SSGCID: Target Page

MytuD.18789.a

Conserved protein

CENTER ID:	MytuD.18789.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18789.a.B1.GE37827	full length		1	300

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2006
RefSeq:	NP_216310.1
RefSeq:	YP_006515194.1
TubercuList:	Rv1794
MTB Network Portal:	Rv1794
UniProt:	O53943

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDQQSTRTDI TVNVDGFWML QALLDIRHVA PELRCRPYVS TDSNDWLNEH PGMAVMREQG IVVNDAVNEQ VAARMKVLAA PDLEVVALLS RGKLLYGVID DENQPPGSRD IPDNEFRVVL ARRGQHWVSA VRVGNDITVD DVTVSDSASI AALVMDGLES IHHADPAAIN AVNVPMEEML EATKSWQESG FNVFSGGDLR RMGISAATVA ALGQALSDPA AEVAVYARQY RDDAKGPSAS VLSLKDGSGG RIALYQQART AGSGEAWLAI CPATPQLVQV GVKTVLDTLP YGEWKTHSRV

NT Sequence

atggatcaac agagtacccg caccgacatc accgtcaacg tcgacggctt ctggatgctt caggcgctac tggatatccg ccacgttgcg cctgagttac gttgccggcc gtacgtctcc accgattcca atgactggct aaacgagcac ccggggatgg cggtcatgcg cgagcagggc attgtcgtca acgacgcggt caacgaacag gtcgctgccc ggatgaaggt gcttgccgca cctgatcttg aagtcgtcgc cctgctgtca cgcggcaagt tgctgtacgg ggtcatagac gacgagaacc agccgccggg ttcgcgtgac atccctgaca atgagttccg ggtggtgttg gcccggcgag gccagcactg ggtgtcggcg gtacgggttg gcaatgacat caccgtcgat gacgtgacgg tctcggatag cgcctcgatc gccgcactgg taatggacgg tctggagtcg attcaccacg ccgacccagc cgcgatcaac gcggtcaacg tgccaatgga ggagatgcta gaggcaacga agtcgtggca ggaatcgggg tttaacgtct tctccggcgg agatctgcgc cgaatgggca tcagtgccgc gacggtggcc gcgctggggc aggcgttgtc ggatcccgcg gccgaggtcg cagtgtatgc gcgacagtac cgagacgacg ccaagggccc cagcgcctcg gtgttgtcgc tgaaagacgg ctccggtgga cgcatcgcgc tgtatcagca ggcgcgaacg gcaggttccg gcgaggcgtg gctggctatc tgcccggcta ccccgcagtt ggtgcaagta ggagtgaaga ccgttttgga tacactgccc tacggcgagt ggaaaacaca cagcagagta tga

Details for MytuD.18789.a.B1.GE37827

HARVESTED ON:	3/6/2014
SEQUENCED ON:	3/13/2014
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD QQSTRTDITV NVDGFWMLQA LLDIRHVAPE LRCRPYVSTD SNDWLNEHPG MAVMREQGIV VNDAVNEQVA ARMKVLAAPD LEVVALLSRG KLLYGVIDDE NQPPGSRDIP DNEFRVVLAR RGQHWVSAVR VGNDITVDDV TVSDSASIAA LVMDGLESIH HADPAAINAV NVPMEEMLEA TKSWQESGFN VFSGGDLRRM GISAATVAAL GQALSDPAAE VAVYARQYRD DAKGPSASVL SLKDGSGGRI ALYQQARTAG SGEAWLAICP ATPQLVQVGV KTVLDTLPYG EWKTHSRV

Validated NT Sequence

atcttactca cttatactct gctgtgtgtt ttccactcgc cgtagggcag tgtatccaaa acggtcttca ctcctacttg caccaactgc ggggtagccg ggcagatagc cagccacgcc tcgccggaac ctgccgttcg cgcctgctga tacagcgcga tgcgtccacc ggagccgtct ttcagcgaca acaccgaggc gctggggccc ttggcgtcgt ctcggtactg tcgcgcatac actgcgacct cggccgcggg atccgacaac gcctgcccca gcgcggccac cgtcgcggca ctgatgccca ttcggcgcag atctccgccg gagaagacgt taaaccccga ttcctgccac gacttcgttg cctctagcat ctcctccatt ggcacgttga ccgcgttgat cgcggctggg tcggcgtggt gaatcgactc cagaccgtcc attaccagtg cggcgatcga ggcgctatcc gagaccgtca cgtcatcgac ggtgatgtca ttgccaaccc gtaccgccga cacccagtgc tggcctcgcc gggccaacac cacccggaac tcattgtcag ggatgtcacg cgaacccggc ggctggttct cgtcgtctat gaccccgtac agcaacttgc cgcgtgacag cagggcgacg acttcaagat caggtgcggc aagcaccttc atccgggcag cgacctgttc gttgaccgcg tcgttgacga caatgccctg ctcgcgcatg accgccatcc ccgggtgctc gtttagccag tcattggaat cggtggagac gtacggccgg caacgtaact caggcgcaac gtggcggata tccagtagcg cctgaagcat ccagaagccg tcgacgttga cggtgatgtc ggtgcgggta ctctgttgat ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc aacagagtac ccgcaccgac atcaccgtca acgtcgacgg cttctggatg cttcaggcgc tactggatat ccgccacgtt gcgcctgagt tacgttgccg gccgtacgtc tccaccgatt ccaatgactg gctaaacgag cacccgggga tggcggtcat gcgcgagcag ggcattgtcg tcaacgacgc ggtcaacgaa caggtcgctg cccggatgaa ggtgcttgcc gcacctgatc ttgaagtcgt cgccctgctg tcacgcggca agttgctgta cggggtcata gacgacgaga accagccgcc gggttcgcgt gacatccctg acaatgagtt ccgggtggtg ttggcccggc gaggccagca ctgggtgtcg gcggtacggg ttggcaatga catcaccgtc gatgacgtga cggtctcgga tagcgcctcg atcgccgcac tggtaatgga cggtctggag tcgattcacc acgccgaccc agccgcgatc aacgcggtca acgtgccaat ggaggagatg ctagaggcaa cgaagtcgtg gcaggaatcg gggtttaacg tcttctccgg cggagatctg cgccgaatgg gcatcagtgc cgcgacggtg gccgcgctgg ggcaggcgtt gtcggatccc gcggccgagg tcgcagtgta tgcgcgacag taccgagacg acgccaaggg ccccagcgcc tcggtgttgt cgctgaaaga cggctccggt ggacgcatcg cgctgtatca gcaggcgcga acggcaggtt ccggcgaggc gtggctggct atctgcccgg ctaccccgca gttggtgcaa gtaggagtga agaccgtttt ggatacactg ccctacggcg agtggaaaac acacagcaga gtatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat