MytuD.19140.a

hypothetical hydrolase; membrane protein

CENTER ID: MytuD.19140.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.19140.a.B1.GE39014 full length 1 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83331.22.peg.4265
RefSeq: NP_218319.1
RefSeq: YP_006517299.1
TubercuList: Rv3802c
MTB Network Portal: Rv3802c
UniProt: O53581

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKNSRRKRH RILAWIAAGA MASVVALVIV AVVIMLRGAE SPPSAVPPGV LPPGPTPAHP HKPRPAFQDA SCPDVQMISV PGTWESSPQQ NPLNPVQFPK ALLLKVTGPI AQQFAPARVQ TYTVAYTAQF HNPLTTDNQM SYNDSRAEGT RAMVAAMTDM NNRCPLTSYV LIGFSQGAVI AGDVASDIGN GRGPVDEDLV LGVTLIADGR RQQGVGNQVP PSPRGEGAEI TLHEVPVLSG LGLTMTGPRP GGFGALDGRT NEICAQGDLI CAAPAQAFSP ANLPTTLNTL AGGAGQPVHA MYATPEFWNS DGEPATEWTL NWAHQLIENA PHPKHR
NT Sequence
atggccaaga actctcggcg taagcgccac cgaatccttg cctggatagc ggcgggcgcg atggcgtccg tcgtagcgct ggtcatcgtg gccgtggtga tcatgctgcg cggcgccgaa tcgccgccca gcgcggtgcc gccaggagtt ttaccaccgg ggcccacccc agcacatccg cacaaacccc gaccggcctt ccaggatgcg tcctgccccg acgtgcagat gatctcggtt cccggaacct gggagtcatc gccgcagcag aacccactga atccggtgca gtttccgaag gcgttgctgc tcaaggtaac tgggccgatc gcccagcaat tcgcacccgc ccgggtgcag acctacacag ttgcctacac ggcccaattc cacaatccgc tgaccacgga taatcagatg tcgtacaacg acagccgggc agagggcacc cgcgcgatgg tggcagcgat gaccgatatg aacaacaggt gcccgttgac cagctatgtg ctgatcgggt tttcgcaggg cgcggtgatc gcgggcgacg tggctagcga tatcggcaac ggccggggac ccgtggacga ggacctagta ctaggcgtga cgctgatcgc cgacggtcgt cgtcagcagg gggtgggtaa tcaggtcccg cctagcccgc gcggcgaagg cgccgagatc accctgcacg aagtcccagt gttgtccggg cttggtttga ctatgaccgg cccgcggccg ggcggttttg gtgcgctcga cggccggacc aacgagatct gcgcccaggg cgacctgatc tgtgcggccc cggcacaggc gttcagtccc gccaacttgc cgactacgct gaacaccctg gccggcggcg ccggccagcc ggtccatgct atgtatgcga cacccgaatt ctggaattcc gatggagaac cggcgaccga gtggacgttg aactgggcgc atcaacttat cgaaaatgcg ccccacccca aacataggtg a
Details for MytuD.19140.a.B1.GE39014
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM AKNSRRKRHR ILAWIAAGAM ASVVALVIVA VVIMLRGAES PPSAVPPGVL PPGPTPAHPH KPRPAFQDAS CPDVQMISVP GTWESSPQQN PLNPVQFPKA LLLKVTGPIA QQFAPARVQT YTVAYTAQFH NPLTTDNQMS YNDSRAEGTR AMVAAMTDMN NRCPLTSYVL IGFSQGAVIA GDVASDIGNG RGPVDEDLVL GVTLIADGRR QQGVGNQVPP SPRGEGAEIT LHEVPVLSGL GLTMTGPRPG GFGALDGRTN EICAQGDLIC AAPAQAFSPA NLPTTLNTLA GGAGQPVHAM YATPEFWNSD GEPATEWTLN WAHQLIENAP HPKHR
Validated NT Sequence
tcacttacct atgtttgggg tggggcgcat tttcgataag ttgatgcgcc cagttcaacg tccactcggt cgccggttct ccatcggaat tccagaattc gggtgtcgca tacatagcat ggaccggctg gccggcgccg ccggccaggg tgttcagcgt agtcggcaag ttggcgggac tgaacgcctg tgccggggcc gcacagatca ggtcgccctg ggcgcagatc tcgttggtcc ggccgtcgag cgcaccaaaa ccgcccggcc gcgggccggt catagtcaaa ccaagcccgg acaacactgg gacttcgtgc agggtgatct cggcgccttc gccgcgcggg ctaggcggga cctgattacc caccccctgc tgacgacgac cgtcggcgat cagcgtcacg cctagtacta ggtcctcgtc cacgggtccc cggccgttgc cgatatcgct agccacgtcg cccgcgatca ccgcgccctg cgaaaacccg atcagcacat agctggtcaa cgggcacctg ttgttcatat cggtcatcgc tgccaccatc gcgcgggtgc cctctgcccg gctgtcgttg tacgacatct gattatccgt ggtcagcgga ttgtggaatt gggccgtgta ggcaactgtg taggtctgca cccgggcggg tgcgaattgc tgggcgatcg gcccagttac cttgagcagc aacgccttcg gaaactgcac cggattcagt gggttctgct gcggcgatga ctcccaggtt ccgggaaccg agatcatctg cacgtcgggg caggacgcat cctggaaggc cggtcggggt ttgtgcggat gtgctggggt gggccccggt ggtaaaactc ctggcggcac cgcgctgggc ggcgattcgg cgccgcgcag catgatcacc acggccacga tgaccagcgc tacgacggac gccatcgcgc ccgccgctat ccaggcaagg attcggtggc gcttacgccg agagttcttg gccatatggt ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMA KNSRRKRHRI LAWIAAGAMA SVVALVIVAV VIMLRGAESP PSAVPPGVLP PGPTPAHPHK PRPAFQDASC PDVQMISVPG TWESSPQQNP LNPVQFPKAL LLKVTGPIAQ QFAPARVQTY TVAYTAQFHN PLTTDNQMSY NDSRAEGTRA MVAAMTDMNN RCPLTSYVLI GFSQGAVIAG DVASDIGNGR GPVDEDLVLG VTLIADGRRQ QGVGNQVPPS PRGEGAEITL HEVPVLSGLG LTMTGPRPGG FGALDGRTNE ICAQGDLICA APAQAFSPAN LPTTLNTLAG GAGQPVHAMY ATPEFWNSDG EPATEWTLNW AHQLIENAPH PKHR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca agaactctcg gcgtaagcgc caccgaatcc ttgcctggat agcggcgggc gcgatggcgt ccgtcgtagc gctggtcatc gtggccgtgg tgatcatgct gcgcggcgcc gaatcgccgc ccagcgcggt gccgccagga gttttaccac cggggcccac cccagcacat ccgcacaaac cccgaccggc cttccaggat gcgtcctgcc ccgacgtgca gatgatctcg gttcccggaa cctgggagtc atcgccgcag cagaacccac tgaatccggt gcagtttccg aaggcgttgc tgctcaaggt aactgggccg atcgcccagc aattcgcacc cgcccgggtg cagacctaca cagttgccta cacggcccaa ttccacaatc cgctgaccac ggataatcag atgtcgtaca acgacagccg ggcagagggc acccgcgcga tggtggcagc gatgaccgat atgaacaaca ggtgcccgtt gaccagctat gtgctgatcg ggttttcgca gggcgcggtg atcgcgggcg acgtggctag cgatatcggc aacggccggg gacccgtgga cgaggaccta gtactaggcg tgacgctgat cgccgacggt cgtcgtcagc agggggtggg taatcaggtc ccgcctagcc cgcgcggcga aggcgccgag atcaccctgc acgaagtccc agtgttgtcc gggcttggtt tgactatgac cggcccgcgg ccgggcggtt ttggtgcgct cgacggccgg accaacgaga tctgcgccca gggcgacctg atctgtgcgg ccccggcaca ggcgttcagt cccgccaact tgccgactac gctgaacacc ctggccggcg gcgccggcca gccggtccat gctatgtatg cgacacccga attctggaat tccgatggag aaccggcgac cgagtggacg ttgaactggg cgcatcaact tatcgaaaat gcgccccacc ccaaacatag gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat