MytuD.19107.a
Glycine--tRNA ligase (GlyRS) (Rv2357c)
| CENTER ID: | MytuD.19107.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.19107.a.A1.GE44572 | full length | 1 | 463 | ||
| MytuD.19107.a.A2.GE44573 | LT truncation | 136 | 463 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
8T5N
DEPOSITED: 6/14/2023
DETERMINATION: XRay
CLONE: MytuD.19107.a.UW1.GE44829
PROTEIN: MytuD.19107.a.UW1.PS38711
8U2P
DEPOSITED: 9/6/2023
DETERMINATION: XRay
CLONE: MytuD.19107.a.UW1.GE44829
PROTEIN: MytuD.19107.a.UW1.PS38711
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|83332.12.peg.2636 |
| TubercuList: | Rv2357c |
| MTB Network Portal: | Rv2357c |
| UniProt: | P9WFV7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHHPVAPVID TVVNLAKRRG FVYPSGEIYG GTKSAWDYGP LGVELKENIK RQWWRSVVTG RDDVVGIDSS IILPREVWVA SGHVDVFHDP LVESLITHKR YRADHLIEAY EAKHGHPPPN GLADIRDPET GEPGQWTQPR EFNMMLKTYL GPIETEEGLH YLRPETAQGI FVNFANVVTT ARKKPPFGIG QIGKSFRNEI TPGNFIFRTR EFEQMEMEFF VEPATAKEWH QYWIDNRLQW YIDLGIRREN LRLWEHPKDK LSHYSDRTVD IEYKFGFMGN PWGELEGVAN RTDFDLSTHA RHSGVDLSFY DQINDVRYTP YVIEPAAGLT RSFMAFLIDA YTEDEAPNTK GGMDKRTVLR LDPRLAPVKA AVLPLSRHAD LSPKARDLGA ELRKCWNIDF DDAGAIGRRY RRQDEVGTPF CVTVDFDSLQ DNAVTVRERD AMTQDRVAMS SVADYLAVRL KGS
NT Sequence
gtgcaccacc ccgtggcgcc cgtcatcgac accgtggtca acctagccaa acggcgaggc ttcgtctatc cgtcgggcga gatctacggc ggcacaaagt cggcgtggga ctatggtccg ctaggggttg agctcaaaga gaacatcaag cgccagtggt ggcgatcggt ggtgactggc cgcgacgacg ttgtcggaat cgattcgtcg atcatcctgc cgcgtgaggt gtgggtggct tccggtcacg tcgatgtctt tcatgacccg ctggtcgagt cgctgatcac acataagcgc taccgggccg accatctcat cgaggcctac gaagccaaac acgggcatcc gccgcctaac gggttggccg acattcgcga cccggaaacc ggcgaacccg gccagtggac gcagccacgc gagttcaaca tgatgctcaa gacctacctc ggacccatcg agaccgagga ggggctgcac tatctgcgcc cggaaaccgc gcagggcatc ttcgtgaact ttgccaacgt ggtgacaact gctcgtaaga agccgccatt cggtatcgga cagatcggca agagctttcg caacgagatc actccgggca acttcatctt ccggacccgc gagttcgagc agatggagat ggagttcttc gtcgagccgg cgactgccaa ggaatggcat cagtactgga tcgacaaccg gctgcagtgg tatatcgacc tgggtattcg tcgggagaac ctgcggttgt gggagcaccc caaagacaag ttgtcacatt actccgaccg caccgtcgac atcgagtaca agttcggctt catgggcaat ccatggggtg aactggaagg tgtggccaac cgaaccgatt ttgacctatc gacgcatgcg cggcattccg gcgtcgacct gtctttctac gaccagatca acgatgttcg gtacacgccg tacgtcatcg agccggcagc tggcttgacc cggtcattca tggcgttttt gatcgacgcg tacaccgagg acgaggcccc gaataccaag ggtgggatgg ataagcgcac ggtgctgcga ttggacccgc ggctggcgcc ggtcaaggcg gcggtgttgc cgctgtcccg gcacgcggac ctgagcccga aggcccggga cctgggcgcc gaattacgga aatgctggaa catcgatttc gatgatgccg gcgctatcgg gcggcgttac cggcgccagg acgaggtcgg taccccgttc tgtgtgacgg ttgacttcga ctcgctgcag gacaatgccg tcaccgtgcg ggagcgtgac gccatgacgc aggaccgggt tgcgatgagc agcgtggccg actatctcgc ggtgcggctc aagggcagct ag
Details for MytuD.19107.a.A1.GE44572
| HARVESTED ON: | 11/11/2021 |
| SEQUENCED ON: | 11/11/2021 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMHHPVAPVI DTVVNLAKRR GFVYPSGEIY GGTKSAWDYG PLGVELKENI KRQWWRSVVT GRDDVVGIDS SIILPREVWV ASGHVDVFHD PLVESLITHK RYRADHLIEA YEAKHGHPPP NGLADIRDPE TGEPGQWTQP REFNMMLKTY LGPIETEEGL HYLRPETAQG IFVNFANVVT TARKKPPFGI GQIGKSFRNE ITPGNFIFRT REFEQMEMEF FXEPATAKEW HQYWIDNRLQ WYIDLGIRRE NLRLWEHPKD KLSHYSDRTV DIEYKFGFMG NPWGEXEGVA NRTDFDLSTH ARHSGVDLSF YDQINDVRYT PYVIEPAAGL TRSFMAFLID AYTEDEAPNT KGGMDKRTVL RLDPRLAPVK AAVLPLSRHA DLSPKARDLG AELRKCWNID FDDAGAIGRR YRRQDEVGTP FCVTVDFDSL QDNAVTVRER DAMTQDRVAM SSVADYLAVR LKGS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcacc accccgtggc gcccgtcatc gacaccgtgg tcaacctagc caaacggcga ggcttcgtct atccgtcggg cgagatctac ggcggcacaa agtcggcgtg ggactatggt ccgctagggg ttgagctcaa agagaacatc aagcgccagt ggtggcgatc ggtggtgact ggccgcgacg acgttgtcgg aatcgattcg tcgatcatcc tgccgcgtga ggtgtgggtg gcttccggtc acgtcgatgt ctttcatgac ccgctggtcg agtcgctgat cacacataag cgctaccggg ccgaccatct catcgaggcc tacgaagcca aacacgggca tccgccgcct aacgggttgg ccgacattcg cgacccggaa accggcgaac ccggccagtg gacgcagcca cgcgagttca acatgatgct caagacctac ctcggaccca tcgagaccga ggaggggctg cactatctgc gcccggaaac cgcgcagggc atcttcgtga actttgccaa cgtggtgaca actgctcgta agaagccgcc attcggtatc ggacagatcg gcaagagctt tcgcaacgag atcactccgg gcaacttcat cttccggacc cgcgagttcg agcagatgga gatggagttc ttcgwcgagc cggcgactgc caaggaatgg catcagtact ggatcgacaa ccggctgcag tggtatatcg acctgggtat tcgtcgggag aacctgcggt tgtgggagca ccccaaagac aagttgtcac attactccga ccgcaccgtc gacatcgagt acaagttcgg cttcatgggc aatccatggg gtgaacngga aggtgtggcc aaccgaaccg attttgacct atcgacgcat gcgcggcatt ccggcgtcga cctgtctttc tacgaccaga tcaacgatgt tcggtacacg ccgtacgtca tcgagccggc agctggcttg acccggtcat tcatggcgtt tttgatcgac gcgtacaccg aggacgaggc cccgaatacc aagggtggga tggataagcg cacggtgctg cgattggacc cgcggctggc gccggtcaag gcggcggtgt tgccgctgtc ccggcacgcg gacctgagcc cgaaggcccg ggacctgggc gccgaattac ggaaatgctg gaacatcgat ttcgatgatg ccggcgctat cgggcggcgt taccggcgcc aggacgaggt cggtaccccg ttctgtgtga cggttgactt cgactcgctg caggacaatg ccgtcaccgt gcgggagcgt gacgccatga cgcaggaccg ggttgcgatg agcagcgtgg ccgactatct cgcggtgcgg ctcaagggca gctag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHHPVAPVI DTVVNLAKRR GFVYPSGEIY GGTKSAWDYG PLGVELKENI KRQWWRSVVT GRDDVVGIDS SIILPREVWV ASGHVDVFHD PLVESLITHK RYRADHLIEA YEAKHGHPPP NGLADIRDPE TGEPGQWTQP REFNMMLKTY LGPIETEEGL HYLRPETAQG IFVNFANVVT TARKKPPFGI GQIGKSFRNE ITPGNFIFRT REFEQMEMEF FVEPATAKEW HQYWIDNRLQ WYIDLGIRRE NLRLWEHPKD KLSHYSDRTV DIEYKFGFMG NPWGELEGVA NRTDFDLSTH ARHSGVDLSF YDQINDVRYT PYVIEPAAGL TRSFMAFLID AYTEDEAPNT KGGMDKRTVL RLDPRLAPVK AAVLPLSRHA DLSPKARDLG AELRKCWNID FDDAGAIGRR YRRQDEVGTP FCVTVDFDSL QDNAVTVRER DAMTQDRVAM SSVADYLAVR LKGS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gcaccacccc gtggcgcccg tcatcgacac cgtggtcaac ctagccaaac ggcgaggctt cgtctatccg tcgggcgaga tctacggcgg cacaaagtcg gcgtgggact atggtccgct aggggttgag ctcaaagaga acatcaagcg ccagtggtgg cgatcggtgg tgactggccg cgacgacgtt gtcggaatcg attcgtcgat catcctgccg cgtgaggtgt gggtggcttc cggtcacgtc gatgtctttc atgacccgct ggtcgagtcg ctgatcacac ataagcgcta ccgggccgac catctcatcg aggcctacga agccaaacac gggcatccgc cgcctaacgg gttggccgac attcgcgacc cggaaaccgg cgaacccggc cagtggacgc agccacgcga gttcaacatg atgctcaaga cctacctcgg acccatcgag accgaggagg ggctgcacta tctgcgcccg gaaaccgcgc agggcatctt cgtgaacttt gccaacgtgg tgacaactgc tcgtaagaag ccgccattcg gtatcggaca gatcggcaag agctttcgca acgagatcac tccgggcaac ttcatcttcc ggacccgcga gttcgagcag atggagatgg agttcttcgt cgagccggcg actgccaagg aatggcatca gtactggatc gacaaccggc tgcagtggta tatcgacctg ggtattcgtc gggagaacct gcggttgtgg gagcacccca aagacaagtt gtcacattac tccgaccgca ccgtcgacat cgagtacaag ttcggcttca tgggcaatcc atggggtgaa ctggaaggtg tggccaaccg aaccgatttt gacctatcga cgcatgcgcg gcattccggc gtcgacctgt ctttctacga ccagatcaac gatgttcggt acacgccgta cgtcatcgag ccggcagctg gcttgacccg gtcattcatg gcgtttttga tcgacgcgta caccgaggac gaggccccga ataccaaggg tgggatggat aagcgcacgg tgctgcgatt ggacccgcgg ctggcgccgg tcaaggcggc ggtgttgccg ctgtcccggc acgcggacct gagcccgaag gcccgggacc tgggcgccga attacggaaa tgctggaaca tcgatttcga tgatgccggc gctatcgggc ggcgttaccg gcgccaggac gaggtcggta ccccgttctg tgtgacggtt gacttcgact cgctgcagga caatgccgtc accgtgcggg agcgtgacgc catgacgcag gaccgggttg cgatgagcag cgtggccgac tatctcgcgg tgcggctcaa gggcagctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MytuD.19107.a.A2.GE44573
| HARVESTED ON: | 11/11/2021 |
| SEQUENCED ON: | 11/11/2021 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SWTQPREFNM MLKTYLGPIE TEEGLHYLRP ETAQGIFVNF ANVVTTARKK PPFGIGQIGK SFRNEITPGN FIFRTREFEQ MEMEFFVEPA TAKEWHQYWI DNRLQWYIDL GIRRENLRLW EHPKDKLSHY SDRTVDIEYK FGFMGNPWGE LEGVANRTDF DLSTHARHSG VDLSFYDQIN DVRYTPYVIE PAAGLTRSFM AFLIDAYTED EAPNTKGGMD KRTVLRLDPR LAPVKAAVLP LSRHADLSPK ARDLGAELRK CWNIDFDDAG AIGRRYRRQD EVGTPFCVTV DFDSLQDNAV TVRERDAMTQ DRVAMSSVAD YLAVRLKGS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgtggacgc agccacgcga gttcaacatg atgctcaaga cctacctcgg acccatcgag accgaggagg ggctgcacta tctgcgcccg gaaaccgcgc agggcatctt cgtgaacttt gccaacgtgg tgacaactgc tcgtaagaag ccgccattcg gtatcggaca gatcggcaag agctttcgca acgagatcac tccgggcaac ttcatcttcc ggacccgcga gttcgagcag atggagatgg agttcttcgt cgagccggcg actgccaagg aatggcatca gtactggatc gacaaccggc tgcagtggta tatcgacctg ggtattcgtc gggagaacct gcggttgtgg gagcacccca aagacaagtt gtcacattac tccgaccgca ccgtcgacat cgagtacaag ttcggcttca tgggcaatcc atggggtgaa ctggaaggtg tggccaaccg aaccgatttt gacctatcga cgcatgcgcg gcattccggc gtcgacctgt ctttctacga ccagatcaac gatgttcggt acacgccgta cgtcatcgag ccggcagctg gcttgacccg gtcattcatg gcgtttttga tcgacgcgta caccgaggac gaggccccga ataccaaggg tgggatggat aagcgcacgg tgctgcgatt ggacccgcgg ctggcgccgg tcaaggcggc ggtgttgccg ctgtcccggc acgcggacct gagcccgaag gcccgggacc tgggcgccga attacggaaa tgctggaaca tcgatttcga tgatgccggc gctatcgggc ggcgttaccg gcgccaggac gaggtcggta ccccgttctg tgtgacggtt gacttcgact cgctgcagga caatgccgtc accgtgcggg agcgtgacgc catgacgcag gaccgggttg cgatgagcag cgtggccgac tatctcgcgg tgcggctcaa gggcagctag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SWTQPREFNM MLKTYLGPIE TEEGLHYLRP ETAQGIFVNF ANVVTTARKK PPFGIGQIGK SFRNEITPGN FIFRTREFEQ MEMEFFVEPA TAKEWHQYWI DNRLQWYIDL GIRRENLRLW EHPKDKLSHY SDRTVDIEYK FGFMGNPWGE LEGVANRTDF DLSTHARHSG VDLSFYDQIN DVRYTPYVIE PAAGLTRSFM AFLIDAYTED EAPNTKGGMD KRTVLRLDPR LAPVKAAVLP LSRHADLSPK ARDLGAELRK CWNIDFDDAG AIGRRYRRQD EVGTPFCVTV DFDSLQDNAV TVRERDAMTQ DRVAMSSVAD YLAVRLKGS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgtg gacgcagcca cgcgagttca acatgatgct caagacctac ctcggaccca tcgagaccga ggaggggctg cactatctgc gcccggaaac cgcgcagggc atcttcgtga actttgccaa cgtggtgaca actgctcgta agaagccgcc attcggtatc ggacagatcg gcaagagctt tcgcaacgag atcactccgg gcaacttcat cttccggacc cgcgagttcg agcagatgga gatggagttc ttcgtcgagc cggcgactgc caaggaatgg catcagtact ggatcgacaa ccggctgcag tggtatatcg acctgggtat tcgtcgggag aacctgcggt tgtgggagca ccccaaagac aagttgtcac attactccga ccgcaccgtc gacatcgagt acaagttcgg cttcatgggc aatccatggg gtgaactgga aggtgtggcc aaccgaaccg attttgacct atcgacgcat gcgcggcatt ccggcgtcga cctgtctttc tacgaccaga tcaacgatgt tcggtacacg ccgtacgtca tcgagccggc agctggcttg acccggtcat tcatggcgtt tttgatcgac gcgtacaccg aggacgaggc cccgaatacc aagggtggga tggataagcg cacggtgctg cgattggacc cgcggctggc gccggtcaag gcggcggtgt tgccgctgtc ccggcacgcg gacctgagcc cgaaggcccg ggacctgggc gccgaattac ggaaatgctg gaacatcgat ttcgatgatg ccggcgctat cgggcggcgt taccggcgcc aggacgaggt cggtaccccg ttctgtgtga cggttgactt cgactcgctg caggacaatg ccgtcaccgt gcgggagcgt gacgccatga cgcaggaccg ggttgcgatg agcagcgtgg ccgactatct cgcggtgcgg ctcaagggca gctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa