SSGCID: Target Page

MytuD.18775.a

Uncharacterized protein

CENTER ID:	MytuD.18775.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18775.a.B1.GE37926	full length		1	236

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3929
RefSeq:	NP_218036.1
RefSeq:	YP_006517007.1
TubercuList:	Rv3519
MTB Network Portal:	Rv3519
UniProt:	O53564

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPVSQHTIAG TVLTMPVRIR TANLHSAMFS VPADPAQRLI DYSGLRVCEY LPGKAIVMQM LVRYVDGDLG RYHEYGTAIM VNPPGTQRRG PRALTRAAAF IHHLPVDQVF TLEAGRTIWG FPKIMADFNV TDGRRFGFDV SADGRLIAGI EFSTGLPVPT LGWQMLKTYS HHDGVTREIP WEMKVSGLRA RLGGARLRLG DHPYAKELAS LGLPKRALLS QSAANVEMTF GDGHPI

NT Sequence

atgcccgtct cgcaacacac catcgccggc acggtgctca ccatgccggt gcgcattcgc accgccaacc tgcattccgc gatgttctcg gtgcccgccg acccagcgca gcgcctcatc gactacagcg ggctgcgggt gtgcgaatac ctgcccggta aggcaatcgt gatgcagatg ctggtgcgct acgtcgacgg ggatttgggg cgataccacg agtacggcac cgcgatcatg gtgaacccgc ccggcaccca acgccgcggg cccagagccc tcacccgagc cgccgcgttc atccatcatc tgccggtaga tcaggtgttc acgcttgagg ccgggcgcac catctggggc ttcccgaaga tcatggcgga cttcaacgtc accgacggcc ggaggttcgg cttcgacgtc agcgccgacg gacggttgat cgccgggatc gagttcagca ccggcctgcc ggtgccgacc ctcgggtggc aaatgttgaa gacctactcc caccatgacg gcgtaactcg cgagattccc tgggaaatga aagtctcggg cctgcgcgcc cggctcggcg gcgcccgact gcggttggga gaccatccct acgccaaaga actggcatcg ctgggcctgc cgaagcgggc tctgttgtcc cagtcggcgg ccaacgtaga aatgaccttc ggcgacggtc acccgatctg a

Details for MytuD.18775.a.B1.GE37926

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP VSQHTIAGTV LTMPVRIRTA NLHSAMFSVP ADPAQRLIDY SGLRVCEYLP GKAIVMQMLV RYVDGDLGRY HEYGTAIMVN PPGTQRRGPR ALTRAAAFIH HLPVDQVFTL EAGRTIWGFP KIMADFNVTD GRRFGFDVSA DGRLIAGIEF STGLPVPTLG WQMLKTYSHH DGVTREIPWE MKVSGLRARL GGARLRLGDH PYAKELASLG LPKRALLSQS AANVEMTFGD GHPI

Validated NT Sequence

tcnctanata attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatgcc cgtctcgcaa cacaccatcg ccggcacggt gctcaccatg ccggtgcgca ttcgcaccgc caacctgcat tccgcgatgt tctcggtgcc cgccgaccca gcgcagcgcc tcatcgacta cagcgggctg cgggtgtgcg aatacctgcc cggtaaggca atcgtgatgc agatgctggt gcgctacgtc gacggggatt tggggcgata ccacgagtac ggcaccgcga tcatggtgaa cccgcccggc acccaacgcc gcgggcccag agccctcacc cgagccgccg cgttcatcca tcatctgccg gtagatcagg tgttcacgct tgaggccggg cgcaccatct ggggcttccc gaagatcatg gcggacttca acgtcaccga cggccggagg ttcggcttcg acgtcagcgc cgacggacgg ttgatcgccg ggatcgagtt cagcaccggc ctgccggtgc cgaccctcgg gtggcaaatg ttgaagacct actcccacca tgacggcgta actcgcgaga ttccctggga aatgaaagtc tcgggcctgc gcgcccggct cggcggcgcc cgactgcggt tgggagacca tccctacgcc aaagaactgg catcgctggg cctgccgaag cgggctctgt tgtcccagtc ggcggccaac gtagaaatga ccttcggcga cggtcacccg atctaagtga gtaagatagg atccggctgc tacagncccc gaaagnnact cnnnnnnnnn nnnnnnggcn atcgnnancn tttnntntct tctcttatct gcccctcntc tccccaccgc cccantcttg tnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg tctcgcaaca caccatcgcc ggcacggtgc tcaccatgcc ggtgcgcatt cgcaccgcca acctgcattc cgcgatgttc tcggtgcccg ccgacccagc gcagcgcctc atcgactaca gcgggctgcg ggtgtgcgaa tacctgcccg gtaaggcaat cgtgatgcag atgctggtgc gctacgtcga cggggatttg gggcgatacc acgagtacgg caccgcgatc atggtgaacc cgcccggcac ccaacgccgc gggcccagag ccctcacccg agccgccgcg ttcatccatc atctgccggt agatcaggtg ttcacgcttg aggccgggcg caccatctgg ggcttcccga agatcatggc ggacttcaac gtcaccgacg gccggaggtt cggcttcgac gtcagcgccg acggacggtt gatcgccggg atcgagttca gcaccggcct gccggtgccg accctcgggt ggcaaatgtt gaagacctac tcccaccatg acggcgtaac tcgcgagatt ccctgggaaa tgaaagtctc gggcctgcgc gcccggctcg gcggcgcccg actgcggttg ggagaccatc cctacgccaa agaactggca tcgctgggcc tgccgaagcg ggctctgttg tcccagtcgg cggccaacgt agaaatgacc ttcggcgacg gtcacccgat ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat