MytuD.20375.a

Uncharacterized protein (Rv1376)

CENTER ID: MytuD.20375.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.20375.a.B1.GE43507 full length 1 497
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.111.peg.1535
TubercuList: Rv1376
MTB Network Portal: Rv1376
UniProt: P71804

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTACGRIVVT AGPTISAADI RSVVPDAEVA PPIAFGQALS YDLRSGDTLL IVDGLFFQQP SVRHKELLTL MADGVRVVGS SSMGALRAAE LHPFGMEGYG WVFESYRDGV LEADDEVGVV HGDADDGYPV FVDALVNMRH TLARAVATGV VCSELAERII ETARATPFTM RTWARLLSEV GAPDQRGLAA QLRSLRVDVK HADALLALRQ LGQRPRVEPL RPGPPPTVWS RRWRQRWAPP TSVAASADHG ESFVDVTDLE VLSFLSVSSV DYWAYRPALQ QVAAWYWTLK HPEQSGSVGE RAARAVAEVA SEGYGRALEF IAYRYALATG IIDETGFPEA VAAHWLTTEE RHGLGNDPIS ISARVITRTL FVVRLLPAID HFLDLLRKDS RLPRWRAMAA HALCKRDDLA RQKPHLNLGR PDPTQLKRLF GARWGTQVNR IELARRGLMT EDAFYAAATP FAVAAVDDQL PRIEVGTLGP APLSADVPER HFDFGSV
NT Sequence
atgacggcct gcggcaggat tgtcgtcacc gctgggccca cgattagcgc cgcggacatc cgctcggtgg tgccggatgc cgaggtggcg ccgccgattg cgtttggcca ggcgctctcc tatgacttgc ggtcgggtga cacgctgctg attgtcgacg gattgttctt tcagcagccg tcggttcgac ataaggagct tttgacgttg atggccgacg gtgtccgagt cgtcggatcg tcgagcatgg gcgccctgcg ggccgctgag ctgcatccat tcggcatgga gggctatggc tgggtcttcg aaagctaccg agatggggta ctcgaggccg acgatgaggt cggcgtggtg cacggcgacg ccgacgacgg ctacccggtc ttcgtcgacg cgctggtgaa catgcgccac accctggcgc gggccgtcgc aactggtgtg gtgtgctccg agctggccga gcggatcatc gagaccgcgc gggccacacc gttcaccatg cgcacctggg cgcggctgct gagtgaggtc ggcgccccgg accagcgcgg cctcgccgca cagttgcggt cactgcgggt cgatgtcaaa cacgccgatg cgctgctggc gttgcggcag ctcggccagc gcccccgggt ggagccgctt cgtccgggtc cgccgcccac cgtgtggtcg cggcggtggc ggcagcgatg ggcaccgccc acctccgtcg ccgcatcggc cgaccacggc gagtcttttg tcgacgtcac cgacttggag gtcttgtcgt ttttgagcgt gagctcggtt gactactggg cctaccggcc agcactgcaa caggtcgctg cctggtactg gacgttgaaa caccccgaac aatccggaag cgtcggtgag cgtgccgcac gagccgtcgc cgaggtggca tcggagggct acgggcgcgc cctggaattc attgcctatc gctacgcact tgccaccggc atcatcgacg agaccggctt tcccgaggcg gtcgcagcgc attggctcac caccgaagag cgccacggcc tgggcaatga ccccatctcg atctcggcgc gagtgatcac ccgcacgttg ttcgtcgtcc ggttattgcc ggcgatcgac catttccttg acctgctgcg gaaggactcc cgactgcccc gatggcgtgc catggcggcc cacgcactct gcaagcgcga cgatctggcc cggcaaaagc cgcacctgaa cctgggccgg cccgatccga cgcaattgaa gcgcctcttt ggggcccgat gggggaccca ggtgaaccgc atcgagttgg cccggcgtgg actgatgacc gaggacgcct tctatgctgc cgccaccccg ttcgccgtcg cggccgtcga cgaccaactg ccgcgcatcg aggtcggcac cttaggaccc gcgccgctga gcgcggacgt tccagaacgc catttcgact tcggttccgt ctaa
Details for MytuD.20375.a.B1.GE43507
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMT ACGRIVVTAG PTISAADIRS VVPDAEVAPP IAFGQALSYD LRSGDTLLIV DGLFFQQPSV RHKELLTLMA DGVRVVGSSS MGALRAAELH PFGMEGYGWV FESYRDGVLE ADDEVGVVHG DADDGYPVFV DALVNMRHTL ARAVATGVVC SELAERIIET ARATPFTMRT WARLLSEVGA PDQRGLAAQL RSLRVDVKHA DALLALRQLG QRPRVEPLRP GPPPTVWSRR WRQRWAPPTS VAASADHGES FVDVTDLEVL SFLSVSSVDY WAYRPALQQV AAWYWTLKHP EQSGSVGERA ARAVAEVASE GYGRALEFIA YRYALATGII DETGFPEAVA AHWLTTEERH GLGNDPISIS ARVITRTLFV VRLLPAIDHF LDLLRKDSRL PRWRAMAAHA LCKRDDLARQ KPHLNLGRPD PTQLKRLFGA RWGTQVNRIE LARRGLMTED AFYAAATPFA VAAVDDQLPR IEVGTLGPAP LSADVPERH
Validated NT Sequence
gaaatggcgt tctggaacgt ccgcgctcag cggcgcgggt cctaaggtgc cgacctcgat gcgcggcagt tggtcgtcga cggccgcgac ggcgaacggg gtggcggcag catagaaggc gtcctcggtc atcagtccac gccgggccaa ctcgatgcgg ttcacctggg tcccccatcg ggccccaaag aggcgcttca attgcgtcgg atcgggccgg cccaggttca ggtgcggctt ttgccgggcc agatcgtcgc gcttgcagag tgcgtgggcc gccatggcac gccatcgggg cagtcgggag tccttccgca gcaggtcaag gaaatggtcg atcgccggca ataaccggac gacgaacaac gtgcgggtga tcactcgcgc cgagatcgag atggggtcat tgcccaggcc gtggcgctct tcggtggtga gccaatgcgc tgcgaccgcc tcgggaaagc cggtctcgtc gatgatgccg gtggcaagtg cgtagcgata ggcaatgaat tccagggcgc gcccgtagcc ctccgatgcc acctcggcga cggctcgtgc ggcacgctca ccgacgcttc cggattgttc ggggtgtttc aacgtccagt accaggcagc gacctgttgc agtgctggcc ggtaggccca gtagtcaacc gagctcacgc tcaaaaacga caagacctcc aagtcggtga cgtcgacaaa agactcgccg tggtcggccg atgcggcgac ggaggtgggc ggtgcccatc gctgccgcca ccgccgcgac cacacggtgg gcggcggacc cggacgaagc ggctccaccc gggggcgctg gccgagctgc cgcaacgcca gcagcgcatc ggcgtgtttg acatcgaccc gcagtgaccg caactgtgcg gcgaggccgc gctggtccgg ggcgccgacc tcactcagca gccgcgccca ggtgcgcatg gtgaacggtg tggcccgcgc ggtctcgatg atccgctcgg ccagctcgga gcacaccaca ccagttgcga cggcccgcgc cagggtgtgg cgcatgttca ccagcgcgtc gacgaagacc gggtagccgt cgtcggcgtc gccgtgcacc acgccgacct catcgtcggc ctcgagtacc ccatctcggt agctttcgaa gacccagcca tagccctcca tgccgaatgg atgcagctca gcggcccgca gggcgcccat gctcgacgat ccgacgactc ggacaccgtc ggccatcaac gtcaaaagct ccttatgtcg aaccgacggc tgctgaaaga acaatccgtc gacaatcagc agcgtgtcac ccgaccgcaa gtcataggag agcgcctggc caaacgcaat cggcggcgcc acctcggcat ccggcaccac cgagcggatg tccgcggcgc taatcgtggg cccagcggtg acgacaatcc tgccgcaggc cgtcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMT ACGRIVVTAG PTISAADIRS VVPDAEVAPP IAFGQALSYD LRSGDTLLIV DGLFFQQPSV RHKELLTLMA DGVRVVGSSS MGALRAAELH PFGMEGYGWV FESYRDGVLE ADDEVGVVHG DADDGYPVFV DALVNMRHTL ARAVATGVVC SELAERIIET ARATPFTMRT WARLLSEVGA PDQRGLAAQL RSLRVDVKHA DALLALRQLG QRPRVEPLRP GPPPTVWSRR WRQRWAPPTS VAASADHGES FVDVTDLEVL SFLSVSSVDY WAYRPALQQV AAWYWTLKHP EQSGSVGERA ARAVAEVASE GYGRALEFIA YRYALATGII DETGFPEAVA AHWLTTEERH GLGNDPISIS ARVITRTLFV VRLLPAIDHF LDLLRKDSRL PRWRAMAAHA LCKRDDLARQ KPHLNLGRPD PTQLKRLFGA RWGTQVNRIE LARRGLMTED AFYAAATPFA VAAVDDQLPR IEVGTLGPAP LSADVPERHF DFGSV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg cctgcggcag gattgtcgtc accgctgggc ccacgattag cgccgcggac atccgctcgg tggtgccgga tgccgaggtg gcgccgccga ttgcgtttgg ccaggcgctc tcctatgact tgcggtcggg tgacacgctg ctgattgtcg acggattgtt ctttcagcag ccgtcggttc gacataagga gcttttgacg ttgatggccg acggtgtccg agtcgtcgga tcgtcgagca tgggcgccct gcgggccgct gagctgcatc cattcggcat ggagggctat ggctgggtct tcgaaagcta ccgagatggg gtactcgagg ccgacgatga ggtcggcgtg gtgcacggcg acgccgacga cggctacccg gtcttcgtcg acgcgctggt gaacatgcgc cacaccctgg cgcgggccgt cgcaactggt gtggtgtgct ccgagctggc cgagcggatc atcgagaccg cgcgggccac accgttcacc atgcgcacct gggcgcggct gctgagtgag gtcggcgccc cggaccagcg cggcctcgcc gcacagttgc ggtcactgcg ggtcgatgtc aaacacgccg atgcgctgct ggcgttgcgg cagctcggcc agcgcccccg ggtggagccg cttcgtccgg gtccgccgcc caccgtgtgg tcgcggcggt ggcggcagcg atgggcaccg cccacctccg tcgccgcatc ggccgaccac ggcgagtctt ttgtcgacgt caccgacttg gaggtcttgt cgtttttgag cgtgagctcg gttgactact gggcctaccg gccagcactg caacaggtcg ctgcctggta ctggacgttg aaacaccccg aacaatccgg aagcgtcggt gagcgtgccg cacgagccgt cgccgaggtg gcatcggagg gctacgggcg cgccctggaa ttcattgcct atcgctacgc acttgccacc ggcatcatcg acgagaccgg ctttcccgag gcggtcgcag cgcattggct caccaccgaa gagcgccacg gcctgggcaa tgaccccatc tcgatctcgg cgcgagtgat cacccgcacg ttgttcgtcg tccggttatt gccggcgatc gaccatttcc ttgacctgct gcggaaggac tcccgactgc cccgatggcg tgccatggcg gcccacgcac tctgcaagcg cgacgatctg gcccggcaaa agccgcacct gaacctgggc cggcccgatc cgacgcaatt gaagcgcctc tttggggccc gatgggggac ccaggtgaac cgcatcgagt tggcccggcg tggactgatg accgaggacg ccttctatgc tgccgccacc ccgttcgccg tcgcggccgt cgacgaccaa ctgccgcgca tcgaggtcgg caccttagga cccgcgccgc tgagcgcgga cgttccagaa cgccatttcg acttcggttc cgtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat