SSGCID: Target Page

MytuD.18849.a

Valine--tRNA ligase (ValRS) (Rv2448c)

CENTER ID:	MytuD.18849.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18849.a.A1.GE44568	full length		1	886
MytuD.18849.a.A2.GE44569	LT truncation		44	886

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
MytuD.18849.a.A1.PW39188	full length	1	886
MytuD.18849.a.A3.PW39219	truncation #2	8	886
MytuD.18849.a.A5.PW39220	AlphaFold model	11	886

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2744
TubercuList:	Rv2448c
MTB Network Portal:	Rv2448c
UniProt:	P9WFS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTASPHPAAD MLPKSWDPAA MESAIYQKWL DAGYFTADPT STKPAYSIVL PPPNVTGSLH MGHALEHTMM DALTRRKRMQ GYEVLWQPGT DHAGIATQSV VEQQLAVDGK TKEDLGRELF VDKVWDWKRE SGGAIGGQMR RLGDGVDWSR DRFTMDEGLS RAVRTIFKRL YDAGLIYRAE RLVNWSPVLQ TAISDLEVNY RDVEGELVSF RYGSLDDSQP HIVVATTRVE TMLGDTAIAV HPDDERYRHL VGTSLAHPFV DRELAIVADE HVDPEFGTGA VKVTPAHDPN DFEIGVRHQL PMPSILDTKG RIVDTGTRFD GMDRFEARVA VRQALAAQGR VVEEKRPYLH SVGHSERSGE PIEPRLSLQW WVRVESLAKA AGDAVRNGDT VIHPASMEPR WFSWVDDMHD WCISRQLWWG HRIPIWYGPD GEQVCVGPDE TPPQGWEQDP DVLDTWFSSA LWPFSTLGWP DKTAELEKFY PTSVLVTGYD ILFFWVARMM MFGTFVGDDA AITLDGRRGP QVPFTDVFLH GLIRDESGRK MSKSKGNVID PLDWVEMFGA DALRFTLARG ASPGGDLAVS EDAVRASRNF GTKLFNATRY ALLNGAAPAP LPSPNELTDA DRWILGRLEE VRAEVDSAFD GYEFSRACES LYHFAWDEFC DWYLELAKTQ LAQGLTHTTA VLAAGLDTLL RLLHPVIPFL TEALWLALTG RESLVSADWP EPSGISVDLV AAQRINDMQK LVTEVRRFRS DQGLADRQKV PARMHGVRDS DLSNQVAAVT SLAWLTEPGP DFEPSVSLEV RLGPEMNRTV VVELDTSGTI DVAAERRRLE KELAGAQKEL ASTAAKLANA DFLAKAPDAV IAKIRDRQRV AQQETERITT RLAALQ

NT Sequence

gtgaccgcca gccctcaccc tgccgccgat atgctgccca agtcgtggga tccggccgcg atggagagcg ccatctatca gaagtggctg gacgctggct acttcaccgc ggacccgacc agcaccaagc cggcctattc gatcgtgctg ccgccgccga acgtgaccgg cagcctgcac atgggccacg cgctggaaca caccatgatg gacgccttga cgcggcgcaa gcggatgcag ggctatgagg tgctctggca gccgggcacc gaccatgccg ggatcgccac ccagagcgtg gtcgagcagc agctggcggt cgacggcaag actaaagaag acctcggccg cgagctgttc gtggacaagg tgtgggattg gaagcgagag tctggcggtg ccatcggcgg ccagatgcgc cgactcggtg acggggtgga ctggagccgc gaccggttca ccatggacga aggtctgtcg cgggcggtgc gcacgatctt caagcggctt tatgacgccg ggctgatcta tcgggccgag cggctggtca actggtcgcc ggtgctgcag accgcgatct ccgacctcga ggtcaactac cgcgacgtcg aaggcgagct ggtgtcgttt aggtacggct cgcttgacga ctcgcaaccc cacatcgtgg tcgccaccac ccgggtcgag acgatgctgg gcgataccgc gatcgccgtc catcccgatg acgagcgcta ccgtcacctg gtcggcacca gcctggcgca cccattcgtc gaccgggagc tggccattgt cgccgacgag cacgtggacc ctgaattcgg caccggcgcg gtcaaagtca cacccgccca cgaccccaac gacttcgaaa tcggggtgcg ccaccagctg ccgatgccct cgatcctgga caccaagggc cggatcgtcg acaccggaac gcgattcgac ggcatggacc gcttcgaggc acgggtcgcg gtgcgccaag cgctcgcggc ccagggccgc gtggtcgaag aaaagcgacc ctacctgcac agcgtcggac actccgaacg cagcggcgag ccgatcgagc cgcggctatc cctgcagtgg tgggtccggg tggaatcgct ggccaaagcg gccggggatg cggtgcgcaa cggggacacc gtgattcacc cggccagcat ggaaccccgc tggttctcct gggtcgacga catgcacgac tggtgcatct cgcgacagct ctggtggggg catcggatcc cgatctggta cggacccgac ggcgaacagg tgtgcgtcgg cccggacgaa acacccccgc agggctggga acaggatcct gacgtgctgg atacctggtt ttcgtcggcg ctgtggccgt tttccacgct gggttggccg gacaagacgg cggagctgga aaagttctat ccgacaagcg ttctggttac cggctatgac atcttgttct tttgggtggc cagaatgatg atgttcggca ccttcgtcgg cgacgacgcc gccatcaccc tcgacggccg ccggggcccg caggtgccgt tcaccgacgt gtttctgcat gggctgatcc gcgacgagtc tggccgcaag atgagcaagt ccaagggcaa cgtcatcgac ccgctggatt gggtggaaat gttcggggcc gatgcgctgc ggttcacgct ggcccgcggg gccagtcccg gtggtgactt ggcggtgagc gaggatgccg tgcgggcgtc gcgcaatttc gggaccaagc tgttcaacgc cactcggtac gcactgctca atggcgccgc gccagcaccc ctgccatcgc cgaacgagct gaccgacgcc gaccgctgga ttctcggaag gttggaagag gttcgggccg aagttgattc ggccttcgac ggatacgagt tcagccgcgc ttgtgagtcc ctgtatcact tcgcctggga cgaattctgc gactggtacc tcgaactggc caaaacgcag cttgcccagg gactcacaca caccaccgcc gtgctggccg ccgggctgga cacgctgctg cgcctgctgc acccggtgat tcccttcctc accgaggcgc tatggctggc gctgaccggc agggaatcgc tggtcagcgc cgactggccg gagccttccg ggattagcgt ggaccttgtt gccgcgcaac ggattaacga tatgcagaag ttggtgaccg aagtgcggcg gttccgcagc gatcaaggtc tggccgaccg gcagaaggtt ccggcccgaa tgcacggtgt gcgggactcg gatctgagca accaggtggc cgccgtgacc tcgctggcgt ggctcaccga gccgggcccg gattttgagc cgtcggtctc gttggaggtt cggctcggcc ccgagatgaa ccgcaccgtc gtcgtcgagc tcgacacctc gggcaccatc gacgtggccg ccgagcgtcg ccgcctggaa aaggagttgg ccggcgccca aaaggagctg gcgtcgaccg ccgccaagtt ggccaacgcg gactttctgg ccaaagcgcc cgacgccgtc attgccaaga tccgggaccg ccagcgcgtg gcgcagcagg aaaccgagcg catcaccacc cggttggctg cgctgcaatg a

Details for MytuD.18849.a.A1.GE44568

HARVESTED ON:	11/11/2021
SEQUENCED ON:	11/11/2021
EXPECTED MW:	101kDa
OBSERVED MW:	101kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	68

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLPKSWDPA AMESAIYQKW LDAGYFTADP TSTKPAYSIV LPPPNVTGSL HMGHALEHTM MDALTRRKRM QGYEVLWQPG TDHAGIATQS VVEQQLAVDG KTKEDLGREL FVDKVWDWKR ESGGAIGGQM RRLGDGVDWS RDRFTMDEGL SRAVRTIFKR LYDAGLIYRA ERLVNWSPVL QTAISDLEVN YRDVEGELVS FRYGSLDDSQ PHIVVATTRV ETMLGDTAIA VHPDDERYRH LVGTSLAHPF VDRELAIVAD EHVDPEFGTG AVKVTPAHDP NDFEIGVRXQ LPMPXILXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX SXDAVRASRN FGXKLFNATR YALLNGAAPA PLPSPNELTD ADRWILGRXE EVRAEVDSAF DGYEFSRACE SLYHFAWDEF CDWYLELAKT QLAQGLTHTT AVLAAGLDTL LRLLHPVIPF LTEALWLALT GRESLVSADW PEPSGISVDL VAAQRINDMQ KLVTEVRRFR SDQGLADRQK VPARMHGVRD SDLSNQVAAV TSLAWLTEPG PDFEPSVSLE VRLGPEMNRT VVVELDTSGT IDVAAERRRL EKELAGAQKE LASTAAKLAN ADFLAKAPDA VIAKIRDRQR VAQQETERIT TRLAALQ

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctgc ccaagtcgtg ggatccggcc gcgatggaga gcgccatcta tcagaagtgg ctggacgctg gctacttcac cgcggacccg accagcacca agccggccta ttcgatcgtg ctgccgccgc cgaacgtgac cggcagcctg cacatgggcc acgcgctgga acacaccatg atggacgcct tgacgcggcg caagcggatg cagggctatg aggtgctctg gcagccgggc accgaccatg ccgggatcgc cacccagagc gtggtcgagc agcagctggc ggtcgacggc aagactaaag aagacctcgg ccgcgagctg ttcgtggaca aggtgtggga ttggaagcga gagtctggcg gtgccatcgg cggccagatg cgccgactcg gtgacggggt ggactggagc cgcgaccggt tcaccatgga cgaaggtctg tcgcgggcgg tgcgcacgat cttcaagcgg ctttatgacg ccgggctgat ctatcgggcc gagcggctgg tcaactggtc gccggtgctg cagaccgcga tctccgacct cgaggtcaac taccgcgacg tcgaaggcga gctggtgtcg tttaggtacg gctcgcttga cgactcgcaa ccccacatcg tggtcgccac cacccgggtc gagacgatgc tgggcgatac cgcgatcgcc gtccatcccg atgacgagcg ctaccgtcac ctggtcggca ccagcctggc gcacccattc gtcgaccggg agctggccat tgtcgccgac gagcacgtgg accctgaatt cggcaccggc gcggtcaaag tcacacccgc ccacgacccc aacgacttcg aaatcggggt gcgccancag ctgccgatgc ccncgatcct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcgnggatg ccgtgcgggc gtcgcgcaat ttcgggncca agctgttcaa cgccactcgg tacgcactgc tcaatggcgc cgcgccagca cccctgccat cgccgaacga gctgaccgac gccgaccgct ggattctcgg aaggtnggaa gaggttcggg ccgaagttga ttcggccttc gacggatacg agttcagccg cgcttgtgag tccctgtatc acttcgcctg ggacgaattc tgcgactggt acctcgaact ggccaaaacg cagcttgccc agggactcac acacaccacc gccgtgctgg ccgccgggct ggacacgctg ctgcgcctgc tgcacccggt gattcccttc ctcaccgagg cgctatggct ggcgctgacc ggcagggaat cgctggtcag cgccgactgg ccggagcctt ccgggattag cgtggacctt gttgccgcgc aacggattaa cgatatgcag aagttggtga ccgaagtgcg gcggttccgc agcgatcaag gtctggccga ccggcagaag gttccggccc gaatgcacgg tgtgcgggac tcggatctga gcaaccaggt ggccgccgtg acctcgctgg cgtggctcac cgagccgggc ccggattttg agccgtcggt ctcgttggag gttcggctcg gccccgagat gaaccgcacc gtcgtcgtcg agctcgacac ctcgggcacc atcgacgtgg ccgccgagcg tcgccgcctg gaaaaggagt tggccggcgc ccaaaaggag ctggcgtcga ccgccgccaa gttggccaac gcggactttc tggccaaagc gcccgacgcc gtcattgcca agatccggga ccgccagcgc gtggcgcagc aggaaaccga gcgcatcacc acccggttgg ctgcgctgca atga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTASPHPAA DMLPKSWDPA AMESAIYQKW LDAGYFTADP TSTKPAYSIV LPPPNVTGSL HMGHALEHTM MDALTRRKRM QGYEVLWQPG TDHAGIATQS VVEQQLAVDG KTKEDLGREL FVDKVWDWKR ESGGAIGGQM RRLGDGVDWS RDRFTMDEGL SRAVRTIFKR LYDAGLIYRA ERLVNWSPVL QTAISDLEVN YRDVEGELVS FRYGSLDDSQ PHIVVATTRV ETMLGDTAIA VHPDDERYRH LVGTSLAHPF VDRELAIVAD EHVDPEFGTG AVKVTPAHDP NDFEIGVRHQ LPMPSILDTK GRIVDTGTRF DGMDRFEARV AVRQALAAQG RVVEEKRPYL HSVGHSERSG EPIEPRLSLQ WWVRVESLAK AAGDAVRNGD TVIHPASMEP RWFSWVDDMH DWCISRQLWW GHRIPIWYGP DGEQVCVGPD ETPPQGWEQD PDVLDTWFSS ALWPFSTLGW PDKTAELEKF YPTSVLVTGY DILFFWVARM MMFGTFVGDD AAITLDGRRG PQVPFTDVFL HGLIRDESGR KMSKSKGNVI DPLDWVEMFG ADALRFTLAR GASPGGDLAV SEDAVRASRN FGTKLFNATR YALLNGAAPA PLPSPNELTD ADRWILGRLE EVRAEVDSAF DGYEFSRACE SLYHFAWDEF CDWYLELAKT QLAQGLTHTT AVLAAGLDTL LRLLHPVIPF LTEALWLALT GRESLVSADW PEPSGISVDL VAAQRINDMQ KLVTEVRRFR SDQGLADRQK VPARMHGVRD SDLSNQVAAV TSLAWLTEPG PDFEPSVSLE VRLGPEMNRT VVVELDTSGT IDVAAERRRL EKELAGAQKE LASTAAKLAN ADFLAKAPDA VIAKIRDRQR VAQQETERIT TRLAALQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gaccgccagc cctcaccctg ccgccgatat gctgcccaag tcgtgggatc cggccgcgat ggagagcgcc atctatcaga agtggctgga cgctggctac ttcaccgcgg acccgaccag caccaagccg gcctattcga tcgtgctgcc gccgccgaac gtgaccggca gcctgcacat gggccacgcg ctggaacaca ccatgatgga cgccttgacg cggcgcaagc ggatgcaggg ctatgaggtg ctctggcagc cgggcaccga ccatgccggg atcgccaccc agagcgtggt cgagcagcag ctggcggtcg acggcaagac taaagaagac ctcggccgcg agctgttcgt ggacaaggtg tgggattgga agcgagagtc tggcggtgcc atcggcggcc agatgcgccg actcggtgac ggggtggact ggagccgcga ccggttcacc atggacgaag gtctgtcgcg ggcggtgcgc acgatcttca agcggcttta tgacgccggg ctgatctatc gggccgagcg gctggtcaac tggtcgccgg tgctgcagac cgcgatctcc gacctcgagg tcaactaccg cgacgtcgaa ggcgagctgg tgtcgtttag gtacggctcg cttgacgact cgcaacccca catcgtggtc gccaccaccc gggtcgagac gatgctgggc gataccgcga tcgccgtcca tcccgatgac gagcgctacc gtcacctggt cggcaccagc ctggcgcacc cattcgtcga ccgggagctg gccattgtcg ccgacgagca cgtggaccct gaattcggca ccggcgcggt caaagtcaca cccgcccacg accccaacga cttcgaaatc ggggtgcgcc accagctgcc gatgccctcg atcctggaca ccaagggccg gatcgtcgac accggaacgc gattcgacgg catggaccgc ttcgaggcac gggtcgcggt gcgccaagcg ctcgcggccc agggccgcgt ggtcgaagaa aagcgaccct acctgcacag cgtcggacac tccgaacgca gcggcgagcc gatcgagccg cggctatccc tgcagtggtg ggtccgggtg gaatcgctgg ccaaagcggc cggggatgcg gtgcgcaacg gggacaccgt gattcacccg gccagcatgg aaccccgctg gttctcctgg gtcgacgaca tgcacgactg gtgcatctcg cgacagctct ggtgggggca tcggatcccg atctggtacg gacccgacgg cgaacaggtg tgcgtcggcc cggacgaaac acccccgcag ggctgggaac aggatcctga cgtgctggat acctggtttt cgtcggcgct gtggccgttt tccacgctgg gttggccgga caagacggcg gagctggaaa agttctatcc gacaagcgtt ctggttaccg gctatgacat cttgttcttt tgggtggcca gaatgatgat gttcggcacc ttcgtcggcg acgacgccgc catcaccctc gacggccgcc ggggcccgca ggtgccgttc accgacgtgt ttctgcatgg gctgatccgc gacgagtctg gccgcaagat gagcaagtcc aagggcaacg tcatcgaccc gctggattgg gtggaaatgt tcggggccga tgcgctgcgg ttcacgctgg cccgcggggc cagtcccggt ggtgacttgg cggtgagcga ggatgccgtg cgggcgtcgc gcaatttcgg gaccaagctg ttcaacgcca ctcggtacgc actgctcaat ggcgccgcgc cagcacccct gccatcgccg aacgagctga ccgacgccga ccgctggatt ctcggaaggt tggaagaggt tcgggccgaa gttgattcgg ccttcgacgg atacgagttc agccgcgctt gtgagtccct gtatcacttc gcctgggacg aattctgcga ctggtacctc gaactggcca aaacgcagct tgcccaggga ctcacacaca ccaccgccgt gctggccgcc gggctggaca cgctgctgcg cctgctgcac ccggtgattc ccttcctcac cgaggcgcta tggctggcgc tgaccggcag ggaatcgctg gtcagcgccg actggccgga gccttccggg attagcgtgg accttgttgc cgcgcaacgg attaacgata tgcagaagtt ggtgaccgaa gtgcggcggt tccgcagcga tcaaggtctg gccgaccggc agaaggttcc ggcccgaatg cacggtgtgc gggactcgga tctgagcaac caggtggccg ccgtgacctc gctggcgtgg ctcaccgagc cgggcccgga ttttgagccg tcggtctcgt tggaggttcg gctcggcccc gagatgaacc gcaccgtcgt cgtcgagctc gacacctcgg gcaccatcga cgtggccgcc gagcgtcgcc gcctggaaaa ggagttggcc ggcgcccaaa aggagctggc gtcgaccgcc gccaagttgg ccaacgcgga ctttctggcc aaagcgcccg acgccgtcat tgccaagatc cgggaccgcc agcgcgtggc gcagcaggaa accgagcgca tcaccacccg gttggctgcg ctgcaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MytuD.18849.a.A2.GE44569

HARVESTED ON:	11/11/2021
SEQUENCED ON:	11/11/2021
EXPECTED MW:	96kDa
OBSERVED MW:	96kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	67

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SPAYSIVLPP PNVTGSLHMG HALEHTMMDA LTRRKRMQGY EVLWQPGTDH AGIATQSVVE QQLAVDGKTK EDLGRELFVD KVWDWKRESG GAIGGQMRRL GDGVDWSRDR FTMDEGLSRA VRTIFKRLYD AGLIYRAERL VNWSPVLQTA ISDLEVNYRD VEGELVSFRY GSLDDSQPHI VVATTRVETM LGDTAIAVHP DDERYRHLVG TSLAHPFVDR ELAIVADEHV DPEFGTGAVK VTPAHDPNDF EIGVRHQLPM PSILXTXGRI VDTGTRFDGM XRFEAXVAVR QALAXQGRVX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXLXRLEEVR AEVDSAFDGY EFSRACESLY HFAWDEFCDW YLELAKTQLA QGLTHTTAVL AAGLDTLLRL LHPVIPFLTE ALWLALTGRE SLVSADWPEP SGISVDLVAA QRINDMQKLV TEVRRFRSDQ GLADRQKVPA RMHGVRDSDL SNQVAAVTSL AWLTEPGPDF EPSVSLEVRL GPEMNRTVVV ELDTSGTIDV AAERRRLEKE LAGAQKELAS TAAKLANADF LAKAPDAVIA KIRDRQRVAQ QETERITTRL AALQ

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgccggcct attcgatcgt gctgccgccg ccgaacgtga ccggcagcct gcacatgggc cacgcgctgg aacacaccat gatggacgcc ttgacgcggc gcaagcggat gcagggctat gaggtgctct ggcagccggg caccgaccat gccgggatcg ccacccagag cgtggtcgag cagcagctgg cggtcgacgg caagactaaa gaagacctcg gccgcgagct gttcgtggac aaggtgtggg attggaagcg agagtctggc ggtgccatcg gcggccagat gcgccgactc ggtgacgggg tggactggag ccgcgaccgg ttcaccatgg acgaaggtct gtcgcgggcg gtgcgcacga tcttcaagcg gctttatgac gccgggctga tctatcgggc cgagcggctg gtcaactggt cgccggtgct gcagaccgcg atctccgacc tcgaggtcaa ctaccgcgac gtcgaaggcg agctggtgtc gtttaggtac ggctcgcttg acgactcgca accccacatc gtggtcgcca ccacccgggt cgagacgatg ctgggcgata ccgcgatcgc cgtccatccc gatgacgagc gctaccgtca cctggtcggc accagcctgg cgcacccatt cgtcgaccgg gagctggcca ttgtcgccga cgagcacgtg gaccctgaat tcggcaccgg cgcggtcaaa gtcacacccg cccacgaccc caacgacttc gaaatcgggg tgcgccacca gctgccgatg ccctcgatcc tggncaccna gggccggatc gtcgacaccg gaacgcgatt cgacggcatg gnccgcttcg aggcangggt cgcggtgcgc caagcgctcg cggnccaggg ccgcgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttctcg naaggttgga agaggttcgg gcngaagttg attcggcntt cgacggatac gagttcagcc gcgcttgtga gtccctgtat cacttcgcct gggacgaatt ctgcgactgg tacctcgaac tggccaaaac gcagcttgcc cagggactca cacacaccac cgccgtgctg gccgccgggc tggacacgct gctgcgcctg ctgcacccgg tgattccctt cctcaccgag gcgctatggc tggcgctgac cggcagggaa tcgctggtca gcgccgactg gccggagcct tccgggatta gcgtggacct tgttgccgcg caacggatta acgatatgca gaagttggtg accgaagtgc ggcggttccg cagcgatcaa ggtctggccg accggcagaa ggttccggcc cgaatgcacg gtgtgcggga ctcggatctg agcaaccagg tggccgccgt gacctcgctg gcgtggctca ccgagccggg cccggatttt gagccgtcgg tctcgttgga ggttcggctc ggccccgaga tgaaccgcac cgtcgtcgtc gagctcgaca cctcgggcac catcgacgtg gccgccgagc gtcgccgcct ggaaaaggag ttggccggcg cccaaaagga gctggcgtcg accgccgcca agttggccaa cgcggacttt ctggccaaag cgcccgacgc cgtcattgcc aagatccggg accgccagcg cgtggcgcag caggaaaccg agcgcatcac cacccggttg gctgcgctgc aatga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SPAYSIVLPP PNVTGSLHMG HALEHTMMDA LTRRKRMQGY EVLWQPGTDH AGIATQSVVE QQLAVDGKTK EDLGRELFVD KVWDWKRESG GAIGGQMRRL GDGVDWSRDR FTMDEGLSRA VRTIFKRLYD AGLIYRAERL VNWSPVLQTA ISDLEVNYRD VEGELVSFRY GSLDDSQPHI VVATTRVETM LGDTAIAVHP DDERYRHLVG TSLAHPFVDR ELAIVADEHV DPEFGTGAVK VTPAHDPNDF EIGVRHQLPM PSILDTKGRI VDTGTRFDGM DRFEARVAVR QALAAQGRVV EEKRPYLHSV GHSERSGEPI EPRLSLQWWV RVESLAKAAG DAVRNGDTVI HPASMEPRWF SWVDDMHDWC ISRQLWWGHR IPIWYGPDGE QVCVGPDETP PQGWEQDPDV LDTWFSSALW PFSTLGWPDK TAELEKFYPT SVLVTGYDIL FFWVARMMMF GTFVGDDAAI TLDGRRGPQV PFTDVFLHGL IRDESGRKMS KSKGNVIDPL DWVEMFGADA LRFTLARGAS PGGDLAVSED AVRASRNFGT KLFNATRYAL LNGAAPAPLP SPNELTDADR WILGRLEEVR AEVDSAFDGY EFSRACESLY HFAWDEFCDW YLELAKTQLA QGLTHTTAVL AAGLDTLLRL LHPVIPFLTE ALWLALTGRE SLVSADWPEP SGISVDLVAA QRINDMQKLV TEVRRFRSDQ GLADRQKVPA RMHGVRDSDL SNQVAAVTSL AWLTEPGPDF EPSVSLEVRL GPEMNRTVVV ELDTSGTIDV AAERRRLEKE LAGAQKELAS TAAKLANADF LAKAPDAVIA KIRDRQRVAQ QETERITTRL AALQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgcc ggcctattcg atcgtgctgc cgccgccgaa cgtgaccggc agcctgcaca tgggccacgc gctggaacac accatgatgg acgccttgac gcggcgcaag cggatgcagg gctatgaggt gctctggcag ccgggcaccg accatgccgg gatcgccacc cagagcgtgg tcgagcagca gctggcggtc gacggcaaga ctaaagaaga cctcggccgc gagctgttcg tggacaaggt gtgggattgg aagcgagagt ctggcggtgc catcggcggc cagatgcgcc gactcggtga cggggtggac tggagccgcg accggttcac catggacgaa ggtctgtcgc gggcggtgcg cacgatcttc aagcggcttt atgacgccgg gctgatctat cgggccgagc ggctggtcaa ctggtcgccg gtgctgcaga ccgcgatctc cgacctcgag gtcaactacc gcgacgtcga aggcgagctg gtgtcgttta ggtacggctc gcttgacgac tcgcaacccc acatcgtggt cgccaccacc cgggtcgaga cgatgctggg cgataccgcg atcgccgtcc atcccgatga cgagcgctac cgtcacctgg tcggcaccag cctggcgcac ccattcgtcg accgggagct ggccattgtc gccgacgagc acgtggaccc tgaattcggc accggcgcgg tcaaagtcac acccgcccac gaccccaacg acttcgaaat cggggtgcgc caccagctgc cgatgccctc gatcctggac accaagggcc ggatcgtcga caccggaacg cgattcgacg gcatggaccg cttcgaggca cgggtcgcgg tgcgccaagc gctcgcggcc cagggccgcg tggtcgaaga aaagcgaccc tacctgcaca gcgtcggaca ctccgaacgc agcggcgagc cgatcgagcc gcggctatcc ctgcagtggt gggtccgggt ggaatcgctg gccaaagcgg ccggggatgc ggtgcgcaac ggggacaccg tgattcaccc ggccagcatg gaaccccgct ggttctcctg ggtcgacgac atgcacgact ggtgcatctc gcgacagctc tggtgggggc atcggatccc gatctggtac ggacccgacg gcgaacaggt gtgcgtcggc ccggacgaaa cacccccgca gggctgggaa caggatcctg acgtgctgga tacctggttt tcgtcggcgc tgtggccgtt ttccacgctg ggttggccgg acaagacggc ggagctggaa aagttctatc cgacaagcgt tctggttacc ggctatgaca tcttgttctt ttgggtggcc agaatgatga tgttcggcac cttcgtcggc gacgacgccg ccatcaccct cgacggccgc cggggcccgc aggtgccgtt caccgacgtg tttctgcatg ggctgatccg cgacgagtct ggccgcaaga tgagcaagtc caagggcaac gtcatcgacc cgctggattg ggtggaaatg ttcggggccg atgcgctgcg gttcacgctg gcccgcgggg ccagtcccgg tggtgacttg gcggtgagcg aggatgccgt gcgggcgtcg cgcaatttcg ggaccaagct gttcaacgcc actcggtacg cactgctcaa tggcgccgcg ccagcacccc tgccatcgcc gaacgagctg accgacgccg accgctggat tctcggaagg ttggaagagg ttcgggccga agttgattcg gccttcgacg gatacgagtt cagccgcgct tgtgagtccc tgtatcactt cgcctgggac gaattctgcg actggtacct cgaactggcc aaaacgcagc ttgcccaggg actcacacac accaccgccg tgctggccgc cgggctggac acgctgctgc gcctgctgca cccggtgatt cccttcctca ccgaggcgct atggctggcg ctgaccggca gggaatcgct ggtcagcgcc gactggccgg agccttccgg gattagcgtg gaccttgttg ccgcgcaacg gattaacgat atgcagaagt tggtgaccga agtgcggcgg ttccgcagcg atcaaggtct ggccgaccgg cagaaggttc cggcccgaat gcacggtgtg cgggactcgg atctgagcaa ccaggtggcc gccgtgacct cgctggcgtg gctcaccgag ccgggcccgg attttgagcc gtcggtctcg ttggaggttc ggctcggccc cgagatgaac cgcaccgtcg tcgtcgagct cgacacctcg ggcaccatcg acgtggccgc cgagcgtcgc cgcctggaaa aggagttggc cggcgcccaa aaggagctgg cgtcgaccgc cgccaagttg gccaacgcgg actttctggc caaagcgccc gacgccgtca ttgccaagat ccgggaccgc cagcgcgtgg cgcagcagga aaccgagcgc atcaccaccc ggttggctgc gctgcaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MytuD.18849.a.A1.PW39188

PURIFICATION DATe:	1/20/2023
CONCENTRATION:	15.05mg/ml
OBSERVED MW:	101kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	19
VIAL VOLUME:	110µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	68

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGT GACCGCCAGC CCTCACCCTG CCGCCGATAT GCTGCCCAAG TCGTGGGATC CGGCCGCGAT GGAGAGCGCC ATCTATCAGA AGTGGCTGGA CGCTGGCTAC TTCACCGCGG ACCCGACCAG CACCAAGCCG GCCTATTCGA TCGTGCTGCC GCCGCCGAAC GTGACCGGCA GCCTGCACAT GGGCCACGCG CTGGAACACA CCATGATGGA CGCCTTGACG CGGCGCAAGC GGATGCAGGG CTATGAGGTG CTCTGGCAGC CGGGCACCGA CCATGCCGGG ATCGCCACCC AGAGCGTGGT CGAGCAGCAG CTGGCGGTCG ACGGCAAGAC TAAAGAAGAC CTCGGCCGCG AGCTGTTCGT GGACAAGGTG TGGGATTGGA AGCGAGAGTC TGGCGGTGCC ATCGGCGGCC AGATGCGCCG ACTCGGTGAC GGGGTGGACT GGAGCCGCGA CCGGTTCACC ATGGACGAAG GTCTGTCGCG GGCGGTGCGC ACGATCTTCA AGCGGCTTTA TGACGCCGGG CTGATCTATC GGGCCGAGCG GCTGGTCAAC TGGTCGCCGG TGCTGCAGAC CGCGATCTCC GACCTCGAGG TCAACTACCG CGACGTCGAA GGCGAGCTGG TGTCGTTTAG GTACGGCTCG CTTGACGACT CGCAACCCCA CATCGTGGTC GCCACCACCC GGGTCGAGAC GATGCTGGGC GATACCGCGA TCGCCGTCCA TCCCGATGAC GAGCGCTACC GTCACCTGGT CGGCACCAGC CTGGCGCACC CATTCGTCGA CCGGGAGCTG GCCATTGTCG CCGACGAGCA CGTGGACCCT GAATTCGGCA CCGGCGCGGT CAAAGTCACA CCCGCCCACG ACCCCAACGA CTTCGAAATC GGGGTGCGCC ACCAGCTGCC GATGCCCTCG ATCCTGGACA CCAAGGGCCG GATCGTCGAC ACCGGAACGC GATTCGACGG CATGGACCGC TTCGAGGCAC GGGTCGCGGT GCGCCAAGCG CTCGCGGCCC AGGGCCGCGT GGTCGAAGAA AAGCGACCCT ACCTGCACAG CGTCGGACAC TCCGAACGCA GCGGCGAGCC GATCGAGCCG CGGCTATCCC TGCAGTGGTG GGTCCGGGTG GAATCGCTGG CCAAAGCGGC CGGGGATGCG GTGCGCAACG GGGACACCGT GATTCACCCG GCCAGCATGG AACCCCGCTG GTTCTCCTGG GTCGACGACA TGCACGACTG GTGCATCTCG CGACAGCTCT GGTGGGGGCA TCGGATCCCG ATCTGGTACG GACCCGACGG CGAACAGGTG TGCGTCGGCC CGGACGAAAC ACCCCCGCAG GGCTGGGAAC AGGATCCTGA CGTGCTGGAT ACCTGGTTTT CGTCGGCGCT GTGGCCGTTT TCCACGCTGG GTTGGCCGGA CAAGACGGCG GAGCTGGAAA AGTTCTATCC GACAAGCGTT CTGGTTACCG GCTATGACAT CTTGTTCTTT TGGGTGGCCA GAATGATGAT GTTCGGCACC TTCGTCGGCG ACGACGCCGC CATCACCCTC GACGGCCGCC GGGGCCCGCA GGTGCCGTTC ACCGACGTGT TTCTGCATGG GCTGATCCGC GACGAGTCTG GCCGCAAGAT GAGCAAGTCC AAGGGCAACG TCATCGACCC GCTGGATTGG GTGGAAATGT TCGGGGCCGA TGCGCTGCGG TTCACGCTGG CCCGCGGGGC CAGTCCCGGT GGTGACTTGG CGGTGAGCGA GGATGCCGTG CGGGCGTCGC GCAATTTCGG GACCAAGCTG TTCAACGCCA CTCGGTACGC ACTGCTCAAT GGCGCCGCGC CAGCACCCCT GCCATCGCCG AACGAGCTGA CCGACGCCGA CCGCTGGATT CTCGGAAGGT TGGAAGAGGT TCGGGCCGAA GTTGATTCGG CCTTCGACGG ATACGAGTTC AGCCGCGCTT GTGAGTCCCT GTATCACTTC GCCTGGGACG AATTCTGCGA CTGGTACCTC GAACTGGCCA AAACGCAGCT TGCCCAGGGA CTCACACACA CCACCGCCGT GCTGGCCGCC GGGCTGGACA CGCTGCTGCG CCTGCTGCAC CCGGTGATTC CCTTCCTCAC CGAGGCGCTA TGGCTGGCGC TGACCGGCAG GGAATCGCTG GTCAGCGCCG ACTGGCCGGA GCCTTCCGGG ATTAGCGTGG ACCTTGTTGC CGCGCAACGG ATTAACGATA TGCAGAAGTT GGTGACCGAA GTGCGGCGGT TCCGCAGCGA TCAAGGTCTG GCCGACCGGC AGAAGGTTCC GGCCCGAATG CACGGTGTGC GGGACTCGGA TCTGAGCAAC CAGGTGGCCG CCGTGACCTC GCTGGCGTGG CTCACCGAGC CGGGCCCGGA TTTTGAGCCG TCGGTCTCGT TGGAGGTTCG GCTCGGCCCC GAGATGAACC GCACCGTCGT CGTCGAGCTC GACACCTCGG GCACCATCGA CGTGGCCGCC GAGCGTCGCC GCCTGGAAAA GGAGTTGGCC GGCGCCCAAA AGGAGCTGGC GTCGACCGCC GCCAAGTTGG CCAACGCGGA CTTTCTGGCC AAAGCGCCCG ACGCCGTCAT TGCCAAGATC CGGGACCGCC AGCGCGTGGC GCAGCAGGAA ACCGAGCGCA TCACCACCCG GTTGGCTGCG CTGCAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA

Details for MytuD.18849.a.A3.PW39219

PURIFICATION DATe:	7/11/2023
CONCENTRATION:	16.3mg/ml
OBSERVED MW:	100kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SAADMLPKSW DPAAMESAIY QKWLDAGYFT ADPTSTKPAY SIVLPPPNVT GSLHMGHALE HTMMDALTRR KRMQGYEVLW QPGTDHAGIA TQSVVEQQLA VDGKTKEDLG RELFVDKVWD WKRESGGAIG GQMRRLGDGV DWSRDRFTMD EGLSRAVRTI FKRLYDAGLI YRAERLVNWS PVLQTAISDL EVNYRDVEGE LVSFRYGSLD DSQPHIVVAT TRVETMLGDT AIAVHPDDER YRHLVGTSLA HPFVDRELAI VADEHVDPEF GTGAVKVTPA HDPNDFEIGV RHQLPMPSIL DTKGRIVDTG TRFDGMDRFE ARVAVRQALA AQGRVVEEKR PYLHSVGHSE RSGEPIEPRL SLQWWVRVES LAKAAGDAVR NGDTVIHPAS MEPRWFSWVD DMHDWCISRQ LWWGHRIPIW YGPDGEQVCV GPDETPPQGW EQDPDVLDTW FSSALWPFST LGWPDKTAEL EKFYPTSVLV TGYDILFFWV ARMMMFGTFV GDDAAITLDG RRGPQVPFTD VFLHGLIRDE SGRKMSKSKG NVIDPLDWVE MFGADALRFT LARGASPGGD LAVSEDAVRA SRNFGTKLFN ATRYALLNGA APAPLPSPNE LTDADRWILG RLEEVRAEVD SAFDGYEFSR ACESLYHFAW DEFCDWYLEL AKTQLAQGLT HTTAVLAAGL DTLLRLLHPV IPFLTEALWL ALTGRESLVS ADWPEPSGIS VDLVAAQRIN DMQKLVTEVR RFRSDQGLAD RQKVPARMHG VRDSDLSNQV AAVTSLAWLT EPGPDFEPSV SLEVRLGPEM NRTVVVELDT SGTIDVAAER RRLEKELAGA QKELASTAAK LANADFLAKA PDAVIAKIRD RQRVAQQETE RITTRLAALQ

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGC CGCCGATATG CTGCCCAAGT CGTGGGATCC GGCCGCGATG GAGAGCGCCA TCTATCAGAA GTGGCTGGAC GCTGGCTACT TCACCGCGGA CCCGACCAGC ACCAAGCCGG CCTATTCGAT CGTGCTGCCG CCGCCGAACG TGACCGGCAG CCTGCACATG GGCCACGCGC TGGAACACAC CATGATGGAC GCCTTGACGC GGCGCAAGCG GATGCAGGGC TATGAGGTGC TCTGGCAGCC GGGCACCGAC CATGCCGGGA TCGCCACCCA GAGCGTGGTC GAGCAGCAGC TGGCGGTCGA CGGCAAGACT AAAGAAGACC TCGGCCGCGA GCTGTTCGTG GACAAGGTGT GGGATTGGAA GCGAGAGTCT GGCGGTGCCA TCGGCGGCCA GATGCGCCGA CTCGGTGACG GGGTGGACTG GAGCCGCGAC CGGTTCACCA TGGACGAAGG TCTGTCGCGG GCGGTGCGCA CGATCTTCAA GCGGCTTTAT GACGCCGGGC TGATCTATCG GGCCGAGCGG CTGGTCAACT GGTCGCCGGT GCTGCAGACC GCGATCTCCG ACCTCGAGGT CAACTACCGC GACGTCGAAG GCGAGCTGGT GTCGTTTAGG TACGGCTCGC TTGACGACTC GCAACCCCAC ATCGTGGTCG CCACCACCCG GGTCGAGACG ATGCTGGGCG ATACCGCGAT CGCCGTCCAT CCCGATGACG AGCGCTACCG TCACCTGGTC GGCACCAGCC TGGCGCACCC ATTCGTCGAC CGGGAGCTGG CCATTGTCGC CGACGAGCAC GTGGACCCTG AATTCGGCAC CGGCGCGGTC AAAGTCACAC CCGCCCACGA CCCCAACGAC TTCGAAATCG GGGTGCGCCA CCAGCTGCCG ATGCCCTCGA TCCTGGACAC CAAGGGCCGG ATCGTCGACA CCGGAACGCG ATTCGACGGC ATGGACCGCT TCGAGGCACG GGTCGCGGTG CGCCAAGCGC TCGCGGCCCA GGGCCGCGTG GTCGAAGAAA AGCGACCCTA CCTGCACAGC GTCGGACACT CCGAACGCAG CGGCGAGCCG ATCGAGCCGC GGCTATCCCT GCAGTGGTGG GTCCGGGTGG AATCGCTGGC CAAAGCGGCC GGGGATGCGG TGCGCAACGG GGACACCGTG ATTCACCCGG CCAGCATGGA ACCCCGCTGG TTCTCCTGGG TCGACGACAT GCACGACTGG TGCATCTCGC GACAGCTCTG GTGGGGGCAT CGGATCCCGA TCTGGTACGG ACCCGACGGC GAACAGGTGT GCGTCGGCCC GGACGAAACA CCCCCGCAGG GCTGGGAACA GGATCCTGAC GTGCTGGATA CCTGGTTTTC GTCGGCGCTG TGGCCGTTTT CCACGCTGGG TTGGCCGGAC AAGACGGCGG AGCTGGAAAA GTTCTATCCG ACAAGCGTTC TGGTTACCGG CTATGACATC TTGTTCTTTT GGGTGGCCAG AATGATGATG TTCGGCACCT TCGTCGGCGA CGACGCCGCC ATCACCCTCG ACGGCCGCCG GGGCCCGCAG GTGCCGTTCA CCGACGTGTT TCTGCATGGG CTGATCCGCG ACGAGTCTGG CCGCAAGATG AGCAAGTCCA AGGGCAACGT CATCGACCCG CTGGATTGGG TGGAAATGTT CGGGGCCGAT GCGCTGCGGT TCACGCTGGC CCGCGGGGCC AGTCCCGGTG GTGACTTGGC GGTGAGCGAG GATGCCGTGC GGGCGTCGCG CAATTTCGGG ACCAAGCTGT TCAACGCCAC TCGGTACGCA CTGCTCAATG GCGCCGCGCC AGCACCCCTG CCATCGCCGA ACGAGCTGAC CGACGCCGAC CGCTGGATTC TCGGAAGGTT GGAAGAGGTT CGGGCCGAAG TTGATTCGGC CTTCGACGGA TACGAGTTCA GCCGCGCTTG TGAGTCCCTG TATCACTTCG CCTGGGACGA ATTCTGCGAC TGGTACCTCG AACTGGCCAA AACGCAGCTT GCCCAGGGAC TCACACACAC CACCGCCGTG CTGGCCGCCG GGCTGGACAC GCTGCTGCGC CTGCTGCACC CGGTGATTCC CTTCCTCACC GAGGCGCTAT GGCTGGCGCT GACCGGCAGG GAATCGCTGG TCAGCGCCGA CTGGCCGGAG CCTTCCGGGA TTAGCGTGGA CCTTGTTGCC GCGCAACGGA TTAACGATAT GCAGAAGTTG GTGACCGAAG TGCGGCGGTT CCGCAGCGAT CAAGGTCTGG CCGACCGGCA GAAGGTTCCG GCCCGAATGC ACGGTGTGCG GGACTCGGAT CTGAGCAACC AGGTGGCCGC CGTGACCTCG CTGGCGTGGC TCACCGAGCC GGGCCCGGAT TTTGAGCCGT CGGTCTCGTT GGAGGTTCGG CTCGGCCCCG AGATGAACCG CACCGTCGTC GTCGAGCTCG ACACCTCGGG CACCATCGAC GTGGCCGCCG AGCGTCGCCG CCTGGAAAAG GAGTTGGCCG GCGCCCAAAA GGAGCTGGCG TCGACCGCCG CCAAGTTGGC CAACGCGGAC TTTCTGGCCA AAGCGCCCGA CGCCGTCATT GCCAAGATCC GGGACCGCCA GCGCGTGGCG CAGCAGGAAA CCGAGCGCAT CACCACCCGG TTGGCTGCGC TGCAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA

Details for MytuD.18849.a.A5.PW39220

PURIFICATION DATe:	7/11/2023
CONCENTRATION:	19.4mg/ml
OBSERVED MW:	100kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLPKSWDPA AMESAIYQKW LDAGYFTADP TSTKPAYSIV LPPPNVTGSL HMGHALEHTM MDALTRRKRM QGYEVLWQPG TDHAGIATQS VVEQQLAVDG KTKEDLGREL FVDKVWDWKR ESGGAIGGQM RRLGDGVDWS RDRFTMDEGL SRAVRTIFKR LYDAGLIYRA ERLVNWSPVL QTAISDLEVN YRDVEGELVS FRYGSLDDSQ PHIVVATTRV ETMLGDTAIA VHPDDERYRH LVGTSLAHPF VDRELAIVAD EHVDPEFGTG AVKVTPAHDP NDFEIGVRHQ LPMPSILDTK GRIVDTGTRF DGMDRFEARV AVRQALAAQG RVVEEKRPYL HSVGHSERSG EPIEPRLSLQ WWVRVESLAK AAGDAVRNGD TVIHPASMEP RWFSWVDDMH DWCISRQLWW GHRIPIWYGP DGEQVCVGPD ETPPQGWEQD PDVLDTWFSS ALWPFSTLGW PDKTAELEKF YPTSVLVTGY DILFFWVARM MMFGTFVGDD AAITLDGRRG PQVPFTDVFL HGLIRDESGR KMSKSKGNVI DPLDWVEMFG ADALRFTLAR GASPGGDLAV SEDAVRASRN FGTKLFNATR YALLNGAAPA PLPSPNELTD ADRWILGRLE EVRAEVDSAF DGYEFSRACE SLYHFAWDEF CDWYLELAKT QLAQGLTHTT AVLAAGLDTL LRLLHPVIPF LTEALWLALT GRESLVSADW PEPSGISVDL VAAQRINDMQ KLVTEVRRFR SDQGLADRQK VPARMHGVRD SDLSNQVAAV TSLAWLTEPG PDFEPSVSLE VRLGPEMNRT VVVELDTSGT IDVAAERRRL EKELAGAQKE LASTAAKLAN ADFLAKAPDA VIAKIRDRQR VAQQETERIT TRLAALQ

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTGCCCAAG TCGTGGGATC CGGCCGCGAT GGAGAGCGCC ATCTATCAGA AGTGGCTGGA CGCTGGCTAC TTCACCGCGG ACCCGACCAG CACCAAGCCG GCCTATTCGA TCGTGCTGCC GCCGCCGAAC GTGACCGGCA GCCTGCACAT GGGCCACGCG CTGGAACACA CCATGATGGA CGCCTTGACG CGGCGCAAGC GGATGCAGGG CTATGAGGTG CTCTGGCAGC CGGGCACCGA CCATGCCGGG ATCGCCACCC AGAGCGTGGT CGAGCAGCAG CTGGCGGTCG ACGGCAAGAC TAAAGAAGAC CTCGGCCGCG AGCTGTTCGT GGACAAGGTG TGGGATTGGA AGCGAGAGTC TGGCGGTGCC ATCGGCGGCC AGATGCGCCG ACTCGGTGAC GGGGTGGACT GGAGCCGCGA CCGGTTCACC ATGGACGAAG GTCTGTCGCG GGCGGTGCGC ACGATCTTCA AGCGGCTTTA TGACGCCGGG CTGATCTATC GGGCCGAGCG GCTGGTCAAC TGGTCGCCGG TGCTGCAGAC CGCGATCTCC GACCTCGAGG TCAACTACCG CGACGTCGAA GGCGAGCTGG TGTCGTTTAG GTACGGCTCG CTTGACGACT CGCAACCCCA CATCGTGGTC GCCACCACCC GGGTCGAGAC GATGCTGGGC GATACCGCGA TCGCCGTCCA TCCCGATGAC GAGCGCTACC GTCACCTGGT CGGCACCAGC CTGGCGCACC CATTCGTCGA CCGGGAGCTG GCCATTGTCG CCGACGAGCA CGTGGACCCT GAATTCGGCA CCGGCGCGGT CAAAGTCACA CCCGCCCACG ACCCCAACGA CTTCGAAATC GGGGTGCGCC ACCAGCTGCC GATGCCCTCG ATCCTGGACA CCAAGGGCCG GATCGTCGAC ACCGGAACGC GATTCGACGG CATGGACCGC TTCGAGGCAC GGGTCGCGGT GCGCCAAGCG CTCGCGGCCC AGGGCCGCGT GGTCGAAGAA AAGCGACCCT ACCTGCACAG CGTCGGACAC TCCGAACGCA GCGGCGAGCC GATCGAGCCG CGGCTATCCC TGCAGTGGTG GGTCCGGGTG GAATCGCTGG CCAAAGCGGC CGGGGATGCG GTGCGCAACG GGGACACCGT GATTCACCCG GCCAGCATGG AACCCCGCTG GTTCTCCTGG GTCGACGACA TGCACGACTG GTGCATCTCG CGACAGCTCT GGTGGGGGCA TCGGATCCCG ATCTGGTACG GACCCGACGG CGAACAGGTG TGCGTCGGCC CGGACGAAAC ACCCCCGCAG GGCTGGGAAC AGGATCCTGA CGTGCTGGAT ACCTGGTTTT CGTCGGCGCT GTGGCCGTTT TCCACGCTGG GTTGGCCGGA CAAGACGGCG GAGCTGGAAA AGTTCTATCC GACAAGCGTT CTGGTTACCG GCTATGACAT CTTGTTCTTT TGGGTGGCCA GAATGATGAT GTTCGGCACC TTCGTCGGCG ACGACGCCGC CATCACCCTC GACGGCCGCC GGGGCCCGCA GGTGCCGTTC ACCGACGTGT TTCTGCATGG GCTGATCCGC GACGAGTCTG GCCGCAAGAT GAGCAAGTCC AAGGGCAACG TCATCGACCC GCTGGATTGG GTGGAAATGT TCGGGGCCGA TGCGCTGCGG TTCACGCTGG CCCGCGGGGC CAGTCCCGGT GGTGACTTGG CGGTGAGCGA GGATGCCGTG CGGGCGTCGC GCAATTTCGG GACCAAGCTG TTCAACGCCA CTCGGTACGC ACTGCTCAAT GGCGCCGCGC CAGCACCCCT GCCATCGCCG AACGAGCTGA CCGACGCCGA CCGCTGGATT CTCGGAAGGT TGGAAGAGGT TCGGGCCGAA GTTGATTCGG CCTTCGACGG ATACGAGTTC AGCCGCGCTT GTGAGTCCCT GTATCACTTC GCCTGGGACG AATTCTGCGA CTGGTACCTC GAACTGGCCA AAACGCAGCT TGCCCAGGGA CTCACACACA CCACCGCCGT GCTGGCCGCC GGGCTGGACA CGCTGCTGCG CCTGCTGCAC CCGGTGATTC CCTTCCTCAC CGAGGCGCTA TGGCTGGCGC TGACCGGCAG GGAATCGCTG GTCAGCGCCG ACTGGCCGGA GCCTTCCGGG ATTAGCGTGG ACCTTGTTGC CGCGCAACGG ATTAACGATA TGCAGAAGTT GGTGACCGAA GTGCGGCGGT TCCGCAGCGA TCAAGGTCTG GCCGACCGGC AGAAGGTTCC GGCCCGAATG CACGGTGTGC GGGACTCGGA TCTGAGCAAC CAGGTGGCCG CCGTGACCTC GCTGGCGTGG CTCACCGAGC CGGGCCCGGA TTTTGAGCCG TCGGTCTCGT TGGAGGTTCG GCTCGGCCCC GAGATGAACC GCACCGTCGT CGTCGAGCTC GACACCTCGG GCACCATCGA CGTGGCCGCC GAGCGTCGCC GCCTGGAAAA GGAGTTGGCC GGCGCCCAAA AGGAGCTGGC GTCGACCGCC GCCAAGTTGG CCAACGCGGA CTTTCTGGCC AAAGCGCCCG ACGCCGTCAT TGCCAAGATC CGGGACCGCC AGCGCGTGGC GCAGCAGGAA ACCGAGCGCA TCACCACCCG GTTGGCTGCG CTGCAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA