MytuD.18800.a

Uncharacterized protein

CENTER ID: MytuD.18800.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18800.a.B1.GE37835 full length 1 377
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.303
RefSeq: NP_214786.1
RefSeq: YP_006513596.1
TubercuList: Rv0272c
MTB Network Portal: Rv0272c
UniProt: P95229

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGRAATPGV IREFVGLPSR TAGRAAAGGH PCQGLYHHSV GRKPKVALIA AHYQIDFSEH YLAEYMAIRG IGFLGWNTRF RGFESSFLLD HALVDIGVGV RWLREVQGVE TVVLLGNSGG GSLMAAYQSQ AVDPNVTPLD GMRPAAGVTE LPAADAYVAA AAHPGRPDVL TAWMDAAVID ENDPVATDPE LDLFDERNGP PYSPEFISRY RSAQVKRNHT ITDWAESELK RVRAAGFSDR PFSVMRTWAD PRMVDPSIEP TKRRPNQCYA GTPVKANRSA HGIAAACTLR GWLGMWSLRV AQTRAAPHLA RITCPALVLN AEADTGIFPS DAQQIYDGLA SSDKTQVSID TDHYFTTPGA RSEQADTIAK WIAKRWR
NT Sequence
ttgactggtc gtgctgccac gcccggggtc atccgagagt tcgtcgggct gccgtcgcga accgcggggc gtgcggctgc cggtgggcac ccatgccagg ggctgtacca ccacagcgtg ggacgcaaac cgaaggtggc gctcatcgcc gcgcactacc agatcgattt ctccgagcac tatctcgccg agtacatggc gatccgcggc atcgggtttc tgggctggaa cacccgattc cgtggtttcg aaagcagctt tctgctcgat cacgcactgg tcgacatcgg cgtcggggtg cgctggctcc gcgaagttca gggcgtggaa acagtggtgt tgcttggcaa ttcaggtggt ggctcattga tggccgccta ccaatcacaa gcggtcgatc cgaacgtgac tcccctggac ggtatgcgac ccgcagccgg ggtaaccgaa ttgccggccg ccgacgcata cgttgccgcc gcagctcatc ccggccgtcc ggatgtgctc accgcttgga tggacgccgc cgtcattgac gaaaatgatc cggttgcaac cgatcccgaa cttgacctgt tcgacgaacg caacgggccg ccgtactcac cggagtttat cagccggtat cgctccgcgc aggtcaaacg caaccacacc atcaccgact gggccgaatc cgaactcaaa cgtgttcgcg cagctggctt ttctgatcgg ccgttcagcg tgatgcgtac ctgggccgac ccgcgcatgg tcgatcccag catcgaaccg accaagcgtc ggcccaacca gtgctacgcg ggcacgccgg tgaaggcgaa ccgctccgca cacggcatcg ccgcggcctg cacgctgcgc ggctggctgg gcatgtggag cctgcgggtg gcccaaaccc gtgccgcacc ccacctggcc cggatcacct gcccggccct ggtgctcaac gccgaggccg acaccggaat cttcccgtcg gatgcccagc agatctacga cgggctcgcc agcagcgaca agactcaggt ctcgatcgac accgaccatt acttcaccac cccgggcgcc cgcagcgagc aggccgacac catcgccaag tggatagcca agcggtggcg ctga
Details for MytuD.18800.a.B1.GE37835
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 96
Validated AA Sequence
MLTGRAATPG VIREFVGLPS RTAGRAAAGG HPCQGLYHHS VGRKPKVALI AAHYQIDFSE HYLAEYMAIR GIGFLGWNTR FRGFESSFLL DHALVDIGVG VRWLREVQGV ETVVLLGNSG GGSLMAAYQS QAVDPNVTPL DGMRPAAGVT ELPAADAYVA AAAHPGRPDV LTAWMDAAVI DENDPVATDP ELDLFDERNG PPYSPEFISR YRSAQVKRNH TITDWAESEL KRVRAAGFSD RPFSVMRTWA DPRMVDPSIE PTKRRPNQCY AGTPVKANRS AHGIAAACTL RGWLGMWSLR VAQTRAAPHL ARITCPALVL NAEADTXNLP VGCPADLRRA RQQRQDSGLD RHRPLLHHPG RPQRAGRHHR
Validated NT Sequence
ggcgatggtg tcggcctgct cgctgcgggc gcccggggtg gtgaagtaat ggtcggtgtc gatcgagacc tgagtcttgt cgctgctggc gagcccgtcg tagatctgct gggcatccga cgggaagatt ccnggtgtcg gcctcggcgt tgagcaccag ggccgggcag gtgatccggg ccaggtgggg tgcggcacgg gtttgggcca cccgcaggct ccacatgccc agccagccgc gcagcgtgca ggccgcggcg atgccgtgtg cggagcggtt cgccttcacc ggcgtgcccg cgtagcactg gttgggccga cgcttggtcg gttcgatgct gggatcgacc atgcgcgggt cggcccaggt acgcatcacg ctgaacggcc gatcagaaaa gccagctgcg cgaacacgtt tgagttcgga ttcggcccag tcggtgatgg tgtggttgcg tttgacctgc gcggagcgat accggctgat aaactccggt gagtacggcg gcccgttgcg ttcgtcgaac aggtcaagtt cgggatcggt tgcaaccgga tcattttcgt caatgacggc ggcgtccatc caagcggtga gcacatccgg acggccggga tgagctgcgg cggcaacgta tgcgtcggcg gccggcaatt cggttacccc ggctgcgggt cgcataccgt ccaggggagt cacgttcgga tcgaccgctt gtgattggta ggcggccatc aatgagccac cacctgaatt gccaagcaac accactgttt ccacgccctg aacttcgcgg agccagcgca ccccgacgcc gatgtcgacc agtgcgtgat cgagcagaaa gctgctttcg aaaccacgga atcgggtgtt ccagcccaga aacccgatgc cgcggatcgc catgtactcg gcgagatagt gctcggagaa atcgatctgg tagtgcgcgg cgatgagcgc caccttcggt ttgcgtccca cgctgtggtg gtacagcccc tggcatgggt gcccaccggc agccgcacgc cccgcggttc gcgacggcag cccgacgaac tctcggatga ccccgggcgt ggcagcacga ccagtcaaca tatggtggtg gtgg
Expected Protein Sequence
MAHHHHHHMT GRAATPGVIR EFVGLPSRTA GRAAAGGHPC QGLYHHSVGR KPKVALIAAH YQIDFSEHYL AEYMAIRGIG FLGWNTRFRG FESSFLLDHA LVDIGVGVRW LREVQGVETV VLLGNSGGGS LMAAYQSQAV DPNVTPLDGM RPAAGVTELP AADAYVAAAA HPGRPDVLTA WMDAAVIDEN DPVATDPELD LFDERNGPPY SPEFISRYRS AQVKRNHTIT DWAESELKRV RAAGFSDRPF SVMRTWADPR MVDPSIEPTK RRPNQCYAGT PVKANRSAHG IAAACTLRGW LGMWSLRVAQ TRAAPHLARI TCPALVLNAE ADTGIFPSDA QQIYDGLASS DKTQVSIDTD HYFTTPGARS EQADTIAKWI AKRWR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgactg gtcgtgctgc cacgcccggg gtcatccgag agttcgtcgg gctgccgtcg cgaaccgcgg ggcgtgcggc tgccggtggg cacccatgcc aggggctgta ccaccacagc gtgggacgca aaccgaaggt ggcgctcatc gccgcgcact accagatcga tttctccgag cactatctcg ccgagtacat ggcgatccgc ggcatcgggt ttctgggctg gaacacccga ttccgtggtt tcgaaagcag ctttctgctc gatcacgcac tggtcgacat cggcgtcggg gtgcgctggc tccgcgaagt tcagggcgtg gaaacagtgg tgttgcttgg caattcaggt ggtggctcat tgatggccgc ctaccaatca caagcggtcg atccgaacgt gactcccctg gacggtatgc gacccgcagc cggggtaacc gaattgccgg ccgccgacgc atacgttgcc gccgcagctc atcccggccg tccggatgtg ctcaccgctt ggatggacgc cgccgtcatt gacgaaaatg atccggttgc aaccgatccc gaacttgacc tgttcgacga acgcaacggg ccgccgtact caccggagtt tatcagccgg tatcgctccg cgcaggtcaa acgcaaccac accatcaccg actgggccga atccgaactc aaacgtgttc gcgcagctgg cttttctgat cggccgttca gcgtgatgcg tacctgggcc gacccgcgca tggtcgatcc cagcatcgaa ccgaccaagc gtcggcccaa ccagtgctac gcgggcacgc cggtgaaggc gaaccgctcc gcacacggca tcgccgcggc ctgcacgctg cgcggctggc tgggcatgtg gagcctgcgg gtggcccaaa cccgtgccgc accccacctg gcccggatca cctgcccggc cctggtgctc aacgccgagg ccgacaccgg aatcttcccg tcggatgccc agcagatcta cgacgggctc gccagcagcg acaagactca ggtctcgatc gacaccgacc attacttcac caccccgggc gcccgcagcg agcaggccga caccatcgcc aagtggatag ccaagcggtg gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat