MytuD.18804.a

Conserved protein (U0002kb)

CENTER ID: MytuD.18804.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18804.a.B1.GE37838 full length 1 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3296
RefSeq: NP_217472.1
RefSeq: YP_006516409.1
TubercuList: Rv2956
MTB Network Portal: Rv2956
UniProt: I6Y242

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSLKLARFI ARSAAFEVSR RYSERDLKHQ FVKQLKSRRV DVVFDVGANS GQYAAGLRRA AYKGRIVSFE PLSGPFTILE SKASTDPLWD CRQHALGDSD GTVTINIAGN AGQSSSVLPM LKSHQNAFPP ANYVGTQEAS IHRLDSVAPE FLGMNGVAFL KVDVQGFEKQ VLAGGKSTID DHCVGMQLEL SFLPLYEGGM LIPEALDLVY SLGFTLTGLL PCFIDANNGR MLQADGIFFR EDD
NT Sequence
gtgaaatcgt tgaaactcgc tcgtttcatc gcgcgtagcg ccgccttcga ggtttcgcgc cgctattctg agcgagacct gaagcaccag tttgtgaagc aactcaaatc gcgtcgggta gatgtcgttt tcgatgtcgg cgccaactca ggacaatacg ccgccggcct ccgccgagca gcatataagg gccgcattgt ctcgttcgaa ccgctatccg gaccgtttac gatcttggaa agcaaagcgt caacggatcc actttgggat tgccggcagc atgcgttggg cgattctgat ggaacggtta cgatcaatat cgcaggaaac gccggtcaga gcagttccgt cttgcccatg ctgaaaagtc atcagaacgc ttttcccccg gcaaactatg tcggtaccca agaggcgtcc atacatcgac ttgattccgt ggcgccagaa tttctaggca tgaacggtgt cgcttttctc aaggtcgacg ttcaaggctt tgaaaagcag gtgctcgccg ggggcaaatc aaccatagat gaccattgcg tcggcatgca actcgaactg tccttcctgc cgttgtacga aggtggcatg ctcattcctg aagccctcga tctcgtgtat tccttgggct tcacgttgac gggattgctg ccttgtttca ttgatgcaaa taatggtcga atgttgcagg ccgacggcat ctttttccgc gaggacgatt ga
Details for MytuD.18804.a.B1.GE37838
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV KSLKLARFIA RSAAFEVSRR YSERDLKHQF VKQLKSRRVD VVFDVGANSG QYAAGLRRAA YKGRIVSFEP LSGPFTILES KASTDPLWDC RQHALGDSDG TVTINIAGNA GQSSSVLPML KSHQNAFPPA NYVGTQEASI HRLDSVAPEF LGMNGVAFLK VDVQGFEKQV LAGGKSTIDD HCVGMQLELS FLPLYEGGML IPEALDLVYS LGFTLTGLLP CFIDANNGRM LQADGIFFRE DD
Validated NT Sequence
ctanannttt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatggtgaaa tcgttgaaac tcgctcgttt catcgcgcgt agcgccgcct tcgaggtttc gcgccgctat tctgagcgag acctgaagca ccagtttgtg aagcaactca aatcgcgtcg ggtagatgtc gttttcgatg tcggcgccaa ctcaggacaa tacgccgccg gcctccgccg agcagcatat aagggccgca ttgtctcgtt cgaaccgcta tccggaccgt ttacgatctt ggaaagcaaa gcgtcaacgg atccactttg ggattgccgg cagcatgcgt tgggcgattc tgatggaacg gttacgatca atatcgcagg aaacgccggt cagagcagtt ccgtcttgcc catgctgaaa agtcatcaga acgcttttcc cccggcaaac tatgtcggta cccaagaggc gtccatacat cgacttgatt ccgtggcgcc agaatttcta ggcatgaacg gtgtcgcttt tctcaaggtc gacgttcaag gctttgaaaa gcaggtgctc gccgggggca aatcaaccat agatgaccat tgcgtcggca tgcaactcga actgtccttc ctgccgttgt acgaaggtgg catgctcatt cctgaagccc tcgatctcgt gtattccttg ggcttcacgt tgacgggatt gctgccttgt ttcattgatg caaataatgg tcgaatgttg caggccgacg gcatcttttt ccgcgaggac gattaagtga gtaagatagg atccggctgn tnanancccc ccaaaanaag nnnnnaanng nnnaaaannt ngtnnnctnt cccaactgtc tgctactatn ncngctaaag tcnagccatt gctgtcatgn anaggaggcc gatgaatgat aangacgnat ctngagnnnc gnccnnncac nacnccagct acacncacct cacctnttnc cagantanta catcanagan ccnagctann atgacanggc nangnccnat tnncangnan gattnctnnt ntgnnacnnn ngngnatctn nna
Expected Protein Sequence
MAHHHHHHMK SLKLARFIAR SAAFEVSRRY SERDLKHQFV KQLKSRRVDV VFDVGANSGQ YAAGLRRAAY KGRIVSFEPL SGPFTILESK ASTDPLWDCR QHALGDSDGT VTINIAGNAG QSSSVLPMLK SHQNAFPPAN YVGTQEASIH RLDSVAPEFL GMNGVAFLKV DVQGFEKQVL AGGKSTIDDH CVGMQLELSF LPLYEGGMLI PEALDLVYSL GFTLTGLLPC FIDANNGRML QADGIFFRED D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaat cgttgaaact cgctcgtttc atcgcgcgta gcgccgcctt cgaggtttcg cgccgctatt ctgagcgaga cctgaagcac cagtttgtga agcaactcaa atcgcgtcgg gtagatgtcg ttttcgatgt cggcgccaac tcaggacaat acgccgccgg cctccgccga gcagcatata agggccgcat tgtctcgttc gaaccgctat ccggaccgtt tacgatcttg gaaagcaaag cgtcaacgga tccactttgg gattgccggc agcatgcgtt gggcgattct gatggaacgg ttacgatcaa tatcgcagga aacgccggtc agagcagttc cgtcttgccc atgctgaaaa gtcatcagaa cgcttttccc ccggcaaact atgtcggtac ccaagaggcg tccatacatc gacttgattc cgtggcgcca gaatttctag gcatgaacgg tgtcgctttt ctcaaggtcg acgttcaagg ctttgaaaag caggtgctcg ccgggggcaa atcaaccata gatgaccatt gcgtcggcat gcaactcgaa ctgtccttcc tgccgttgta cgaaggtggc atgctcattc ctgaagccct cgatctcgtg tattccttgg gcttcacgtt gacgggattg ctgccttgtt tcattgatgc aaataatggt cgaatgttgc aggccgacgg catctttttc cgcgaggacg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat