MytuD.18818.a

Uncharacterized protein

CENTER ID: MytuD.18818.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18818.a.B1.GE37845 full length 1 939
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1325
RefSeq: NP_215695.1
RefSeq: YP_006514555.1
TubercuList: Rv1179c
MTB Network Portal: Rv1179c
UniProt: O50435

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDPHRDLESR AFAGNWRVYQ QQALDAFDAD VAAGDNRAYL VLPPGAGKTM IGLEAARRLG RRSLVLVPNT AVQAQWAAAW DNSFPSSDRS ASKCGTERGL ASAMNVLTYQ SLAVIDAETD STVRREVLRN RDQQALLDLL HPNGRAVIER AATLGPWTLV LDECHHLLAT WGALVSALAS VLGAQTALIG LTATPATELT AWQHTLHDEL FGTADFVIPT PALVREGDLA PYQELVYLTQ PTPEEQAWIG THRARFADLM LALIDQKVGS MSLAAWLHTR IVDRATREGN QIAWSTFERA EPDLACSGLR FAYDGLIPLP DGVRLREQHR IAPDAQDWVN VLTDFSVGHL QQSADPRDAH ALTAIKRVLP GLGYRLTSRG VRVATSPVDR LCALSESKIA ATAHILDTED AVLGARLRAL VLCDFESMTG ALPTSLKGAP VSEQSGSAQL VAAMLAASDH RRRTPLHALL VTGQTFACPA AIEDDLIAFC AERGALVTAE PLDAHPSLRV MRGTGGFTPR TWVALATEYF LAGRARVLVG TRSLLGEGWD CAAVNVNIDL TSATTQAAIT QMRGRAIRND PSDGHKVADN WSVCCIATEH PRGDADYLRL VRKHDGYYAA TPQGLIESGV THCDPSLSPY GPPVTDTHAI TARALQRVAE RAQARSWWRI GEPYEGVDVA TIRVRSRQPL GVAAPRIPAS ALTPPVPGQF SPVRLARGAV AAVSVVGAST ATAVASANLG MLAGAGTAGA IVAAGVGLVA TAAAAESRRL DHAPNALEQL AAVVADALYA AGGAQRGSAA LRLASDPEGW IRCQLDGVPT EQSLRFTAAL DELLAPLAEP RYLIGRKILT PPARPVARRL FAVRAVVGLS LPGTVAWHAV PRWFARNKDR RQHLAQAWRK HIGPPRQLPA DSPQGQAILD LFRGDNPLSV TTQLRTTWR
NT Sequence
gtggatcctc accgcgacct ggaatcgcgg gcctttgccg gcaactggcg ggtataccaa cagcaagcgt tggacgcgtt cgacgccgac gtcgctgccg gcgacaaccg tgcctatctg gtgctgccgc cgggtgcggg caagacgatg atcggcctgg aagcagcgcg ccggctgggg cgccggagtt tggtgttggt tcccaacacg gcggtgcagg cgcagtgggc cgccgcgtgg gataacagtt ttccgtcgtc ggaccggtcg gcatcgaagt gtggaaccga gcgtggcctt gcctcggcga tgaacgtcct gacgtatcag tcgcttgccg tcatcgacgc cgaaaccgat tcgacagtcc ggcgggaagt cctgcgcaac cgcgaccagc aagcgttgct ggatctcctg caccccaacg ggagggcggt gatcgagcgg gcggcgacgc taggcccgtg gacgctggtg ctcgatgagt gccaccatct gctagctacg tggggcgccc tggtcagtgc gttggcgtcg gtcctcggag cgcagaccgc gctgatcggt ctaacggcga ccccggccac agagctcacc gcgtggcagc acaccctgca tgatgagctg ttcggcaccg ccgacttcgt gatcccgaca cccgctctgg ttagggaagg cgacctggct ccctaccaag agttggtcta tctgacccaa ccgacgcccg aagagcaggc ctggatcggc acccaccggg cgcgcttcgc cgacctcatg ctggccctca tcgaccaaaa ggtgggcagc atgtcgttgg ccgcgtggct gcacacccgg atcgtggatc gagcgacgcg cgagggcaat cagatcgcct ggtcgacgtt cgagcgtgcc gaacccgacc tcgcgtgcag cggcctgcgc ttcgcctacg acggcctgat tccactaccc gacggcgtgc gcctgcgcga gcagcaccga attgcgcccg atgcccagga ctgggttaac gtattgaccg acttcagcgt cgggcacctg caacaaagcg cggatccgcg cgacgcgcac gcgctgaccg cgatcaagcg ggtgctaccc ggcctgggct accggctgac cagtcgcggc gtacgcgtcg cgacctcgcc agtagacaga ctgtgcgcgc tgtccgaatc caagatcgcc gcgaccgcgc acatcctcga caccgaggac gccgtcttgg gggcgcggtt gcgggcgctg gtgctctgcg atttcgaatc gatgaccggc gcccttccca catcgctgaa gggcgcaccc gtcagcgagc agtcgggttc ggcccagctg gttgccgcca tgctcgccgc gtcggatcac cgccgccgca ccccactgca cgcccttctg gtaacaggtc aaaccttcgc ctgcccggcc gcgatcgaag atgacctgat cgccttctgc gccgagcgcg gcgcgctcgt caccgccgag ccgcttgacg cccaccctag tctgcgggtc atgcgcggca ccggcggctt cacgccacgt acgtgggtcg cgctggctac cgaatacttc ctggccggcc gcgcccgcgt cctggtcggc acccgttcgc tactaggtga agggtgggac tgcgcggcgg tcaacgtcaa tatcgacctg acgagcgcaa ccacccaggc agcgatcact cagatgcgcg gccgcgccat ccgcaacgac ccctcggacg gtcacaaggt ggcggacaac tggtcggtct gctgtatcgc cacagaacac ccgcgcggtg atgccgacta cctgcgcctg gtgcgcaaac atgacgggta ctacgcggca accccgcagg ggctcatcga atcgggtgtg acgcactgcg atccgtcgct gtcgccgtac ggtcctccgg tcaccgatac ccacgccatc acggcgcgag cgctgcagcg cgtcgccgaa cgcgcccagg cgagatcctg gtggcgaatc ggcgagccct acgaaggagt cgacgtcgca accatccgcg tgcgctcccg ccagccgctc ggggtcgccg caccccgcat ccccgcctcg gcattgaccc caccggtgcc gggacagttc agtccggtcc gcctggcaag gggtgccgtg gccgccgttt ccgtggtcgg cgccagcacc gccaccgcag ttgcctccgc caatctcggc atgctggcgg gtgccggcac ggccggcgcc atcgtcgcgg cgggtgttgg cctggtggca accgctgcgg cggccgaaag ccgccgcctg gaccacgcgc ccaacgcgct cgagcaactg gccgccgtcg tcgcggatgc gctgtacgcc gcgggcggcg cacagcgagg atccgcggca ctacggttgg cctccgaccc cgaaggctgg atccggtgtc aactcgacgg cgtgcccacc gagcagtccc tgcggttcac cgcggccctc gacgagctac tggcgccgct tgccgaaccg cgctacctca tcggacgcaa gatcctcacg ccgcccgccc ggcccgttgc ccggaggctg ttcgcggttc gtgccgtcgt cgggctatcg ctgcccggca cggttgcctg gcacgccgtg ccccgctggt tcgcacgaaa caaggatcgg cggcaacatc tggcacaggc atggcgaaag cacatcggcc caccccgaca actgccagca gactccccac aagggcaagc catcctcgat ctgttccgcg gcgataaccc gctctcggtg acaacccagc tgcgcacgac ctggcggtga
Details for MytuD.18818.a.B1.GE37845
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 102kDa
OBSERVED MW: 102kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 34
Validated AA Sequence
MAHHHHHHMV DPHRDLESRA FAGNWRVYQQ QALDAFDADV AAGDNRAYLV LPPGAGKTMI GLEAARRLGR RSLVLVPNTA VQAQWAAAWD NSFPSSDRSA SKCGTERGLA SAMNVLTYQS LAVIDAETDS TVRREVLRNR DQQALLDLLH PNGRAVIERA ATLGPWTLVL DECHHLLATW GALVSALASV LGAQTALIGL TATPATELTA WQHTLHDELF GTADFVIPTP ALVREGDLAP YQELVYLTQP TPEEQAWIGT HRARFADLML ALIDQKVGSM SLAAWLHTRI VDRATREGNQ IAWSTFERAE PDLACSGLRF ATTA
Validated NT Sequence
cnctagaaat attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatggt ggatcctcac cgcgacctgg aatcgcgggc ctttgccggc aactggcggg tataccaaca gcaagcgttg gacgcgttcg acgccgacgt cgctgccggc gacaaccgtg cctatctggt gctgccgccg ggtgcgggca agacgatgat cggcctggaa gcagcgcgcc ggctggggcg ccggagtttg gtgttggttc ccaacacggc ggtgcaggcg cagtgggccg ccgcgtggga taacagtttt ccgtcgtcgg accggtcggc atcgaagtgt ggaaccgagc gtggccttgc ctcggcgatg aacgtcctga cgtatcagtc gcttgccgtc atcgacgccg aaaccgattc gacagtccgg cgggaagtcc tgcgcaaccg cgaccagcaa gcgttgctgg atctcctgca ccccaacggg agggcggtga tcgagcgggc ggcgacgcta ggcccgtgga cgctggtgct cgatgagtgc caccatctgc tagctacgtg gggcgccctg gtcagtgcgt tggcgtcggt cctcggagcg cagaccgcgc tgatcggtct aacggcgacc ccggccacag agctcaccgc gtggcagcac accctgcatg atgagctgtt cggcaccgcc gacttcgtga tcccgacacc cgctctggtt agggaaggcg acctggctcc ctaccaagag ttggtctatc tgacccaacc gacgcccgaa gagcaggcct ggatcggcac ccaccgggcg cgcttcgccg acctcatgct ggccctcatc gaccaaaagg tgggcagcat gtcgttggcc gcgtggctgc acacccggat cgtggatcga gcgacgcgcg agggcaatca gatcgcctgg tcgacgttcg agcgtgccga acccgacctc gcgtgcagcg gcctgcgctt cgctacgacg gcctgattcc actacccgac ggcgtgcgcc tgcgcgagca gcaccgaatt gcgcccgatg cccaggactg gnnnngtatt gacgacttca gcgtnnncac ctgcanaaag cnnngatccn nnngacnccn nnnnnnnctg acnnnatcag cngggtgcta ncnnnnggnn ncnnntgaca ntcncggnnn acgcnnnngg accnnccnnn nnnnanannn nnnnnncnnn nnantcnnna nnnnnnngna ccnnnnnnna atcnctt
Expected Protein Sequence
MAHHHHHHMD PHRDLESRAF AGNWRVYQQQ ALDAFDADVA AGDNRAYLVL PPGAGKTMIG LEAARRLGRR SLVLVPNTAV QAQWAAAWDN SFPSSDRSAS KCGTERGLAS AMNVLTYQSL AVIDAETDST VRREVLRNRD QQALLDLLHP NGRAVIERAA TLGPWTLVLD ECHHLLATWG ALVSALASVL GAQTALIGLT ATPATELTAW QHTLHDELFG TADFVIPTPA LVREGDLAPY QELVYLTQPT PEEQAWIGTH RARFADLMLA LIDQKVGSMS LAAWLHTRIV DRATREGNQI AWSTFERAEP DLACSGLRFA YDGLIPLPDG VRLREQHRIA PDAQDWVNVL TDFSVGHLQQ SADPRDAHAL TAIKRVLPGL GYRLTSRGVR VATSPVDRLC ALSESKIAAT AHILDTEDAV LGARLRALVL CDFESMTGAL PTSLKGAPVS EQSGSAQLVA AMLAASDHRR RTPLHALLVT GQTFACPAAI EDDLIAFCAE RGALVTAEPL DAHPSLRVMR GTGGFTPRTW VALATEYFLA GRARVLVGTR SLLGEGWDCA AVNVNIDLTS ATTQAAITQM RGRAIRNDPS DGHKVADNWS VCCIATEHPR GDADYLRLVR KHDGYYAATP QGLIESGVTH CDPSLSPYGP PVTDTHAITA RALQRVAERA QARSWWRIGE PYEGVDVATI RVRSRQPLGV AAPRIPASAL TPPVPGQFSP VRLARGAVAA VSVVGASTAT AVASANLGML AGAGTAGAIV AAGVGLVATA AAAESRRLDH APNALEQLAA VVADALYAAG GAQRGSAALR LASDPEGWIR CQLDGVPTEQ SLRFTAALDE LLAPLAEPRY LIGRKILTPP ARPVARRLFA VRAVVGLSLP GTVAWHAVPR WFARNKDRRQ HLAQAWRKHI GPPRQLPADS PQGQAILDLF RGDNPLSVTT QLRTTWR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggatc ctcaccgcga cctggaatcg cgggcctttg ccggcaactg gcgggtatac caacagcaag cgttggacgc gttcgacgcc gacgtcgctg ccggcgacaa ccgtgcctat ctggtgctgc cgccgggtgc gggcaagacg atgatcggcc tggaagcagc gcgccggctg gggcgccgga gtttggtgtt ggttcccaac acggcggtgc aggcgcagtg ggccgccgcg tgggataaca gttttccgtc gtcggaccgg tcggcatcga agtgtggaac cgagcgtggc cttgcctcgg cgatgaacgt cctgacgtat cagtcgcttg ccgtcatcga cgccgaaacc gattcgacag tccggcggga agtcctgcgc aaccgcgacc agcaagcgtt gctggatctc ctgcacccca acgggagggc ggtgatcgag cgggcggcga cgctaggccc gtggacgctg gtgctcgatg agtgccacca tctgctagct acgtggggcg ccctggtcag tgcgttggcg tcggtcctcg gagcgcagac cgcgctgatc ggtctaacgg cgaccccggc cacagagctc accgcgtggc agcacaccct gcatgatgag ctgttcggca ccgccgactt cgtgatcccg acacccgctc tggttaggga aggcgacctg gctccctacc aagagttggt ctatctgacc caaccgacgc ccgaagagca ggcctggatc ggcacccacc gggcgcgctt cgccgacctc atgctggccc tcatcgacca aaaggtgggc agcatgtcgt tggccgcgtg gctgcacacc cggatcgtgg atcgagcgac gcgcgagggc aatcagatcg cctggtcgac gttcgagcgt gccgaacccg acctcgcgtg cagcggcctg cgcttcgcct acgacggcct gattccacta cccgacggcg tgcgcctgcg cgagcagcac cgaattgcgc ccgatgccca ggactgggtt aacgtattga ccgacttcag cgtcgggcac ctgcaacaaa gcgcggatcc gcgcgacgcg cacgcgctga ccgcgatcaa gcgggtgcta cccggcctgg gctaccggct gaccagtcgc ggcgtacgcg tcgcgacctc gccagtagac agactgtgcg cgctgtccga atccaagatc gccgcgaccg cgcacatcct cgacaccgag gacgccgtct tgggggcgcg gttgcgggcg ctggtgctct gcgatttcga atcgatgacc ggcgcccttc ccacatcgct gaagggcgca cccgtcagcg agcagtcggg ttcggcccag ctggttgccg ccatgctcgc cgcgtcggat caccgccgcc gcaccccact gcacgccctt ctggtaacag gtcaaacctt cgcctgcccg gccgcgatcg aagatgacct gatcgccttc tgcgccgagc gcggcgcgct cgtcaccgcc gagccgcttg acgcccaccc tagtctgcgg gtcatgcgcg gcaccggcgg cttcacgcca cgtacgtggg tcgcgctggc taccgaatac ttcctggccg gccgcgcccg cgtcctggtc ggcacccgtt cgctactagg tgaagggtgg gactgcgcgg cggtcaacgt caatatcgac ctgacgagcg caaccaccca ggcagcgatc actcagatgc gcggccgcgc catccgcaac gacccctcgg acggtcacaa ggtggcggac aactggtcgg tctgctgtat cgccacagaa cacccgcgcg gtgatgccga ctacctgcgc ctggtgcgca aacatgacgg gtactacgcg gcaaccccgc aggggctcat cgaatcgggt gtgacgcact gcgatccgtc gctgtcgccg tacggtcctc cggtcaccga tacccacgcc atcacggcgc gagcgctgca gcgcgtcgcc gaacgcgccc aggcgagatc ctggtggcga atcggcgagc cctacgaagg agtcgacgtc gcaaccatcc gcgtgcgctc ccgccagccg ctcggggtcg ccgcaccccg catccccgcc tcggcattga ccccaccggt gccgggacag ttcagtccgg tccgcctggc aaggggtgcc gtggccgccg tttccgtggt cggcgccagc accgccaccg cagttgcctc cgccaatctc ggcatgctgg cgggtgccgg cacggccggc gccatcgtcg cggcgggtgt tggcctggtg gcaaccgctg cggcggccga aagccgccgc ctggaccacg cgcccaacgc gctcgagcaa ctggccgccg tcgtcgcgga tgcgctgtac gccgcgggcg gcgcacagcg aggatccgcg gcactacggt tggcctccga ccccgaaggc tggatccggt gtcaactcga cggcgtgccc accgagcagt ccctgcggtt caccgcggcc ctcgacgagc tactggcgcc gcttgccgaa ccgcgctacc tcatcggacg caagatcctc acgccgcccg cccggcccgt tgcccggagg ctgttcgcgg ttcgtgccgt cgtcgggcta tcgctgcccg gcacggttgc ctggcacgcc gtgccccgct ggttcgcacg aaacaaggat cggcggcaac atctggcaca ggcatggcga aagcacatcg gcccaccccg acaactgcca gcagactccc cacaagggca agccatcctc gatctgttcc gcggcgataa cccgctctcg gtgacaaccc agctgcgcac gacctggcgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat