SSGCID: Target Page

MytuD.18819.a

Conserved protein

CENTER ID:	MytuD.18819.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18819.a.B1.GE37846	full length		1	470

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.4144
RefSeq:	NP_218236.1
RefSeq:	YP_006517211.1
TubercuList:	Rv3719
MTB Network Portal:	Rv3719
UniProt:	O69686

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQGQLSRTRV YTVPVPGSAQ SAYACGVERL LASYRSIPAT ASIRLAKPTS NLFRARVKHD ARGLDASGLT GVIGIDPEAR TADVAGMCTY EDLIAATLHY GLSPLVVPQL RTITLGGAVT GLGIESASFR NGLPHESVLE MDILTGAGEL LTVSPGQHSD LYRAFPNSYG TLGYSTRLRI QLEPVRPFVA LRHIRFSSLT AMVAAMERII DTGGLDGESV DYLDGVVFSA DESYLCIGMQ TSVPGPVSDY TGQDIYYRSI QHEAGIKEDR LTIHDYFWRW DTDWFWCSRS FGAQNPRLRR WWPRRYRRSS VYWRLMALDQ RFGIADRFEN SRGRPARERV VQDIEVPIER TCEFLEWFGE NVPISPIWLC PLRLRDHAGW PLYPIRPDRS YVNIGFWSSV PVGATEGATN RKIENKVSAL DGHKSLYSDS FYTREEFDEL YGGETYNTVK KAYDPDSRLL DLYAKAVQRR

NT Sequence

atgcaggggc agttgtcgag gactagggta tatacggtgc ctgtccctgg atctgcacag tcggcttacg cctgcggcgt cgagcggttg ctggcgagct atcgatccat ccccgcgact gcatccatcc ggcttgccaa gcccacctca aatctgttcc gcgcccgcgt caaacacgat gcacgcggcc tggacgcatc gggactgacc ggtgtcatcg gtatcgatcc cgaggcccgc accgccgacg tggccggcat gtgcacatac gaggacctaa tcgccgcgac actgcactac ggtctgtcac cattggtggt tccgcagctg aggacgatca cattgggcgg agcggtcacc ggcttgggta tcgagtcggc gtcgttccgc aacggcctgc cccacgagtc ggtgctggag atggatatcc tcaccggcgc aggagaactt ctcaccgtct cgcccggaca gcactccgac ttgtaccgtg cattccctaa ctcgtatggg acactgggct attcaacccg gcttcgaatc cagctggagc cggtccggcc gtttgtcgcg ctgcggcaca tccgatttag ctcgttgacg gcgatggtgg ccgcaatgga gcgcatcatc gacaccggcg gactggacgg cgaatcggtg gactatctcg acggggtggt tttcagcgct gacgaaagct acctgtgcat cggcatgcag acgagcgtac cgggcccggt cagcgactac accggacaag acatctacta ccggtcgatc caacacgagg cggggatcaa ggaagaccgg ttgaccatcc acgattactt ctggcgctgg gacaccgatt ggttctggtg ctcacgatcg tttggtgccc aaaacccgcg gctgcgccgc tggtggccgc ggcgctaccg gcgtagcagt gtctactgga ggttgatggc gctcgatcag cgcttcggga tcgccgaccg gttcgagaac agcaggggtc gtcccgcgcg tgaacgggtg gtgcaggata tcgaagtgcc gatcgaacgg acctgcgagt ttctggagtg gttcggggaa aacgtgccca tttcgccaat ctggttgtgc ccgttgcggc tacgcgatca cgccggctgg ccgctgtacc cgatccggcc tgaccgtagc tatgtcaaca tcgggttctg gtcgtcggtg ccggttggcg ccaccgaggg cgccaccaac cgcaagatcg agaacaaggt gagtgcgctc gacgggcaca agtcgctcta ctccgactcc ttctataccc gcgaggagtt cgacgagctc tacggcggcg agacttacaa cactgtgaag aaagcctacg atcccgattc gcgtctcctc gatctttacg caaaggcggt gcaacgacga tga

Details for MytuD.18819.a.B1.GE37846

HARVESTED ON:	3/6/2014
SEQUENCED ON:	3/13/2014
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	66

Validated AA Sequence

Validated NT Sequence

cnctagaata ttttgtttnn tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagg ggcagttgtc gaggactagg gtatatacgg tgcctgtccc tggatctgca cagtcggctt acgcctgcgg cgtcgagcgg ttgctggcga gctatcgatc catccccgcg actgcatcca tccggcttgc caagcccacc tcaaatctgt tccgcgcccg cgtcaaacac gatgcacgcg gcctggacgc atcgggactg accggtgtca tcggtatcga tcccgaggcc cgcaccgccg acgtggccgg catgtgcaca tacgaggacc taatcgccgc gacactgcac tacggtctgt caccattggt ggttccgcag ctgaggacga tcacattggg cggagcggtc accggcttgg gtatcgagtc ggcgtcgttc cgcaacggcc tgccccacga gtcggtgctg gagatggata tcctcaccgg cgcaggagaa cttctcaccg tctcgcccgg acagcactcc gacttgtacc gtgcattccc taactcgtat gggacactgg gctattcaac ccggcttcga atccagctgg agccggtccg gccgtttgtc gcgctgcggc acatccgatt tagctcgttg acggcgatgg tggccgcaat ggagcgcatc atcgacaccg gcggactgga cggcgaatcg gtggactatc tcgacggggt ggttttcagc gctgacgaaa gctacctgtg catcggcatg cagacgagcg taccgggccc ggtcagcgac tacaccggac aagacatcta ctaccggtcg atccaacacg aggcggggat caaggaagac cggttgacca tccacgatta cttctggcgc tgggacaccg attggttctg gtgctcacga tcgtttggtg cccaaaaccc gcggctgcgc cgctggtggc cgcggcgcta ccgggcgtag cagtgtctac tggnngttga tggcgctcga tcagcgcttc gggatcgccg accggttcga ganagcaggg gtcgtcccgc gcgtgacggn ngngctatcn antgcnatcg acggactgnn ntttctggan nnnngggaaa cnnncccatt tcncancnnn nnnccntgcg ntanncgaat cnncnnnnnn nctnccgaat cgncngacnt annnnnntca nnnnngnnnn nnnnnnctnn ggnnnnnngg ncnncacncn ngaggggnnn ctnnncta

Expected Protein Sequence

MAHHHHHHMQ GQLSRTRVYT VPVPGSAQSA YACGVERLLA SYRSIPATAS IRLAKPTSNL FRARVKHDAR GLDASGLTGV IGIDPEARTA DVAGMCTYED LIAATLHYGL SPLVVPQLRT ITLGGAVTGL GIESASFRNG LPHESVLEMD ILTGAGELLT VSPGQHSDLY RAFPNSYGTL GYSTRLRIQL EPVRPFVALR HIRFSSLTAM VAAMERIIDT GGLDGESVDY LDGVVFSADE SYLCIGMQTS VPGPVSDYTG QDIYYRSIQH EAGIKEDRLT IHDYFWRWDT DWFWCSRSFG AQNPRLRRWW PRRYRRSSVY WRLMALDQRF GIADRFENSR GRPARERVVQ DIEVPIERTC EFLEWFGENV PISPIWLCPL RLRDHAGWPL YPIRPDRSYV NIGFWSSVPV GATEGATNRK IENKVSALDG HKSLYSDSFY TREEFDELYG GETYNTVKKA YDPDSRLLDL YAKAVQRR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagg ggcagttgtc gaggactagg gtatatacgg tgcctgtccc tggatctgca cagtcggctt acgcctgcgg cgtcgagcgg ttgctggcga gctatcgatc catccccgcg actgcatcca tccggcttgc caagcccacc tcaaatctgt tccgcgcccg cgtcaaacac gatgcacgcg gcctggacgc atcgggactg accggtgtca tcggtatcga tcccgaggcc cgcaccgccg acgtggccgg catgtgcaca tacgaggacc taatcgccgc gacactgcac tacggtctgt caccattggt ggttccgcag ctgaggacga tcacattggg cggagcggtc accggcttgg gtatcgagtc ggcgtcgttc cgcaacggcc tgccccacga gtcggtgctg gagatggata tcctcaccgg cgcaggagaa cttctcaccg tctcgcccgg acagcactcc gacttgtacc gtgcattccc taactcgtat gggacactgg gctattcaac ccggcttcga atccagctgg agccggtccg gccgtttgtc gcgctgcggc acatccgatt tagctcgttg acggcgatgg tggccgcaat ggagcgcatc atcgacaccg gcggactgga cggcgaatcg gtggactatc tcgacggggt ggttttcagc gctgacgaaa gctacctgtg catcggcatg cagacgagcg taccgggccc ggtcagcgac tacaccggac aagacatcta ctaccggtcg atccaacacg aggcggggat caaggaagac cggttgacca tccacgatta cttctggcgc tgggacaccg attggttctg gtgctcacga tcgtttggtg cccaaaaccc gcggctgcgc cgctggtggc cgcggcgcta ccggcgtagc agtgtctact ggaggttgat ggcgctcgat cagcgcttcg ggatcgccga ccggttcgag aacagcaggg gtcgtcccgc gcgtgaacgg gtggtgcagg atatcgaagt gccgatcgaa cggacctgcg agtttctgga gtggttcggg gaaaacgtgc ccatttcgcc aatctggttg tgcccgttgc ggctacgcga tcacgccggc tggccgctgt acccgatccg gcctgaccgt agctatgtca acatcgggtt ctggtcgtcg gtgccggttg gcgccaccga gggcgccacc aaccgcaaga tcgagaacaa ggtgagtgcg ctcgacgggc acaagtcgct ctactccgac tccttctata cccgcgagga gttcgacgag ctctacggcg gcgagactta caacactgtg aagaaagcct acgatcccga ttcgcgtctc ctcgatcttt acgcaaaggc ggtgcaacga cgatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat