MytuD.18783.a

Conserved protein

CENTER ID: MytuD.18783.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18783.a.B1.GE37933 full length 1 563
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3448
RefSeq: NP_217607.1
RefSeq: YP_006516553.1
TubercuList: Rv3091
MTB Network Portal: Rv3091
UniProt: I6YB49

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPIPFADGML SRLGRRGAAL DLIEEFEDES GEPPASLSPA DLLAAEPALL LQKMENRLVR HHLANPDVLS GEQLRKLRYI LNFARLADFE PGAAGPGGSR GRGDISVGGQ VAPWRSRVVD ALYAPLREEP DPVTALEGAK DVLATLVDDQ DDQRRVLIER HGSDFSATEL DAEVGYKKLV TVLGGGGGAG FVYIGGMQRL LAAGQVPDYM IGSSFGSIIG SLVARELPVP IDEYAEWAKT VSYRAILGPE RRRSRHGLAG MFTLRFDQFA HTLLSRADGE RMRMSDLAIP FDVVVAGVRR QPYAALPSRF RHRERSTLTL RSLPFLPIGI GPWVAARMWQ VAAFIDLRVV KPIVISADGA TRDVNVVDAA SFSSAIPGVL HHETSDPRML PILDELCADQ DVAAMVDGGA ASNVPVELAW ERVRDGRLGT RNACYLAFDC FHPHWDPRHL WLVPITQAVQ LQMVRNLPYA DHLVRFEPTL SPVNLAPSAA AIDRACRWGR DSVEPAIAVT SALLEPTWWE GDRPPAAEPK ERTKSAASSM SAVMAAIQAP TGRFRRWRSR HLT
NT Sequence
atgccgatcc cctttgccga tgggatgctc agccggctgg gtcgccgcgg ggcagcgctc gacctgatcg aggagttcga ggacgagtcc ggggagcccc ccgcatccct gagccccgcc gacctgctgg ccgccgaacc ggccctgctg ctgcagaaga tggagaaccg cctcgtccgg caccacctag ccaatccgga cgtgttgagc ggcgaacagc tgcgcaagct gcgctacatc ctcaatttcg ccaggctggc cgacttcgaa ccgggggccg cggggccggg cggaagccgc ggtcgcgggg acatctcggt gggcggccaa gtcgcgcctt ggcggtcccg ggtcgtcgac gcgttgtacg caccgctgcg cgaggagccc gatccggtca cggcgctgga gggcgcgaaa gacgtgctgg cgacgctggt cgacgaccag gacgatcagc gtcgagtgct catcgagcgc cacggcagcg acttctccgc gacggaactc gacgccgagg tcggctacaa gaagctggtg accgtcctcg gcggcggcgg gggcgcgggc ttcgtctaca tcggcggcat gcaacggctg ctggcggccg gccaggtgcc cgactacatg atcggctcgt cgttcgggtc gatcatcggc agcctggtgg cccgtgaact gccggtgccg atcgacgagt acgccgagtg ggccaaaacg gtgtcctacc gcgccatcct gggcccggag cggcggcgca gccgccacgg gttggccgga atgttcaccc tgcgcttcga ccagttcgcc cataccctgc tcagccgtgc ggacggcgaa cggatgcgca tgtcggatct ggcaatcccg ttcgatgtcg tcgtcgccgg tgtgcgcagg cagccttatg cggcgctgcc gtccaggttc cgccatcgcg agcggtctac actgacgttg cggtcgctgc cgtttctgcc gatcggtatc ggcccgtggg tggcggcacg catgtggcaa gtcgcggcct tcatcgactt gcgggtggtc aagccgatcg tcatcagcgc cgacggcgcg acacgcgacg tcaacgtcgt tgacgcggcg tctttctcgt cggccatccc cggtgtgctg caccacgaaa ccagcgaccc gcggatgctg ccaatcctcg acgagttgtg cgccgaccag gacgtcgcgg cgatggtcga cggcggcgcg gccagcaacg tcccggtcga attggcgtgg gagcgggtcc gcgacgggcg gctcggcacc cgcaacgcgt gttatctggc gttcgactgc ttccatccgc actgggaccc ccgacatctg tggctggtac cgatcaccca ggcggtccag ctgcagatgg tgcgcaacct gccctacgcc gaccacctcg tccgattcga gccgacgctg tcgccggtga acctggcgcc gtccgcggcg gccatcgacc gggcttgccg gtgggggcgc gacagcgtcg aaccggcgat tgcggtgaca tcggcgctgc tggagccgac gtggtgggaa ggcgacaggc cccccgccgc cgaacccaag gaacgcacaa agtcggcggc ctcgtcgatg agcgccgtga tggccgcgat tcaggcgccg acgggccggt ttcggcgatg gcgaagccgc cacctgacct ag
Details for MytuD.18783.a.B1.GE37933
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 63kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 40
Validated AA Sequence
MAHHHHHHMP IPFADGMLSR LGRRGAALDL IEEFEDESGE PPASLSPADL LAAEPALLLQ KMENRLVRHH LANPDVLSGE QLRKLRYILN FARLADFEPG AAGPGGSRGR GDISVGGQVA PWRSRVVDAL YAPLREEPDP VTALEGAKDV LATLVDDQDD QRRVLIERHG SDFSATELDA EVGYKKLVTV PRRXRGRGLR LHRRHATAAG GRPGARLHDR LVVRVDHRQP GGP
Validated NT Sequence
cnnntnnana attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgccg atcccctttg ccgatgggat gctcagccgg ctgggtcgcc gcggggcagc gctcgacctg atcgaggagt tcgaggacga gtccggggag ccccccgcat ccctgagccc cgccgacctg ctggccgccg aaccggccct gctgctgcag aagatggaga accgcctcgt ccggcaccac ctagccaatc cggacgtgtt gagcggcgaa cagctgcgca agctgcgcta catcctcaat ttcgccaggc tggccgactt cgaaccgggg gccgcggggc cgggcggaag ccgcggtcgc ggggacatct cggtgggcgg ccaagtcgcg ccttggcggt cccgggtcgt cgacgcgttg tacgcaccgc tgcgcgagga gcccgatccg gtcacggcgc tggagggcgc gaaagacgtg ctggcgacgc tggtcgacga ccaggacgat cagcgtcgag tgctcatcga gcgccacggc agcgacttct ccgcgacgga actcgacgcc gaggtcggct acaagaagct ggtgaccgtc cctcggcggn ggcgggggcg cgggcttcgt ctacatcggc ggcatgcaac ggctgctggc ggccggccag gtgcccgact acatgatcgg ctcgtcgttc gggtcgatca tcggcagcct ggtggcccgt gaactgcccg gtgccgatcg acgagtacgc cgagtgggcc aaaacggtgt cctaccgtgc catccctggg ctcggagcgg cggcgcatct ctctactggt tggccctgaa tgttcaccnt gcgcttcgac ccagttcgcc cataccctgc tcaacccgtg cggacggnna agggaagcgt angncgnatc tggnaatccn ttcnatgtcg tcntnctcgg tgngcgcagg cagncttatc ngtgctgctg tctgngttcn ccacnnaagc tgtctnanct gacgtgcngt cnnttgccgt tttctgccgt atcggnatcn gtccgnggng ggctgncacg nnngnggnca agncgnnggt ccttntntcn nanttgctgg nnggttaann ntaatcnncc tntacncnnc ctaannggtc gcnnnnnngn nnac
Expected Protein Sequence
MAHHHHHHMP IPFADGMLSR LGRRGAALDL IEEFEDESGE PPASLSPADL LAAEPALLLQ KMENRLVRHH LANPDVLSGE QLRKLRYILN FARLADFEPG AAGPGGSRGR GDISVGGQVA PWRSRVVDAL YAPLREEPDP VTALEGAKDV LATLVDDQDD QRRVLIERHG SDFSATELDA EVGYKKLVTV LGGGGGAGFV YIGGMQRLLA AGQVPDYMIG SSFGSIIGSL VARELPVPID EYAEWAKTVS YRAILGPERR RSRHGLAGMF TLRFDQFAHT LLSRADGERM RMSDLAIPFD VVVAGVRRQP YAALPSRFRH RERSTLTLRS LPFLPIGIGP WVAARMWQVA AFIDLRVVKP IVISADGATR DVNVVDAASF SSAIPGVLHH ETSDPRMLPI LDELCADQDV AAMVDGGAAS NVPVELAWER VRDGRLGTRN ACYLAFDCFH PHWDPRHLWL VPITQAVQLQ MVRNLPYADH LVRFEPTLSP VNLAPSAAAI DRACRWGRDS VEPAIAVTSA LLEPTWWEGD RPPAAEPKER TKSAASSMSA VMAAIQAPTG RFRRWRSRHL T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccga tcccctttgc cgatgggatg ctcagccggc tgggtcgccg cggggcagcg ctcgacctga tcgaggagtt cgaggacgag tccggggagc cccccgcatc cctgagcccc gccgacctgc tggccgccga accggccctg ctgctgcaga agatggagaa ccgcctcgtc cggcaccacc tagccaatcc ggacgtgttg agcggcgaac agctgcgcaa gctgcgctac atcctcaatt tcgccaggct ggccgacttc gaaccggggg ccgcggggcc gggcggaagc cgcggtcgcg gggacatctc ggtgggcggc caagtcgcgc cttggcggtc ccgggtcgtc gacgcgttgt acgcaccgct gcgcgaggag cccgatccgg tcacggcgct ggagggcgcg aaagacgtgc tggcgacgct ggtcgacgac caggacgatc agcgtcgagt gctcatcgag cgccacggca gcgacttctc cgcgacggaa ctcgacgccg aggtcggcta caagaagctg gtgaccgtcc tcggcggcgg cgggggcgcg ggcttcgtct acatcggcgg catgcaacgg ctgctggcgg ccggccaggt gcccgactac atgatcggct cgtcgttcgg gtcgatcatc ggcagcctgg tggcccgtga actgccggtg ccgatcgacg agtacgccga gtgggccaaa acggtgtcct accgcgccat cctgggcccg gagcggcggc gcagccgcca cgggttggcc ggaatgttca ccctgcgctt cgaccagttc gcccataccc tgctcagccg tgcggacggc gaacggatgc gcatgtcgga tctggcaatc ccgttcgatg tcgtcgtcgc cggtgtgcgc aggcagcctt atgcggcgct gccgtccagg ttccgccatc gcgagcggtc tacactgacg ttgcggtcgc tgccgtttct gccgatcggt atcggcccgt gggtggcggc acgcatgtgg caagtcgcgg ccttcatcga cttgcgggtg gtcaagccga tcgtcatcag cgccgacggc gcgacacgcg acgtcaacgt cgttgacgcg gcgtctttct cgtcggccat ccccggtgtg ctgcaccacg aaaccagcga cccgcggatg ctgccaatcc tcgacgagtt gtgcgccgac caggacgtcg cggcgatggt cgacggcggc gcggccagca acgtcccggt cgaattggcg tgggagcggg tccgcgacgg gcggctcggc acccgcaacg cgtgttatct ggcgttcgac tgcttccatc cgcactggga cccccgacat ctgtggctgg taccgatcac ccaggcggtc cagctgcaga tggtgcgcaa cctgccctac gccgaccacc tcgtccgatt cgagccgacg ctgtcgccgg tgaacctggc gccgtccgcg gcggccatcg accgggcttg ccggtggggg cgcgacagcg tcgaaccggc gattgcggtg acatcggcgc tgctggagcc gacgtggtgg gaaggcgaca ggccccccgc cgccgaaccc aaggaacgca caaagtcggc ggcctcgtcg atgagcgccg tgatggccgc gattcaggcg ccgacgggcc ggtttcggcg atggcgaagc cgccacctga cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat