtgctgg cgcccgacgg ccggatcaag tcgttctcag ccgacgccga cggctacacc cgctccgaag gcggcggcat gctggtgctc aagcgggtcg acgacgcccg ccgcgacggc gacgcgatcc tggccgtgat cgccggcagc gcggtcaacc acgacggccg gtccaacggc ctgatcgcac ccaaccagga cgcgcaggcc gacgtgctgc gccgggccta caaggacgcc ggcatcgatc cgcgcaccgt cgactacatc gaggcgcacg gcaccggcac catcctcggc gacccaatcg aggccgaggc gctgggccgg gtggtcggta ggggccgtcc ggccgatcgg ccggcgctgc tgggtgcggt gaaaaccaac gtcgggcacc tggaatcggc ggccggcgcg gccagcatgg ccaaggtggt gctggcgctg cagcacgaca aactgccgcc gtcgatcaac ttcgccggcc ccagccccta catcgacttc gacgcgatgc ggttgaagat gatcaccacg cccaccgact ggccgcgata cggcggctac gcgctggccg gggtgtccag cttcggcttc ggcggcgcca acgcgcacgt ggtggtgcgc gaggtcctgc cgcgtgacgt ggtggaaaag gaaccggaac ccgagccgga acccaaggcg gccgccgaac ccgccgaggc gcccacgttg gcaggccacg cgctgcggtt cgacgagttc ggcaacatca tcaccgactc ggcggtcgcc gaagagccgg agcccgaact gcccggagtc accgaggagg cgctgcggct caaggaagcc gcgttggaag agcttgcggc ccaagaggtt acggcaccat tggtcccgtt ggcggtgtcg gcgtttctga cgtcccgcaa gaaggcggcg gccgccgagt tggcggactg gatgcaaagc ccggaaggcc aggcctcctc gctggaatcg atcggcaggt cgttgtcgcg gcgcaaccac ggccgttccc gcgcggtggt gttggcccac gaccacgacg aggccatcaa gggcctgcgc gcggtcgccg cgggcaagca ggcgccgaac gtgttcagcg tcgacgggcc ggtgaccacc ggcccggtct gggtgctcgc cggattcggc gcccagcatc gcaagatggg caagagcctg tacctgcgca acgaggtgtt cgcggcgtgg atcgagaagg tcgacgccct ggtccaagac gagctgggct actcggtgct ggagctgatc ctggacgacg cgcaggacta cggcatcgag accacccagg tcaccatctt cgcgatccag atcgcgctgg gtgagctgct gcgccatcac ggcgccaaac cggccgcggt catcggccag tcgctgggtg aggccgcgtc ggcctacttc gccggcgggc tgtcgctgcg ggatgccacc cgggcgatct gctcgcgctc gcacctgatg ggcgagggtg aggcgatgct gttcggcgag tacatccggt tgatggcgct ggtggaatac tccgccgacg aaatcagaga agtgttctcc gacttccccg atctggaggt gtgtgtctac gccgcgccca cccagacggt catcggcggc ccccccgagc aggtggacgc gatccttgcc cgcgccgagg ccgagggcaa gttcgcccgc aaattcgcga ccaagggcgc cagccacacc tcgcagatgg acccgctgct gggcgagctc accgcggagc tgcaaggcat caagccgacg agcccgacgt gtgggatctt ctcgacggtg cacgagggcc gctacatcaa acccggcggc gaacccatcc acgacgtcga atactggaag aaggggctgc ggcattccgt ctacttcacc cacggcatcc gcaacgccgt cgacagcggg cacaccacct tcctggagct ggcacccaat ccggtggcgc tgatgcaggt cgccctgacc accgccgatg ccgggctgca tgacgcccag ttgatcccga cgctggcccg caagcaagac gaggtctcct cgatggtctc gaccatggcg cagctgtatg tgtacggcca cgacctggac atacgcacgc tgtttagccg cgccagtggg ccgcaggatt acgcgaacat tccgccgacc cggttcaagc gcaaggagca ctggctgccc gcgcacttct ccggcgacgg ctcgacgtac atgccgggca cccatgtcgc cctgccggat gggcggcacg tctgggagta cgcgccgcgg gacggcaatg tggacttggc cgcgttggtc agggccgccg ccgcccacgt gcttccggac gcgcaactga ccgccgccga gcagcgcgcg gtgcccggcg acggcgcccg gctggtgacg acgatgaccc gtcaccccgg cggcgcctcg gtgcaggtgc acgcccgcat cgacgagtcc ttcacgctgg tctacgacgc cctggtgtcc cgagcggggt ccgaatcggt gttgcccacc gcggtgggtg cggcgacggc gatcgcggtt gcggacgggg cgcctgtcgc gccggaaacg cccgccgaag acgcggacgc cgagacgctt tcggacagcc tgaccacccg ttacatgccg tccggcatga cccgatggtc gcctgattcc ggtgagacca tcgccgagcg gctgggcctg attgtcgggt ctgcgatggg ctatgagccc gaggacctgc cgtgggaggt gccgctgatc gagcttggcc tggactcgct gatggcggtg cgcatcaaaa accgcgtcga gtacgacttc gacctgccac cgatccagct gaccgcggtg cgcgacgcca acctctacaa cgtggagaag ctgatcgaat acgcggtcga gcaccgtgac gaggtgcagc agctgcacga gcaccagaaa acccagaccg ctgaggagat cgcgcgggcc caggccgaat tgctgcatgg caaggtgggc aagaccgagc cggtcgactc ggaagccggg gttgcgctcc cgtcgccgca aaacggcgag cagccaaacc cgacagggcc cgcgctcaac gtcgacgtgc cgccgcggga cgctgccgag cgggtcacct tcgccacctg ggcgatcgtc accggcaagt ccccgggcgg catcttcaac gagctgccca ggctggacga cgaggccgcg gccaagattg cgcagcggct ttccgagcgc gccgaaggcc cgatcaccgc cgaggacgtg ctgacgtcgt cgaacatcga ggcgctggcc gacaaggtgc gcacgtattt ggaggccggg cagatcgatg ggttcgtccg caccctgcgg gcgcggcccg aagcaggcgg gaaggtgccg gtgttcgtgt ttcatccggc cggcggctcg acggtggtgt acgagccgct gctgggccgg ctgccggcgg acaccccaat gtatggcttc gaacgggtcg aggggtcgat cgaagagcgt gcacagcagt acgtgccgaa gctgatcgag atgcagggcg acgggcccta tgtcctggtg ggttggtcgc tgggcggtgt gctggcctac gcgtgcgcga tcggtttgcg gcggctgggc aaggacgtgc ggttcgtcgg gctgatcgac gcggtgcgcg ccggtgagga gatcccgcag accaaggagg agatccgcaa gcgctgggac cgctacgccg ccttcgccga gaagacgttc aacgtgacca tcccggcgat cccgtacgag cagctcgagg agctcgacga cgagggccag gtccggttcg tgctggacgc cgtcagccag tccggtgtgc agatcccggc cgggatcatc gaacaccaac gcacgtcgta tctggacaac cgggcgatcg acaccgccca gatccagccg tacgacgggc atgtcaccct ctacatggcc gatcgctacc atgacgacgc gatcatgttc gagccccgct acgccgtgcg ccagccggac ggcgggtggg gcgagtacgt ttccgacctc gaggtggtgc cgatcggtgg cgagcacatt caggccatcg acgagccgat catcgccaag gtgggcgaac acatgagccg cgcgttgggg cagatcgagg ccgatcgaac aagtgaggta ggcaagcagt ga
Details for MytuD.20406.a.B6.GE43836
HARVESTED ON: 8/25/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHQW LRNWVGKAVG KAPDSIDESV PMVELGLSSR DAVAMAADIE DLTGVTLSVA VAFAHPTIES LATRIIEGEP ETDLAGDDAE DWSRTGPAER VDIAIVGLST RFPGEMNTPE QTWQALLEGR DGITDLPDGR WSEFLEEPRL AARVAGARTR GGYLKDIKGF DSEFFAVAKT EADNIDPQQR MALELTWEAL EHARIPASSL RGQAVGVYIG SSTNDYSFLA VSDPTVAHPY AITGTSSSII ANRVSYFYDF HGPSVTIDTA CSSSLVAIHQ GVQALRNGEA DVVVAGGVNA LITPMVTLGF DEIGAVLAPD GRIKSFSADA DGYTRSEGGG MLVLKRVDDA RRDGDAILAV IAGSAVNHDG RSNGLIAPNQ DAQADVLRRA YKDAGIDPRT VDYIEAHGTG TILGDPIEAE ALGRVVGRGR PADRPALLGA VKTNVGHLES AAGAASMAKV VLALQHDKLP PSINFAGPSP YIDFDAMRLK MITTPTDWPR YGGYALAGVS SFGFGGANAH VVV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagtggc tgcgcaactg ggtgggtaag gccgtcggaa aggcaccgga ctcgatcgac gaatcggtgc ccatggtgga gctgggtctg tcgtcgcgcg atgccgtcgc gatggccgcc gacatagaag acctgaccgg ggtcacgctg tcggtcgcgg tggcgttcgc gcatccgacc atcgaatcgc tggccacccg gatcatcgag ggcgagccgg agaccgacct agcgggcgat gacgccgaag actggtcgcg caccggcccg gccgagcgcg tcgacatcgc gatcgtgggc ttgtccaccc gcttcccggg cgagatgaac acccccgagc agacctggca ggcgctgctg gaaggccgcg acgggatcac cgacctgccc gacgggcgct ggtcggaatt cctcgaagag ccgcggctgg ccgcgcgggt cgccggggcc cgcacccggg gcggctacct gaaggacatc aagggcttcg attcggagtt cttcgcggtg gccaagaccg aagccgacaa catcgacccg cagcagcgga tggcgctgga gctgacctgg gaggcgctcg agcacgcccg catcccggcg tcgagcctgc gcggccaggc cgtcggtgtg tacatcggca gctccaccaa cgactacagc ttcctggcgg tgtcggaccc gacggtcgcg cacccgtatg cgatcaccgg caccagcagc tcgatcatcg ccaaccgggt gtcctacttc tacgacttcc acggaccgtc ggtcaccatt gacaccgcgt gctcgagttc gctggtggcc atccaccagg gggtgcaggc gctgcgcaac ggcgaggccg acgtagtggt cgccggcggg gtgaacgcgt tgatcacacc gatggtcacc ctgggtttcg acgagatcgg tgcggtgctg gcgcccgacg gccggatcaa gtcgttctca gccgacgccg acggctacac ccgctccgaa ggcggcggca tgctggtgct caagcgggtc gacgacgccc gccgcgacgg cgacgcgatc ctggccgtga tcgccggcag cgcggtcaac cacgacggcc ggtccaacgg cctgatcgca cccaaccagg acgcgcaggc cgacgtgctg cgccgggcct acaaggacgc cggcatcgat ccgcgcaccg tcgactacat cgaggcgcac ggcaccggca ccatcctcgg cgacccaatc gaggccgagg cgctgggccg ggtggtcggt aggggccgtc cggccgatcg gccggcgctg ctgggtgcgg tgaaaaccaa cgtcgggcac ctggaatcgg cggccggcgc ggccagcatg gccaaggtgg tgctggcgct gcagcacgac aaactgccgc cgtcgatcaa cttcgccggc cccagcccct acatcgactt cgacgcgatg cggttgaaga tgatcaccac gcccaccgac tggccgcgat acggcggcta cgcgctggcc ggggtgtcca gcttcggctt cggcggcgcc aacgcgcacg tggtggtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MytuD.20406.a.B7.GE43837
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 62kDa
OBSERVED MW: 62kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 84
PERCENT COVERAGE: 53
Validated AA Sequence
MAHHHHHHMS QENAPAERAE LTVPEMRQWL RNWVGKAVGK APDSIDESVP MVELGLSSRD AVAMAADIED LTGVTLSVAV AFAHPTIESL ATRIIEGEPE TDLAGDDAED WSRTGPAERV DIAIVGLSTR FPGEMNTPEQ TWQALLEGRD GITDLPDGRW SEFLEEPRLX ARVAGARTRG GYLXDIXGFD SXFFAVXXTE ADNIDPQQRM ALELTWXXLE HARIPASXLX GQAVXVYIGS SXXDYXFLAV SXXXVAHPYA ITGTXSSIIA XRXXYFYXXP RTVGXXXXRV LEFAXXXXXR GXXXCAXXXX X
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc caggagaacg cccccgccga aagggccgag ctaacggtcc ccgagatgcg ccagtggctg cgcaactggg tgggtaaggc cgtcggaaag gcaccggact cgatcgacga atcggtgccc atggtggagc tgggtctgtc gtcgcgcgat gccgtcgcga tggccgccga catagaagac ctgaccgggg tcacgctgtc ggtcgcggtg gcgttcgcgc atccgaccat cgaatcgctg gccacccgga tcatcgaggg cgagccggag accgacctag cgggcgatga cgccgaagac tggtcgcgca ccggcccggc cgagcgcgtc gacatcgcga tcgtgggctt gtccacccgc ttcccgggcg agatgaacac ccccgagcag acctggcagg cgctgctgga aggncgcgac gggatcaccg acctgcccga cgggcgctgg tcggaattcc tcgaagagcc gcggctggnc gcgcgggtcg ccggggcccg cacccggggc ggctacctga angacatcna gggcttcgat tcgganttct tcgcggtggn cnagaccgaa gccgacaaca tcgacccgca gcagcggatg gcgctggagc tgacctggga ngngctcgag cacgcccgca tcccggcgtc gagnctgngc ggncaggccg tcngtgtgta catcggcagc tccnccnacg actacngctt cctggcggtg tcggancnga nggtcgcgca cccgtatgcn atcaccggca ccnncagctc gatcatcgcn naccgggngn cctacttcta cnanntnccn cggaccgtcg gnnnncntng acnccgcgtg ctcgagttcg ctgnnnnncn anccnccagg gggnncannn gntgcgcnnc ggngagncng angtagtggt cgccggnggg gnnnancgcg ttgatcacac ngangntcac cctgggtttn gacgagatcg gtgcggtgct ggcgcccgac gnccggatca agtcnttntc anccgacgcc gacggntaca cccgntccga aggcggcggc atgctggtgc tcaagcggnt cgacgacgcc cgccgcgacg gcgacgcgat cctgnccgtg atcgccggca gcgcggtcaa ccacgacggc cgntccaacg ncctgatcgc acccaaccag gacgcgcagg ccgacgtgct gcgccgggcc tacaaggacg ccggcatcga tccgcgcacc gtcgactaca tcgaggcgca cggcaccggc accatcctcg gcgacccaat cgaggccgag gcgctgggcc gggtggtcgg taggggccgt ccggccgatc ggccggcgct gctgggtgcg gtgaaaacca acgtcgggca cctggaatcg gcggccggcg cggccagcat ggccaaggtg gtgctggcgc tgcagcacga caaactgccg ccgtcgatca acttcgccgg ccccagcccc tacatcgact tcgacgcgat gcggttgaag atgatcacca cgcccaccga ctggccgcga tacggcggct acgcgctggc cggggtgtcc agcttcggct tcggcggcgc caacgcgcac gtggtggtgc gcgaggtcct gccgcgtgac gtggtggaaa aggaaccgga acccgagccg gaacccaagg cggccgccga acccgccgag gcgcccacgt tggcaggcca cgc
Expected Protein Sequence
MAHHHHHHSQ ENAPAERAEL TVPEMRQWLR NWVGKAVGKA PDSIDESVPM VELGLSSRDA VAMAADIEDL TGVTLSVAVA FAHPTIESLA TRIIEGEPET DLAGDDAEDW SRTGPAERVD IAIVGLSTRF PGEMNTPEQT WQALLEGRDG ITDLPDGRWS EFLEEPRLAA RVAGARTRGG YLKDIKGFDS EFFAVAKTEA DNIDPQQRMA LELTWEALEH ARIPASSLRG QAVGVYIGSS TNDYSFLAVS DPTVAHPYAI TGTSSSIIAN RVSYFYDFHG PSVTIDTACS SSLVAIHQGV QALRNGEADV VVAGGVNALI TPMVTLGFDE IGAVLAPDGR IKSFSADADG YTRSEGGGML VLKRVDDARR DGDAILAVIA GSAVNHDGRS NGLIAPNQDA QADVLRRAYK DAGIDPRTVD YIEAHGTGTI LGDPIEAEAL GRVVGRGRPA DRPALLGAVK TNVGHLESAA GAASMAKVVL ALQHDKLPPS INFAGPSPYI DFDAMRLKMI TTPTDWPRYG GYALAGVSSF GFGGANAHVV VREVLPRDVV EKEPEPEPEP KAAAEPAEAP TLAGHALRFD EFGN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcccagg agaacgcccc cgccgaaagg gccgagctaa cggtccccga gatgcgccag tggctgcgca actgggtggg taaggccgtc ggaaaggcac cggactcgat cgacgaatcg gtgcccatgg tggagctggg tctgtcgtcg cgcgatgccg tcgcgatggc cgccgacata gaagacctga ccggggtcac gctgtcggtc gcggtggcgt tcgcgcatcc gaccatcgaa tcgctggcca cccggatcat cgagggcgag ccggagaccg acctagcggg cgatgacgcc gaagactggt cgcgcaccgg cccggccgag cgcgtcgaca tcgcgatcgt gggcttgtcc acccgcttcc cgggcgagat gaacaccccc gagcagacct ggcaggcgct gctggaaggc cgcgacggga tcaccgacct gcccgacggg cgctggtcgg aattcctcga agagccgcgg ctggccgcgc gggtcgccgg ggcccgcacc cggggcggct acctgaagga catcaagggc ttcgattcgg agttcttcgc ggtggccaag accgaagccg acaacatcga cccgcagcag cggatggcgc tggagctgac ctgggaggcg ctcgagcacg cccgcatccc ggcgtcgagc ctgcgcggcc aggccgtcgg tgtgtacatc ggcagctcca ccaacgacta cagcttcctg gcggtgtcgg acccgacggt cgcgcacccg tatgcgatca ccggcaccag cagctcgatc atcgccaacc gggtgtccta cttctacgac ttccacggac cgtcggtcac cattgacacc gcgtgctcga gttcgctggt ggccatccac cagggggtgc aggcgctgcg caacggcgag gccgacgtag tggtcgccgg cggggtgaac gcgttgatca caccgatggt caccctgggt ttcgacgaga tcggtgcggt gctggcgccc gacggccgga tcaagtcgtt ctcagccgac gccgacggct acacccgctc cgaaggcggc ggcatgctgg tgctcaagcg ggtcgacgac gcccgccgcg acggcgacgc gatcctggcc gtgatcgccg gcagcgcggt caaccacgac ggccggtcca acggcctgat cgcacccaac caggacgcgc aggccgacgt gctgcgccgg gcctacaagg acgccggcat cgatccgcgc accgtcgact acatcgaggc gcacggcacc ggcaccatcc tcggcgaccc aatcgaggcc gaggcgctgg gccgggtggt cggtaggggc cgtccggccg atcggccggc gctgctgggt gcggtgaaaa ccaacgtcgg gcacctggaa tcggcggccg gcgcggccag catggccaag gtggtgctgg cgctgcagca cgacaaactg ccgccgtcga tcaacttcgc cggccccagc ccctacatcg acttcgacgc gatgcggttg aagatgatca ccacgcccac cgactggccg cgatacggcg gctacgcgct ggccggggtg tccagcttcg gcttcggcgg cgccaacgcg cacgtggtgg tgcgcgaggt cctgccgcgt gacgtggtgg aaaaggaacc ggaacccgag ccggaaccca aggcggccgc cgaacccgcc gaggcgccca cgttggcagg ccacgcgctg cggttcgacg agttcggcaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat