MytuD.18805.a

CRISPR-associated endoribonuclease Cas6 (EC 3.1.-.-)

CENTER ID: MytuD.18805.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18805.a.B1.GE37839 full length 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3149
RefSeq: NP_217340.1
RefSeq: YP_006516273.1
TubercuList: Rv2824c
MTB Network Portal: Rv2824c
UniProt: P9WPJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAARRGGIRR TDLLRRSGQP RGRHRASAAE SGLTWISPTL ILVGFSHRGD RRMTEHLSRL TLTLEVDAPL ERARVATLGP HLHGVLMESI PADYVQTLHT VPVNPYSQYA LARSTTSLEW KISTLTNEAR QQIVGPINDA AFAGFRLRAS GIATQVTSRS LEQNPLSQFA RIFYARPETR KFRVEFLTPT AFKQSGEYVF WPDPRLVFQS LAQKYGAIVD GEEPDPGLIA EFGQSVRLSA FRVASAPFAV GAARVPGFTG SATFTVRGVD TFASYIAALL WFGEFSGCGI KASMGMGAIR VQPLAPREKC VPKP
NT Sequence
ttggctgctc gccgaggcgg catcagaagg acggatctgc tacgtcggag tggccagccc cgaggccgac atcgcgcgag tgcggcggaa tcaggcttga cctggatttc accaacgctt attctggtgg gcttttctca ccgaggagat cgccggatga cggaacactt gtcgcgattg acgctgactc tagaggttga tgccccgctc gaacgcgcga gggtggcgac cctggggccg catcttcatg gcgtcctcat ggagtcgatc ccggccgatt atgtgcagac cctccacacc gtgccggtga acccgtacag tcagtacgcg ctggcccgct cgaccacatc tttggagtgg aagatcagca cgctgacgaa cgaggcgcgg cagcagatcg tcggacctat caacgacgcg gcgtttgcgg gttttcggct ccgtgcgagc gggatagcga cacaggtcac gtcgcgatcg ctggagcaga acccgctaag tcaattcgcg cgcattttct acgcgcggcc cgagacgcgc aagttccggg tcgagttcct gacgcctacc gcattcaagc aatccggcga gtacgtgttc tggccggatc cgcggctcgt gtttcagagt ctcgcgcaga agtacggtgc aattgtcgac ggcgaagagc ccgatcctgg cctcatcgcc gaattcggtc agtcggttcg cctctccgcg ttccgggtgg cgtcggcccc gttcgcggtg ggcgcggcgc gtgttcccgg cttcaccggc tcggccacgt tcaccgtccg cggtgtggat acttttgcga gctatatcgc ggcgctgttg tggttcgggg agttctcggg atgcggaata aaggcatcca tggggatggg cgcgatccgg gtccagccac tggcaccgag ggaaaaatgc gtaccgaagc catga
Details for MytuD.18805.a.B1.GE37839
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML AARRGGIRRT DLLRRSGQPR GRHRASAAES GLTWISPTLI LVGFSHRGDR RMTEHLSRLT LTLEVDAPLE RARVATLGPH LHGVLMESIP ADYVQTLHTV PVNPYSQYAL ARSTTSLEWK ISTLTNEARQ QIVGPINDAA FAGFRLRASG IATQVTSRSL EQNPLSQFAR IFYARPETRK FRVEFLTPTA FKQSGEYVFW PDPRLVFQSL AQKYGAIVDG EEPDPGLIAE FGQSVRLSAF RVASAPFAVG AARVPGFTGS ATFTVRGVDT FASYIAALLW FGEFSGCGIK ASMGMGAIRV QPLAPRENAY RSHK
Validated NT Sequence
cnctagatan ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgttg gctgctcgcc gaggcggcat cagaaggacg gatctgctac gtcggagtgg ccagccccga ggccgacatc gcgcgagtgc ggcggaatca ggcttgacct ggatttcacc aacgcttatt ctggtgggct tttctcaccg aggagatcgc cggatgacgg aacacttgtc gcgattgacg ctgactctag aggttgatgc cccgctcgaa cgcgcgaggg tggcgaccct ggggccgcat cttcatggcg tcctcatgga gtcgatcccg gccgattatg tgcagaccct ccacaccgtg ccggtgaacc cgtacagtca gtacgcgctg gcccgctcga ccacatcttt ggagtggaag atcagcacgc tgacgaacga ggcgcggcag cagatcgtcg gacctatcaa cgacgcggcg tttgcgggtt ttcggctccg tgcgagcggg atagcgacac aggtcacgtc gcgatcgctg gagcagaacc cgctaagtca attcgcgcgc attttctacg cgcggcccga gacgcgcaag ttccgggtcg agttcctgac gcctaccgca ttcaagcaat ccggcgagta cgtgttctgg ccggatccgc ggctcgtgtt tcagagtctc gcgcagaagt acggtgcaat tgtcgacggc gaagagcccg atcctggcct catcgccgaa ttcggtcagt cggttcgcct ctccgcgttc cgggtggcgt cggccccgtt cgcggtgggc gcggcgcgtg ttcccggctt caccggctcg gccacgttca ccgtccgcgg tgtggatact tttgcgagct atatcgcggc gctgttgtgg ttcggggagt tctcgggatg cggaataaag gcatccatgg ggatgggcgc gatccgggtc cagccactgg caccgaggga aaatgcgtac cgaagccata agtgagtana tagatcnntg nnnnaanccc cccannnann nnnnnnnnnn nnnnnnnnnn nnnngncnnt ntcggagatn nnnncnggan gnnnnannnn nct
Expected Protein Sequence
MAHHHHHHMA ARRGGIRRTD LLRRSGQPRG RHRASAAESG LTWISPTLIL VGFSHRGDRR MTEHLSRLTL TLEVDAPLER ARVATLGPHL HGVLMESIPA DYVQTLHTVP VNPYSQYALA RSTTSLEWKI STLTNEARQQ IVGPINDAAF AGFRLRASGI ATQVTSRSLE QNPLSQFARI FYARPETRKF RVEFLTPTAF KQSGEYVFWP DPRLVFQSLA QKYGAIVDGE EPDPGLIAEF GQSVRLSAFR VASAPFAVGA ARVPGFTGSA TFTVRGVDTF ASYIAALLWF GEFSGCGIKA SMGMGAIRVQ PLAPREKCVP KP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggctg ctcgccgagg cggcatcaga aggacggatc tgctacgtcg gagtggccag ccccgaggcc gacatcgcgc gagtgcggcg gaatcaggct tgacctggat ttcaccaacg cttattctgg tgggcttttc tcaccgagga gatcgccgga tgacggaaca cttgtcgcga ttgacgctga ctctagaggt tgatgccccg ctcgaacgcg cgagggtggc gaccctgggg ccgcatcttc atggcgtcct catggagtcg atcccggccg attatgtgca gaccctccac accgtgccgg tgaacccgta cagtcagtac gcgctggccc gctcgaccac atctttggag tggaagatca gcacgctgac gaacgaggcg cggcagcaga tcgtcggacc tatcaacgac gcggcgtttg cgggttttcg gctccgtgcg agcgggatag cgacacaggt cacgtcgcga tcgctggagc agaacccgct aagtcaattc gcgcgcattt tctacgcgcg gcccgagacg cgcaagttcc gggtcgagtt cctgacgcct accgcattca agcaatccgg cgagtacgtg ttctggccgg atccgcggct cgtgtttcag agtctcgcgc agaagtacgg tgcaattgtc gacggcgaag agcccgatcc tggcctcatc gccgaattcg gtcagtcggt tcgcctctcc gcgttccggg tggcgtcggc cccgttcgcg gtgggcgcgg cgcgtgttcc cggcttcacc ggctcggcca cgttcaccgt ccgcggtgtg gatacttttg cgagctatat cgcggcgctg ttgtggttcg gggagttctc gggatgcgga ataaaggcat ccatggggat gggcgcgatc cgggtccagc cactggcacc gagggaaaaa tgcgtaccga agccatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat