MytuD.18796.a

Conserved protein

CENTER ID: MytuD.18796.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18796.a.B1.GE37830 full length 1 344
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2410
RefSeq: NP_216675.1
RefSeq: YP_006515578.1
TubercuList: Rv2159c
MTB Network Portal: Rv2159c
UniProt: O06218

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFVNHIEPV APRRAGGAVA EVYAEARREF GRLPEPLAML SPDEGLLTAG WATLRETLLV GQVPRGRKEA VAAAVAASLR CPWCVDAHTT MLYAAGQTDT AAAILAGTAP AAGDPNAPYV AWAAGTGTPA GPPAPFGPDV AAEYLGTAVQ FHFIARLVLV LLDETFLPGG PRAQQLMRRA GGLVFARKVR AEHRPGRSTR RLEPRTLPDD LAWATPSEPI ATAFAALSHH LDTAPHLPPP TRQVVRRVVG SWHGEPMPMS SRWTNEHTAE LPADLHAPTR LALLTGLAPH QVTDDDVAAA RSLLDTDAAL VGALAWAAFT AARRIGTWIG AAAEGQVSRQ NPTG
NT Sequence
atgaaatttg tcaaccatat tgagcccgtc gcgccccgcc gagccggcgg cgcggtcgcc gaggtctatg ccgaggcccg ccgcgagttc ggccggctgc ccgagccgct cgccatgctg tccccggacg agggactgct caccgccggc tgggcgacgt tgcgcgagac actgctggtg ggccaggtgc cgcgtggccg caaggaagcc gtcgccgccg ccgtcgcggc cagcctgcgc tgcccctggt gcgtcgacgc acacaccacc atgctgtacg cggcaggcca aaccgacacc gccgcggcga tcttggccgg cacagcacct gccgccggtg acccgaacgc gccgtatgtg gcgtgggcgg caggaaccgg gacaccggcg ggaccgccgg caccgttcgg cccggatgtc gccgccgaat acctgggcac cgcggtgcaa ttccacttca tcgcacgcct ggtcctggtg ctgctggacg aaaccttcct gccggggggc ccgcgcgccc aacagctcat gcgccgcgcc ggtggactgg tgttcgcccg caaggtgcgc gcggagcatc ggccgggccg ctccacccgc cggctcgagc cgcgaacgct gcccgacgat ctggcatggg caacaccgtc cgagcccata gcaaccgcgt tcgccgcgct cagccaccac ctggacaccg cgccgcacct gccgccaccg actcgtcagg tggtcaggcg ggtcgtgggg tcgtggcacg gcgagccaat gccgatgagc agtcgctgga cgaacgagca caccgccgag ctgcccgccg acctgcacgc gcccacccgt cttgccctgc tgaccggcct ggccccgcat caggtgaccg acgacgacgt cgccgcggcc cgatccctgc tcgacaccga tgcggcgctg gttggcgccc tggcctgggc cgccttcacc gccgcgcggc gcatcggcac ctggatcggc gccgccgccg agggccaggt gtcgcggcaa aacccgactg ggtga
Details for MytuD.18796.a.B1.GE37830
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 86
Validated AA Sequence
MLSPDEGLLT AGWATLRETL LVGQVPRGRK EAVAAAVAAS LRCPWCVDAH TTMLYAAGQT DTAAAILAGT APAAGDPNAP YVAWAAGTGT PAGPPAPFGP DVAAEYLGTA VQFHFIARLV LVLLDETFLP GGPRAQQLMR RAGGLVFARK VRAEHRPGRS TRRLEPRTLP DDLAWATPSE PIATAFAALS HHLDTAPHLP PPTRQVVRRV VGSWHGEPMP MSSRWTNEHT AELPADLHAP TRLALLTGLA PHQVTDDDVA AARSLLDTDA ALVGALAWAA FTAARRIGTW IGAAAEGQVS RQNPTG
Validated NT Sequence
atcttactca cttacccagt cgggttttgc cgcgacacct ggccctcggc ggcggcgccg atccaggtgc cgatgcgccg cgcggcggtg aaggcggccc aggccagggc gccaaccagc gccgcatcgg tgtcgagcag ggatcgggcc gcggcgacgt cgtcgtcggt cacctgatgc ggggccaggc cggtcagcag ggcaagacgg gtgggcgcgt gcaggtcggc gggcagctcg gcggtgtgct cgttcgtcca gcgactgctc atcggcattg gctcgccgtg ccacgacccc acgacccgcc tgaccacctg acgagtcggt ggcggcaggt gcggcgcggt gtccaggtgg tggctgagcg cggcgaacgc ggttgctatg ggctcggacg gtgttgccca tgccagatcg tcgggcagcg ttcgcggctc gagccggcgg gtggagcggc ccggccgatg ctccgcgcgc accttgcggg cgaacaccag tccaccggcg cggcgcatga gctgttgggc gcgcgggccc cccggcagga aggtttcgtc cagcagcacc aggaccaggc gtgcgatgaa gtggaattgc accgcggtgc ccaggtattc ggcggcgaca tccgggccga acggtgccgg cggtcccgcc ggtgtcccgg ttcctgccgc ccacgccaca tacggcgcgt tcgggtcacc ggcggcaggt gctgtgccgg ccaagatcgc cgcggcggtg tcggtttggc ctgccgcgta cagcatggtg gtgtgtgcgt cgacgcacca ggggcagcgc aggctggccg cgacggcggc ggcgacggct tccttgcggc cacgcggcac ctggcccacc agcagtgtct cgcgcaacgt cgcccagccg gcggtgagca gtccctcgtc cggggacagc atggcgagcg gctcgggcag ccggccgaac tcgcggcggg cctcggcata gacctcggcg accgcgccgc cggctcggcg gggcgcgacg ggctcaatat ggttgacaaa ttt
Expected Protein Sequence
MAHHHHHHMK FVNHIEPVAP RRAGGAVAEV YAEARREFGR LPEPLAMLSP DEGLLTAGWA TLRETLLVGQ VPRGRKEAVA AAVAASLRCP WCVDAHTTML YAAGQTDTAA AILAGTAPAA GDPNAPYVAW AAGTGTPAGP PAPFGPDVAA EYLGTAVQFH FIARLVLVLL DETFLPGGPR AQQLMRRAGG LVFARKVRAE HRPGRSTRRL EPRTLPDDLA WATPSEPIAT AFAALSHHLD TAPHLPPPTR QVVRRVVGSW HGEPMPMSSR WTNEHTAELP ADLHAPTRLA LLTGLAPHQV TDDDVAAARS LLDTDAALVG ALAWAAFTAA RRIGTWIGAA AEGQVSRQNP TG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat ttgtcaacca tattgagccc gtcgcgcccc gccgagccgg cggcgcggtc gccgaggtct atgccgaggc ccgccgcgag ttcggccggc tgcccgagcc gctcgccatg ctgtccccgg acgagggact gctcaccgcc ggctgggcga cgttgcgcga gacactgctg gtgggccagg tgccgcgtgg ccgcaaggaa gccgtcgccg ccgccgtcgc ggccagcctg cgctgcccct ggtgcgtcga cgcacacacc accatgctgt acgcggcagg ccaaaccgac accgccgcgg cgatcttggc cggcacagca cctgccgccg gtgacccgaa cgcgccgtat gtggcgtggg cggcaggaac cgggacaccg gcgggaccgc cggcaccgtt cggcccggat gtcgccgccg aatacctggg caccgcggtg caattccact tcatcgcacg cctggtcctg gtgctgctgg acgaaacctt cctgccgggg ggcccgcgcg cccaacagct catgcgccgc gccggtggac tggtgttcgc ccgcaaggtg cgcgcggagc atcggccggg ccgctccacc cgccggctcg agccgcgaac gctgcccgac gatctggcat gggcaacacc gtccgagccc atagcaaccg cgttcgccgc gctcagccac cacctggaca ccgcgccgca cctgccgcca ccgactcgtc aggtggtcag gcgggtcgtg gggtcgtggc acggcgagcc aatgccgatg agcagtcgct ggacgaacga gcacaccgcc gagctgcccg ccgacctgca cgcgcccacc cgtcttgccc tgctgaccgg cctggccccg catcaggtga ccgacgacga cgtcgccgcg gcccgatccc tgctcgacac cgatgcggcg ctggttggcg ccctggcctg ggccgccttc accgccgcgc ggcgcatcgg cacctggatc ggcgccgccg ccgagggcca ggtgtcgcgg caaaacccga ctgggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat