MytuD.20386.a

Uncharacterized protein; EspM

CENTER ID: MytuD.20386.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.20386.a.B1.GE43633 full length 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.111.peg.4299
TubercuList: Rv3863
MTB Network Portal: Rv3863
UniProt: P96214

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGERKVCPP SRLVPANKGS TQMSKAGSTV GPAPLVACSG GTSDVIEPRR GVAIIGHSCR VGTQIDDSRI SQTHLRAVSD DGRWRIVGNI PRGMFVGGRR GSSVTVSDKT LIRFGDPPGG KALTFEVVRP SDSAAQHGRV QPSADLSDDP AHNAAPVAPD PGVVRAGAAA AARRRELDIS QRSLAADGII NAGALIAFEK GRSWPRERTR AKLEEVLQWP AGTIARIRRG EPTEPATNPD ASPGLRPADG PASLIAQAVT AAVDGCSLAI AALPATEDPE FTERAAPILA DLRQLEAIAV QATRISRITP ELIKALGAVR RHHDELMRLG ATAPGATLAQ RLYAARRRAN LSTLETAQAA GVAEEMIVGA EAEEELPAEA TEAIEALIRQ IN
NT Sequence
atggcgggcg agcggaaagt ctgcccaccg tcccggctag tacccgcgaa taagggatca acgcagatgt ctaaagcagg gtcgactgtc ggaccggcgc cgctggtcgc gtgcagcggc ggcacatcag acgtgattga gccccgtcgc ggtgtcgcga tcattggcca ctcgtgccga gtcggcaccc agatcgacga ttctcgaatc tctcagacac atctgcgagc ggtatccgat gatggacggt ggcggatcgt cggcaacatc ccgagaggta tgttcgtcgg cggacgacgc ggcagctcgg tgaccgtcag cgataagacc ctaatccgat tcggcgatcc ccctggaggc aaggcgttga cgttcgaagt cgtcaggccg tcggattccg ctgcacagca cggccgcgta caaccatcag cggacctgtc ggacgacccg gcgcacaacg ctgcgccggt cgcaccggac cccggcgtgg ttcgcgcagg ggcggccgcg gctgcgcgcc gtcgtgaact tgacatcagc caacgcagct tggcggccga cgggatcatc aacgcgggcg cgctcatcgc gttcgagaaa ggccgtagtt ggccccggga acggacccgg gcaaaactcg aagaagtgct gcagtggccc gctggaacca tcgcgcgaat ccgtcggggc gagcccaccg agcccgcaac aaaccccgac gcgtcccccg gactccggcc tgccgacggc ccggcgtcct tgatcgcgca ggctgtcacc gccgccgtag acggctgcag tctggctatc gcagcgttgc cggcgaccga ggaccccgag ttcaccgaac gtgccgcgcc gatccttgct gatttgcgcc agctcgaggc gattgccgtc caagcaaccc gcatcagccg gattaccccg gaattgatca aggcgttggg cgcggtacgt cgccaccacg acgaattaat gaggctggga gcaaccgccc ctggtgccac actggcgcag cgcttatatg ccgcacggcg gcgcgcgaac ctttccaccc tggagactgc ccaagcggcc ggcgtcgcag aagaaatgat cgtcggcgcc gaagccgagg aagagttgcc agccgaggcc accgaagcga tcgaagcact gatccgtcag atcaattga
Details for MytuD.20386.a.B1.GE43633
HARVESTED ON: 2/3/2020
SEQUENCED ON: 2/13/2020
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 95
PERCENT COVERAGE: 45
Validated AA Sequence
MAHHHHHHMA GERKVCPPSR LVPANKGSTQ MSKAGSTVGP APLVACSGGT SDVIEPRRGV AIIGHSCRVG TQIDDSRISQ THLRAXSXDG RWRIVGNIPR GMFVGGRRGS SVTVSDKTLI RFGDPPGGKA LTFEVVRPSD SAAQXXRVQP SXDLSDDPAH NAAPVAPXPG VVRAXXAAAA X
Validated NT Sequence
atggctcacc accaccacca ccatatggcg ggcgagcgga aagtctgccc accgtcccgg ctagtacccg cgaataaggg atcaacgcag atgtctaaag cagggtcgac tgtcggaccg gcgccgctgg tcgcgtgcag cggcggcaca tcagacgtga ttgagccccg tcgcggtgtc gcgatcattg gccactcgtg ccgagtcggc acccagatcg acgattctcg aatctctcag acncatctgc gagcggnatc cnatgatgga cggtggcgga tcgtcggcaa catcccgaga ggtatgttcg tcggcggacg acgcggcagc tcggtgaccg tcagcgataa gaccctaatc cgattcggcg atccccctgg aggcaaggcg ttgacgttcg aagtcgtcag gccgtcngat tccgctgcac agnacngncg cgtacaacca tcancggacc tgtcggacga cccggcgcac aacgctgcgc cggtcgcacc gnnncccggc gtggttcgcg cangggnggc cgcggctgcn ngccnt
Expected Protein Sequence
MAHHHHHHMA GERKVCPPSR LVPANKGSTQ MSKAGSTVGP APLVACSGGT SDVIEPRRGV AIIGHSCRVG TQIDDSRISQ THLRAVSDDG RWRIVGNIPR GMFVGGRRGS SVTVSDKTLI RFGDPPGGKA LTFEVVRPSD SAAQHGRVQP SADLSDDPAH NAAPVAPDPG VVRAGAAAAA RRRELDISQR SLAADGIINA GALIAFEKGR SWPRERTRAK LEEVLQWPAG TIARIRRGEP TEPATNPDAS PGLRPADGPA SLIAQAVTAA VDGCSLAIAA LPATEDPEFT ERAAPILADL RQLEAIAVQA TRISRITPEL IKALGAVRRH HDELMRLGAT APGATLAQRL YAARRRANLS TLETAQAAGV AEEMIVGAEA EEELPAEATE AIEALIRQIN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg gcgagcggaa agtctgccca ccgtcccggc tagtacccgc gaataaggga tcaacgcaga tgtctaaagc agggtcgact gtcggaccgg cgccgctggt cgcgtgcagc ggcggcacat cagacgtgat tgagccccgt cgcggtgtcg cgatcattgg ccactcgtgc cgagtcggca cccagatcga cgattctcga atctctcaga cacatctgcg agcggtatcc gatgatggac ggtggcggat cgtcggcaac atcccgagag gtatgttcgt cggcggacga cgcggcagct cggtgaccgt cagcgataag accctaatcc gattcggcga tccccctgga ggcaaggcgt tgacgttcga agtcgtcagg ccgtcggatt ccgctgcaca gcacggccgc gtacaaccat cagcggacct gtcggacgac ccggcgcaca acgctgcgcc ggtcgcaccg gaccccggcg tggttcgcgc aggggcggcc gcggctgcgc gccgtcgtga acttgacatc agccaacgca gcttggcggc cgacgggatc atcaacgcgg gcgcgctcat cgcgttcgag aaaggccgta gttggccccg ggaacggacc cgggcaaaac tcgaagaagt gctgcagtgg cccgctggaa ccatcgcgcg aatccgtcgg ggcgagccca ccgagcccgc aacaaacccc gacgcgtccc ccggactccg gcctgccgac ggcccggcgt ccttgatcgc gcaggctgtc accgccgccg tagacggctg cagtctggct atcgcagcgt tgccggcgac cgaggacccc gagttcaccg aacgtgccgc gccgatcctt gctgatttgc gccagctcga ggcgattgcc gtccaagcaa cccgcatcag ccggattacc ccggaattga tcaaggcgtt gggcgcggta cgtcgccacc acgacgaatt aatgaggctg ggagcaaccg cccctggtgc cacactggcg cagcgcttat atgccgcacg gcggcgcgcg aacctttcca ccctggagac tgcccaagcg gccggcgtcg cagaagaaat gatcgtcggc gccgaagccg aggaagagtt gccagccgag gccaccgaag cgatcgaagc actgatccgt cagatcaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat