MytuD.18773.a

Conserved protein

CENTER ID: MytuD.18773.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18773.a.B1.GE37925 full length 1 435
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1381
RefSeq: NP_215748.1
RefSeq: YP_006514607.1
TubercuList: Rv1232c
MTB Network Portal: Rv1232c
UniProt: O86315

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSVNRVYLA RLSRMSVLGP LGESFGRVRD VVISISIVRQ QPRVLGLVVD LATRRKIFIP ILRVAAIEPH AVTLSTGNVS LHRFEQRPGE ALALGQVLDT LVKVNDPALP ELAGVDVVVT DLGVEQTRSR DWMVTRVAVR TQRRLRRRCP VHVVDWHNVA GLTPSALAMP GQDVAQLLDQ FEGWKAVDVA DAIRGLPPKR RHEVFKALHD KRLADVLQEL PELDQAEVLS QLGTERAADV LEEMDPDDAA DLLAVLNPTE AELLLTRMDP GDSGQVRRLL THSPDTAGGL MTSDPVVLTP DTSIAEALAR VRDPDLTPAL ASMVFVARPP TATPTGHYLG CVHLQRLLRD PPAELVGGVV DTDLLTLTPE TPLAAVTRYF AAYNLVCGPV VDDENHLLGA VTVDDLLDHL LPHDWRVDMP ELDPSGAPDR PGGPR
NT Sequence
atgggatcgg tcaacagggt gtacctggcg cggctctcgc ggatgtcggt gctgggaccg ctcggcgaat ccttcgggcg cgttcgcgat gtcgtgatca gcatcagcat tgtccgccaa caaccgcgag tgctcgggct ggtggtcgat ttggcgaccc gccgcaagat attcataccg attctgcggg tcgccgcgat cgagccgcac gcggtgacac tgagcaccgg caacgtgtca ctgcaccgct tcgagcagcg gccaggcgag gctctggcgc tgggtcaagt gctcgacacc ctggtgaagg tcaacgatcc cgcactaccc gagctggccg gcgtcgacgt cgtggtcacc gacctgggtg tcgagcaaac ccgaagtcgc gactggatgg tgactagggt cgccgtccgc acccaacgtc ggctccgacg gcgctgccca gtgcacgtcg tggactggca caacgtggcg gggttgacgc cgtcggcctt ggcgatgccg ggtcaagacg tggcacagct gctcgaccag ttcgagggat ggaaagcggt cgacgtggcc gacgccatcc gcgggctgcc gccgaagcgc cgccacgagg tgttcaaggc gctccacgac aagcgcttgg ccgacgtcct gcaggagctg cccgaactgg atcaggccga ggtgttgtcg caactgggca ccgaacgcgc ggccgacgtg ctggaggaga tggatcccga cgacgccgcc gatctgctcg cggtcctgaa tccgaccgaa gccgagttgc tgctgacacg aatggatccc ggcgactccg ggcaggtgcg aaggctgctg acgcactccc ccgacacggc gggcggattg atgacttccg atccggtggt gttgaccccg gacacctcga ttgcggaggc gttggcgcgg gtccgcgatc ccgatctcac ccccgccctg gcgtcaatgg tgttcgtcgc gcgcccgccc acggccacgc ccactgggca ctacctgggt tgtgtgcatt tgcagcggct gcttcgtgac ccgccggccg agctggtcgg cggagttgtg gacactgacc tgctcacgct cactccggag accccgctgg ccgcggtgac tcgctacttc gccgcctaca acctggtgtg cggaccggtg gttgacgacg agaaccacct gctgggagcg gtgaccgtgg acgacctgct cgaccatcta ttgccgcatg actggcgtgt agatatgccg gagctcgacc cctccggagc accggacaga cccggaggac cacggtga
Details for MytuD.18773.a.B1.GE37925
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MSVLGPLGES FGRVRDVVIS ISIVRQQPRV LGLVVDLATR RKIFIPILRV AAIEPHAVTL STGNVSLHRF XQRPGEALAL GQVLDTLVKV NDPALPELAG VDVVVTDLGV EQTRSRDWMV TRVAVRTQRR LRRRCPVHVV DWHNVAGLTP SALAMPGQDV AQLLDQFEGW KAVDVADAIR GLPPKRRHEV FKALHDKRLA DVLQELPELD QAEVLSQLGT ERAADVLEEM DPDDAADLLA VLNPTEAELL LTRMDPGDSG QVRRLLTHSP DTAGGLMTSD PVVLTPDTSI AEALARVRDP DLTPALASMV FVARPPTATP TGHYLGCVHL QRLLRDPPAE LVGGVVDTDL LTLTPETPLA AVTRYFAAYN LVCGPVVDDE NHLLGAVTVD DLLDHLLPHD WRVDMPELDP SGAP
Validated NT Sequence
ccggtgctcc ggaggggtcg agctccggca tatctacacg ccagtcatgc ggcaatagat ggtcgagcag gtcgtccacg gtcaccgctc ccagcaggtg gttctcgtcg tcaaccaccg gtccgcacac caggttgtag gcggcgaagt agcgagtcac cgcggccagc ggggtctccg gagtgagcgt gagcaggtca gtgtccacaa ctccgccgac cagctcggcc ggcgggtcac gaagcagccg ctgcaaatgc acacaaccca ggtagtgccc agtgggcgtg gccgtgggcg ggcgcgcgac gaacaccatt gacgccaggg cgggggtgag atcgggatcg cggacccgcg ccaacgcctc cgcaatcgag gtgtccgggg tcaacaccac cggatcggaa gtcatcaatc cgcccgccgt gtcgggggag tgcgtcagca gccttcgcac ctgcccggag tcgccgggat ccattcgtgt cagcagcaac tcggcttcgg tcggattcag gaccgcgagc agatcggcgg cgtcgtcggg atccatctcc tccagcacgt cggccgcgcg ttcggtgccc agttgcgaca acacctcggc ctgatccagt tcgggcagct cctgcaggac gtcggccaag cgcttgtcgt ggagcgcctt gaacacctcg tggcggcgct tcggcggcag cccgcggatg gcgtcggcca cgtcgaccgc tttccatccc tcgaactggt cgagcagctg tgccacgtct tgacccggca tcgccaaggc cgacggcgtc aaccccgcca cgttgtgcca gtccacgacg tgcactgggc agcgccgtcg gagccgacgt tgggtgcgga cggcgaccct agtcaccatc cagtcgcgac ttcgggtttg ctcgacaccc aggtcggtga ccacgacgtc gacgccggcc agctcgggta gtgcgggatc gttgaccttc accagggtgt cgagcacttg acccagcgcc agagcctcgc ctggccgctg ctngaagcgg tgcagtgaca cgttgccggt gctcagtgtc accgcgtgcg gctcgatcgc ggcgacccgc agaatcggta tgaatatctt gcggcgggtc gccaaatcga ccaccagccc gagcactcgc ggttgttggc ggacaatgct gatgctgatc acgacatcgc gaacgcgccc gaaggattcg ccgagcggtc ccagcaccga catccgcgag agccgcgcca ggtacaccct gttgaccgat ccc
Expected Protein Sequence
MAHHHHHHMG SVNRVYLARL SRMSVLGPLG ESFGRVRDVV ISISIVRQQP RVLGLVVDLA TRRKIFIPIL RVAAIEPHAV TLSTGNVSLH RFEQRPGEAL ALGQVLDTLV KVNDPALPEL AGVDVVVTDL GVEQTRSRDW MVTRVAVRTQ RRLRRRCPVH VVDWHNVAGL TPSALAMPGQ DVAQLLDQFE GWKAVDVADA IRGLPPKRRH EVFKALHDKR LADVLQELPE LDQAEVLSQL GTERAADVLE EMDPDDAADL LAVLNPTEAE LLLTRMDPGD SGQVRRLLTH SPDTAGGLMT SDPVVLTPDT SIAEALARVR DPDLTPALAS MVFVARPPTA TPTGHYLGCV HLQRLLRDPP AELVGGVVDT DLLTLTPETP LAAVTRYFAA YNLVCGPVVD DENHLLGAVT VDDLLDHLLP HDWRVDMPEL DPSGAPDRPG GPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggat cggtcaacag ggtgtacctg gcgcggctct cgcggatgtc ggtgctggga ccgctcggcg aatccttcgg gcgcgttcgc gatgtcgtga tcagcatcag cattgtccgc caacaaccgc gagtgctcgg gctggtggtc gatttggcga cccgccgcaa gatattcata ccgattctgc gggtcgccgc gatcgagccg cacgcggtga cactgagcac cggcaacgtg tcactgcacc gcttcgagca gcggccaggc gaggctctgg cgctgggtca agtgctcgac accctggtga aggtcaacga tcccgcacta cccgagctgg ccggcgtcga cgtcgtggtc accgacctgg gtgtcgagca aacccgaagt cgcgactgga tggtgactag ggtcgccgtc cgcacccaac gtcggctccg acggcgctgc ccagtgcacg tcgtggactg gcacaacgtg gcggggttga cgccgtcggc cttggcgatg ccgggtcaag acgtggcaca gctgctcgac cagttcgagg gatggaaagc ggtcgacgtg gccgacgcca tccgcgggct gccgccgaag cgccgccacg aggtgttcaa ggcgctccac gacaagcgct tggccgacgt cctgcaggag ctgcccgaac tggatcaggc cgaggtgttg tcgcaactgg gcaccgaacg cgcggccgac gtgctggagg agatggatcc cgacgacgcc gccgatctgc tcgcggtcct gaatccgacc gaagccgagt tgctgctgac acgaatggat cccggcgact ccgggcaggt gcgaaggctg ctgacgcact cccccgacac ggcgggcgga ttgatgactt ccgatccggt ggtgttgacc ccggacacct cgattgcgga ggcgttggcg cgggtccgcg atcccgatct cacccccgcc ctggcgtcaa tggtgttcgt cgcgcgcccg cccacggcca cgcccactgg gcactacctg ggttgtgtgc atttgcagcg gctgcttcgt gacccgccgg ccgagctggt cggcggagtt gtggacactg acctgctcac gctcactccg gagaccccgc tggccgcggt gactcgctac ttcgccgcct acaacctggt gtgcggaccg gtggttgacg acgagaacca cctgctggga gcggtgaccg tggacgacct gctcgaccat ctattgccgc atgactggcg tgtagatatg ccggagctcg acccctccgg agcaccggac agacccggag gaccacggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat