MytuD.20304.a

Possible bifunctional enzyme long-chain acyl-CoA synthase and lipase (Rv1683)

CENTER ID: MytuD.20304.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.20304.a.B2.GE43502 lipase domain (longest) 1 369
MytuD.20304.a.B3.GE43503 lipase domain (med) 73 345
MytuD.20304.a.B4.GE43504 acetyl coA synthetase domain 443 960
MytuD.20304.a.B5.GE43505 lipase domain (short) 41 360
MytuD.20304.a.HZ1.GE43745 full length 1 999
MytuD.20304.a.HZ2.GE43746 lipase domain (longest) 1 369
MytuD.20304.a.HZ5.GE43748 lipase domain (short) 41 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.111.peg.1870
TubercuList: Rv1683
MTB Network Portal: Rv1683
UniProt: O33185

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVDLNFSMVT RPIERLVATA QNGLEVLRLG GLETGSVPSP SQIVESVPMY KLRRYFPPDN RPGQPPVGPP VLMVHPMMMS ADMWDVTRED GAVGILHASG LDPWVIDFGS PDEVEGGMRR NLADHIVALS EAVDTVKDAT GHDVHFVGYS QGGMFCYQAA AYRRSKDIAS VVAFGSPVDT LAALPMGIPA NMGAAVADFM ADHVFNRLDI PSWMARMGFQ MMDPLKTAKA RVDFVRQLHD REALLPREQQ RRFLESEGWI AWSGPAISEL LKQFIAHNRM MTGGFAISGQ MVTLTDITCP ILAFVGEVDD IGQPASVRGI RRAAPNSEVY ECLIRAGHFG LVVGSRAAQQ SWPTVADWVR WISGDGTKPE NIHLMADQPA EHTDSGVAFS SRVAHGIGEV SEAALALARG AADAVVAANR SVRTLAVETV RTLPRLARLG QLNDHTRISL GRIIDEQAHD APKGEFLLFD GRVHTYEAVN RRINNVVRGL IAVGVRQGDR VGVLMETRPS ALVAIAALSR LGAVAVVMRP DTDLSASVRL GRVTEILTDP TNLDAARQLP GQVLVLGGGE SRDLDLPADA LEQGQVIDME KIDPDAVELP AWYRPNPGLA RDLAFIAFSS ADGDLVAKQI TNYRWAVSAF GTASTAALGR RDTVYCLTPL HHESALLVSL GGAVVGGTRI ALSRGLRPDR FVAEVRQYGV TVVSYTWAML RDVVDDPAFV LHGNHPVRLF IGSGMPTGLW ERVVEAFAPA HVVEFFATTD GQAVLANVAG AKIGSKGRPL PGAGRVELGA YDAEHDLILE NDRGFVQVAG VNQVGVLLAQ SRGPIDPTAS VKRGVFAPAD TWISTDYLFW RDDDGDYWLA GGRGSVVRTA RGMVYTEPVT NALGLITGVD LAVTYGVLVR GRHVAVSAVT LLPGATITAA DLTEAVASMP VGLGPDIVHV VPQLTLSGTY RPTVSALRAN GIPKAGRQAW YFNSGGNEYR RLTPAVRTEL TGQHRRGNA
NT Sequence
gtggtggatc tcaatttttc gatggtcacg cgaccaatcg agcgcctggt ggccacggcg cagaacggtc tggaagtcct gcgactcggg ggcctggaaa ccggcagtgt tccgtcgccg tcccaaatcg ttgagagcgt accgatgtac aagctgcggc ggtattttcc gccggacaac cgcccgggac agccaccggt gggtccgccg gtgctgatgg tgcacccgat gatgatgtcg gcggacatgt gggacgtcac ccgtgaagac ggcgcggtgg ggatcctgca cgccagcggg ctagatccct gggtcatcga cttcggctca cccgacgagg tcgagggcgg aatgcgccgt aacctggccg accacatcgt cgccctcagc gaggcggtcg ataccgtcaa ggacgccact ggccacgatg tgcacttcgt cgggtattcg cagggtggca tgttctgcta tcaggccgcg gcataccggc gttcgaagga catcgccagc gtggtcgcgt tcggctcgcc ggtggacacc ctggccgcgt tgcccatggg catcccggcg aacatgggcg ctgcggtcgc cgatttcatg gccgatcacg tcttcaatcg cttggatatc ccaagctgga tggcgcgcat gggttttcag atgatggacc cactcaaaac cgcgaaggcc cgggtggact tcgtgcgtca gttgcacgac cgcgaggcac tgctgccgcg ggaacaacag cgccggttcc tggaatccga aggatggatc gcctggtcgg gcccggcgat ctcggaactg ctcaagcagt tcatcgcgca caaccgaatg atgacgggtg gtttcgccat cagcggccag atggtgacgc ttaccgatat cacttgcccg atactggcgt tcgtcggtga ggtcgacgac atcggccagc cggcgtcggt acgcggcatc cggcgggccg cgcccaactc cgaggtctac gaatgtctca tccgggcagg gcatttcggt ctcgtcgtgg gatcccgagc ggcacaacag agctggccga ccgtggccga ctgggtgcgc tggatctccg gcgacggcac caaaccggaa aacatccacc tgatggccga tcagccggcc gaacacaccg atagcggtgt ggctttcagc tcccgggtcg cgcacggcat cggggaggtc tcggaggctg cgttggcgct ggctcgcggc gcggccgacg cggtcgttgc ggccaacaga tcggtgcgca cgctggcggt ggagacggtg cggacgctgc cgcgactagc ccggttgggt cagctcaacg accacacccg gatctcgctg ggccgcatca tcgacgaaca ggcacacgat gccccgaagg gtgaattcct gttgttcgac gggcgcgtgc acacctatga ggcggtaaac cggcggatca acaatgtcgt tcgtggcctc atcgcggtcg gggtgcggca gggtgaccgt gtcggcgtgc tgatggagac tcggcccagc gcgctggtcg ccatcgccgc gctgtctcgg ctgggagcgg ttgccgtggt gatgcggcca gacaccgacc tgtccgcgtc ggtccggctc gggagagtga ccgagatcct gaccgaccct accaatctgg atgctgcgcg ccagttgccc ggacaggtgc tggtgttggg tggtggtgaa tcgcgtgatc tggatctgcc ggccgacgca cttgaacagg gccaagtcat cgacatggaa aaaatcgacc cggacgccgt cgagttgccg gcgtggtatc gaccgaatcc cggattggcg cgggatctgg cgttcatcgc gttcagttcg gccgacggcg acctggtggc caagcagatc accaactacc gctgggcggt gtcggccttc gggaccgcct cgacggcggc cctcggccgc agagacacgg tgtactgttt gacgccgctg caccatgagt ccgcactgtt ggtcagcctg ggcggcgcgg tcgtgggcgg aacccgtatc gcattgtccc gcggcttgcg cccggaccgg ttcgtggccg aggtacgcca gtacggcgtc accgtcgtct cctacacatg ggccatgctg cgtgacgtgg tcgacgatcc ggcgttcgtg ttgcacggca accatccggt gcggttgttc atcggctcgg gcatgccgac cggattgtgg gagcgggtcg tcgaagcgtt cgcaccggcg cacgtcgtcg agtttttcgc caccaccgac ggacaggcgg tgctggccaa cgtggctggc gccaagatcg gcagcaaggg ccgtccgttg cctggcgccg gacgtgtcga acttggggcc tacgacgccg aacatgacct gatcctggag aacgaccgcg gcttcgtgca ggtcgccggt gtcaaccagg tcggggtgct gctcgcacaa tccagagggc cgatcgatcc gaccgcgtcg gtcaaacgcg gtgtcttcgc tcccgccgac acctggatat ctaccgacta cctattctgg cgtgacgacg atggggacta ctggctggcg ggtggacgcg gctcggtggt gcgcactgcg cgcgggatgg tttacaccga gccggtcacc aacgcgttgg gcctcatcac cggtgtcgac ctcgcggtga cctacggtgt attggtgcgc ggtcgccacg tcgcggtgtc ggcggtgacg ttgctgcctg gagcgaccat cacagccgcc gacttgaccg aagccgtggc gagcatgccg gtggggctgg gacctgacat cgtgcacgtg gtgccgcagc taacgctcag cggtacttac cggccaacgg tcagcgcgtt gcgggccaac gggattccca aggcgggccg tcaggcatgg tatttcaact ccggcggcaa cgagtaccgg cggttgacgc cggcggtccg caccgagttg accggccagc atcggcgcgg caatgcttga
Details for MytuD.20304.a.B2.GE43502
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV VDLNFSMVTR PIERLVATAQ NGLEVLRLGG LETGSVPSPS QIVESVPMYK LRRYFPPDNR PGQPPVGPPV LMVHPMMMSA DMWDVTREDG AVGILHASGL DPWVIDFGSP DEVEGGMRRN LADHIVALSE AVDTVKDATG HDVHFVGYSQ GGMFCYQAAA YRRSKDIASV VAFGSPVDTL AALPMGIPAN MGAAVADFMA DHVFNRLDIP SWMARMGFQM MDPLKTAKAR VDFVRQLHDR EALLPREQQR RFLESEGWIA WSGPAISELL KQFIAHNRMM TGGFAISGQM VTLTDITCPI LAFVGEVDDI GQPASVRGIR RAAPNSEVYE CLIRAGHFGL VVGSRAAQQS WPTVADWVRW IS
Validated NT Sequence
gccggagatc cagcgcaccc agtcggccac ggtcggccag ctctgttgtg ccgctcggga tcccacgacg agaccgaaat gccctgcccg gatgagacat tcgtagacct cggagttggg cgcggcccgc cggatgccgc gtaccgacgc cggctggccg atgtcgtcga cctcaccgac gaacgccagt atcgggcaag tgatatcggt aagcgtcacc atctggccgc tgatggcgaa accacccgtc atcattcggt tgtgcgcgat gaactgcttg agcagttccg agatcgccgg gcccgaccag gcgatccatc cttcggattc caggaaccgg cgctgttgtt cccgcggcag cagtgcctcg cggtcgtgca actgacgcac gaagtccacc cgggccttcg cggttttgag tgggtccatc atctgaaaac ccatgcgcgc catccagctt gggatatcca agcgattgaa gacgtgatcg gccatgaaat cggcgaccgc agcgcccatg ttcgccggga tgcccatggg caacgcggcc agggtgtcca ccggcgagcc gaacgcgacc acgctggcga tgtccttcga acgccggtat gccgcggcct gatagcagaa catgccaccc tgcgaatacc cgacgaagtg cacatcgtgg ccagtggcgt ccttgacggt atcgaccgcc tcgctgaggg cgacgatgtg gtcggccagg ttacggcgca ttccgccctc gacctcgtcg ggtgagccga agtcgatgac ccagggatct agcccgctgg cgtgcaggat ccccaccgcg ccgtcttcac gggtgacgtc ccacatgtcc gccgacatca tcatcgggtg caccatcagc accggcggac ccaccggtgg ctgtcccggg cggttgtccg gcggaaaata ccgccgcagc ttgtacatcg gtacgctctc aacgatttgg gacggcgacg gaacactgcc ggtttccagg cccccgagtc gcaggacttc cagaccgttc tgcgccgtgg ccaccaggcg ctcgattggt cgcgtgacca tcgaaaaatt gagatccacc accatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMV DLNFSMVTRP IERLVATAQN GLEVLRLGGL ETGSVPSPSQ IVESVPMYKL RRYFPPDNRP GQPPVGPPVL MVHPMMMSAD MWDVTREDGA VGILHASGLD PWVIDFGSPD EVEGGMRRNL ADHIVALSEA VDTVKDATGH DVHFVGYSQG GMFCYQAAAY RRSKDIASVV AFGSPVDTLA ALPMGIPANM GAAVADFMAD HVFNRLDIPS WMARMGFQMM DPLKTAKARV DFVRQLHDRE ALLPREQQRR FLESEGWIAW SGPAISELLK QFIAHNRMMT GGFAISGQMV TLTDITCPIL AFVGEVDDIG QPASVRGIRR AAPNSEVYEC LIRAGHFGLV VGSRAAQQSW PTVADWVRWI SGDGTKP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggtgg atctcaattt ttcgatggtc acgcgaccaa tcgagcgcct ggtggccacg gcgcagaacg gtctggaagt cctgcgactc gggggcctgg aaaccggcag tgttccgtcg ccgtcccaaa tcgttgagag cgtaccgatg tacaagctgc ggcggtattt tccgccggac aaccgcccgg gacagccacc ggtgggtccg ccggtgctga tggtgcaccc gatgatgatg tcggcggaca tgtgggacgt cacccgtgaa gacggcgcgg tggggatcct gcacgccagc gggctagatc cctgggtcat cgacttcggc tcacccgacg aggtcgaggg cggaatgcgc cgtaacctgg ccgaccacat cgtcgccctc agcgaggcgg tcgataccgt caaggacgcc actggccacg atgtgcactt cgtcgggtat tcgcagggtg gcatgttctg ctatcaggcc gcggcatacc ggcgttcgaa ggacatcgcc agcgtggtcg cgttcggctc gccggtggac accctggccg cgttgcccat gggcatcccg gcgaacatgg gcgctgcggt cgccgatttc atggccgatc acgtcttcaa tcgcttggat atcccaagct ggatggcgcg catgggtttt cagatgatgg acccactcaa aaccgcgaag gcccgggtgg acttcgtgcg tcagttgcac gaccgcgagg cactgctgcc gcgggaacaa cagcgccggt tcctggaatc cgaaggatgg atcgcctggt cgggcccggc gatctcggaa ctgctcaagc agttcatcgc gcacaaccga atgatgacgg gtggtttcgc catcagcggc cagatggtga cgcttaccga tatcacttgc ccgatactgg cgttcgtcgg tgaggtcgac gacatcggcc agccggcgtc ggtacgcggc atccggcggg ccgcgcccaa ctccgaggtc tacgaatgtc tcatccgggc agggcatttc ggtctcgtcg tgggatcccg agcggcacaa cagagctggc cgaccgtggc cgactgggtg cgctggatct ccggcgacgg caccaaaccg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MytuD.20304.a.B3.GE43503
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV HPMMMSADMW DVTREDGAVG ILHASGLDPW VIDFGSPDEV EGGMRRNLAD HIVALSEAVD TVKDATGHDV HFVGYSQGGM FCYQAAAYRR SKDIASVVAF GSPVDTLAAL PMGIPANMGA AVADFMADHV FNRLDIPSWM ARMGFQMMDP LKTAKARVDF VRQLHDREAL LPREQQRRFL ESEGWIAWSG PAISELLKQF IAHNRMMTGG FAISGQMVTL TDITCPILAF VGEVDDIGQP ASVRGIRRAA PNSEVYECLI RAGHFGLVVG S
Validated NT Sequence
atcttactca cttaggatcc cacgacgaga ccgaaatgcc ctgcccggat gagacattcg tagacctcgg agttgggcgc ggcccgccgg atgccgcgta ccgacgccgg ctggccgatg tcgtcgacct caccgacgaa cgccagtatc gggcaagtga tatcggtaag cgtcaccatc tggccgctga tggcgaaacc acccgtcatc attcggttgt gcgcgatgaa ctgcttgagc agttccgaga tcgccgggcc cgaccaggcg atccatcctt cggattccag gaaccggcgc tgttgttccc gcggcagcag tgcctcgcgg tcgtgcaact gacgcacgaa gtccacccgg gccttcgcgg ttttgagtgg gtccatcatc tgaaaaccca tgcgcgccat ccagcttggg atatccaagc gattgaagac gtgatcggcc atgaaatcgg cgaccgcagc gcccatgttc gccgggatgc ccatgggcaa cgcggccagg gtgtccaccg gcgagccgaa cgcgaccacg ctggcgatgt ccttcgaacg ccggtatgcc gcggcctgat agcagaacat gccaccctgc gaatacccga cgaagtgcac atcgtggcca gtggcgtcct tgacggtatc gaccgcctcg ctgagggcga cgatgtggtc ggccaggtta cggcgcattc cgccctcgac ctcgtcgggt gagccgaagt cgatgaccca gggatctagc ccgctggcgt gcaggatccc caccgcgccg tcttcacggg tgacgtccca catgtccgcc gacatcatca tcgggtgcac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMV HPMMMSADMW DVTREDGAVG ILHASGLDPW VIDFGSPDEV EGGMRRNLAD HIVALSEAVD TVKDATGHDV HFVGYSQGGM FCYQAAAYRR SKDIASVVAF GSPVDTLAAL PMGIPANMGA AVADFMADHV FNRLDIPSWM ARMGFQMMDP LKTAKARVDF VRQLHDREAL LPREQQRRFL ESEGWIAWSG PAISELLKQF IAHNRMMTGG FAISGQMVTL TDITCPILAF VGEVDDIGQP ASVRGIRRAA PNSEVYECLI RAGHFGLVVG S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc acccgatgat gatgtcggcg gacatgtggg acgtcacccg tgaagacggc gcggtgggga tcctgcacgc cagcgggcta gatccctggg tcatcgactt cggctcaccc gacgaggtcg agggcggaat gcgccgtaac ctggccgacc acatcgtcgc cctcagcgag gcggtcgata ccgtcaagga cgccactggc cacgatgtgc acttcgtcgg gtattcgcag ggtggcatgt tctgctatca ggccgcggca taccggcgtt cgaaggacat cgccagcgtg gtcgcgttcg gctcgccggt ggacaccctg gccgcgttgc ccatgggcat cccggcgaac atgggcgctg cggtcgccga tttcatggcc gatcacgtct tcaatcgctt ggatatccca agctggatgg cgcgcatggg ttttcagatg atggacccac tcaaaaccgc gaaggcccgg gtggacttcg tgcgtcagtt gcacgaccgc gaggcactgc tgccgcggga acaacagcgc cggttcctgg aatccgaagg atggatcgcc tggtcgggcc cggcgatctc ggaactgctc aagcagttca tcgcgcacaa ccgaatgatg acgggtggtt tcgccatcag cggccagatg gtgacgctta ccgatatcac ttgcccgata ctggcgttcg tcggtgaggt cgacgacatc ggccagccgg cgtcggtacg cggcatccgg cgggccgcgc ccaactccga ggtctacgaa tgtctcatcc gggcagggca tttcggtctc gtcgtgggat cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for MytuD.20304.a.B4.GE43504
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 93
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMN DHTRISLGRI IDEQAHDAPK GEFLLFDGRV HTYEAVNRRI NNVVRGLIAV GVRQGDRVGV LMETRPSALV AIAALSRLGA VAVVMRPDTD LSASVRLGRV TEILTDPTNL DAARQLPGQV LVLGGGESRD LDLPADALEQ GQVIDMEKID PDAVELPAWY RPNPGLARDL AFIAFSSADG DLVAKQITNY RWAXSAFGTA STAXLGRRDT VYCLTPLHHE SALLVSLGGA VVGGTRIALS RGLRPDXVRG RGTPVRRHRR LLHMGHAA
Validated NT Sequence
cgttggccgg taagtaccgc tgagcgttag ctgcggcacc acgtgcacga tgtcaggtcc cagccccacc ggcatgctcg ccacggcttc ggtcaagtcg gcggctgtga tggtcgctcc aggcagcaac gtcaccgccg acaccgcgac gtggcgaccg cgcaccaata caccgtaggt caccgcgagg tcgacaccgg tgatgaggcc caacgcgttg gtgaccggct cggtgtaaac catcccgcgc gcagtgcgca ccaccgagcc gcgtccaccc gccagccagt agtccccatc gtcgtcacgc cagaataggt agtcggtaga tatccaggtg tcggcgggag cgaagacacc gcgtttgacc gacgcggtcg gatcgatcgg ccctctggat tgtgcgagca gcaccccgac ctggttgaca ccggcgacct gcacgaagcc gcggtcgttc tccaggatca ggtcatgttc ggcgtcgtag gccccaagtt cgacacgtcc ggcgccaggc aacggacggc ccttgctgcc gatcttggcg ccagccacgt tggccagcac cgcctgtccg tcggtggtgg cgaaaaactc gacgacgtgc gccggtgcga acgcttcgac gacccgctcc cacaatccgg tcggcatgcc cgagccgatg aacaaccgca ccggatggtt gccgtgcaac acgaacgccg gatcgtcgac cacgtcacgc agcatggccc atgtgtagga gacgacggtg acgccgtact ggcgtacctc ggccacgaac cnggtccggg cgcaagccgc gggacaatgc gatacgggtt ccgcccacga ccgcgccgcc caggctgacc aacagtgcgg actcatggtg cagcggcgtc aaacagtaca ccgtgtctct gcggccgagg nccgccgtcg aggcggtccc gaaggccgac nccgcccagc ggtagttggt gatctgcttg gccaccaggt cgccgtcggc cgaactgaac gcgatgaacg ccagatcccg cgccaatccg ggattcggtc gataccacgc cggcaactcg acggcgtccg ggtcgatttt ttccatgtcg atgacttggc cctgttcaag tgcgtcggcc ggcagatcca gatcacgcga ttcaccacca cccaacacca gcacctgtcc gggcaactgg cgcgcagcat ccagattggt agggtcggtc aggatctcgg tcactctccc gagccggacc gacgcggaca ggtcggtgtc tggccgcatc accacggcaa ccgctcccag ccgagacagc gcggcgatgg cgaccagcgc gctgggccga gtctccatca gcacgccgac acggtcaccc tgccgcaccc cgaccgcgat gaggccacga acgacattgt tgatccgccg gtttaccgcc tcataggtgt gcacgcgccc gtcgaacaac aggaattcac ccttcggggc atcgtgtgcc tgttcgtcga tgatgcggcc cagcgagatc cgggtgtggt cgttcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHND HTRISLGRII DEQAHDAPKG EFLLFDGRVH TYEAVNRRIN NVVRGLIAVG VRQGDRVGVL METRPSALVA IAALSRLGAV AVVMRPDTDL SASVRLGRVT EILTDPTNLD AARQLPGQVL VLGGGESRDL DLPADALEQG QVIDMEKIDP DAVELPAWYR PNPGLARDLA FIAFSSADGD LVAKQITNYR WAVSAFGTAS TAALGRRDTV YCLTPLHHES ALLVSLGGAV VGGTRIALSR GLRPDRFVAE VRQYGVTVVS YTWAMLRDVV DDPAFVLHGN HPVRLFIGSG MPTGLWERVV EAFAPAHVVE FFATTDGQAV LANVAGAKIG SKGRPLPGAG RVELGAYDAE HDLILENDRG FVQVAGVNQV GVLLAQSRGP IDPTASVKRG VFAPADTWIS TDYLFWRDDD GDYWLAGGRG SVVRTARGMV YTEPVTNALG LITGVDLAVT YGVLVRGRHV AVSAVTLLPG ATITAADLTE AVASMPVGLG PDIVHVVPQL TLSGTYRPTV SALRAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacgacc acacccggat ctcgctgggc cgcatcatcg acgaacaggc acacgatgcc ccgaagggtg aattcctgtt gttcgacggg cgcgtgcaca cctatgaggc ggtaaaccgg cggatcaaca atgtcgttcg tggcctcatc gcggtcgggg tgcggcaggg tgaccgtgtc ggcgtgctga tggagactcg gcccagcgcg ctggtcgcca tcgccgcgct gtctcggctg ggagcggttg ccgtggtgat gcggccagac accgacctgt ccgcgtcggt ccggctcggg agagtgaccg agatcctgac cgaccctacc aatctggatg ctgcgcgcca gttgcccgga caggtgctgg tgttgggtgg tggtgaatcg cgtgatctgg atctgccggc cgacgcactt gaacagggcc aagtcatcga catggaaaaa atcgacccgg acgccgtcga gttgccggcg tggtatcgac cgaatcccgg attggcgcgg gatctggcgt tcatcgcgtt cagttcggcc gacggcgacc tggtggccaa gcagatcacc aactaccgct gggcggtgtc ggccttcggg accgcctcga cggcggccct cggccgcaga gacacggtgt actgtttgac gccgctgcac catgagtccg cactgttggt cagcctgggc ggcgcggtcg tgggcggaac ccgtatcgca ttgtcccgcg gcttgcgccc ggaccggttc gtggccgagg tacgccagta cggcgtcacc gtcgtctcct acacatgggc catgctgcgt gacgtggtcg acgatccggc gttcgtgttg cacggcaacc atccggtgcg gttgttcatc ggctcgggca tgccgaccgg attgtgggag cgggtcgtcg aagcgttcgc accggcgcac gtcgtcgagt ttttcgccac caccgacgga caggcggtgc tggccaacgt ggctggcgcc aagatcggca gcaagggccg tccgttgcct ggcgccggac gtgtcgaact tggggcctac gacgccgaac atgacctgat cctggagaac gaccgcggct tcgtgcaggt cgccggtgtc aaccaggtcg gggtgctgct cgcacaatcc agagggccga tcgatccgac cgcgtcggtc aaacgcggtg tcttcgctcc cgccgacacc tggatatcta ccgactacct attctggcgt gacgacgatg gggactactg gctggcgggt ggacgcggct cggtggtgcg cactgcgcgc gggatggttt acaccgagcc ggtcaccaac gcgttgggcc tcatcaccgg tgtcgacctc gcggtgacct acggtgtatt ggtgcgcggt cgccacgtcg cggtgtcggc ggtgacgttg ctgcctggag cgaccatcac agccgccgac ttgaccgaag ccgtggcgag catgccggtg gggctgggac ctgacatcgt gcacgtggtg ccgcagctaa cgctcagcgg tacttaccgg ccaacggtca gcgcgttgcg ggccaactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MytuD.20304.a.B5.GE43505
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS QIVESVPMYK LRRYFPPDNR PGQPPVGPPV LMVHPMMMSA DMWDVTREDG AVGILHASGL DPWVIDFGSP DEVEGGMRRN LADHIVALSE AVDTVKDATG HDVHFVGYSQ GGMFCYQAAA YRRSKDIASV VAFGSPVDTL AALPMGIPAN MGAAVADFMA DHVFNRLDIP SWMARMGFQM MDPLKTAKAR VDFVRQLHDR EALLPREQQR RFLESEGWIA WSGPAISELL KQFIAHNRMM TGGFAISGQM VTLTDITCPI LAFVGEVDDI GQPASVRGIR RAAPNSEVYE CLIRAGHFGL VVGSRAAQQS WPTVADWVR
Validated NT Sequence
tactcactta gcgcacccag tcggccacgg tcggccagct ctgttgtgcc gctcgggatc ccacgacgag accgaaatgc cctgcccgga tgagacattc gtagacctcg gagttgggcg cggcccgccg gatgccgcgt accgacgccg gctggccgat gtcgtcgacc tcaccgacga acgccagtat cgggcaagtg atatcggtaa gcgtcaccat ctggccgctg atggcgaaac cacccgtcat cattcggttg tgcgcgatga actgcttgag cagttccgag atcgccgggc ccgaccaggc gatccatcct tcggattcca ggaaccggcg ctgttgttcc cgcggcagca gtgcctcgcg gtcgtgcaac tgacgcacga agtccacccg ggccttcgcg gttttgagtg ggtccatcat ctgaaaaccc atgcgcgcca tccagcttgg gatatccaag cgattgaaga cgtgatcggc catgaaatcg gcgaccgcag cgcccatgtt cgccgggatg cccatgggca acgcggccag ggtgtccacc ggcgagccga acgcgaccac gctggcgatg tccttcgaac gccggtatgc cgcggcctga tagcagaaca tgccaccctg cgaatacccg acgaagtgca catcgtggcc agtggcgtcc ttgacggtat cgaccgcctc gctgagggcg acgatgtggt cggccaggtt acggcgcatt ccgccctcga cctcgtcggg tgagccgaag tcgatgaccc agggatctag cccgctggcg tgcaggatcc ccaccgcgcc gtcttcacgg gtgacgtccc acatgtccgc cgacatcatc atcgggtgca ccatcagcac cggcggaccc accggtggct gtcccgggcg gttgtccggc ggaaaatacc gccgcagctt gtacatcggt acgctctcaa cgatttggga catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHSQ IVESVPMYKL RRYFPPDNRP GQPPVGPPVL MVHPMMMSAD MWDVTREDGA VGILHASGLD PWVIDFGSPD EVEGGMRRNL ADHIVALSEA VDTVKDATGH DVHFVGYSQG GMFCYQAAAY RRSKDIASVV AFGSPVDTLA ALPMGIPANM GAAVADFMAD HVFNRLDIPS WMARMGFQMM DPLKTAKARV DFVRQLHDRE ALLPREQQRR FLESEGWIAW SGPAISELLK QFIAHNRMMT GGFAISGQMV TLTDITCPIL AFVGEVDDIG QPASVRGIRR AAPNSEVYEC LIRAGHFGLV VGSRAAQQSW PTVADWVR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcccaaa tcgttgagag cgtaccgatg tacaagctgc ggcggtattt tccgccggac aaccgcccgg gacagccacc ggtgggtccg ccggtgctga tggtgcaccc gatgatgatg tcggcggaca tgtgggacgt cacccgtgaa gacggcgcgg tggggatcct gcacgccagc gggctagatc cctgggtcat cgacttcggc tcacccgacg aggtcgaggg cggaatgcgc cgtaacctgg ccgaccacat cgtcgccctc agcgaggcgg tcgataccgt caaggacgcc actggccacg atgtgcactt cgtcgggtat tcgcagggtg gcatgttctg ctatcaggcc gcggcatacc ggcgttcgaa ggacatcgcc agcgtggtcg cgttcggctc gccggtggac accctggccg cgttgcccat gggcatcccg gcgaacatgg gcgctgcggt cgccgatttc atggccgatc acgtcttcaa tcgcttggat atcccaagct ggatggcgcg catgggtttt cagatgatgg acccactcaa aaccgcgaag gcccgggtgg acttcgtgcg tcagttgcac gaccgcgagg cactgctgcc gcgggaacaa cagcgccggt tcctggaatc cgaaggatgg atcgcctggt cgggcccggc gatctcggaa ctgctcaagc agttcatcgc gcacaaccga atgatgacgg gtggtttcgc catcagcggc cagatggtga cgcttaccga tatcacttgc ccgatactgg cgttcgtcgg tgaggtcgac gacatcggcc agccggcgtc ggtacgcggc atccggcggg ccgcgcccaa ctccgaggtc tacgaatgtc tcatccgggc agggcatttc ggtctcgtcg tgggatcccg agcggcacaa cagagctggc cgaccgtggc cgactgggtg cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.20304.a.HZ1.GE43745
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 121kDa
OBSERVED MW: 121kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 26
Validated AA Sequence
MLRDVVDDPA FVLHXNXPVR LFIGSGMPXG LWERVVEAFA PAHVVEFFAT TDGQAVLANV AGAKIGSKGR PLPGAGRVEL GAYDAEHDLI LENDRGFVQV AGVNQVGVLL AQSRGPIDPT ASVKRGVFAP ADTWISTDYL FWRDDDGDYW LAGGRGSVVR TARGMVYTEP VTNALGLITG VDLAVTYGVL VRGRHVAVSA VTLLPGATIT AADLTEAVAS MPVGLGPDIV HVVPQLTLSG TYRPTVSALR ANGIPKAGRQ AWYFNSGGNE YRRLTPAVRT ELTGQHRRGN A
Validated NT Sequence
ggctttgttt agcagccgga tcctcgagaa ngcttggctg cagaacttgt tcgtgctgtt tattaagcat tgccgcgccg atgctggccg gtcaactcgg tgcggaccgc cggcgtcaac cgccggtact cgttgccgcc ggagttgaaa taccatgcct gacggcccgc cttgggaatc ccgttggccc gcaacgcgct gaccgttggc cggtaagtac cgctgagcgt tagctgcggc accacgtgca cgatgtcagg tcccagcccc accggcatgc tcgccacggc ttcggtcaag tcggcggctg tgatggtcgc tccaggcagc aacgtcaccg ccgacaccgc gacgtggcga ccgcgcacca atacaccgta ggtcaccgcg aggtcgacac cggtgatgag gcccaacgcg ttggtgaccg gctcggtgta aaccatcccg cgcgcagtgc gcaccaccga gccgcgtcca cccgccagcc agtagtcccc atcgtcgtca cgccagaata ggtagtcggt agatatccag gtgtcggcgg gagcgaagac accgcgtttg accgacgcgg tcggatcgat cggccctctg gattgtgcga gcagcacccc gacctggttg acaccggcga cctgcacgaa gccgcggtcg ttctccagga tcaggtcatg ttcggcgtcg taggccccaa gttcgacacg tccggcgcca ggcaacggac ggcccttgct gccgatcttg gcgccagcca cgttggccag caccgcctgt ccgtcggtgg tggcgaaaaa ctcgacgacg tgcgccggtg cgaacgcttc gacgacccgc tcccacaatc cggncggcat gcccgagccg atgaacaacc gcaccggatn gttgncgtgc aacacgaacg ccggatcgtc gaccacgtca cgcagcatgn gccnnnnnnt gtnggannac naangtgann ccgtactggc gtacctnggn cacgaaccgg tccgggcnca agccgcggga gncgatacng gtttccnncc cacnaac
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMV DLNFSMVTRP IERLVATAQN GLEVLRLGGL ETGSVPSPSQ IVESVPMYKL RRYFPPDNRP GQPPVGPPVL MVHPMMMSAD MWDVTREDGA VGILHASGLD PWVIDFGSPD EVEGGMRRNL ADHIVALSEA VDTVKDATGH DVHFVGYSQG GMFCYQAAAY RRSKDIASVV AFGSPVDTLA ALPMGIPANM GAAVADFMAD HVFNRLDIPS WMARMGFQMM DPLKTAKARV DFVRQLHDRE ALLPREQQRR FLESEGWIAW SGPAISELLK QFIAHNRMMT GGFAISGQMV TLTDITCPIL AFVGEVDDIG QPASVRGIRR AAPNSEVYEC LIRAGHFGLV VGSRAAQQSW PTVADWVRWI SGDGTKPENI HLMADQPAEH TDSGVAFSSR VAHGIGEVSE AALALARGAA DAVVAANRSV RTLAVETVRT LPRLARLGQL NDHTRISLGR IIDEQAHDAP KGEFLLFDGR VHTYEAVNRR INNVVRGLIA VGVRQGDRVG VLMETRPSAL VAIAALSRLG AVAVVMRPDT DLSASVRLGR VTEILTDPTN LDAARQLPGQ VLVLGGGESR DLDLPADALE QGQVIDMEKI DPDAVELPAW YRPNPGLARD LAFIAFSSAD GDLVAKQITN YRWAVSAFGT ASTAALGRRD TVYCLTPLHH ESALLVSLGG AVVGGTRIAL SRGLRPDRFV AEVRQYGVTV VSYTWAMLRD VVDDPAFVLH GNHPVRLFIG SGMPTGLWER VVEAFAPAHV VEFFATTDGQ AVLANVAGAK IGSKGRPLPG AGRVELGAYD AEHDLILEND RGFVQVAGVN QVGVLLAQSR GPIDPTASVK RGVFAPADTW ISTDYLFWRD DDGDYWLAGG RGSVVRTARG MVYTEPVTNA LGLITGVDLA VTYGVLVRGR HVAVSAVTLL PGATITAADL TEAVASMPVG LGPDIVHVVP QLTLSGTYRP TVSALRANGI PKAGRQAWYF NSGGNEYRRL TPAVRTELTG QHRRGNA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg tggtggatct caatttttcg atggtcacgc gaccaatcga gcgcctggtg gccacggcgc agaacggtct ggaagtcctg cgactcgggg gcctggaaac cggcagtgtt ccgtcgccgt cccaaatcgt tgagagcgta ccgatgtaca agctgcggcg gtattttccg ccggacaacc gcccgggaca gccaccggtg ggtccgccgg tgctgatggt gcacccgatg atgatgtcgg cggacatgtg ggacgtcacc cgtgaagacg gcgcggtggg gatcctgcac gccagcgggc tagatccctg ggtcatcgac ttcggctcac ccgacgaggt cgagggcgga atgcgccgta acctggccga ccacatcgtc gccctcagcg aggcggtcga taccgtcaag gacgccactg gccacgatgt gcacttcgtc gggtattcgc agggtggcat gttctgctat caggccgcgg cataccggcg ttcgaaggac atcgccagcg tggtcgcgtt cggctcgccg gtggacaccc tggccgcgtt gcccatgggc atcccggcga acatgggcgc tgcggtcgcc gatttcatgg ccgatcacgt cttcaatcgc ttggatatcc caagctggat ggcgcgcatg ggttttcaga tgatggaccc actcaaaacc gcgaaggccc gggtggactt cgtgcgtcag ttgcacgacc gcgaggcact gctgccgcgg gaacaacagc gccggttcct ggaatccgaa ggatggatcg cctggtcggg cccggcgatc tcggaactgc tcaagcagtt catcgcgcac aaccgaatga tgacgggtgg tttcgccatc agcggccaga tggtgacgct taccgatatc acttgcccga tactggcgtt cgtcggtgag gtcgacgaca tcggccagcc ggcgtcggta cgcggcatcc ggcgggccgc gcccaactcc gaggtctacg aatgtctcat ccgggcaggg catttcggtc tcgtcgtggg atcccgagcg gcacaacaga gctggccgac cgtggccgac tgggtgcgct ggatctccgg cgacggcacc aaaccggaaa acatccacct gatggccgat cagccggccg aacacaccga tagcggtgtg gctttcagct cccgggtcgc gcacggcatc ggggaggtct cggaggctgc gttggcgctg gctcgcggcg cggccgacgc ggtcgttgcg gccaacagat cggtgcgcac gctggcggtg gagacggtgc ggacgctgcc gcgactagcc cggttgggtc agctcaacga ccacacccgg atctcgctgg gccgcatcat cgacgaacag gcacacgatg ccccgaaggg tgaattcctg ttgttcgacg ggcgcgtgca cacctatgag gcggtaaacc ggcggatcaa caatgtcgtt cgtggcctca tcgcggtcgg ggtgcggcag ggtgaccgtg tcggcgtgct gatggagact cggcccagcg cgctggtcgc catcgccgcg ctgtctcggc tgggagcggt tgccgtggtg atgcggccag acaccgacct gtccgcgtcg gtccggctcg ggagagtgac cgagatcctg accgacccta ccaatctgga tgctgcgcgc cagttgcccg gacaggtgct ggtgttgggt ggtggtgaat cgcgtgatct ggatctgccg gccgacgcac ttgaacaggg ccaagtcatc gacatggaaa aaatcgaccc ggacgccgtc gagttgccgg cgtggtatcg accgaatccc ggattggcgc gggatctggc gttcatcgcg ttcagttcgg ccgacggcga cctggtggcc aagcagatca ccaactaccg ctgggcggtg tcggccttcg ggaccgcctc gacggcggcc ctcggccgca gagacacggt gtactgtttg acgccgctgc accatgagtc cgcactgttg gtcagcctgg gcggcgcggt cgtgggcgga acccgtatcg cattgtcccg cggcttgcgc ccggaccggt tcgtggccga ggtacgccag tacggcgtca ccgtcgtctc ctacacatgg gccatgctgc gtgacgtggt cgacgatccg gcgttcgtgt tgcacggcaa ccatccggtg cggttgttca tcggctcggg catgccgacc ggattgtggg agcgggtcgt cgaagcgttc gcaccggcgc acgtcgtcga gtttttcgcc accaccgacg gacaggcggt gctggccaac gtggctggcg ccaagatcgg cagcaagggc cgtccgttgc ctggcgccgg acgtgtcgaa cttggggcct acgacgccga acatgacctg atcctggaga acgaccgcgg cttcgtgcag gtcgccggtg tcaaccaggt cggggtgctg ctcgcacaat ccagagggcc gatcgatccg accgcgtcgg tcaaacgcgg tgtcttcgct cccgccgaca cctggatatc taccgactac ctattctggc gtgacgacga tggggactac tggctggcgg gtggacgcgg ctcggtggtg cgcactgcgc gcgggatggt ttacaccgag ccggtcacca acgcgttggg cctcatcacc ggtgtcgacc tcgcggtgac ctacggtgta ttggtgcgcg gtcgccacgt cgcggtgtcg gcggtgacgt tgctgcctgg agcgaccatc acagccgccg acttgaccga agccgtggcg agcatgccgg tggggctggg acctgacatc gtgcacgtgg tgccgcagct aacgctcagc ggtacttacc ggccaacggt cagcgcgttg cgggccaacg ggattcccaa ggcgggccgt caggcatggt atttcaactc cggcggcaac gagtaccggc ggttgacgcc ggcggtccgc accgagttga ccggccagca tcggcgcggc aatgcttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.20304.a.HZ2.GE43746
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREXXXGTLE AQTQGPGSMX XDLNFSMVTR PIERLVATAX NGLEVLRLGG LEXXSXPSPS XIVXSVPMYK LRRYFPPDNR PGXPPXGPPV LMVHPMMXSA DMWDVTREDG AVGILHASGL DPWVIDFGSP DEVXXGMRRN XXDHIVAXSE AVXTVKDATG HDVHXVGXSQ XGMXCYQAAA YRRSKDIASV VAFGSPXXXX AALPMGIPAN MGAAVADFMA DHVFNRLDIP SWMARMGFQM MDPLKTAKAR VDFVRQLHDR EALLPREQQR RFLESEGWIA WSGPAISELL KQFIAHNRMM TGGFAISGQM VTLTDITCPI LAFVGEVDDI GQPASVRGIR RAAPNSEVYE CLIRAGHFGL VVGSRAAQQS WPTVADWVRW ISGDGT
Validated NT Sequence
tggtgccgtc gccggagatc cagcgcaccc agtcggccac ggtcggccag ctctgttgtg ccgctcggga tcccacgacg agaccgaaat gccctgcccg gatgagacat tcgtagacct cggagttggg cgcggcccgc cggatgccgc gtaccgacgc cggctggccg atgtcgtcga cctcaccgac gaacgccagt atcgggcaag tgatatcggt aagcgtcacc atctggccgc tgatggcgaa accacccgtc atcattcggt tgtgcgcgat gaactgcttg agcagttccg agatcgccgg gcccgaccag gcgatccatc cttcggattc caggaaccgg cgctgttgtt cccgcggcag cagtgcctcg cggtcgtgca actgacgcac gaagtccacc cgggccttcg cggttttgag tgggtccatc atctgaaaac ccatgcgcgc catccagctt gggatatcca agcgattgaa gacgtgatcg gccatgaaat cggcgaccgc agcgcccatg ttcgccggga tgcccatggg caacgcggcc anggngnncn ccggcgagcc gaacgcgacc acgctggcga tgtccttcga acgccggtat gccgcggcct gatagcagan catgccncnc tgcgannacc cgacgangtg cacatcgtgg ccagtggcgt ccttgacggt ntcgaccgcc tcgctgangg cgacgatgtg gtcggncang ttacggcgca ttccnncnnn gacctcgtcg ggtgagccga agtcgatgac ccagggatct agcccgctgg cgtgcaggat ccccaccgcn ccgtcttcac gggtgacgtc ccacatgtcc gcngacntca tcatcgggtg caccatcagc accggcggac ccnccggtgg ctnnccnggg cggttgtccg gcggaaaata ccgccgcagc ttgtacatcg gtacgctntc aacgattnng gacggcgacg gancactnnc gnnttccagg cccccgagtc gcaggacttc cagaccgttn tgcgccgtgg ccaccaggcg ctcgattggt cgcgtgacca tcgaaaaatt gagatccncc nccatcgaac caggaccctg ggtctgagct tccagggtac cnncnntnnn ttctctgtga gcctcaataa tatcgttatc ctccatgtcc aaatcttcag gggtctgatc agcttgaatt ctaataccgt cgtacaagaa tcttaaggag tccatttcct taccctgtct tttagcgaac gcttccatca gccttcttaa aggagtggtc tttttgatct tgaagaagat ctctgaagat ccatcggaca cctttaaatt gatgtgagtc tcaggcttga cttctggctt gacctctggc ttagcttctt gattgacttc tgagtccgac ttcatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMV DLNFSMVTRP IERLVATAQN GLEVLRLGGL ETGSVPSPSQ IVESVPMYKL RRYFPPDNRP GQPPVGPPVL MVHPMMMSAD MWDVTREDGA VGILHASGLD PWVIDFGSPD EVEGGMRRNL ADHIVALSEA VDTVKDATGH DVHFVGYSQG GMFCYQAAAY RRSKDIASVV AFGSPVDTLA ALPMGIPANM GAAVADFMAD HVFNRLDIPS WMARMGFQMM DPLKTAKARV DFVRQLHDRE ALLPREQQRR FLESEGWIAW SGPAISELLK QFIAHNRMMT GGFAISGQMV TLTDITCPIL AFVGEVDDIG QPASVRGIRR AAPNSEVYEC LIRAGHFGLV VGSRAAQQSW PTVADWVRWI SGDGTKP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg tggtggatct caatttttcg atggtcacgc gaccaatcga gcgcctggtg gccacggcgc agaacggtct ggaagtcctg cgactcgggg gcctggaaac cggcagtgtt ccgtcgccgt cccaaatcgt tgagagcgta ccgatgtaca agctgcggcg gtattttccg ccggacaacc gcccgggaca gccaccggtg ggtccgccgg tgctgatggt gcacccgatg atgatgtcgg cggacatgtg ggacgtcacc cgtgaagacg gcgcggtggg gatcctgcac gccagcgggc tagatccctg ggtcatcgac ttcggctcac ccgacgaggt cgagggcgga atgcgccgta acctggccga ccacatcgtc gccctcagcg aggcggtcga taccgtcaag gacgccactg gccacgatgt gcacttcgtc gggtattcgc agggtggcat gttctgctat caggccgcgg cataccggcg ttcgaaggac atcgccagcg tggtcgcgtt cggctcgccg gtggacaccc tggccgcgtt gcccatgggc atcccggcga acatgggcgc tgcggtcgcc gatttcatgg ccgatcacgt cttcaatcgc ttggatatcc caagctggat ggcgcgcatg ggttttcaga tgatggaccc actcaaaacc gcgaaggccc gggtggactt cgtgcgtcag ttgcacgacc gcgaggcact gctgccgcgg gaacaacagc gccggttcct ggaatccgaa ggatggatcg cctggtcggg cccggcgatc tcggaactgc tcaagcagtt catcgcgcac aaccgaatga tgacgggtgg tttcgccatc agcggccaga tggtgacgct taccgatatc acttgcccga tactggcgtt cgtcggtgag gtcgacgaca tcggccagcc ggcgtcggta cgcggcatcc ggcgggccgc gcccaactcc gaggtctacg aatgtctcat ccgggcaggg catttcggtc tcgtcgtggg atcccgagcg gcacaacaga gctggccgac cgtggccgac tgggtgcgct ggatctccgg cgacggcacc aaaccgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.20304.a.HZ5.GE43748
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 24
PERCENT COVERAGE: 42
Validated AA Sequence
MADXXVXXSX AVDTVKGRXW TRCALRRVLA GWXXXXSXXG IPAFEGHRQR GRVRLAGGHP GRVAHGHPGE HGRCGRRFHX RSRLQSLGYP KLDGAHGXSD DGPTQXREGP GGLRASVARP XGTXAXXTTA PVHGIRKXDR XXGPGDLGTA XAVHRAQPND DGWFRHQRPD GDAYRYHLPD TGVRR
Validated NT Sequence
gccccgctnc ngnnnannnc acgacnanac cgaaatngcc ctngcccgga tganacattc ganacctcgg agttgggcgc ggcccgccgg atgccgcgta ccgacgccgg ctggccgatg tcntccacct caccgacgaa cgccagtatc gggcaagtga tatcggtaag cgtcaccatc tggccgctga tggcgaaacc acccgtcatc attcggttgt gcgcgatgaa ctgctngagc agttccgaga tcgccgggcc cgncctnacg atccnncttn cggattccat gaaccggcgc tgttgttncc ncggcagnag tgcctnncgg tcgtgcaact gacgcacgaa gtccacccgg gccttcgcgg ntttgagtgg gtccatcatc tganaancca tgcgcgccat ccagcttggg atatccaagc gattgaagac gtgatctgnc atgaaatcgt cgaccgcagc gcccatgttc tccgggatgc ccatgggcaa cgcggccagg gtgtccaccg gcgagccgaa cgcgaccacg ctggcgatgt ccttcgaacg ccggtatgcc gnngnctgat annanancnt gccaccctgc taatacccga cgaagtgcac atcgtgtcca gnggcgtccc ttgacggtat cnaccgcntc gctgangnng acnatgtngt cggccatgtn acggcgcant tncnnnntct acctcntcgg gtgagcnnga agtcgatgac cannggatct agcccgctgc cgtgcangan ncccaacnng ccgcttcncg gntgncgtcc canatgnccg ncgnntcatc atcggnnnna tcntcngcac ngcggaaccc ancggnnntg tcccngnnnn nnnccngnnn naaantaccn ncntnagctg nannncggta cgctctnnnc ggatnnnggt annna
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSSQ IVESVPMYKL RRYFPPDNRP GQPPVGPPVL MVHPMMMSAD MWDVTREDGA VGILHASGLD PWVIDFGSPD EVEGGMRRNL ADHIVALSEA VDTVKDATGH DVHFVGYSQG GMFCYQAAAY RRSKDIASVV AFGSPVDTLA ALPMGIPANM GAAVADFMAD HVFNRLDIPS WMARMGFQMM DPLKTAKARV DFVRQLHDRE ALLPREQQRR FLESEGWIAW SGPAISELLK QFIAHNRMMT GGFAISGQMV TLTDITCPIL AFVGEVDDIG QPASVRGIRR AAPNSEVYEC LIRAGHFGLV VGSRAAQQSW PTVADWVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgt cccaaatcgt tgagagcgta ccgatgtaca agctgcggcg gtattttccg ccggacaacc gcccgggaca gccaccggtg ggtccgccgg tgctgatggt gcacccgatg atgatgtcgg cggacatgtg ggacgtcacc cgtgaagacg gcgcggtggg gatcctgcac gccagcgggc tagatccctg ggtcatcgac ttcggctcac ccgacgaggt cgagggcgga atgcgccgta acctggccga ccacatcgtc gccctcagcg aggcggtcga taccgtcaag gacgccactg gccacgatgt gcacttcgtc gggtattcgc agggtggcat gttctgctat caggccgcgg cataccggcg ttcgaaggac atcgccagcg tggtcgcgtt cggctcgccg gtggacaccc tggccgcgtt gcccatgggc atcccggcga acatgggcgc tgcggtcgcc gatttcatgg ccgatcacgt cttcaatcgc ttggatatcc caagctggat ggcgcgcatg ggttttcaga tgatggaccc actcaaaacc gcgaaggccc gggtggactt cgtgcgtcag ttgcacgacc gcgaggcact gctgccgcgg gaacaacagc gccggttcct ggaatccgaa ggatggatcg cctggtcggg cccggcgatc tcggaactgc tcaagcagtt catcgcgcac aaccgaatga tgacgggtgg tttcgccatc agcggccaga tggtgacgct taccgatatc acttgcccga tactggcgtt cgtcggtgag gtcgacgaca tcggccagcc ggcgtcggta cgcggcatcc ggcgggccgc gcccaactcc gaggtctacg aatgtctcat ccgggcaggg catttcggtc tcgtcgtggg atcccgagcg gcacaacaga gctggccgac cgtggccgac tgggtgcgct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa