MytuD.18792.a

Conserved protein

CENTER ID: MytuD.18792.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18792.a.B1.GE37828 full length 1 428
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1977
RefSeq: NP_216286.1
RefSeq: YP_006515170.1
TubercuList: Rv1770
MTB Network Portal: Rv1770
UniProt: O06803

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDEAHPAHPA DAGRPGGPIQ GARRGAAMTP ITALPTELAA MREVVETLAP IERAAGEPGE HKAAEWIVER LRTAGAQDAR IEEEQYLDGY PRLHLKLSVI GVAAGVAGLL SRRLRIPAAL AGVGAGLAIA DDCANGPRIV RKRTETPRTT WNAVAEAGDP AGQLTVVVCA HHDAAHSGKF FEAHIEEVMV ELFPGIVERI DTQLPNWWGP ILAPALAGVG ALRGSRPMMI AGTVGSALAA ALFADIARSP VVPGANDNLS AVALLVALAE RLRERPVKGV RVLLVSLGAE ETLQGGIYGF LARHKPELDR DRTYFLNFDT IGSPELIMLE GEGPTVMEDY FYRPFRDLVI RAAERADAPL RRGIRSRNST DAVLMSRAGY PTACFVSINR HKSVANYHLM SDTPENLCYE TVSHAVTVAE SVIRELAR
NT Sequence
atggacgagg cccacccggc tcacccggca gatgcggggc ggcccggtgg cccaattcaa ggcgcgcgaa gaggagctgc catgacaccg atcaccgccc tgccgaccga gttggcggcc atgcgcgagg tagtcgagac gctcgcaccc attgagcgtg ccgcgggcga gccgggtgag cacaaggcgg ccgagtggat cgtcgagcgc ctgcgcacgg cgggcgcgca ggacgcgcgc atcgaggagg agcagtacct cgacggctac ccgaggctgc acctcaagct gtcggtgatc ggggtggcgg ccggcgtcgc gggcctgctc agcagacgtt tgcgcatccc cgccgcgctg gccggggtgg gtgcggggct ggcaatcgcc gacgattgcg ccaacgggcc gcgcattgtg cgcaaacgaa cggagacgcc ccggacgaca tggaacgcgg tagccgaggc cggtgatcct gctggtcagc taacagttgt tgtgtgcgct caccacgacg ccgcgcacag cggcaagttt ttcgaggctc atattgagga ggtaatggtc gagctgtttc ccgggattgt ggagcgcatc gacacgcagc tgccgaactg gtgggggccg atcctcgcgc ccgcactcgc cggtgtcggc gccctgcgcg gcagccggcc gatgatgatc gccggaacgg tgggtagcgc cctggccgcc gctttgttcg ccgacatcgc gcgcagtccg gtcgtccccg gtgccaacga caatctctcc gcggttgcgc tgctggtcgc gctggccgag cggctgcgcg agcggccggt gaagggcgtg cgagtgttgc tcgtgtccct gggggccgag gaaacgttgc agggcgggat ctacgggttc ctggcgcgac acaaacccga gctggaccgc gaccgcacat acttcctgaa cttcgacacc atcggctcac ccgagctcat catgctcgag ggcgagggcc cgacggtcat ggaggactac ttctatcggc cattccggga tctggtcatc cgggcggccg agcgcgccga cgcgccgctg cggcgcggca tccggtcgcg caacagtacc gacgcggtgt tgatgagccg cgccggctac ccgaccgcgt gctttgtgtc gatcaaccgg cacaagtcgg tggccaatta ccacctgatg tccgatacac ctgagaatct ctgctatgag acggtgtccc acgccgtcac cgtcgccgaa tccgtgatca gggagctggc ccgatga
Details for MytuD.18792.a.B1.GE37828
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMD EAHPAHPADA GRPGGPIQGA RRGAAMTPIT ALPTELAAMR EVVETLAPIE RAAGEPGEHK AAEWIVERLR TAGAQDARIE EEQYLDGYPR LHLKLSVIGV AAGVAGLLSR RLRIPAALAG VGAGLAIADD CANGPRIVRK RTETPRTTWN AVAEAGDPAG QLTVVVCAHH DAAHSGKFFE AHIEEVMVEL FPGIVERIDT QLPNWWGPIL APALAGVGAL RGSRPMMIAG TVGSALAAAL FADIARSPVV PGANDNLSAV ALLVALAERL RERPVKGRAS VARVPGGRGN VAGGIYGFLA RHKPELDRDR TYFLNFDTIG XPELIMLEGE GPTVMEDYXI GIPGSGHPXX SAXTXXXXXX XRAXXXXXX
Validated NT Sequence
cnnnnnnntc nctananntt ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatggacgag gcccacccgg ctcacccggc agatgcgggg cggcccggtg gcccaattca aggcgcgcga agaggagctg ccatgacacc gatcaccgcc ctgccgaccg agttggcggc catgcgcgag gtagtcgaga cgctcgcacc cattgagcgt gccgcgggcg agccgggtga gcacaaggcg gccgagtgga tcgtcgagcg cctgcgcacg gcgggcgcgc aggacgcgcg catcgaggag gagcagtacc tcgacggcta cccgaggctg cacctcaagc tgtcggtgat cggggtggcg gccggcgtcg cgggcctgct cagcagacgt ttgcgcatcc ccgccgcgct ggccggggtg ggtgcggggc tggcaatcgc cgacgattgc gccaacgggc cgcgcattgt gcgcaaacga acggagacgc cccggacgac atggaacgcg gtagccgagg ccggtgatcc tgctggtcag ctaacagttg ttgtgtgcgc tcaccacgac gccgcgcaca gcggcaagtt tttcgaggct catattgagg aggtaatggt cgagctgttt cccgggattg tggagcgcat cgacacgcag ctgccgaact ggtgggggcc gatcctcgcg cccgcactcg ccggtgtcgg cgccctgcgc ggcagccggc cgatgatgat cgccggaacg gtgggtagcg ccctggccgc cgctttgttc gccgacatcg cgcgcagtcc ggtcgtcccc ggtgccaacg acaatctctc cgcggttgcg ctgctggtcg cgctggccga gcggctgcgc gagcggccgg tgaaagggcg tgcgagtgtt gctcgtgtcc ctgggggccg aggaaacgtt gcaggcggga tctacgggtt cctggcgcga cacaaacccg agctggaccg cgaccgcaca tacttcctga acttcgacac catcggctna cccgagctca tcatgctcga gggcgagggc ccgacggtca tggaggacta ctntatcggc attccgggat ctggtcatcc nnnnnnnagc gcgncgacgc nncnctncng nnnnnatcnn cgcgcaanng nacnacnnng gnnttgatnn nnnnnnnnta cccnannnnn nnnnnttngt cgatnnnnnn nannnnnntg canntaccng ngangttccn naatncancn nnanaannnn ngc
Expected Protein Sequence
MAHHHHHHMD EAHPAHPADA GRPGGPIQGA RRGAAMTPIT ALPTELAAMR EVVETLAPIE RAAGEPGEHK AAEWIVERLR TAGAQDARIE EEQYLDGYPR LHLKLSVIGV AAGVAGLLSR RLRIPAALAG VGAGLAIADD CANGPRIVRK RTETPRTTWN AVAEAGDPAG QLTVVVCAHH DAAHSGKFFE AHIEEVMVEL FPGIVERIDT QLPNWWGPIL APALAGVGAL RGSRPMMIAG TVGSALAAAL FADIARSPVV PGANDNLSAV ALLVALAERL RERPVKGVRV LLVSLGAEET LQGGIYGFLA RHKPELDRDR TYFLNFDTIG SPELIMLEGE GPTVMEDYFY RPFRDLVIRA AERADAPLRR GIRSRNSTDA VLMSRAGYPT ACFVSINRHK SVANYHLMSD TPENLCYETV SHAVTVAESV IRELAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg aggcccaccc ggctcacccg gcagatgcgg ggcggcccgg tggcccaatt caaggcgcgc gaagaggagc tgccatgaca ccgatcaccg ccctgccgac cgagttggcg gccatgcgcg aggtagtcga gacgctcgca cccattgagc gtgccgcggg cgagccgggt gagcacaagg cggccgagtg gatcgtcgag cgcctgcgca cggcgggcgc gcaggacgcg cgcatcgagg aggagcagta cctcgacggc tacccgaggc tgcacctcaa gctgtcggtg atcggggtgg cggccggcgt cgcgggcctg ctcagcagac gtttgcgcat ccccgccgcg ctggccgggg tgggtgcggg gctggcaatc gccgacgatt gcgccaacgg gccgcgcatt gtgcgcaaac gaacggagac gccccggacg acatggaacg cggtagccga ggccggtgat cctgctggtc agctaacagt tgttgtgtgc gctcaccacg acgccgcgca cagcggcaag tttttcgagg ctcatattga ggaggtaatg gtcgagctgt ttcccgggat tgtggagcgc atcgacacgc agctgccgaa ctggtggggg ccgatcctcg cgcccgcact cgccggtgtc ggcgccctgc gcggcagccg gccgatgatg atcgccggaa cggtgggtag cgccctggcc gccgctttgt tcgccgacat cgcgcgcagt ccggtcgtcc ccggtgccaa cgacaatctc tccgcggttg cgctgctggt cgcgctggcc gagcggctgc gcgagcggcc ggtgaagggc gtgcgagtgt tgctcgtgtc cctgggggcc gaggaaacgt tgcagggcgg gatctacggg ttcctggcgc gacacaaacc cgagctggac cgcgaccgca catacttcct gaacttcgac accatcggct cacccgagct catcatgctc gagggcgagg gcccgacggt catggaggac tacttctatc ggccattccg ggatctggtc atccgggcgg ccgagcgcgc cgacgcgccg ctgcggcgcg gcatccggtc gcgcaacagt accgacgcgg tgttgatgag ccgcgccggc tacccgaccg cgtgctttgt gtcgatcaac cggcacaagt cggtggccaa ttaccacctg atgtccgata cacctgagaa tctctgctat gagacggtgt cccacgccgt caccgtcgcc gaatccgtga tcagggagct ggcccgatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at