MytuD.19483.a

Dephospho-CoA kinase CoaE (Rv1631)

CENTER ID: MytuD.19483.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.19483.a.B1.GE41032 full length 1 407
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.19483.a.B1.PW38198 full length 1 407

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1815
TubercuList: Rv1631
MTB Network Portal: Rv1631
UniProt: P9WPA3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRIGLTGGI GAGKSLLSTT FSQCGGIVVD GDVLAREVVQ PGTEGLASLV DAFGRDILLA DGALDRQALA AKAFRDDESR GVLNGIVHPL VARRRSEIIA AVSGDAVVVE DIPLLVESGM APLFPLVVVV HADVELRVRR LVEQRGMAEA DARARIAAQA SDQQRRAVAD VWLDNSGSPE DLVRRARDVW NTRVQPFAHN LAQRQIARAP ARLVPADPSW PDQARRIVNR LKIACGHKAL RVDHIGSTAV SGFPDFLAKD VIDIQVTVES LDVADELAEP LLAAGYPRLE HITQDTEKTD ARSTVGRYDH TDSAALWHKR VHASADPGRP TNVHLRVHGW PNQQFALLFV DWLAANPGAR EDYLTVKCDA DRRADGELAR YVTAKEPWFL DAYQRAWEWA DAVHWRP
NT Sequence
atgctgcgca tcgggctgac cggcggcatt ggcgccggga agtcgttgct gtccacgacg ttctcgcaat gcggcggaat cgttgtcgac ggcgatgtgt tggcgcgtga agtggtccag ccgggcaccg aggggctggc ctcgctggtc gacgcgttcg gtcgcgacat cctgcttgca gacggagcgc tggaccggca ggcgttggcg gccaaggcgt ttcgagatga cgagtcgcgc ggtgtgctca acggaatcgt gcacccgctg gtcgcccggc gccgatccga gatcatcgcg gcggtttcgg gggacgcggt tgtggtcgaa gatattccac tgctggtgga atccgggatg gcgccattgt ttccgctggt ggtggtggtg cacgccgacg tcgagctacg ggtgcgacgg ctggtcgagc aacgcggcat ggccgaagcc gacgcccggg ctaggatcgc tgcgcaggcc agcgaccagc agcgtcgtgc cgtcgccgac gtctggctgg acaactcggg cagcccagag gatttggtgc ggcgggcccg cgacgtctgg aacacgcgcg tccagccctt cgcgcacaac ctggcccaac gtcagattgc gcgcgcgccg gctaggttgg tgccggcgga tccaagctgg ccggatcagg cgcggcgcat cgtcaaccgg ctaaagatcg cgtgcgggca taaggccttg cgagttgacc acattgggtc aaccgccgtg tcgggcttcc ccgattttct agccaaggat gtcatcgaca tccaggtcac cgtcgaatca cttgacgtgg ccgacgagct ggccgagccc ttgctggccg ccggctaccc acgcctcgag cacatcaccc aggacaccga aaagaccgac gctcgcagca ccgtcggccg ctacgaccac accgacagtg ccgctctgtg gcacaagcgc gtgcacgcct cggcggatcc cggtcggccg accaacgtgc acctgcgggt gcacggctgg cccaaccaac agttcgccct gctgttcgtc gactggctgg cggccaatcc cggcgcgaga gaagactatt tgacggtcaa gtgtgacgcc gacaggcgcg ccgacggtga gctcgcgcgc tacgtcaccg ccaaggagcc gtggttcctg gatgcctacc agcgggcatg ggagtgggcg gatgcggtgc actggcgtcc ctga
Details for MytuD.19483.a.B1.GE41032
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML RIGLTGGIGA GKSLLSTTFS QCGGIVVDGD VLAREVVQPG TEGLASLVDA FGRDILLADG ALDRQALAAK AFRDDESRGV LNGIVHPLVA RRRSEIIAAV SGDAVVVEDI PLLVESGMAP LFPLVVVVHA DVELRVRRLV EQRGMAEADA RARIAAQASD QQRRAVADVW LDNSGSPEDL VRRARDVWNT RVQPFAHNLA QRQIARAPAR LVPADPSWPD QARRIVNRLK IACGHKALRV DHIGSTAVSG FPDFLAKDVI DIQVTVESLD VADELAEPLL AAGYPRLEHI TQDTEKTDAR STVGRYDHTD SAALWHKRVH ASADPGRPTN VHLRVHGWPN QQFALLFVDW LAANPGARED YLTVKCDADR RADGELARYV TAKEPWFLDA YQRAWEW
Validated NT Sequence
cccactccca tgcccgctgg taggcatcca ggaaccacgg ctccttggcg gtgacgtagc gcgcgagctc accgtcggcg cgcctgtcgg cgtcacactt gaccgtcaaa tagtcttctc tcgcgccggg attggccgcc agccagtcga cgaacagcag ggcgaactgt tggttgggcc agccgtgcac ccgcaggtgc acgttggtcg gccgaccggg atccgccgag gcgtgcacgc gcttgtgcca cagagcggca ctgtcggtgt ggtcgtagcg gccgacggtg ctgcgagcgt cggtcttttc ggtgtcctgg gtgatgtgct cgaggcgtgg gtagccggcg gccagcaagg gctcggccag ctcgtcggcc acgtcaagtg attcgacggt gacctggatg tcgatgacat ccttggctag aaaatcgggg aagcccgaca cggcggttga cccaatgtgg tcaactcgca aggccttatg cccgcacgcg atctttagcc ggttgacgat gcgccgcgcc tgatccggcc agcttggatc cgccggcacc aacctagccg gcgcgcgcgc aatctgacgt tgggccaggt tgtgcgcgaa gggctggacg cgcgtgttcc agacgtcgcg ggcccgccgc accaaatcct ctgggctgcc cgagttgtcc agccagacgt cggcgacggc acgacgctgc tggtcgctgg cctgcgcagc gatcctagcc cgggcgtcgg cttcggccat gccgcgttgc tcgaccagcc gtcgcacccg tagctcgacg tcggcgtgca ccaccaccac cagcggaaac aatggcgcca tcccggattc caccagcagt ggaatatctt cgaccacaac cgcgtccccc gaaaccgccg cgatgatctc ggatcggcgc cgggcgacca gcgggtgcac gattccgttg agcacaccgc gcgactcgtc atctcgaaac gccttggccg ccaacgcctg ccggtccagc gctccgtctg caagcaggat gtcgcgaccg aacgcgtcga ccagcgaggc cagcccctcg gtgcccggct ggaccacttc acgcgccaac acatcgccgt cgacaacgat tccgccgcat tgcgagaacg tcgtggacag caacgacttc ccggcgccaa tgccgccggt cagcccgatg cgcagcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHML RIGLTGGIGA GKSLLSTTFS QCGGIVVDGD VLAREVVQPG TEGLASLVDA FGRDILLADG ALDRQALAAK AFRDDESRGV LNGIVHPLVA RRRSEIIAAV SGDAVVVEDI PLLVESGMAP LFPLVVVVHA DVELRVRRLV EQRGMAEADA RARIAAQASD QQRRAVADVW LDNSGSPEDL VRRARDVWNT RVQPFAHNLA QRQIARAPAR LVPADPSWPD QARRIVNRLK IACGHKALRV DHIGSTAVSG FPDFLAKDVI DIQVTVESLD VADELAEPLL AAGYPRLEHI TQDTEKTDAR STVGRYDHTD SAALWHKRVH ASADPGRPTN VHLRVHGWPN QQFALLFVDW LAANPGARED YLTVKCDADR RADGELARYV TAKEPWFLDA YQRAWEWADA VHWRP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcatcgggct gaccggcggc attggcgccg ggaagtcgtt gctgtccacg acgttctcgc aatgcggcgg aatcgttgtc gacggcgatg tgttggcgcg tgaagtggtc cagccgggca ccgaggggct ggcctcgctg gtcgacgcgt tcggtcgcga catcctgctt gcagacggag cgctggaccg gcaggcgttg gcggccaagg cgtttcgaga tgacgagtcg cgcggtgtgc tcaacggaat cgtgcacccg ctggtcgccc ggcgccgatc cgagatcatc gcggcggttt cgggggacgc ggttgtggtc gaagatattc cactgctggt ggaatccggg atggcgccat tgtttccgct ggtggtggtg gtgcacgccg acgtcgagct acgggtgcga cggctggtcg agcaacgcgg catggccgaa gccgacgccc gggctaggat cgctgcgcag gccagcgacc agcagcgtcg tgccgtcgcc gacgtctggc tggacaactc gggcagccca gaggatttgg tgcggcgggc ccgcgacgtc tggaacacgc gcgtccagcc cttcgcgcac aacctggccc aacgtcagat tgcgcgcgcg ccggctaggt tggtgccggc ggatccaagc tggccggatc aggcgcggcg catcgtcaac cggctaaaga tcgcgtgcgg gcataaggcc ttgcgagttg accacattgg gtcaaccgcc gtgtcgggct tccccgattt tctagccaag gatgtcatcg acatccaggt caccgtcgaa tcacttgacg tggccgacga gctggccgag cccttgctgg ccgccggcta cccacgcctc gagcacatca cccaggacac cgaaaagacc gacgctcgca gcaccgtcgg ccgctacgac cacaccgaca gtgccgctct gtggcacaag cgcgtgcacg cctcggcgga tcccggtcgg ccgaccaacg tgcacctgcg ggtgcacggc tggcccaacc aacagttcgc cctgctgttc gtcgactggc tggcggccaa tcccggcgcg agagaagact atttgacggt caagtgtgac gccgacaggc gcgccgacgg tgagctcgcg cgctacgtca ccgccaagga gccgtggttc ctggatgcct accagcgggc atgggagtgg gcggatgcgg tgcactggcg tccctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MytuD.19483.a.B1.PW38198
PURIFICATION DATe: 2/27/2017
CONCENTRATION: 23mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML RIGLTGGIGA GKSLLSTTFS QCGGIVVDGD VLAREVVQPG TEGLASLVDA FGRDILLADG ALDRQALAAK AFRDDESRGV LNGIVHPLVA RRRSEIIAAV SGDAVVVEDI PLLVESGMAP LFPLVVVVHA DVELRVRRLV EQRGMAEADA RARIAAQASD QQRRAVADVW LDNSGSPEDL VRRARDVWNT RVQPFAHNLA QRQIARAPAR LVPADPSWPD QARRIVNRLK IACGHKALRV DHIGSTAVSG FPDFLAKDVI DIQVTVESLD VADELAEPLL AAGYPRLEHI TQDTEKTDAR STVGRYDHTD SAALWHKRVH ASADPGRPTN VHLRVHGWPN QQFALLFVDW LAANPGARED YLTVKCDADR RADGELARYV TAKEPWFLDA YQRAWEW
Validated NT Sequence
cccactccca tgcccgctgg taggcatcca ggaaccacgg ctccttggcg gtgacgtagc gcgcgagctc accgtcggcg cgcctgtcgg cgtcacactt gaccgtcaaa tagtcttctc tcgcgccggg attggccgcc agccagtcga cgaacagcag ggcgaactgt tggttgggcc agccgtgcac ccgcaggtgc acgttggtcg gccgaccggg atccgccgag gcgtgcacgc gcttgtgcca cagagcggca ctgtcggtgt ggtcgtagcg gccgacggtg ctgcgagcgt cggtcttttc ggtgtcctgg gtgatgtgct cgaggcgtgg gtagccggcg gccagcaagg gctcggccag ctcgtcggcc acgtcaagtg attcgacggt gacctggatg tcgatgacat ccttggctag aaaatcgggg aagcccgaca cggcggttga cccaatgtgg tcaactcgca aggccttatg cccgcacgcg atctttagcc ggttgacgat gcgccgcgcc tgatccggcc agcttggatc cgccggcacc aacctagccg gcgcgcgcgc aatctgacgt tgggccaggt tgtgcgcgaa gggctggacg cgcgtgttcc agacgtcgcg ggcccgccgc accaaatcct ctgggctgcc cgagttgtcc agccagacgt cggcgacggc acgacgctgc tggtcgctgg cctgcgcagc gatcctagcc cgggcgtcgg cttcggccat gccgcgttgc tcgaccagcc gtcgcacccg tagctcgacg tcggcgtgca ccaccaccac cagcggaaac aatggcgcca tcccggattc caccagcagt ggaatatctt cgaccacaac cgcgtccccc gaaaccgccg cgatgatctc ggatcggcgc cgggcgacca gcgggtgcac gattccgttg agcacaccgc gcgactcgtc atctcgaaac gccttggccg ccaacgcctg ccggtccagc gctccgtctg caagcaggat gtcgcgaccg aacgcgtcga ccagcgaggc cagcccctcg gtgcccggct ggaccacttc acgcgccaac acatcgccgt cgacaacgat tccgccgcat tgcgagaacg tcgtggacag caacgacttc ccggcgccaa tgccgccggt cagcccgatg cgcagcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHML RIGLTGGIGA GKSLLSTTFS QCGGIVVDGD VLAREVVQPG TEGLASLVDA FGRDILLADG ALDRQALAAK AFRDDESRGV LNGIVHPLVA RRRSEIIAAV SGDAVVVEDI PLLVESGMAP LFPLVVVVHA DVELRVRRLV EQRGMAEADA RARIAAQASD QQRRAVADVW LDNSGSPEDL VRRARDVWNT RVQPFAHNLA QRQIARAPAR LVPADPSWPD QARRIVNRLK IACGHKALRV DHIGSTAVSG FPDFLAKDVI DIQVTVESLD VADELAEPLL AAGYPRLEHI TQDTEKTDAR STVGRYDHTD SAALWHKRVH ASADPGRPTN VHLRVHGWPN QQFALLFVDW LAANPGARED YLTVKCDADR RADGELARYV TAKEPWFLDA YQRAWEWADA VHWRP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTGC GCATCGGGCT GACCGGCGGC ATTGGCGCCG GGAAGTCGTT GCTGTCCACG ACGTTCTCGC AATGCGGCGG AATCGTTGTC GACGGCGATG TGTTGGCGCG TGAAGTGGTC CAGCCGGGCA CCGAGGGGCT GGCCTCGCTG GTCGACGCGT TCGGTCGCGA CATCCTGCTT GCAGACGGAG CGCTGGACCG GCAGGCGTTG GCGGCCAAGG CGTTTCGAGA TGACGAGTCG CGCGGTGTGC TCAACGGAAT CGTGCACCCG CTGGTCGCCC GGCGCCGATC CGAGATCATC GCGGCGGTTT CGGGGGACGC GGTTGTGGTC GAAGATATTC CACTGCTGGT GGAATCCGGG ATGGCGCCAT TGTTTCCGCT GGTGGTGGTG GTGCACGCCG ACGTCGAGCT ACGGGTGCGA CGGCTGGTCG AGCAACGCGG CATGGCCGAA GCCGACGCCC GGGCTAGGAT CGCTGCGCAG GCCAGCGACC AGCAGCGTCG TGCCGTCGCC GACGTCTGGC TGGACAACTC GGGCAGCCCA GAGGATTTGG TGCGGCGGGC CCGCGACGTC TGGAACACGC GCGTCCAGCC CTTCGCGCAC AACCTGGCCC AACGTCAGAT TGCGCGCGCG CCGGCTAGGT TGGTGCCGGC GGATCCAAGC TGGCCGGATC AGGCGCGGCG CATCGTCAAC CGGCTAAAGA TCGCGTGCGG GCATAAGGCC TTGCGAGTTG ACCACATTGG GTCAACCGCC GTGTCGGGCT TCCCCGATTT TCTAGCCAAG GATGTCATCG ACATCCAGGT CACCGTCGAA TCACTTGACG TGGCCGACGA GCTGGCCGAG CCCTTGCTGG CCGCCGGCTA CCCACGCCTC GAGCACATCA CCCAGGACAC CGAAAAGACC GACGCTCGCA GCACCGTCGG CCGCTACGAC CACACCGACA GTGCCGCTCT GTGGCACAAG CGCGTGCACG CCTCGGCGGA TCCCGGTCGG CCGACCAACG TGCACCTGCG GGTGCACGGC TGGCCCAACC AACAGTTCGC CCTGCTGTTC GTCGACTGGC TGGCGGCCAA TCCCGGCGCG AGAGAAGACT ATTTGACGGT CAAGTGTGAC GCCGACAGGC GCGCCGACGG TGAGCTCGCG CGCTACGTCA CCGCCAAGGA GCCGTGGTTC CTGGATGCCT ACCAGCGGGC ATGGGAGTGG GCGGATGCGG TGCACTGGCG TCCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT