MytuD.18822.a

Putative gluconeogenesis factor

CENTER ID: MytuD.18822.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18822.a.B1.GE37849 full length 1 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1587
RefSeq: NP_215938.1
RefSeq: YP_006514802.1
TubercuList: Rv1422
MTB Network Portal: Rv1422
UniProt: P9WMU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDGIVALGG GHGLYATLSA ARRLTPYVTA VVTVADDGGS SGRLRSELDV VPPGDLRMAL AALASDSPHG RLWATILQHR FGGSGALAGH PIGNLMLAGL SEVLADPVAA LDELGRILGV KGRVLPMCPV ALQIEADVSG LEADPRMFRL IRGQVAIATT PGKVRRVRLL PTDPPATRQA VDAIMAADLV VLGPGSWFTS VIPHVLVPGL AAALRATSAR RALVLNLVAE PGETAGFSVE RHLHVLAQHA PGFTVHDIII DAERVPSERE REQLRRTATM LQAEVHFADV ARPGTPLHDP GKLAAVLDGV CARDVGASEP PVAATQEIPI DGGRPRGDDA WR
NT Sequence
atgaccgatg gcatcgtcgc gctgggcggc ggacacggct tgtatgcgac gctgtctgcg gcccgccggt tgacacccta cgttaccgcc gtggtgaccg tcgccgatga cggtggctcg tcgggccggc tgcgcagcga gctcgatgtg gtgccgccgg gcgatctgcg aatggccttg gcggcgttgg catccgatag cccgcacgga cgcctgtggg caactattct gcagcacaga ttcggcggca gtggtgcgct ggccggacat ccgatcggca atctgatgct agcgggcctg tccgaggtgc tggccgatcc ggtcgcggct cttgacgaac tcgggcgcat cctcggggtg aaaggcaggg tgctgccgat gtgcccggtc gcgcttcaga tcgaggccga tgtctccggt ctggaggccg acccgcgcat gttccgcctg atccgtggcc aggtggcgat cgcgaccacg cccggaaagg tgcgccgggt gcggctgctg ccgactgacc cgccggcgac ccggcaggct gtcgacgcca tcatggctgc cgatctggtg gtcctggggc ccgggtcgtg gttcaccagc gtgatacccc atgtgctggt gccgggtctg gccgcagcgc tgcgagcaac gtcggcccgc cgtgccctgg tgctcaacct ggtggctgaa ccgggagaga cggccggttt ctcggtggag cgtcatctgc acgtgctagc ccaacacgcg cccgggttca ccgttcacga catcatcatc gacgccgaac gagtgccgag cgaacgggag cgggagcaac tgcgccgcac ggcgacgatg ctgcaggccg aggtccactt cgccgatgtc gccagacctg gtacaccttt acatgacccg ggcaagctgg cggcggtcct cgacggggtg tgtgcgcgcg acgtcggcgc gtcggagcct ccggtggcgg ccacacagga gataccgatc gacggtggac gaccgagggg tgacgacgcg tggcgatga
Details for MytuD.18822.a.B1.GE37849
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT DGIVALGGGH GLYATLSAAR RLTPYVTAVV TVADDGGSSG RLRSELDVVP PGDLRMALAA LASDSPHGRL WATILQHRFG GSGALAGHPI GNLMLAGLSE VLADPVAALD ELGRILGVKG RVLPMCPVAL QIEADVSGLE ADPRMFRLIR GQVAIATTPG KVRRVRLLPT DPPATRQAVD AIMAADLVVL GPGSWFTSVI PHVLVPGLAA ALRATSARRA LVLNLVAEPG ETAGFSVERH LHVLAQHAPG FTVHDIIIDA ERVPSERERE QLRRTATMLQ AEVHFADVAR PGTPLHDPGK LAAVLDGVCA RDVGASEPPV AATQEIPIDG GRPRGDDAWR
Validated NT Sequence
tactcactta tcgccacgcg tcgtcacccc tcggtcgtcc accgtcgatc ggtatctcct gtgtggccgc caccggaggc tccgacgcgc cgacgtcgcg cgcacacacc ccgtcgagga ccgccgccag cttgcccggg tcatgtaaag gtgtaccagg tctggcgaca tcggcgaagt ggacctcggc ctgcagcatc gtcgccgtgc ggcgcagttg ctcccgctcc cgttcgctcg gcactcgttc ggcgtcgatg atgatgtcgt gaacggtgaa cccgggcgcg tgttgggcta gcacgtgcag atgacgctcc accgagaaac cggccgtctc tcccggttca gccaccaggt tgagcaccag ggcacggcgg gccgacgttg ctcgcagcgc tgcggccaga cccggcacca gcacatgggg tatcacgctg gtgaaccacg acccgggccc caggaccacc agatcggcag ccatgatggc gtcgacagcc tgccgggtcg ccggcgggtc agtcggcagc agccgcaccc ggcgcacctt tccgggcgtg gtcgcgatcg ccacctggcc acggatcagg cggaacatgc gcgggtcggc ctccagaccg gagacatcgg cctcgatctg aagcgcgacc gggcacatcg gcagcaccct gcctttcacc ccgaggatgc gcccgagttc gtcaagagcc gcgaccggat cggccagcac ctcggacagg cccgctagca tcagattgcc gatcggatgt ccggccagcg caccactgcc gccgaatctg tgctgcagaa tagttgccca caggcgtccg tgcgggctat cggatgccaa cgccgccaag gccattcgca gatcgcccgg cggcaccaca tcgagctcgc tgcgcagccg gcccgacgag ccaccgtcat cggcgacggt caccacggcg gtaacgtagg gtgtcaaccg gcgggccgca gacagcgtcg catacaagcc gtgtccgccg cccagcgcga cgatgccatc ggtcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMT DGIVALGGGH GLYATLSAAR RLTPYVTAVV TVADDGGSSG RLRSELDVVP PGDLRMALAA LASDSPHGRL WATILQHRFG GSGALAGHPI GNLMLAGLSE VLADPVAALD ELGRILGVKG RVLPMCPVAL QIEADVSGLE ADPRMFRLIR GQVAIATTPG KVRRVRLLPT DPPATRQAVD AIMAADLVVL GPGSWFTSVI PHVLVPGLAA ALRATSARRA LVLNLVAEPG ETAGFSVERH LHVLAQHAPG FTVHDIIIDA ERVPSERERE QLRRTATMLQ AEVHFADVAR PGTPLHDPGK LAAVLDGVCA RDVGASEPPV AATQEIPIDG GRPRGDDAWR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg atggcatcgt cgcgctgggc ggcggacacg gcttgtatgc gacgctgtct gcggcccgcc ggttgacacc ctacgttacc gccgtggtga ccgtcgccga tgacggtggc tcgtcgggcc ggctgcgcag cgagctcgat gtggtgccgc cgggcgatct gcgaatggcc ttggcggcgt tggcatccga tagcccgcac ggacgcctgt gggcaactat tctgcagcac agattcggcg gcagtggtgc gctggccgga catccgatcg gcaatctgat gctagcgggc ctgtccgagg tgctggccga tccggtcgcg gctcttgacg aactcgggcg catcctcggg gtgaaaggca gggtgctgcc gatgtgcccg gtcgcgcttc agatcgaggc cgatgtctcc ggtctggagg ccgacccgcg catgttccgc ctgatccgtg gccaggtggc gatcgcgacc acgcccggaa aggtgcgccg ggtgcggctg ctgccgactg acccgccggc gacccggcag gctgtcgacg ccatcatggc tgccgatctg gtggtcctgg ggcccgggtc gtggttcacc agcgtgatac cccatgtgct ggtgccgggt ctggccgcag cgctgcgagc aacgtcggcc cgccgtgccc tggtgctcaa cctggtggct gaaccgggag agacggccgg tttctcggtg gagcgtcatc tgcacgtgct agcccaacac gcgcccgggt tcaccgttca cgacatcatc atcgacgccg aacgagtgcc gagcgaacgg gagcgggagc aactgcgccg cacggcgacg atgctgcagg ccgaggtcca cttcgccgat gtcgccagac ctggtacacc tttacatgac ccgggcaagc tggcggcggt cctcgacggg gtgtgtgcgc gcgacgtcgg cgcgtcggag cctccggtgg cggccacaca ggagataccg atcgacggtg gacgaccgag gggtgacgac gcgtggcgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat