SSGCID: Target Page

MytuD.18889.a

Alanine--tRNA ligase (AlaRS) (Rv2555c)

CENTER ID:	MytuD.18889.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18889.a.A1.GE44570	full length		1	904
MytuD.18889.a.A2.GE44571	LT truncation		1	436

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18889.a.A2.PW39189	LT truncation		1	436

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2862
TubercuList:	Rv2555c
MTB Network Portal:	Rv2555c
UniProt:	P9WFW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQTHEIRKRF LDHFVKAGHT EVPSASVILD DPNLLFVNAG MVQFVPFFLG QRTPPYPTAT SIQKCIRTPD IDEVGITTRH NTFFQMAGNF SFGDYFKRGA IELAWALLTN SLAAGGYGLD PERIWTTVYF DDDEAVRLWQ EVAGLPAERI QRRGMADNYW SMGIPGPCGP SSEIYYDRGP EFGPAGGPIV SEDRYLEVWN LVFMQNERGE GTTKEDYQIL GPLPRKNIDT GMGVERIALV LQDVHNVYET DLLRPVIDTV ARVAARAYDV GNHEDDVRYR IIADHSRTAA ILIGDGVSPG NDGRGYVLRR LLRRVIRSAK LLGIDAAIVG DLMATVRNAM GPSYPELVAD FERISRIAVA EETAFNRTLA SGSRLFEEVA SSTKKSGATV LSGSDAFTLH DTYGFPIELT LEMAAETGLQ VDEIGFRELM AEQRRRAKAD AAARKHAHAD LSAYRELVDA GATEFTGFDE LRSQARILGI FVDGKRVPVV AHGVAGGAGE GQRVELVLDR TPLYAESGGQ IADEGTISGT GSSEAARAAV TDVQKIAKTL WVHRVNVESG EFVEGDTVIA AVDPGWRRGA TQGHSGTHMV HAALRQVLGP NAVQAGSLNR PGYLRFDFNW QGPLTDDQRT QVEEVTNEAV QADFEVRTFT EQLDKAKAMG AIALFGESYP DEVRVVEMGG PFSLELCGGT HVSNTAQIGP VTILGESSIG SGVRRVEAYV GLDSFRHLAK ERALMAGLAS SLKVPSEEVP ARVANLVERL RAAEKELERV RMASARAAAT NAAAGAQRIG NVRLVAQRMS GGMTAADLRS LIGDIRGKLG SEPAVVALIA EGESQTVPYA VAANPAAQDL GIRANDLVKQ LAVAVEGRGG GKADLAQGSG KNPTGIDAAL DAVRSEIAVI ARVG

NT Sequence

gtgcagacac acgagatcag gaagcggttc ctcgatcatt tcgtgaaggc gggccacacc gaggtgccca gcgcctcggt gatcctcgac gaccccaacc tgttgttcgt caacgccggg atggtccagt tcgtgccttt cttcttggga cagcgcacgc cgccgtaccc gacggccacc agcatccaga agtgcatccg tacccccgat atcgacgagg tgggcataac cacccggcac aacacgtttt ttcagatggc cggcaatttc agcttcggcg actatttcaa acgcggggcc attgaactgg cctgggcact gctgaccaac agcctcgccg ccggcggcta cggcctggac ccggaaagaa tctggacgac agtctatttc gacgacgacg aagctgtccg gctatggcag gaggttgccg ggctgccggc ggagcgaatc cagcgccgcg gcatggccga caactactgg tcgatgggca ttcccggacc gtgcgggccg tcatcggaga tctattacga ccgcggaccc gaattcggtc ccgcaggcgg tcccatcgtc agcgaagacc gctacctcga ggtctggaac ctggtgttca tgcagaacga gcgcggagag ggaaccacca aggaggacta ccagatcctc gggccgctgc cccgcaagaa catcgacacc ggcatgggcg tcgagcggat cgcgctggtg ctgcaagacg tgcacaacgt ctacgagacc gacctgctca ggccggtcat cgataccgtg gccagggtcg ccgcgcgtgc ctacgacgtc ggcaaccacg aagacgacgt gcggtaccgc atcatcgcag accacagccg caccgccgcg atcctgatcg gtgacggcgt cagccccggc aacgacggtc gcggttatgt gctgcgccgg ctgctgcgtc gggtgatccg ctccgccaag ctgctgggca tcgacgctgc gatcgttggc gacctgatgg ccacggtgcg caacgcgatg ggcccgtcat atcccgaact cgtcgccgac ttcgagcgga tcagccggat cgcggtcgcc gaggagacgg cgttcaaccg cacgctggcg tcgggttcca ggctgttcga ggaggtggct agctccacca agaaatccgg agccaccgtg ctgtccggat cggacgcttt cacgttgcat gacacctacg ggttcccgat cgagctcacg ctggagatgg cggccgaaac cggtctgcag gtagacgaaa tcgggttccg tgagctgatg gccgagcagc gccgccgtgc caaggccgac gccgccgcgc gcaaacacgc gcatgctgac ctgagcgcct accgcgagct ggttgacgcc ggcgccaccg agttcaccgg attcgacgag ttgcgttccc aggcgcggat tctgggcatc ttcgtcgacg gtaagcgggt tccggtggtg gcgcacggtg tagccggcgg agccggggaa gggcagcgtg tcgaacttgt cttagatcgc accccgctct acgccgaatc gggtgggcag atcgccgatg agggcaccat cagcggaacc ggttccagcg aagctgcccg ggccgcggtt accgacgtgc agaagatcgc caaaacgctt tgggtgcacc gagtcaacgt ggaatccggg gaattcgtcg agggtgacac cgtaatcgcg gcggtggatc ccgggtggcg ccggggtgcc acgcagggcc actcgggcac ccacatggtg catgccgcgc tgcgacaagt gctggggccc aacgcggttc aggcgggatc gctgaaccgg ccgggatatt tgcgcttcga ctttaactgg cagggtccgt tgaccgacga ccagcgcacc caggtcgaag aggtcaccaa cgaggccgtg caagcggact tcgaggtgcg cacgttcacc gaacagctcg acaaggccaa ggcgatgggt gccatcgcgc tgttcggcga gagctacccc gacgaagtgc gggtggtgga gatgggtgga ccgttctcgc tggagctatg tggcggcacc catgtgagca acacggcgca gatcggtccc gtgacgatcc tgggcgagtc gtcgatcggc tccggggtgc gccgggtgga ggcctacgtg gggttggatt cgtttcgtca cctggccaag gagcgtgcgt tgatggccgg gttggcctcg tcactgaagg tgccgtccga agaggtaccg gcccgggtgg ccaatctagt ggagcgcctg cgggccgccg agaaggaact cgaacgtgtc cggatggcca gcgcccgggc agccgccacc aatgccgccg ccggggctca gcggatcggt aacgtccgtt tggtggcgca gcgaatgtcc ggcgggatga ccgcggcaga cctgcggtcg ttgatcggcg acatccgcgg caagctgggt agcgagccgg cggtggtggc gctgattgcc gagggcgaaa gccaaactgt gccgtatgcg gtcgcggcca atcccgctgc ccaggacctc ggaatccgtg ccaacgacct ggtcaaacaa cttgcggtgg cggtcgaagg ccgcggtggc ggtaaggcgg acctggcgca gggctcggga aagaatccga ccggtatcga cgccgcgctc gacgcggtcc gctccgagat cgccgtgata gcgcgggtcg gttga

Details for MytuD.18889.a.A1.GE44570

HARVESTED ON:	11/11/2021
SEQUENCED ON:	11/11/2021
EXPECTED MW:	100kDa
OBSERVED MW:	100kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	64

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVQTHEIRK RFLDHFVKAG HTEVPSASVI LDDPNLLFVN AGMVQFVPFF LGQRTPPYPT ATSIQKCIRT PDIDEVGITT RHNTFFQMAG NFSFGDYFKR GAIELAWALL TNSLAAGGYG LDPERIWTTV YFDDDEAVRL WQEVAGLPAE RIQRRGMADN YWSMGIPGPC GPSSEIYYDR GPEFGPAGGP IVSEDRYLEV WNLVFMQNER GEGTTKEDYQ ILGPLPRKNI DTGMGVERIA LVLQDVHNVY ETXLLRPVID TVARVAARAY DVXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXAGSL XXXGYLRFDF NXQGPLTDDQ RTQVEEVTNE AVQADFEVRT FTEQLDKXKA MGAIALFGES YPDEVRVVEM GGPFSLELCG GTHVSNTAQI GPVTILGESS IGSGVRRVEA YVGLDSFRHL AKERALMAGL ASSLKVPSEE VPARVANLVE RLRAAEKELE RVRMASARAA ATNAAAGAQR IGNVRLVAQR MSGGMTAADL RSLIGDIRGK LGSEPAVVAL IAEGESQTVP YAVAANPAAQ DLGIRANDLV KQLAVAVEGR GGGKADLAQG SGKNPTGIDA ALDAVRSEIA VIARVG

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc agacacacga gatcaggaag cggttcctcg atcatttcgt gaaggcgggc cacaccgagg tgcccagcgc ctcggtgatc ctcgacgacc ccaacctgtt gttcgtcaac gccgggatgg tccagttcgt gcctttcttc ttgggacagc gcacgccgcc gtacccgacg gccaccagca tccagaagtg catccgtacc cccgatatcg acgaggtggg cataaccacc cggcacaaca cgttttttca gatggccggc aatttcagct tcggcgacta tttcaaacgc ggggccattg aactggcctg ggcactgctg accaacagcc tcgccgccgg cggctacggc ctggacccgg aaagaatctg gacgacagtc tatttcgacg acgacgaagc tgtccggcta tggcaggagg ttgccgggct gccggcggag cgaatccagc gccgcggcat ggccgacaac tactggtcga tgggcattcc cggaccgtgc gggccgtcat cggagatcta ttacgaccgc ggacccgaat tcggtcccgc aggcggtccc atcgtcagcg aagaccgcta cctcgaggtc tggaacctgg tgttcatgca gaacgagcgc ggagagggaa ccaccaagga ggactaccag atcctcgggc cgctgccccg caagaacatc gacaccggca tgggcgtcga gcggatcgcg ctggtgctgc aagacgtgca caacgtctac gagaccganc tgctcaggcc ggtcatcgat accgtggcca gggtcgccgc gcgtgcctac gacgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggc gggatcgctg anccnncngg gatatttgcg cttcgacttt aactnncagg gtccgttgac cgacgaccag cgcacccagg tcgaagaggt caccaacgar gccgtgcaag cggacttcga ggtgcgcacg ttcacngaac agctcgacaa gnccaaggcg atgggtgcca tcgcgctgtt cggcgagagc taccccgacg aagtgcgggt ggtggagatg ggtggaccgt tctcgctgga gctatgtggc ggcacccatg tgagcaacac ggcgcagatc ggtcccgtga cgatcctggg cgagtcgtcg atcggctccg gggtgcgccg ggtggaggcc tacgtggggt tggattcgtt tcgtcacctg gccaaggagc gtgcgttgat ggccgggttg gcctcgtcac tgaaggtgcc gtccgaagag gtaccggccc gggtggccaa tctagtggag cgcctgcggg ccgccgagaa ggaactcgaa cgtgtccgga tggccagcgc ccgggcagcc gccaccaatg ccgccgccgg ggctcagcgg atcggtaacg tccgtttggt ggcgcagcga atgtccggcg ggatgaccgc ggcagacctg cggtcgttga tcggcgacat ccgcggcaag ctgggtagcg agccggcggt ggtggcgctg attgccgagg gcgaaagcca aactgtgccg tatgcggtcg cggccaatcc cgctgcccag gacctcggaa tccgtgccaa cgacctggtc aaacaacttg cggtggcggt cgaaggccgc ggtggcggta aggcggacct ggcgcagggc tcgggaaaga atccgaccgg tatcgacgcc gcgctcgacg cggtccgctc cgagatcgcc gtgatagcgc gggtcggttg a

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQTHEIRKR FLDHFVKAGH TEVPSASVIL DDPNLLFVNA GMVQFVPFFL GQRTPPYPTA TSIQKCIRTP DIDEVGITTR HNTFFQMAGN FSFGDYFKRG AIELAWALLT NSLAAGGYGL DPERIWTTVY FDDDEAVRLW QEVAGLPAER IQRRGMADNY WSMGIPGPCG PSSEIYYDRG PEFGPAGGPI VSEDRYLEVW NLVFMQNERG EGTTKEDYQI LGPLPRKNID TGMGVERIAL VLQDVHNVYE TDLLRPVIDT VARVAARAYD VGNHEDDVRY RIIADHSRTA AILIGDGVSP GNDGRGYVLR RLLRRVIRSA KLLGIDAAIV GDLMATVRNA MGPSYPELVA DFERISRIAV AEETAFNRTL ASGSRLFEEV ASSTKKSGAT VLSGSDAFTL HDTYGFPIEL TLEMAAETGL QVDEIGFREL MAEQRRRAKA DAAARKHAHA DLSAYRELVD AGATEFTGFD ELRSQARILG IFVDGKRVPV VAHGVAGGAG EGQRVELVLD RTPLYAESGG QIADEGTISG TGSSEAARAA VTDVQKIAKT LWVHRVNVES GEFVEGDTVI AAVDPGWRRG ATQGHSGTHM VHAALRQVLG PNAVQAGSLN RPGYLRFDFN WQGPLTDDQR TQVEEVTNEA VQADFEVRTF TEQLDKAKAM GAIALFGESY PDEVRVVEMG GPFSLELCGG THVSNTAQIG PVTILGESSI GSGVRRVEAY VGLDSFRHLA KERALMAGLA SSLKVPSEEV PARVANLVER LRAAEKELER VRMASARAAA TNAAAGAQRI GNVRLVAQRM SGGMTAADLR SLIGDIRGKL GSEPAVVALI AEGESQTVPY AVAANPAAQD LGIRANDLVK QLAVAVEGRG GGKADLAQGS GKNPTGIDAA LDAVRSEIAV IARVG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gcagacacac gagatcagga agcggttcct cgatcatttc gtgaaggcgg gccacaccga ggtgcccagc gcctcggtga tcctcgacga ccccaacctg ttgttcgtca acgccgggat ggtccagttc gtgcctttct tcttgggaca gcgcacgccg ccgtacccga cggccaccag catccagaag tgcatccgta cccccgatat cgacgaggtg ggcataacca cccggcacaa cacgtttttt cagatggccg gcaatttcag cttcggcgac tatttcaaac gcggggccat tgaactggcc tgggcactgc tgaccaacag cctcgccgcc ggcggctacg gcctggaccc ggaaagaatc tggacgacag tctatttcga cgacgacgaa gctgtccggc tatggcagga ggttgccggg ctgccggcgg agcgaatcca gcgccgcggc atggccgaca actactggtc gatgggcatt cccggaccgt gcgggccgtc atcggagatc tattacgacc gcggacccga attcggtccc gcaggcggtc ccatcgtcag cgaagaccgc tacctcgagg tctggaacct ggtgttcatg cagaacgagc gcggagaggg aaccaccaag gaggactacc agatcctcgg gccgctgccc cgcaagaaca tcgacaccgg catgggcgtc gagcggatcg cgctggtgct gcaagacgtg cacaacgtct acgagaccga cctgctcagg ccggtcatcg ataccgtggc cagggtcgcc gcgcgtgcct acgacgtcgg caaccacgaa gacgacgtgc ggtaccgcat catcgcagac cacagccgca ccgccgcgat cctgatcggt gacggcgtca gccccggcaa cgacggtcgc ggttatgtgc tgcgccggct gctgcgtcgg gtgatccgct ccgccaagct gctgggcatc gacgctgcga tcgttggcga cctgatggcc acggtgcgca acgcgatggg cccgtcatat cccgaactcg tcgccgactt cgagcggatc agccggatcg cggtcgccga ggagacggcg ttcaaccgca cgctggcgtc gggttccagg ctgttcgagg aggtggctag ctccaccaag aaatccggag ccaccgtgct gtccggatcg gacgctttca cgttgcatga cacctacggg ttcccgatcg agctcacgct ggagatggcg gccgaaaccg gtctgcaggt agacgaaatc gggttccgtg agctgatggc cgagcagcgc cgccgtgcca aggccgacgc cgccgcgcgc aaacacgcgc atgctgacct gagcgcctac cgcgagctgg ttgacgccgg cgccaccgag ttcaccggat tcgacgagtt gcgttcccag gcgcggattc tgggcatctt cgtcgacggt aagcgggttc cggtggtggc gcacggtgta gccggcggag ccggggaagg gcagcgtgtc gaacttgtct tagatcgcac cccgctctac gccgaatcgg gtgggcagat cgccgatgag ggcaccatca gcggaaccgg ttccagcgaa gctgcccggg ccgcggttac cgacgtgcag aagatcgcca aaacgctttg ggtgcaccga gtcaacgtgg aatccgggga attcgtcgag ggtgacaccg taatcgcggc ggtggatccc gggtggcgcc ggggtgccac gcagggccac tcgggcaccc acatggtgca tgccgcgctg cgacaagtgc tggggcccaa cgcggttcag gcgggatcgc tgaaccggcc gggatatttg cgcttcgact ttaactggca gggtccgttg accgacgacc agcgcaccca ggtcgaagag gtcaccaacg aggccgtgca agcggacttc gaggtgcgca cgttcaccga acagctcgac aaggccaagg cgatgggtgc catcgcgctg ttcggcgaga gctaccccga cgaagtgcgg gtggtggaga tgggtggacc gttctcgctg gagctatgtg gcggcaccca tgtgagcaac acggcgcaga tcggtcccgt gacgatcctg ggcgagtcgt cgatcggctc cggggtgcgc cgggtggagg cctacgtggg gttggattcg tttcgtcacc tggccaagga gcgtgcgttg atggccgggt tggcctcgtc actgaaggtg ccgtccgaag aggtaccggc ccgggtggcc aatctagtgg agcgcctgcg ggccgccgag aaggaactcg aacgtgtccg gatggccagc gcccgggcag ccgccaccaa tgccgccgcc ggggctcagc ggatcggtaa cgtccgtttg gtggcgcagc gaatgtccgg cgggatgacc gcggcagacc tgcggtcgtt gatcggcgac atccgcggca agctgggtag cgagccggcg gtggtggcgc tgattgccga gggcgaaagc caaactgtgc cgtatgcggt cgcggccaat cccgctgccc aggacctcgg aatccgtgcc aacgacctgg tcaaacaact tgcggtggcg gtcgaaggcc gcggtggcgg taaggcggac ctggcgcagg gctcgggaaa gaatccgacc ggtatcgacg ccgcgctcga cgcggtccgc tccgagatcg ccgtgatagc gcgggtcggt taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for MytuD.18889.a.A2.GE44571

HARVESTED ON:	11/11/2021
SEQUENCED ON:	11/11/2021
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

Validated NT Sequence

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gcagacacac gagatcagga agcggttcct cgatcatttc gtgaaggcgg gccacaccga ggtgcccagc gcctcggtga tcctcgacga ccccaacctg ttgttcgtca acgccgggat ggtccagttc gtgcctttct tcttgggaca gcgcacgccg ccgtacccga cggccaccag catccagaag tgcatccgta cccccgatat cgacgaggtg ggcataacca cccggcacaa cacgtttttt cagatggccg gcaatttcag cttcggcgac tatttcaaac gcggggccat tgaactggcc tgggcactgc tgaccaacag cctcgccgcc ggcggctacg gcctggaccc ggaaagaatc tggacgacag tctatttcga cgacgacgaa gctgtccggc tatggcagga ggttgccggg ctgccggcgg agcgaatcca gcgccgcggc atggccgaca actactggtc gatgggcatt cccggaccgt gcgggccgtc atcggagatc tattacgacc gcggacccga attcggtccc gcaggcggtc ccatcgtcag cgaagaccgc tacctcgagg tctggaacct ggtgttcatg cagaacgagc gcggagaggg aaccaccaag gaggactacc agatcctcgg gccgctgccc cgcaagaaca tcgacaccgg catgggcgtc gagcggatcg cgctggtgct gcaagacgtg cacaacgtct acgagaccga cctgctcagg ccggtcatcg ataccgtggc cagggtcgcc gcgcgtgcct acgacgtcgg caaccacgaa gacgacgtgc ggtaccgcat catcgcagac cacagccgca ccgccgcgat cctgatcggt gacggcgtca gccccggcaa cgacggtcgc ggttatgtgc tgcgccggct gctgcgtcgg gtgatccgct ccgccaagct gctgggcatc gacgctgcga tcgttggcga cctgatggcc acggtgcgca acgcgatggg cccgtcatat cccgaactcg tcgccgactt cgagcggatc agccggatcg cggtcgccga ggagacggcg ttcaaccgca cgctggcgtc gggttccagg ctgttcgagg aggtggctag ctccaccaag aaatccggag ccaccgtgct gtccggatcg gacgctttca cgttgcatga cacctacggg ttcccgatcg agctcacgct ggagatggcg gccgaaaccg gtctgcaggt agacgaaatc gggttccgtg agctgatggc cgagcagcgc cgccgttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MytuD.18889.a.A2.PW39189

PURIFICATION DATe:	1/25/2023
CONCENTRATION:	15.6mg/ml
OBSERVED MW:	51kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	9
VIAL VOLUME:	70µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGT GCAGACACAC GAGATCAGGA AGCGGTTCCT CGATCATTTC GTGAAGGCGG GCCACACCGA GGTGCCCAGC GCCTCGGTGA TCCTCGACGA CCCCAACCTG TTGTTCGTCA ACGCCGGGAT GGTCCAGTTC GTGCCTTTCT TCTTGGGACA GCGCACGCCG CCGTACCCGA CGGCCACCAG CATCCAGAAG TGCATCCGTA CCCCCGATAT CGACGAGGTG GGCATAACCA CCCGGCACAA CACGTTTTTT CAGATGGCCG GCAATTTCAG CTTCGGCGAC TATTTCAAAC GCGGGGCCAT TGAACTGGCC TGGGCACTGC TGACCAACAG CCTCGCCGCC GGCGGCTACG GCCTGGACCC GGAAAGAATC TGGACGACAG TCTATTTCGA CGACGACGAA GCTGTCCGGC TATGGCAGGA GGTTGCCGGG CTGCCGGCGG AGCGAATCCA GCGCCGCGGC ATGGCCGACA ACTACTGGTC GATGGGCATT CCCGGACCGT GCGGGCCGTC ATCGGAGATC TATTACGACC GCGGACCCGA ATTCGGTCCC GCAGGCGGTC CCATCGTCAG CGAAGACCGC TACCTCGAGG TCTGGAACCT GGTGTTCATG CAGAACGAGC GCGGAGAGGG AACCACCAAG GAGGACTACC AGATCCTCGG GCCGCTGCCC CGCAAGAACA TCGACACCGG CATGGGCGTC GAGCGGATCG CGCTGGTGCT GCAAGACGTG CACAACGTCT ACGAGACCGA CCTGCTCAGG CCGGTCATCG ATACCGTGGC CAGGGTCGCC GCGCGTGCCT ACGACGTCGG CAACCACGAA GACGACGTGC GGTACCGCAT CATCGCAGAC CACAGCCGCA CCGCCGCGAT CCTGATCGGT GACGGCGTCA GCCCCGGCAA CGACGGTCGC GGTTATGTGC TGCGCCGGCT GCTGCGTCGG GTGATCCGCT CCGCCAAGCT GCTGGGCATC GACGCTGCGA TCGTTGGCGA CCTGATGGCC ACGGTGCGCA ACGCGATGGG CCCGTCATAT CCCGAACTCG TCGCCGACTT CGAGCGGATC AGCCGGATCG CGGTCGCCGA GGAGACGGCG TTCAACCGCA CGCTGGCGTC GGGTTCCAGG CTGTTCGAGG AGGTGGCTAG CTCCACCAAG AAATCCGGAG CCACCGTGCT GTCCGGATCG GACGCTTTCA CGTTGCATGA CACCTACGGG TTCCCGATCG AGCTCACGCT GGAGATGGCG GCCGAAACCG GTCTGCAGGT AGACGAAATC GGGTTCCGTG AGCTGATGGC CGAGCAGCGC CGCCGTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA