MytuD.18798.a

Putative trans-acting enoyl reductase Rv2449c (EC 1.3.1.-)

CENTER ID: MytuD.18798.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18798.a.B2.GE37832 soluble domain 1 1 283
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2745
RefSeq: NP_216965.1
RefSeq: YP_006515886.1
TubercuList: Rv2449c
MTB Network Portal: Rv2449c
UniProt: O53176

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTATPREFDI VLYGATGFVG KLTAEYLARA GGDARIALAG RSTQRVLAVR EALGESAQTW PILTADASLP STLQAMAARA QVVVTTVGPY TRYGLPLVAA CAAAGTDYAD LTGEPMFMRN SIDLYHKQAA DTGARIVHAC GFDSVPSDLS VYALYHAARE DGAGELTDTN CVVRSFKGGF SGGTIASMLE VLSTASNDPD ARRQLSDPYM LSPDRGAEPE LGPQPDLPSR RGRRLAPELA GVWTAGFIMA PTNTRIVRRS NALLDWAYGR RFRYSETMSV GSTVLAPVVS VVGGGVGNAM FGLASRYIRL LPRGLVKRVV PKPGTGPSAA ARERGYYRIE TYTTTTTGAR YLARMAQDGD PGYKATSVLL GECGLALALD RDKLSDMRGV LTPAAAMGDA LLERLPAAGV SLQTTRLAS
NT Sequence
gtgaccgcta cgccgcgtga attcgacatc gtgttgtacg gggcgaccgg cttcgtcggc aagctgaccg ccgaatacct ggcccgggcc gggggagatg cccggatcgc gctggccggc aggtcgacgc aacgggtgct cgccgttcgc gaagccctgg gcgaatccgc gcagacctgg ccgatcctga ccgccgacgc gtcgttgccg tcgacgctgc aagccatggc cgcccgcgcc caggtcgtgg tcaccaccgt tgggccctac acccgctacg ggctgccgct ggtggccgca tgcgctgccg ccggcaccga ctacgccgac ctgaccggcg agccgatgtt catgcgcaac agcatcgact tgtaccacaa gcaggccgcc gacaccggtg cacgcatcgt gcacgcctgc ggattcgact ccgtcccatc tgatctgagc gtgtacgccc tataccacgc ggcacgagaa gacggcgccg gcgagctgac cgacaccaac tgcgtggtgc gttccttcaa aggcgggttc tccggcggca ccatcgcatc gatgctggag gtgttgagca ccgcttctaa cgatcccgat gcgcgccgcc agctatccga cccctacatg ctgagcccag accgcggcgc cgaacccgaa ctgggcccac agcctgacct gccgtcgcgt cgtggccgtc ggctcgcgcc ggaactggcc ggtgtgtgga ccgcgggatt catcatggcg cccaccaaca cccggatcgt gcgccgcagc aacgcgctcc tggactgggc ctacggccgg cggttccgct acagcgaaac catgagcgta gggtccacgg tgctggcgcc ggtggtatcg gttgtcggcg gcggtgtcgg caacgcgatg ttcggcttgg ccagccgcta catccgcctg ctgccgcggg gactggtaaa gcgtgtcgtc cccaaacccg gcaccggccc cagtgcggcg gctcgcgagc gcggctacta ccgcatcgag acctacacca ccacaaccac cggagcccgg tacctggcgc gcatggccca agatggtgac cccggctaca aggcgacgtc ggtgttgttg ggggagtgtg gtctggcgct ggcactggat cgcgacaagc tgtccgacat gcgtggtgtg ttaacccccg cggccgcaat gggtgacgcc ctgctggagc gtttgccggc cgccggtgtg tcgctgcaga ccacgcgact ggctagctga
Details for MytuD.18798.a.B2.GE37832
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 31kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TATPREFDIV LYGATGFVGK LTAEYLARAG GDARIALAGR STQRVLAVRE ALGESAQTWP ILTADASLPS TLQAMAARAQ VVVTTVGPYT RYGLPLVAAC AAAGTDYADL TGEPMFMRNS IDLYHKQAAD TGARIVHACG FDSVPSDLSV YALYHAARED GAGELTDTNC VVRSFKGGFS GGTIASMLEV LSTASNDPDA RRQLSDPYML SPDRGAEPEL GPQPDLPSRR GRRLAPELAG VWTAGFIMAP TNTRIVRRSN ALLDWAYGRR FRYSETMSVG ST
Validated NT Sequence
annncnctan anaattttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat ggtgaccgct acgccgcgtg aattcgacat cgtgttgtac ggggcgaccg gcttcgtcgg caagctgacc gccgaatacc tggcccgggc cgggggagat gcccggatcg cgctggccgg caggtcgacg caacgggtgc tcgccgttcg cgaagccctg ggcgaatccg cgcagacctg gccgatcctg accgccgacg cgtcgttgcc gtcgacgctg caagccatgg ccgcccgcgc ccaggtcgtg gtcaccaccg ttgggcccta cacccgctac gggctgccgc tggtggccgc atgcgctgcc gccggcaccg actacgccga cctgaccggc gagccgatgt tcatgcgcaa cagcatcgac ttgtaccaca agcaggccgc cgacaccggt gcacgcatcg tgcacgcctg cggattcgac tccgtcccat ctgatctgag cgtgtacgcc ctataccacg cggcacgaga agacggcgcc ggcgagctga ccgacaccaa ctgcgtggtg cgttccttca aaggcgggtt ctccggcggc accatcgcat cgatgctgga ggtgttgagc accgcttcta acgatcccga tgcgcgccgc cagctatccg acccctacat gctgagccca gaccgcggcg ccgaacccga actgggccca cagcctgacc tgccgtcgcg tcgtggccgt cggctcgcgc cggaactggc cggtgtgtgg accgcgggat tcatcatggc gcccaccaac acccggatcg tgcgccgcag caacgcgctc ctggactggg cctacggccg gcggttccgc tacagcgaaa ccatgagcgt agggtccacg taagtgagta agataggatc ccgct
Expected Protein Sequence
MAHHHHHHMT ATPREFDIVL YGATGFVGKL TAEYLARAGG DARIALAGRS TQRVLAVREA LGESAQTWPI LTADASLPST LQAMAARAQV VVTTVGPYTR YGLPLVAACA AAGTDYADLT GEPMFMRNSI DLYHKQAADT GARIVHACGF DSVPSDLSVY ALYHAAREDG AGELTDTNCV VRSFKGGFSG GTIASMLEVL STASNDPDAR RQLSDPYMLS PDRGAEPELG PQPDLPSRRG RRLAPELAGV WTAGFIMAPT NTRIVRRSNA LLDWAYGRRF RYSETMSVGS T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg ctacgccgcg tgaattcgac atcgtgttgt acggggcgac cggcttcgtc ggcaagctga ccgccgaata cctggcccgg gccgggggag atgcccggat cgcgctggcc ggcaggtcga cgcaacgggt gctcgccgtt cgcgaagccc tgggcgaatc cgcgcagacc tggccgatcc tgaccgccga cgcgtcgttg ccgtcgacgc tgcaagccat ggccgcccgc gcccaggtcg tggtcaccac cgttgggccc tacacccgct acgggctgcc gctggtggcc gcatgcgctg ccgccggcac cgactacgcc gacctgaccg gcgagccgat gttcatgcgc aacagcatcg acttgtacca caagcaggcc gccgacaccg gtgcacgcat cgtgcacgcc tgcggattcg actccgtccc atctgatctg agcgtgtacg ccctatacca cgcggcacga gaagacggcg ccggcgagct gaccgacacc aactgcgtgg tgcgttcctt caaaggcggg ttctccggcg gcaccatcgc atcgatgctg gaggtgttga gcaccgcttc taacgatccc gatgcgcgcc gccagctatc cgacccctac atgctgagcc cagaccgcgg cgccgaaccc gaactgggcc cacagcctga cctgccgtcg cgtcgtggcc gtcggctcgc gccggaactg gccggtgtgt ggaccgcggg attcatcatg gcgcccacca acacccggat cgtgcgccgc agcaacgcgc tcctggactg ggcctacggc cggcggttcc gctacagcga aaccatgagc gtagggtcca cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat