SSGCID: Target Page

MytuD.18799.a

Conserved protein

CENTER ID:	MytuD.18799.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18799.a.B1.GE37834	full length		1	190

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.512
RefSeq:	NP_214978.1
RefSeq:	YP_006513793.1
TubercuList:	Rv0464c
MTB Network Portal:	Rv0464c
UniProt:	O53749

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGQNGQVAR ISPGKFRQLG PVNWLVAKLA ARAVGAPQMH LFTTLGYRQY LFWTFAIYTG RLLHGRLPGV DTELVILRVA HLRSCEYELQ HHRRMARRRG LDANTQATIF AWPDVPDGDG PRKVLSARQQ ALLQATDELI KDRTITAGTW ERLATHLDPR LLIEFCLLAT QYDAIAATIT ALAIPPDNPQ

NT Sequence

atgaccggtc agaacggcca agtcgcgcgt atctccccgg ggaagttccg ccagctgggg ccggtcaact ggctggtggc caagctggcc gcacgtgcgg ttggggcacc gcagatgcac ctgttcacca cgctgggcta ccgccagtac ttgttctgga cctttgccat ctacaccggc cgactactgc acgggcggct gcccggcgtc gataccgagt tggtgatcct tcgggtcgca cacctacgat cttgcgaata cgaacttcag catcatcgcc gaatggcgcg ccgtcggggc ctagacgcca acacccaggc cacgatattc gcctggcccg acgttccaga tggagacgga ccgcgaaaag tgctcagcgc ccgccaacag gcgctgctac aggctaccga tgaattgatc aaggatcgca cgatcaccgc gggcacctgg gagcggctgg caactcacct cgatccgcgc ctgttgatcg aattctgttt actcgcaacc caatacgacg cgatcgccgc aacgatcacc gccctggcca tcccgccgga caacccacag tag

Details for MytuD.18799.a.B1.GE37834

HARVESTED ON:	3/6/2014
SEQUENCED ON:	3/13/2014
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	76

Validated AA Sequence

MHLFTTLGYR QYLFWTFAIY TGRLLHGRLP GVDTELVILR VAHLRSCEYE LQHHRRMARR RGLDANTQAT IFAWPDVPDG DGPRKVLSAR QQALLQATDE LIKDRTITAG TWERLATHLD PRLLIEFCLL ATQYDAIAAT ITALAIPPDN PQ

Validated NT Sequence

ttagcagccg gatcctatct tactcactta ctgtgggttg tccggcggga tggccagggc ggtgatcgtt gcggcgatcg cgtcgtattg ggttgcgagt aaacagaatt cgatcaacag gcgcggatcg aggtgagttg ccagccgctc ccaggtgccc gcggtgatcg tgcgatcctt gatcaattca tcggtagcct gtagcagcgc ctgttggcgg gcgctgagca cttttcgcgg tccgtctcca tctggaacgt cgggccaggc gaatatcgtg gcctgggtgt tggcgtctag gccccgacgg cgcgccattc ggcgatgatg ctgaagttcg tattcgcaag atcgtaggtg tgcgacccga aggatcacca actcggtatc gacgccgggc agccgcccgt gcagtagtcg gccggtgtag atggcaaagg tccagaacaa gtactggcgg tagcccagcg tggtgaacag gtgcatctgc ggtgccccaa ccgcacgtgc ggccagcttg gccaccagcc agttgaccgg ccccagctgg cggaacttcc ccggggagat acgcgcgact tggccgttct gaccgg

Expected Protein Sequence

MAHHHHHHMT GQNGQVARIS PGKFRQLGPV NWLVAKLAAR AVGAPQMHLF TTLGYRQYLF WTFAIYTGRL LHGRLPGVDT ELVILRVAHL RSCEYELQHH RRMARRRGLD ANTQATIFAW PDVPDGDGPR KVLSARQQAL LQATDELIKD RTITAGTWER LATHLDPRLL IEFCLLATQY DAIAATITAL AIPPDNPQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg gtcagaacgg ccaagtcgcg cgtatctccc cggggaagtt ccgccagctg gggccggtca actggctggt ggccaagctg gccgcacgtg cggttggggc accgcagatg cacctgttca ccacgctggg ctaccgccag tacttgttct ggacctttgc catctacacc ggccgactac tgcacgggcg gctgcccggc gtcgataccg agttggtgat ccttcgggtc gcacacctac gatcttgcga atacgaactt cagcatcatc gccgaatggc gcgccgtcgg ggcctagacg ccaacaccca ggccacgata ttcgcctggc ccgacgttcc agatggagac ggaccgcgaa aagtgctcag cgcccgccaa caggcgctgc tacaggctac cgatgaattg atcaaggatc gcacgatcac cgcgggcacc tgggagcggc tggcaactca cctcgatccg cgcctgttga tcgaattctg tttactcgca acccaatacg acgcgatcgc cgcaacgatc accgccctgg ccatcccgcc ggacaaccca cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat