MytuD.18778.a

Conserved protein

CENTER ID: MytuD.18778.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18778.a.B1.GE37929 full length 1 327
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3390
RefSeq: NP_217554.1
RefSeq: YP_006516498.1
TubercuList: Rv3038c
MTB Network Portal: Rv3038c
UniProt: I6YAZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRSSNIPAD ATPNPHATAE QVAAARHDSK LAQVLYHDWE AENYDEKWSI SYDQRCVDYA RGRFDAIVPD EVIAQLPYDR ALELGCGTGF FLLNLIQAGV ARRGSVTDLS PGMVKVATRN GQALGLDIDG RVADAEGIPY DDDAFDLVVG HAVLHHIPDV ELSLREVVRV LKPGGRFVFA GEPTTVGDGY ARTLSTLTWR VVTNATKLPG LRGWRRPQGE LDESSRAAAL EALVDLHTFT PQDLQRIAHN AGAVEVQTAT EEFTAAMLGW PLRTFECTVP PGRLGWGWAR FAFTSWKTLG WVDANVWRHV VPKGWFYNVM ITGVKPS
NT Sequence
atgacgagga gttcaaacat ccccgccgac gccactccca atccacatgc cacggccgag caggtggccg cagctcggca cgacagcaag ctcgcccagg tgctctacca cgactgggaa gccgagaact acgacgagaa gtggtcgatc tcttatgacc agcgttgcgt cgactacgcc cgcggccggt tcgacgcgat cgtgcctgat gaggtcatcg cgcagctgcc ctacgaccgc gcactggaac tgggctgtgg aaccggattc ttcctgctca acttgatcca ggctggcgtt gcccggcgtg gatcggtcac cgacctctca cccggcatgg tcaaggtcgc cacccgcaat gggcaagcgc tggggctaga catcgacggc cgggtcgccg acgccgaggg catcccgtac gacgacgacg ctttcgacct cgtggtcggg catgcggtgc tgcaccacat tcccgacgtc gagctgtcgt tgcgggaggt ggttcgggta ctcaagccgg gcgggcgctt cgtgttcgcc ggcgagccga ccaccgtcgg cgacggctac gctcgcacac tgtccacgct gacctggcgt gtcgtcacca acgctaccaa gctgcccggc ctgagaggct ggcgccgacc acaaggcgag ctcgatgagt cctcgcgcgc cgcggcgctg gaggccctgg tcgacctgca cacattcacg ccccaggacc tgcagcggat cgcgcacaac gcgggtgcag tcgaggtcca aaccgccacc gaggagttca ccgcggcgat gctgggttgg ccgttgcgca cgtttgagtg cacggtgccg ccagggcggc tgggctgggg ttgggcgcgc ttcgccttca ccagctggaa gactctgggc tgggtggacg ccaacgtctg gcggcatgtg gtgccgaagg gctggttcta caacgtgatg attaccgggg ttaagccgtc ctga
Details for MytuD.18778.a.B1.GE37929
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMT RSSNIPADAT PNPHATAEQV AAARHDSKLA QVLYHDWEAE NYDEKWSISY DQRCVDYARG RFDAIVPDEV IAQLPYDRAL ELGCGTGFFL LNLIQAGVAR RGSVTDLSPG MVKVATRNGQ ALGLDIDGRV ADAEGIPYDD DAFDLVVGHA VLHHIPDVEL SLREVVRVLK PGGRFVFAGE PTTVGDGYAR TLSTLTWRVV TNATKLPGLR GWRRPQGELD ESSRAAALEA LVDLHTFTPX DLQRIAHNAG AVEVQTATEE FTAAMLGWPL RTFECTVPPG GWAGVGRASP SPAGRLWAGW TPRLAACXPX LXYX
Validated NT Sequence
cnnnnnnnnn nnncnctana nnnttttgtt tactttaaga aggagatata ccatggctca ccaccaccac caccatatga cgaggagttc aaacatcccc gccgacgcca ctcccaatcc acatgccacg gccgagcagg tggccgcagc tcggcacgac agcaagctcg cccaggtgct ctaccacgac tgggaagccg agaactacga cgagaagtgg tcgatctctt atgaccagcg ttgcgtcgac tacgcccgcg gccggttcga cgcgatcgtg cctgatgagg tcatcgcgca gctgccctac gaccgcgcac tggaactggg ctgtggaacc ggattcttcc tgctcaactt gatccaggct ggcgttgccc ggcgtggatc ggtcaccgac ctctcacccg gcatggtcaa ggtcgccacc cgcaatgggc aagcgctggg gctagacatc gacggccggg tcgccgacgc cgagggcatc ccgtacgacg acgacgcttt cgacctcgtg gtcgggcatg cggtgctgca ccacattccc gacgtcgagc tgtcgttgcg ggaggtggtt cgggtactca agccgggcgg gcgcttcgtg ttcgccggcg agccgaccac cgtcggcgac ggctacgctc gcacactgtc cacgctgacc tggcgtgtcg tcaccaacgc taccaagctg cccggcctga gaggctggcg ccgaccacaa ggcgagctcg atgagtcctc gcgcgccgcg gcgctggagg ccctggtcga cctgcacaca ttcacgcccc angacctgca gcggatcgcg cacaacgcgg gtgcagtcga ggtccaaacc gccaccgagg agttcaccgc ggcgatgctg ggttggccgt tgcgcacgtt tgagtgcacg gtgccgccag gcggctgggc tggggttggg cgcgcttcgc cttcaccagc tgggagactc tgggctgggt ggacgccacg tctggcggca tgtgngccgn nnctggnnta canntgatga ttaccggggt aagccgtcct agnngagtaa gataggatcg ntgctnnaaa ncccccaaa
Expected Protein Sequence
MAHHHHHHMT RSSNIPADAT PNPHATAEQV AAARHDSKLA QVLYHDWEAE NYDEKWSISY DQRCVDYARG RFDAIVPDEV IAQLPYDRAL ELGCGTGFFL LNLIQAGVAR RGSVTDLSPG MVKVATRNGQ ALGLDIDGRV ADAEGIPYDD DAFDLVVGHA VLHHIPDVEL SLREVVRVLK PGGRFVFAGE PTTVGDGYAR TLSTLTWRVV TNATKLPGLR GWRRPQGELD ESSRAAALEA LVDLHTFTPQ DLQRIAHNAG AVEVQTATEE FTAAMLGWPL RTFECTVPPG RLGWGWARFA FTSWKTLGWV DANVWRHVVP KGWFYNVMIT GVKPS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga ggagttcaaa catccccgcc gacgccactc ccaatccaca tgccacggcc gagcaggtgg ccgcagctcg gcacgacagc aagctcgccc aggtgctcta ccacgactgg gaagccgaga actacgacga gaagtggtcg atctcttatg accagcgttg cgtcgactac gcccgcggcc ggttcgacgc gatcgtgcct gatgaggtca tcgcgcagct gccctacgac cgcgcactgg aactgggctg tggaaccgga ttcttcctgc tcaacttgat ccaggctggc gttgcccggc gtggatcggt caccgacctc tcacccggca tggtcaaggt cgccacccgc aatgggcaag cgctggggct agacatcgac ggccgggtcg ccgacgccga gggcatcccg tacgacgacg acgctttcga cctcgtggtc gggcatgcgg tgctgcacca cattcccgac gtcgagctgt cgttgcggga ggtggttcgg gtactcaagc cgggcgggcg cttcgtgttc gccggcgagc cgaccaccgt cggcgacggc tacgctcgca cactgtccac gctgacctgg cgtgtcgtca ccaacgctac caagctgccc ggcctgagag gctggcgccg accacaaggc gagctcgatg agtcctcgcg cgccgcggcg ctggaggccc tggtcgacct gcacacattc acgccccagg acctgcagcg gatcgcgcac aacgcgggtg cagtcgaggt ccaaaccgcc accgaggagt tcaccgcggc gatgctgggt tggccgttgc gcacgtttga gtgcacggtg ccgccagggc ggctgggctg gggttgggcg cgcttcgcct tcaccagctg gaagactctg ggctgggtgg acgccaacgt ctggcggcat gtggtgccga agggctggtt ctacaacgtg atgattaccg gggttaagcc gtcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat