SSGCID: Target Page

MytuD.18769.a

Uncharacterized protein Rv2629/MT2704

CENTER ID:	MytuD.18769.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18769.a.B1.GE37923	full length		1	374

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2939
RefSeq:	NP_217145.1
RefSeq:	YP_006516072.1
TubercuList:	Rv2629
MTB Network Portal:	Rv2629
UniProt:	P9WL63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSERLRWLV AAEGPFASVY FDDSHDTLDA VERREATWRD VRKHLESRDA KQELIDSLEE AVRDSRPAVG QRGRALIATG EQVLVNEHLI GPPPATVIRL SDYPYVVPLI DLEMRRPTYV FAAVDHTGAD VKLYQGATIS STKIDGVGYP VHKPVTAGWN GYGDFQHTTE EAIRMNCRAV ADHLTRLVDA ADPEVVFVSG EVRSRTDLLS TLPQRVAVRV SQLHAGPRKS ALDEEEIWDL TSAEFTRRRY AEITNVAQQF EAEIGRGSGL AAQGLAEVCA ALRDGDVDTL IVGELGEATV VTGKARTTVA RDADMLSELG EPVDRVARAD EALPFAAIAV GAALVRDDNR IAPLDGVGAL LRYAATNRLG SHRS

NT Sequence

atgcgatcag aacgtctccg gtggctggta gccgcagaag gtccgttcgc ctcggtgtat ttcgacgact cgcacgacac tcttgatgcc gtcgagcgcc gggaagcgac gtggcgcgat gtccggaagc atctcgaaag ccgcgacgcg aagcaggagc tcatcgacag cctcgaagag gcggtgcggg attctcgacc ggccgtcggc cagcgtggcc gcgcgctgat cgcgaccggc gagcaagtac tggtcaacga gcatctgatc ggcccaccac cggctacggt gattcggctg tcggattatc cgtacgtcgt gccattgata gaccttgaga tgcggcgacc gacgtatgta tttgccgcgg ttgatcacac cggcgccgac gtcaagctgt atcagggggc caccatcagt tccacgaaaa tcgatggggt cggctacccg gtgcacaagc cggtcaccgc cggctggaac ggctacggcg acttccagca caccaccgaa gaagccatcc gaatgaactg ccgcgcggtc gccgaccatc tcacccgact ggtagacgct gccgaccccg aggtggtgtt cgtgtccggc gaggtgcggt cacgcacaga cctgctttcc acattgccgc agcgggtggc ggtccgggtg tcgcagctgc atgccggacc gcgcaaaagc gccttagacg aggaagagat ctgggacctg acatccgcgg agttcacccg gcggcggtac gccgaaatca ccaatgtcgc acaacaattt gaggcggaga tcggacgcgg atcggggctg gcggcccaag ggttggcgga ggtgtgtgcg gctctgcgtg acggcgacgt cgacacgctg atcgtcggag agctaggcga ggccaccgtg gtcaccggta aagcgcgtac tacggtcgcg cgggatgccg acatgttgtc cgaactcggc gaaccggtag atcgcgtggc aagggccgat gaggcgttgc cattcgccgc gatcgcggta ggtgccgcat tggtccgtga cgacaaccgg atcgcgccac tagatggggt gggcgcattg ctgcgttatg ccgccaccaa ccgactcggc agccatagat cctag

Details for MytuD.18769.a.B1.GE37923

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	42kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	32
PERCENT COVERAGE:	47

Validated AA Sequence

MVGDRAAVHS DGFFGGVLEV AVXVPAGGDR LVHRVADPID FRGTDGGPLI QLDVGAGVIN RGKYIRRSPH LKVYQWHDVR IIRQPNHRSR WWADQMLVDQ YLLAGRDQRA ATLADGRSRI PHXLFEAVDE LLLRVAAXEM LPDIAPRRFP ALDGIKSVVR VVEIHRGERT FCGYQPPETF

Validated NT Sequence

aatgcgccca ccccatctag tggcgcgatc cggttgtcgt cacggaccaa tgcggcacct accgcgatcg cggcgaatgg caacgcctca tcggcccttg ccacgcgatc taccggttcg ccgagttcgg acaacatgtc ggcatcccgc gcgaccgtag tacgcgcttt accggtgacc acggtggcct cgcctagctc tccgacgatc agcgtgtcga cgtcgccgtc acgcagagcc gcacacacct ccgccaaccc ttgggccgcc agccccgatc cgcgtccgat ctccgcctca aattgttgtg cgacattggt gatttcggcg taccgccgcc gggtgaactc cgcggatgtc aggtcccaga tctcttcctc gtctaaggcg cttttgcgcg gtccgggcat gcagctgcga cacccggacc gccacccgct gcggcaatgt ggaaagcagg tctgtgcgtg accgcacctc gccggacacg aacaccacct cggggtcggc agcgtctacc agtcgggtga gatggtcggc gaccgcgcgg cagttcattc ggatggcttc ttcggtggtg tgctggaagt cgccgtannn gttccagccg gcggtgaccg gcttgtgcac cgggtagccg accccatcga ttttcgtgga actgatggtg gccccctgat acagcttgac gtcggcgccg gtgtgatcaa ccgcggcaaa tacatacgtc ggtcgccgca tctcaaggtc tatcaatggc acgacgtacg gataatccga cagccgaatc accgtagccg gtggtgggcc gatcagatgc tcgttgacca gtacttgctc gccggtcgcg atcagcgcgc ggccacgctg gccgacggcc ggtcgagaat cccgcacngc ctcttcgagg ctgtcgatga gctcctgctt cgcgtcgcgg ctttngagat gcttccggac atcgcgccac gtcgcttccc ggcgctcgac ggcatcaaga gtgtcgtgcg agtcgtcgaa atacaccgag gcgaacggac cttctgcggc taccagccac cggagacgtt ctgatcgc

Expected Protein Sequence

MAHHHHHHMR SERLRWLVAA EGPFASVYFD DSHDTLDAVE RREATWRDVR KHLESRDAKQ ELIDSLEEAV RDSRPAVGQR GRALIATGEQ VLVNEHLIGP PPATVIRLSD YPYVVPLIDL EMRRPTYVFA AVDHTGADVK LYQGATISST KIDGVGYPVH KPVTAGWNGY GDFQHTTEEA IRMNCRAVAD HLTRLVDAAD PEVVFVSGEV RSRTDLLSTL PQRVAVRVSQ LHAGPRKSAL DEEEIWDLTS AEFTRRRYAE ITNVAQQFEA EIGRGSGLAA QGLAEVCAAL RDGDVDTLIV GELGEATVVT GKARTTVARD ADMLSELGEP VDRVARADEA LPFAAIAVGA ALVRDDNRIA PLDGVGALLR YAATNRLGSH RS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgat cagaacgtct ccggtggctg gtagccgcag aaggtccgtt cgcctcggtg tatttcgacg actcgcacga cactcttgat gccgtcgagc gccgggaagc gacgtggcgc gatgtccgga agcatctcga aagccgcgac gcgaagcagg agctcatcga cagcctcgaa gaggcggtgc gggattctcg accggccgtc ggccagcgtg gccgcgcgct gatcgcgacc ggcgagcaag tactggtcaa cgagcatctg atcggcccac caccggctac ggtgattcgg ctgtcggatt atccgtacgt cgtgccattg atagaccttg agatgcggcg accgacgtat gtatttgccg cggttgatca caccggcgcc gacgtcaagc tgtatcaggg ggccaccatc agttccacga aaatcgatgg ggtcggctac ccggtgcaca agccggtcac cgccggctgg aacggctacg gcgacttcca gcacaccacc gaagaagcca tccgaatgaa ctgccgcgcg gtcgccgacc atctcacccg actggtagac gctgccgacc ccgaggtggt gttcgtgtcc ggcgaggtgc ggtcacgcac agacctgctt tccacattgc cgcagcgggt ggcggtccgg gtgtcgcagc tgcatgccgg accgcgcaaa agcgccttag acgaggaaga gatctgggac ctgacatccg cggagttcac ccggcggcgg tacgccgaaa tcaccaatgt cgcacaacaa tttgaggcgg agatcggacg cggatcgggg ctggcggccc aagggttggc ggaggtgtgt gcggctctgc gtgacggcga cgtcgacacg ctgatcgtcg gagagctagg cgaggccacc gtggtcaccg gtaaagcgcg tactacggtc gcgcgggatg ccgacatgtt gtccgaactc ggcgaaccgg tagatcgcgt ggcaagggcc gatgaggcgt tgccattcgc cgcgatcgcg gtaggtgccg cattggtccg tgacgacaac cggatcgcgc cactagatgg ggtgggcgca ttgctgcgtt atgccgccac caaccgactc ggcagccata gatcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat