MytuD.19095.a

hypothetical hydrolase; conserved protein

CENTER ID: MytuD.19095.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.19095.a.B1.GE39013 full length 1 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.19095.a.B1.PS37845 full length 1 420

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3699
RefSeq: NP_217828.1
RefSeq: YP_006516788.1
TubercuList: Rv3311
MTB Network Portal: Rv3311
UniProt: O53362

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVADLVPIRL SLSAGDRYTL WAPRWRDAGD EWEAFLGKDD DLYGFESVSD LVAFVRTDTE NDLVDHPAWQ DLTGAHAHNL NPAEDNQFDL VVVEELLAEK PTAESVAALA ASLAIVSAIG SVCELAAVSK FFNGNPILGT VSGGLEHFTG KAGNKRWNSI AEVIGRSWDD VLAAIDEIIS TPEVDAELSE KVAEELAEEP EGAEEVAAEV EATQDTQEAA ESDDEEADAP GDSVVLGGDR DFWLQVGIDP IQIMTGTATF YTLRCYLDDR PIFLGRNGRI SVFGSERALA RYLADEHDHD LSDLSTYDDI RTAATDGSLA VAVTDDNVYV LSGLVDDFAD GPDAVDREQL DLAVELLRDI GDYSEDSAVD KALETTRPLG QLVAYVLDPH SVGKPTAPYA AAVREWEKLE RFVESRLRRE
NT Sequence
atggttgctg acctcgtacc catccgcttg agcctgtccg ctggtgaccg ctacacgctg tgggctcctc gctggcggga tgccggcgac gagtgggagg cgttcctggg caaagacgac gacctgtatg gcttcgagag cgtctctgac ctggtcgcgt tcgtgcgcac cgacaccgag aacgacctgg tcgaccaccc ggcatggcaa gacctgaccg gagcccacgc gcacaacctc aatccggccg aagacaatca gttcgacctg gtcgtcgtcg aggaactgct ggctgagaag ccgacggcgg agtcagtggc cgcgctggcc gcctcattgg cgatcgtatc cgccatcgga tcggtgtgcg aactggcggc agtgtcgaag ttcttcaacg gcaatcccat cctgggcacg gtttccggcg ggctcgaaca cttcaccgga aaagccggca ataaacgctg gaattcgatt gccgaggtca tcggacgcag ctgggacgac gtgctcgcgg ccatcgacga gatcatcagc acccccgagg tcgacgctga gctgtcggaa aaggtcgccg aggagttggc ggaggagccc gagggcgccg aggaagtggc ggcggaggtg gaggccacgc aggacacgca ggaggcggcc gagtccgacg acgaggaagc cgacgcaccc ggtgacagtg tcgtactggg cggcgatcgg gacttctggt tgcaggtggg catcgacccg atccagatca tgacgggcac cgccaccttc tacacgcttc gctgttacct ggatgatcga ccgatcttcc tgggccgcaa tggtcggatc agtgtgtttg gctccgagcg ggcattggcc cgctatcttg ccgatgagca cgaccacgac ttgtcggacc tgagcaccta cgacgacatc cgcacggccg ccaccgacgg ctcgctggcg gttgccgtta ccgacgacaa cgtctatgtg ctcagtgggc tggtcgacga ttttgccgac gggccggacg cggtggaccg tgagcagctc gacctggccg tcgagctgct ccgcgatatc ggcgactact ccgaggacag cgcagtcgac aaggcactcg agacaacccg cccgctgggc cagctggtgg cctatgtgtt ggacccccac tcggtcggca aacccacggc cccgtatgcg gcggctgtcc gtgaatggga gaaattggaa aggttcgtgg agtcgcggct caggcgcgaa tag
Details for MytuD.19095.a.B1.GE39013
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 47kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV ADLVPIRLSL SAGDRYTLWA PRWRDAGDEW EAFLGKDDDL YGFESVSDLV AFVRTDTEND LVDHPAWQDL TGAHAHNLNP AEDNQFDLVV VEELLAEKPT AESVAALAAS LAIVSAIGSV CELAAVSKFF NGNPILGTVS GGLEHFTGKA GNKRWNSIAE VIGRSWDDVL AAIDEIISTP EVDAELSEKV AEELAEEPEG AEEVAAEVEA TQDTQEAAES DDEEADAPGD SVVLGGDRDF WLQVGIDPIQ IMTGTATFYT LRCYLDDRPI FLGRNGRISV FGSERALARY LADEHDHDLS DLSTYDDIRT AATDGSLAVA VTDDNVYVLS GLVDDFADGP DAVDREQLDL AVELLRDIGD YSEDSAVDKA LETTRPLGQL VAYVLDPHSV GKPTAPYAAA VREWEKLERF
Validated NT Sequence
acgaaccttt ccaatttctc ccattcacgg acagccgccg catacggggc cgtgggtttg ccgaccgagt gggggtccaa cacataggcc accagctggc ccagcgggcg ggttgtctcg agtgccttgt cgactgcgct gtcctcggag tagtcgccga tatcgcggag cagctcgacg gccaggtcga gctgctcacg gtccaccgcg tccggcccgt cggcaaaatc gtcgaccagc ccactgagca catagacgtt gtcgtcggta acggcaaccg ccagcgagcc gtcggtggcg gccgtgcgga tgtcgtcgta ggtgctcagg tccgacaagt cgtggtcgtg ctcatcggca agatagcggg ccaatgcccg ctcggagcca aacacactga tccgaccatt gcggcccagg aagatcggtc gatcatccag gtaacagcga agcgtgtaga aggtggcggt gcccgtcatg atctggatcg ggtcgatgcc cacctgcaac cagaagtccc gatcgccgcc cagtacgaca ctgtcaccgg gtgcgtcggc ttcctcgtcg tcggactcgg ccgcctcctg cgtgtcctgc gtggcctcca cctccgccgc cacttcctcg gcgccctcgg gctcctccgc caactcctcg gcgacctttt ccgacagctc agcgtcgacc tcgggggtgc tgatgatctc gtcgatggcc gcgagcacgt cgtcccagct gcgtccgatg acctcggcaa tcgaattcca gcgtttattg ccggcttttc cggtgaagtg ttcgagcccg ccggaaaccg tgcccaggat gggattgccg ttgaagaact tcgacactgc cgccagttcg cacaccgatc cgatggcgga tacgatcgcc aatgaggcgg ccagcgcggc cactgactcc gccgtcggct tctcagccag cagttcctcg acgacgacca ggtcgaactg attgtcttcg gccggattga ggttgtgcgc gtgggctccg gtcaggtctt gccatgccgg gtggtcgacc aggtcgttct cggtgtcggt gcgcacgaac gcgaccaggt cagagacgct ctcgaagcca tacaggtcgt cgtctttgcc caggaacgcc tcccactcgt cgccggcatc ccgccagcga ggagcccaca gcgtgtagcg gtcaccagcg gacaggctca agcggatggg tacgaggtca gcaaccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMV ADLVPIRLSL SAGDRYTLWA PRWRDAGDEW EAFLGKDDDL YGFESVSDLV AFVRTDTEND LVDHPAWQDL TGAHAHNLNP AEDNQFDLVV VEELLAEKPT AESVAALAAS LAIVSAIGSV CELAAVSKFF NGNPILGTVS GGLEHFTGKA GNKRWNSIAE VIGRSWDDVL AAIDEIISTP EVDAELSEKV AEELAEEPEG AEEVAAEVEA TQDTQEAAES DDEEADAPGD SVVLGGDRDF WLQVGIDPIQ IMTGTATFYT LRCYLDDRPI FLGRNGRISV FGSERALARY LADEHDHDLS DLSTYDDIRT AATDGSLAVA VTDDNVYVLS GLVDDFADGP DAVDREQLDL AVELLRDIGD YSEDSAVDKA LETTRPLGQL VAYVLDPHSV GKPTAPYAAA VREWEKLERF VESRLRRE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttg ctgacctcgt acccatccgc ttgagcctgt ccgctggtga ccgctacacg ctgtgggctc ctcgctggcg ggatgccggc gacgagtggg aggcgttcct gggcaaagac gacgacctgt atggcttcga gagcgtctct gacctggtcg cgttcgtgcg caccgacacc gagaacgacc tggtcgacca cccggcatgg caagacctga ccggagccca cgcgcacaac ctcaatccgg ccgaagacaa tcagttcgac ctggtcgtcg tcgaggaact gctggctgag aagccgacgg cggagtcagt ggccgcgctg gccgcctcat tggcgatcgt atccgccatc ggatcggtgt gcgaactggc ggcagtgtcg aagttcttca acggcaatcc catcctgggc acggtttccg gcgggctcga acacttcacc ggaaaagccg gcaataaacg ctggaattcg attgccgagg tcatcggacg cagctgggac gacgtgctcg cggccatcga cgagatcatc agcacccccg aggtcgacgc tgagctgtcg gaaaaggtcg ccgaggagtt ggcggaggag cccgagggcg ccgaggaagt ggcggcggag gtggaggcca cgcaggacac gcaggaggcg gccgagtccg acgacgagga agccgacgca cccggtgaca gtgtcgtact gggcggcgat cgggacttct ggttgcaggt gggcatcgac ccgatccaga tcatgacggg caccgccacc ttctacacgc ttcgctgtta cctggatgat cgaccgatct tcctgggccg caatggtcgg atcagtgtgt ttggctccga gcgggcattg gcccgctatc ttgccgatga gcacgaccac gacttgtcgg acctgagcac ctacgacgac atccgcacgg ccgccaccga cggctcgctg gcggttgccg ttaccgacga caacgtctat gtgctcagtg ggctggtcga cgattttgcc gacgggccgg acgcggtgga ccgtgagcag ctcgacctgg ccgtcgagct gctccgcgat atcggcgact actccgagga cagcgcagtc gacaaggcac tcgagacaac ccgcccgctg ggccagctgg tggcctatgt gttggacccc cactcggtcg gcaaacccac ggccccgtat gcggcggctg tccgtgaatg ggagaaattg gaaaggttcg tggagtcgcg gctcaggcgc gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.19095.a.B1.PS37845
PURIFICATION DATe: 2/17/2016
CONCENTRATION: 23.5mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV ADLVPIRLSL SAGDRYTLWA PRWRDAGDEW EAFLGKDDDL YGFESVSDLV AFVRTDTEND LVDHPAWQDL TGAHAHNLNP AEDNQFDLVV VEELLAEKPT AESVAALAAS LAIVSAIGSV CELAAVSKFF NGNPILGTVS GGLEHFTGKA GNKRWNSIAE VIGRSWDDVL AAIDEIISTP EVDAELSEKV AEELAEEPEG AEEVAAEVEA TQDTQEAAES DDEEADAPGD SVVLGGDRDF WLQVGIDPIQ IMTGTATFYT LRCYLDDRPI FLGRNGRISV FGSERALARY LADEHDHDLS DLSTYDDIRT AATDGSLAVA VTDDNVYVLS GLVDDFADGP DAVDREQLDL AVELLRDIGD YSEDSAVDKA LETTRPLGQL VAYVLDPHSV GKPTAPYAAA VREWEKLERF
Validated NT Sequence
acgaaccttt ccaatttctc ccattcacgg acagccgccg catacggggc cgtgggtttg ccgaccgagt gggggtccaa cacataggcc accagctggc ccagcgggcg ggttgtctcg agtgccttgt cgactgcgct gtcctcggag tagtcgccga tatcgcggag cagctcgacg gccaggtcga gctgctcacg gtccaccgcg tccggcccgt cggcaaaatc gtcgaccagc ccactgagca catagacgtt gtcgtcggta acggcaaccg ccagcgagcc gtcggtggcg gccgtgcgga tgtcgtcgta ggtgctcagg tccgacaagt cgtggtcgtg ctcatcggca agatagcggg ccaatgcccg ctcggagcca aacacactga tccgaccatt gcggcccagg aagatcggtc gatcatccag gtaacagcga agcgtgtaga aggtggcggt gcccgtcatg atctggatcg ggtcgatgcc cacctgcaac cagaagtccc gatcgccgcc cagtacgaca ctgtcaccgg gtgcgtcggc ttcctcgtcg tcggactcgg ccgcctcctg cgtgtcctgc gtggcctcca cctccgccgc cacttcctcg gcgccctcgg gctcctccgc caactcctcg gcgacctttt ccgacagctc agcgtcgacc tcgggggtgc tgatgatctc gtcgatggcc gcgagcacgt cgtcccagct gcgtccgatg acctcggcaa tcgaattcca gcgtttattg ccggcttttc cggtgaagtg ttcgagcccg ccggaaaccg tgcccaggat gggattgccg ttgaagaact tcgacactgc cgccagttcg cacaccgatc cgatggcgga tacgatcgcc aatgaggcgg ccagcgcggc cactgactcc gccgtcggct tctcagccag cagttcctcg acgacgacca ggtcgaactg attgtcttcg gccggattga ggttgtgcgc gtgggctccg gtcaggtctt gccatgccgg gtggtcgacc aggtcgttct cggtgtcggt gcgcacgaac gcgaccaggt cagagacgct ctcgaagcca tacaggtcgt cgtctttgcc caggaacgcc tcccactcgt cgccggcatc ccgccagcga ggagcccaca gcgtgtagcg gtcaccagcg gacaggctca agcggatggg tacgaggtca gcaaccatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMV ADLVPIRLSL SAGDRYTLWA PRWRDAGDEW EAFLGKDDDL YGFESVSDLV AFVRTDTEND LVDHPAWQDL TGAHAHNLNP AEDNQFDLVV VEELLAEKPT AESVAALAAS LAIVSAIGSV CELAAVSKFF NGNPILGTVS GGLEHFTGKA GNKRWNSIAE VIGRSWDDVL AAIDEIISTP EVDAELSEKV AEELAEEPEG AEEVAAEVEA TQDTQEAAES DDEEADAPGD SVVLGGDRDF WLQVGIDPIQ IMTGTATFYT LRCYLDDRPI FLGRNGRISV FGSERALARY LADEHDHDLS DLSTYDDIRT AATDGSLAVA VTDDNVYVLS GLVDDFADGP DAVDREQLDL AVELLRDIGD YSEDSAVDKA LETTRPLGQL VAYVLDPHSV GKPTAPYAAA VREWEKLERF VESRLRRE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTTG CTGACCTCGT ACCCATCCGC TTGAGCCTGT CCGCTGGTGA CCGCTACACG CTGTGGGCTC CTCGCTGGCG GGATGCCGGC GACGAGTGGG AGGCGTTCCT GGGCAAAGAC GACGACCTGT ATGGCTTCGA GAGCGTCTCT GACCTGGTCG CGTTCGTGCG CACCGACACC GAGAACGACC TGGTCGACCA CCCGGCATGG CAAGACCTGA CCGGAGCCCA CGCGCACAAC CTCAATCCGG CCGAAGACAA TCAGTTCGAC CTGGTCGTCG TCGAGGAACT GCTGGCTGAG AAGCCGACGG CGGAGTCAGT GGCCGCGCTG GCCGCCTCAT TGGCGATCGT ATCCGCCATC GGATCGGTGT GCGAACTGGC GGCAGTGTCG AAGTTCTTCA ACGGCAATCC CATCCTGGGC ACGGTTTCCG GCGGGCTCGA ACACTTCACC GGAAAAGCCG GCAATAAACG CTGGAATTCG ATTGCCGAGG TCATCGGACG CAGCTGGGAC GACGTGCTCG CGGCCATCGA CGAGATCATC AGCACCCCCG AGGTCGACGC TGAGCTGTCG GAAAAGGTCG CCGAGGAGTT GGCGGAGGAG CCCGAGGGCG CCGAGGAAGT GGCGGCGGAG GTGGAGGCCA CGCAGGACAC GCAGGAGGCG GCCGAGTCCG ACGACGAGGA AGCCGACGCA CCCGGTGACA GTGTCGTACT GGGCGGCGAT CGGGACTTCT GGTTGCAGGT GGGCATCGAC CCGATCCAGA TCATGACGGG CACCGCCACC TTCTACACGC TTCGCTGTTA CCTGGATGAT CGACCGATCT TCCTGGGCCG CAATGGTCGG ATCAGTGTGT TTGGCTCCGA GCGGGCATTG GCCCGCTATC TTGCCGATGA GCACGACCAC GACTTGTCGG ACCTGAGCAC CTACGACGAC ATCCGCACGG CCGCCACCGA CGGCTCGCTG GCGGTTGCCG TTACCGACGA CAACGTCTAT GTGCTCAGTG GGCTGGTCGA CGATTTTGCC GACGGGCCGG ACGCGGTGGA CCGTGAGCAG CTCGACCTGG CCGTCGAGCT GCTCCGCGAT ATCGGCGACT ACTCCGAGGA CAGCGCAGTC GACAAGGCAC TCGAGACAAC CCGCCCGCTG GGCCAGCTGG TGGCCTATGT GTTGGACCCC CACTCGGTCG GCAAACCCAC GGCCCCGTAT GCGGCGGCTG TCCGTGAATG GGAGAAATTG GAAAGGTTCG TGGAGTCGCG GCTCAGGCGC GAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT