MytuD.18807.a

Uncharacterized protein

CENTER ID: MytuD.18807.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18807.a.B2.GE37840 mature protein 20 249
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.208
RefSeq: NP_214698.1
RefSeq: YP_006513504.1
TubercuList: Rv0184
MTB Network Portal: Rv0184
UniProt: O07428

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNDKMLARI AALLRQAEGT DNPHEADAFM STAQRLATAA SIDLAVARSH AGNRSPAQAP TQRTITIGAA GTRGLRTYVQ LFVLIAAAND VRCDVASNST FVYAYGFAED IDTSHALYAS LVVQMVRASD AYLASGAHRP TPTITARLNF QLAFGARVGQ RLADAREQTR QEATKDRDRP PGTAIALRDK DIELHEYYRR SSKARGAWRA SRATAGYSSA ARRAGDRAGR QARLGNNPEL PGARAALGR
NT Sequence
atgaccaacg acaagatgct ggcccgcatc gcagccctgc tgcgccaggc cgaaggcacc gacaacccgc acgaggccga cgcgttcatg agcaccgcac aacggttggc cacggcggca tccatcgacc tggcggtggc ccggtcgcac gcgggcaacc gttcacccgc gcaggccccg acacagcgca ccatcaccat cggggcggcg ggcacccgcg gattgcggac ctatgtgcag ctcttcgtgc tcatcgcggc ggccaacgac gtgcgctgcg acgtggcatc gaattcgacg ttcgtgtacg cctacgggtt cgccgaggac atcgacacca gccacgccct atacgccagc ctggtggtcc agatggtccg ggcatccgac gcctacctcg cctcgggagc gcaccggccc acgccgacga tcaccgcccg actcaacttc cagctggcgt tcggcgcccg ggtcggccag cgcttggccg atgcccgaga gcagactcgg caggaagcca ccaaggaccg tgatcgtccg cctggtaccg caattgccct gcgggacaag gacatcgagc tgcatgagta ctaccgtcgt tcctctaagg cgcgcggcgc ctggcgagcc agccgggcca ccgcgggata ctcgtcggcg gcacggcgcg ccggtgatcg agcgggacgg caagcacgac tcgggaacaa ccccgagctg cccggggcac gggccgcgct gggccggtga
Details for MytuD.18807.a.B2.GE37840
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT DNPHEADAFM STAQRLATAA SIDLAVARSH AGNRSPAQAP TQRTITIGAA GTRGLRTYVQ LFVLIAAAND VRCDVASNST FVYAYGFAED IDTSHALYAS LVVQMVRASD AYLASGAHRP TPTITARLNF QLAFGARVGQ RLADAREQTR QEATKDRDRP PGTAIALRDK DIELHEYYRR SSKARGAWRA SRATAGYSSA ARRAGDRAGR QARLGNNPEL PGARAALGR
Validated NT Sequence
atcttactca cttaccggcc cagcgcggcc cgtgccccgg gcagctcggg gttgttcccg agtcgtgctt gccgtcccgc tcgatcaccg gcgcgccgtg ccgccgacga gtatcccgcg gtggcccggc tggctcgcca ggcgccgcgc gccttagagg aacgacggta gtactcatgc agctcgatgt ccttgtcccg cagggcaatt gcggtaccag gcggacgatc acggtccttg gtggcttcct gccgagtctg ctctcgggca tcggccaagc gctggccgac ccgggcgccg aacgccagct ggaagttgag tcgggcggtg atcgtcggcg tgggccggtg cgctcccgag gcgaggtagg cgtcggatgc ccggaccatc tggaccacca ggctggcgta tagggcgtgg ctggtgtcga tgtcctcggc gaacccgtag gcgtacacga acgtcgaatt cgatgccacg tcgcagcgca cgtcgttggc cgccgcgatg agcacgaaga gctgcacata ggtccgcaat ccgcgggtgc ccgccgcccc gatggtgatg gtgcgctgtg tcggggcctg cgcgggtgaa cggttgcccg cgtgcgaccg ggccaccgcc aggtcgatgg atgccgccgt ggccaaccgt tgtgcggtgc tcatgaacgc gtcggcctcg tgcgggttgt cggtcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHTD NPHEADAFMS TAQRLATAAS IDLAVARSHA GNRSPAQAPT QRTITIGAAG TRGLRTYVQL FVLIAAANDV RCDVASNSTF VYAYGFAEDI DTSHALYASL VVQMVRASDA YLASGAHRPT PTITARLNFQ LAFGARVGQR LADAREQTRQ EATKDRDRPP GTAIALRDKD IELHEYYRRS SKARGAWRAS RATAGYSSAA RRAGDRAGRQ ARLGNNPELP GARAALGR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataccgaca acccgcacga ggccgacgcg ttcatgagca ccgcacaacg gttggccacg gcggcatcca tcgacctggc ggtggcccgg tcgcacgcgg gcaaccgttc acccgcgcag gccccgacac agcgcaccat caccatcggg gcggcgggca cccgcggatt gcggacctat gtgcagctct tcgtgctcat cgcggcggcc aacgacgtgc gctgcgacgt ggcatcgaat tcgacgttcg tgtacgccta cgggttcgcc gaggacatcg acaccagcca cgccctatac gccagcctgg tggtccagat ggtccgggca tccgacgcct acctcgcctc gggagcgcac cggcccacgc cgacgatcac cgcccgactc aacttccagc tggcgttcgg cgcccgggtc ggccagcgct tggccgatgc ccgagagcag actcggcagg aagccaccaa ggaccgtgat cgtccgcctg gtaccgcaat tgccctgcgg gacaaggaca tcgagctgca tgagtactac cgtcgttcct ctaaggcgcg cggcgcctgg cgagccagcc gggccaccgc gggatactcg tcggcggcac ggcgcgccgg tgatcgagcg ggacggcaag cacgactcgg gaacaacccc gagctgcccg gggcacgggc cgcgctgggc cggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat