SSGCID: Target Page

MytuD.19056.a

hypothetical hydrolase; membrane protein

CENTER ID:	MytuD.19056.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.19056.a.B1.GE39012	full length		1	421

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83331.22.peg.256
RefSeq:	NP_214741.1
RefSeq:	YP_006513549.1
TubercuList:	Rv0227c
MTB Network Portal:	Rv0227c
UniProt:	P96409

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRFAACGAI GLGAALLIAA LLLSTYTTSR IAEIPLDIDA TLISDGTGTA LDSASLATEH IVVNQDVPLV SQQQVTVESP ANADVVTLQV GSSLRRTDKQ KDSGLLLAIV DTVTLNRKTA MAVSDDTHTG GAVQKPRGLN DENPPTAIPL RHDGLSYRFP FHTEKKTYPY FDPIAQKAFD ANYEGEEDVN GLTTYRFTQN VGYTPEGKLV APLKYPSLYA GDEDGKVTTS AAMWGLPGDP NEQITMTRYY AAQRTFWVDP VSGTIVKETE RANHYFARDP LKPEVTFADY QVTSTEETVE SQVNAARDER DRLALWSRVL PITFTAAGLV ALVGGGLFAS FSLRTEGALM AASGDRDDHD YRRGGFEEPV PGAEAETEKL PTQRPDFPRE PSGSDPPRLG SAQPPPPPDA GHPDPGPPER R

NT Sequence

atgttgcggt tcgccgcgtg cggcgctatc ggactcggag ccgccctact gatcgccgcc ctgctgctgt cgacctatac caccagcagg atcgctgaga tcccactcga tatcgatgcc acgttgatca gcgacggcac tggaacggca ctcgactcgg cgtcgctagc caccgagcac atcgtggtca accaggacgt gccgctggtg tcccaacagc aagtcaccgt cgaatcgccc gccaacgccg acgtggtcac acttcaggtc ggatcctcgc tccggcgcac cgacaagcag aaggacagcg ggctgctgct ggcgatcgtc gacacggtca ccctcaaccg caagacggcg atggctgtct ccgacgacac ccacaccggc ggcgccgtcc agaaaccgcg gggcctcaat gacgagaacc cgccgacggc cattccgctg cggcacgacg ggctgtctta ccggttcccg tttcacaccg agaagaagac atacccctat ttcgacccca tcgcacagaa ggcgtttgac gccaactacg aaggcgagga ggacgtcaac ggtttaacca cataccgctt cacgcagaac gtcggctaca cccccgaggg gaagctggtg gctcccctca aatacccgtc gctgtacgcc ggcgacgagg acggcaaagt caccacatca gcggcgatgt gggggctacc cggtgatccg aacgagcaaa tcaccatgac ccgctattac gcggcgcagc gaacattctg ggtggacccg gtgtccggca ccatcgtcaa agaaaccgaa cgcgccaacc actacttcgc tcgtgaccca ctcaaaccag aggtcacgtt tgccgactac caggtcacct ccaccgaaga gacggtcgaa tcccaggtca acgcggctcg cgacgagcgc gaccggctgg cgctgtggtc gcgggtgctg ccgattacat tcaccgcggc cggcttggtc gcgctggtcg gcggtggtct gttcgcgtcg tttagccttc ggaccgaagg tgcgctgatg gctgccagcg gggatcggga cgaccacgat taccgccgcg gcggcttcga agaaccggtg cctggagcgg aggctgagac cgagaaactg cccacgcagc gccccgactt cccgcgcgag ccgagtggct cggatccgcc ccgactcggt tcggcgcagc cgcccccacc gccggacgcg gggcatcccg accccggccc gcccgagcgg cgctaa

Details for MytuD.19056.a.B1.GE39012

HARVESTED ON:	3/4/2015
SEQUENCED ON:	3/4/2015
EXPECTED MW:	47kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML RFAACGAIGL GAALLIAALL LSTYTTSRIA EIPLDIDATL ISDGTGTALD SASLATEHIV VNQDVPLVSQ QQVTVESPAN ADVVTLQVGS SLRRTDKQKD SGLLLAIVDT VTLNRKTAMA VSDDTHTGGA VQKPRGLNDE NPPTAIPLRH DGLSYRFPFH TEKKTYPYFD PIAQKAFDAN YEGEEDVNGL TTYRFTQNVG YTPEGKLVAP LKYPSLYAGD EDGKVTTSAA MWGLPGDPNE QITMTRYYAA QRTFWVDPVS GTIVKETERA NHYFARDPLK PEVTFADYQV TSTEETVESQ VNAARDERDR LALWSRVLPI TFTAAGLVAL VGGGLFASFS LRTEGALMAA SGDRDDHDYR RGGFEEPVPG AEAETEKLPT QRPDFPREPS GSDPPRLGSA QPPPPPDAGH P

Validated NT Sequence

gtcgggatgc cccgcgtccg gcggtggggg cggctgcgcc gaaccgagtc ggggcggatc cgagccactc ggctcgcgcg ggaagtcggg gcgctgcgtg ggcagtttct cggtctcagc ctccgctcca ggcaccggtt cttcgaagcc gccgcggcgg taatcgtggt cgtcccgatc cccgctggca gccatcagcg caccttcggt ccgaaggcta aacgacgcga acagaccacc gccgaccagc gcgaccaagc cggccgcggt gaatgtaatc ggcagcaccc gcgaccacag cgccagccgg tcgcgctcgt cgcgagccgc gttgacctgg gattcgaccg tctcttcggt ggaggtgacc tggtagtcgg caaacgtgac ctctggtttg agtgggtcac gagcgaagta gtggttggcg cgttcggttt ctttgacgat ggtgccggac accgggtcca cccagaatgt tcgctgcgcc gcgtaatagc gggtcatggt gatttgctcg ttcggatcac cgggtagccc ccacatcgcc gctgatgtgg tgactttgcc gtcctcgtcg ccggcgtaca gcgacgggta tttgagggga gccaccagct tcccctcggg ggtgtagccg acgttctgcg tgaagcggta tgtggttaaa ccgttgacgt cctcctcgcc ttcgtagttg gcgtcaaacg ccttctgtgc gatggggtcg aaataggggt atgtcttctt ctcggtgtga aacgggaacc ggtaagacag cccgtcgtgc cgcagcggaa tggccgtcgg cgggttctcg tcattgaggc cccgcggttt ctggacggcg ccgccggtgt gggtgtcgtc ggagacagcc atcgccgtct tgcggttgag ggtgaccgtg tcgacgatcg ccagcagcag cccgctgtcc ttctgcttgt cggtgcgccg gagcgaggat ccgacctgaa gtgtgaccac gtcggcgttg gcgggcgatt cgacggtgac ttgctgttgg gacaccagcg gcacgtcctg gttgaccacg atgtgctcgg tggctagcga cgccgagtcg agtgccgttc cagtgccgtc gctgatcaac gtggcatcga tatcgagtgg gatctcagcg atcctgctgg tggtataggt cgacagcagc agggcggcga tcagtagggc ggctccgagt ccgatagcgc cgcacgcggc gaaccgcaac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc ggttcgccgc gtgcggcgct atcggactcg gagccgccct actgatcgcc gccctgctgc tgtcgaccta taccaccagc aggatcgctg agatcccact cgatatcgat gccacgttga tcagcgacgg cactggaacg gcactcgact cggcgtcgct agccaccgag cacatcgtgg tcaaccagga cgtgccgctg gtgtcccaac agcaagtcac cgtcgaatcg cccgccaacg ccgacgtggt cacacttcag gtcggatcct cgctccggcg caccgacaag cagaaggaca gcgggctgct gctggcgatc gtcgacacgg tcaccctcaa ccgcaagacg gcgatggctg tctccgacga cacccacacc ggcggcgccg tccagaaacc gcggggcctc aatgacgaga acccgccgac ggccattccg ctgcggcacg acgggctgtc ttaccggttc ccgtttcaca ccgagaagaa gacatacccc tatttcgacc ccatcgcaca gaaggcgttt gacgccaact acgaaggcga ggaggacgtc aacggtttaa ccacataccg cttcacgcag aacgtcggct acacccccga ggggaagctg gtggctcccc tcaaataccc gtcgctgtac gccggcgacg aggacggcaa agtcaccaca tcagcggcga tgtgggggct acccggtgat ccgaacgagc aaatcaccat gacccgctat tacgcggcgc agcgaacatt ctgggtggac ccggtgtccg gcaccatcgt caaagaaacc gaacgcgcca accactactt cgctcgtgac ccactcaaac cagaggtcac gtttgccgac taccaggtca cctccaccga agagacggtc gaatcccagg tcaacgcggc tcgcgacgag cgcgaccggc tggcgctgtg gtcgcgggtg ctgccgatta cattcaccgc ggccggcttg gtcgcgctgg tcggcggtgg tctgttcgcg tcgtttagcc ttcggaccga aggtgcgctg atggctgcca gcggggatcg ggacgaccac gattaccgcc gcggcggctt cgaagaaccg gtgcctggag cggaggctga gaccgagaaa ctgcccacgc agcgccccga cttcccgcgc gagccgagtg gctcggatcc gccccgactc ggttcggcgc agccgccccc accgccggac gcggggcatc ccgaccccgg cccgcccgag cggcgctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t