MytuD.18776.a

Uncharacterized protein Rv2000/MT2056

CENTER ID: MytuD.18776.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18776.a.B1.GE37927 full length 1 537
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2234
RefSeq: NP_216516.1
RefSeq: YP_006515411.1
TubercuList: Rv2000
MTB Network Portal: Rv2000
UniProt: P9WLN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPGFVGLGF GQWPVYVVRW PKLHLTPRQR KRVLHRRRLL TDRPISLSQI PIRTGGPMND PWPRPTQGPA KTIETDYLVI GAGAMGMAFT DTLITESGAR VVMIDRACQP GGHWTTAYPF VRLHQPSAYY GVNSRALGNN TIDLVGWNQG LNELAPVGEI CAYFDAVLQQ QLLPTGRVDY FPMSEYLGDG RFRTLAGTEY VVTVNRRIVD ATYLRAVVPS MRPAPYSVAP GVDCVAPNEL PKLGTRDRYV VVGAGKTGMD VCLWLLRNDV CPDKLTWIMP RDSWLIDRAT LQPGPTFVRQ FRESYGATLE AIGAATSTDD LFDRLETAGT LLRIDPSVRP SMYRCATVSH LELEQLRRIR DIVRMGHVQR IEPTTIVLDG GSVPATPTAL YIDCTADGAP QRPAKPVFDA DHLTLQAVRG CQQVFSAAFI AHVEFAYEDD AVKNELCTPI PHPDCDLDWM RLMHSDLGNF QRWLNDPDLT DWLSSARLNL LADLLPPLSH KPRVRERVVS MFQKRLGTAG DQLAKLLDAA TATTEQR
NT Sequence
ttgacgcccg agacccgcat gcgtccaggg tttgtgggtt tggggtttgg tcagtggccg gtctacgttg ttcgctggcc taaactccac ctgacgccgc ggcagcgaaa gcgtgtcttg catcggcgac gattgctcac cgatcgcccg atttcgttgt cacaaattcc aatccgcaca ggagggccca tgaacgaccc gtggcccagg ccaacgcaag ggccggcgaa aaccatcgaa accgactacc tggtgatagg tgccggagcg atgggaatgg cattcacgga taccctcatc accgagtccg gtgcgcgcgt cgtcatgatc gaccgcgcat gtcaacctgg tggacattgg accaccgcct acccgttcgt gcggctacac cagccatcgg cctattacgg cgtcaactca agggcactag gcaacaacac cattgacctc gtcggttgga accagggact gaacgaactg gcaccagtcg gcgagatatg cgcctacttc gatgctgtat tgcagcagca actgctcccc accgggcggg ttgactactt cccgatgagc gaatacctgg gcgacggccg gttccggaca ctggcaggca ccgaatacgt cgtcaccgtc aatcggcgca tcgtcgatgc cacctacctg cgtgccgtcg taccgtcgat gcggccggcg ccgtactcgg ttgcacccgg cgtcgactgc gtcgctccaa acgaactgcc caaactcggc acccgggatc gctacgtggt cgtcggtgcc ggcaagaccg gcatggacgt ctgcctatgg ttgctccgaa acgacgtctg ccctgacaag ctgacctgga tcatgccgcg tgattcctgg ctgatcgacc gagcgacgct gcagcccggg cccacattcg tcaggcagtt cagggaaagc tacggtgcga ctctcgaggc catcggggcc gcgacctcga ccgacgatct gttcgaccga ctagagaccg ccggaaccct gctgcgcatc gacccctcgg tgcgtccgag catgtatcgc tgcgccactg tgtcgcacct cgaactcgag cagctgcgcc gtatccgcga catcgtcagg atgggccacg tccaacgcat cgagcccacc acgatagtgc tcgacggcgg atcggttccc gccacaccca cggccctcta tattgactgc accgccgatg gagcaccaca acgtccagcc aagccggttt tcgacgcaga ccacctaacc ctgcaagccg tgcgcggatg ccaacaggtg ttcagcgccg cgtttatcgc gcacgtcgaa ttcgcctacg aggacgacgc ggtgaaaaac gaactctgta ccccgattcc acacccggac tgcgatctgg actggatgcg tctgatgcac tccgatctag gcaactttca gcgctggtta aacgaccccg atctgacgga ctggctgagc tcggcgcggt tgaacttgct cgccgacctg ctgccgccgt tgtctcacaa gccgcgggtg cgcgagcggg tggtgtcgat gttccaaaag aggttgggca ccgccggcga ccagctagcg aagctgctcg acgccgccac cgcaacaacc gaacaacgct aa
Details for MytuD.18776.a.B1.GE37927
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 61kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHML TPETRMRPGF VGLGFGQWPV YVVRWPKLHL TPRQRKRVLH RRRLLTDRPI SLSQIPIRTG GPMNDPWPRP TQGPAKTIET DYLVIGAGAM GMAFTDTLIT ESGARVVMID RACQPGGHWT TAYPFVRLHQ PSAYYGVNSR ALGNNTIDLV GWNQGLNELA PVGEICAYFD AVLQQQLLPT GRVDYFPMSE YLGDGRFRTL AGTEYVVTVN RRIVDATYLR AVVPSMRPAP YSVAPGVDCV APNELPKLGT RDRYVVVGAG KTGMDVCLWL LRNDVCPDKL TWIMPRDSWL IDRATLQPGP TFVRQFRKAX XDSRGHRGRD LDRRSVRRLE TAGTLLRIDP SVRPSMYRCX XXRXLXXXXC XXPXXXQDGX VXHXXXXXXT RIXPXXXXXX XXXXXXX
Validated NT Sequence
cnctagaata ttttgtttac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga cgcccgagac ccgcatgcgt ccagggtttg tgggtttggg gtttggtcag tggccggtct acgttgttcg ctggcctaaa ctccacctga cgccgcggca gcgaaagcgt gtcttgcatc ggcgacgatt gctcaccgat cgcccgattt cgttgtcaca aattccaatc cgcacaggag ggcccatgaa cgacccgtgg cccaggccaa cgcaagggcc ggcgaaaacc atcgaaaccg actacctggt gataggtgcc ggagcgatgg gaatggcatt cacggatacc ctcatcaccg agtccggtgc gcgcgtcgtc atgatcgacc gcgcatgtca acctggtgga cattggacca ccgcctaccc gttcgtgcgg ctacaccagc catcggccta ttacggcgtc aactcaaggg cactaggcaa caacaccatt gacctcgtcg gttggaacca gggactgaac gaactggcac cagtcggcga gatatgcgcc tacttcgatg ctgtattgca gcagcaactg ctccccaccg ggcgggttga ctacttcccg atgagcgaat acctgggcga cggccggttc cggacactgg caggcaccga atacgtcgtc accgtcaatc ggcgcatcgt cgatgccacc tacctgcgtg ccgtcgtacc gtcgatgcgg ccggcgccgt actcggttgc acccggcgtc gactgcgtcg ctccaaacga actgcccaaa ctcggcaccc gggatcgcta cgtggtcgtc ggtgccggca agaccggcat ggacgtctgc ctatggttgc tccgaaacga cgtctgccct gacaagctga cctggatcat gccgcgtgat tcctggctga tcgaccgagc gacgctgcag cccgggccca cattcgtcag gcagttcagg aaagctannn gcgactctcg aggccatcgg ggccgcgacc tcgaccgacg atctgttcga cgactagaga ccgccggaac cctgctgcgc atcgacccct cggtgcgtcc gagcatgtat cgctgcncnn tgnnncgcnn cctcnnnncg ancnnctgcn nnnntccncn acntcntcag gatgggcnng tcnnncatnn nnnncncgan nnnnnnacgc gnatcnnncc nncnnnncnc annnntnttn tnnttgnnnc gnnnnnnnna a
Expected Protein Sequence
MAHHHHHHMR PGFVGLGFGQ WPVYVVRWPK LHLTPRQRKR VLHRRRLLTD RPISLSQIPI RTGGPMNDPW PRPTQGPAKT IETDYLVIGA GAMGMAFTDT LITESGARVV MIDRACQPGG HWTTAYPFVR LHQPSAYYGV NSRALGNNTI DLVGWNQGLN ELAPVGEICA YFDAVLQQQL LPTGRVDYFP MSEYLGDGRF RTLAGTEYVV TVNRRIVDAT YLRAVVPSMR PAPYSVAPGV DCVAPNELPK LGTRDRYVVV GAGKTGMDVC LWLLRNDVCP DKLTWIMPRD SWLIDRATLQ PGPTFVRQFR ESYGATLEAI GAATSTDDLF DRLETAGTLL RIDPSVRPSM YRCATVSHLE LEQLRRIRDI VRMGHVQRIE PTTIVLDGGS VPATPTALYI DCTADGAPQR PAKPVFDADH LTLQAVRGCQ QVFSAAFIAH VEFAYEDDAV KNELCTPIPH PDCDLDWMRL MHSDLGNFQR WLNDPDLTDW LSSARLNLLA DLLPPLSHKP RVRERVVSMF QKRLGTAGDQ LAKLLDAATA TTEQR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgacgc ccgagacccg catgcgtcca gggtttgtgg gtttggggtt tggtcagtgg ccggtctacg ttgttcgctg gcctaaactc cacctgacgc cgcggcagcg aaagcgtgtc ttgcatcggc gacgattgct caccgatcgc ccgatttcgt tgtcacaaat tccaatccgc acaggagggc ccatgaacga cccgtggccc aggccaacgc aagggccggc gaaaaccatc gaaaccgact acctggtgat aggtgccgga gcgatgggaa tggcattcac ggataccctc atcaccgagt ccggtgcgcg cgtcgtcatg atcgaccgcg catgtcaacc tggtggacat tggaccaccg cctacccgtt cgtgcggcta caccagccat cggcctatta cggcgtcaac tcaagggcac taggcaacaa caccattgac ctcgtcggtt ggaaccaggg actgaacgaa ctggcaccag tcggcgagat atgcgcctac ttcgatgctg tattgcagca gcaactgctc cccaccgggc gggttgacta cttcccgatg agcgaatacc tgggcgacgg ccggttccgg acactggcag gcaccgaata cgtcgtcacc gtcaatcggc gcatcgtcga tgccacctac ctgcgtgccg tcgtaccgtc gatgcggccg gcgccgtact cggttgcacc cggcgtcgac tgcgtcgctc caaacgaact gcccaaactc ggcacccggg atcgctacgt ggtcgtcggt gccggcaaga ccggcatgga cgtctgccta tggttgctcc gaaacgacgt ctgccctgac aagctgacct ggatcatgcc gcgtgattcc tggctgatcg accgagcgac gctgcagccc gggcccacat tcgtcaggca gttcagggaa agctacggtg cgactctcga ggccatcggg gccgcgacct cgaccgacga tctgttcgac cgactagaga ccgccggaac cctgctgcgc atcgacccct cggtgcgtcc gagcatgtat cgctgcgcca ctgtgtcgca cctcgaactc gagcagctgc gccgtatccg cgacatcgtc aggatgggcc acgtccaacg catcgagccc accacgatag tgctcgacgg cggatcggtt cccgccacac ccacggccct ctatattgac tgcaccgccg atggagcacc acaacgtcca gccaagccgg ttttcgacgc agaccaccta accctgcaag ccgtgcgcgg atgccaacag gtgttcagcg ccgcgtttat cgcgcacgtc gaattcgcct acgaggacga cgcggtgaaa aacgaactct gtaccccgat tccacacccg gactgcgatc tggactggat gcgtctgatg cactccgatc taggcaactt tcagcgctgg ttaaacgacc ccgatctgac ggactggctg agctcggcgc ggttgaactt gctcgccgac ctgctgccgc cgttgtctca caagccgcgg gtgcgcgagc gggtggtgtc gatgttccaa aagaggttgg gcaccgccgg cgaccagcta gcgaagctgc tcgacgccgc cacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat