MytuD.18768.a

Conserved protein

CENTER ID: MytuD.18768.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18768.a.B1.GE37922 full length 1 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3640
RefSeq: NP_217773.1
RefSeq: YP_006516732.1
TubercuList: Rv3256c
MTB Network Portal: Rv3256c
UniProt: O05899

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVARAIDLE DTEGLIAADR GALLRAASMA GAQVRAIAAA ADEGELDLLR GSDRPRSVIW VTGRGTAETA GTILASTLGA GAAEPIVLAS AAPPWVGPLD VLIVAGDDPG DPALVGAAAI GVRRGARVVV VAPYEGPLRD STAGRVAVLE PRLRVPDEFG LSRYLAAGLA ALQTVDPKLR IDLASLADEL DAEALRNSAG REVFTNPAKA LAARVSGCQL ALAGDNAATL ALARHGSSVM LRIANQVVAA TRLSDAVVAL RAGTPPDALF HDEEIDGPAP QRLRVLALAL AGERTVVAAR VAGLDDAYLV AAEDVPELLD APVGSGGAVL AVRLEMAAVY LRLVRG
NT Sequence
gtgaacgtcg cccgggcgat cgatctcgag gacaccgaag gcctgatcgc tgccgaccgg ggtgccctgc tgcgggccgc atcgatggcc ggcgcacagg tgcgtgccat cgctgccgcg gccgacgagg gcgaactgga cttgctgcgc ggtagtgacc gtccccgctc ggtgatctgg gtgaccggcc gggggaccgc tgagaccgcc gggaccatcc tggcctcgac gctaggtgcc ggggccgccg agccgatcgt gctcgccagt gcggcgccgc cctgggtcgg gccgcttgac gtgctgatcg ttgccggcga cgaccccggc gacccggcgc tggtcggggc cgccgcgatc ggggtgcgca ggggcgcgcg ggttgtcgtg gtggcaccgt atgagggtcc gctgcgggac agcacggccg gtcgcgtcgc ggtgctggaa ccgcggctgc gggttcctga cgagttcggg ttgtcccggt acctggccgc gggtctggcg gccttgcaaa ccgtggatcc caagctgcgc atcgatctgg cgtcgctggc agacgagctg gatgctgagg cgctccgcaa cagcgccggc cgagaggtgt tcaccaaccc ggccaaggca cttgccgctc gcgtttccgg ttgccagctg gccctggctg gagacaatgc cgcgacgctg gcgctggccc ggcatgggag ttcggtcatg ctgcggatcg caaaccaggt tgtggccgct accaggcttt cggatgcggt tgtggcgcta cgtgccggga cgccgccgga tgcgctgttc catgacgagg aaatcgatgg gccggcaccg cagcggctgc gggtgttggc gctggcgctg gccggcgagc ggacggtggt ggccgctcgg gttgccgggc tcgatgacgc ctatctggtc gcggctgagg atgtgccgga gctgctcgac gcccccgtgg gatccggggg ggcggtattg gccgttcggc tggagatggc cgccgtgtat ctgcgactgg tgcgaggata g
Details for MytuD.18768.a.B1.GE37922
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 36kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MVNVARAIDL EDTEGLIAAD RGALLRAASM AGAQVRAIAA AADEGELDLL RGSDRPRSVI WVTGRGTAET AGTILASTLG AGAAEPIVLA SAAPPWVGPL DVLIVAGDDP GDPALVGAAA IGVRRGARVV VVAPYEGPLR DSTAGRVAVL EPRLRVPDEF GLSRYLAAGL AALQTVDPKL RIDLASLADE LDAEALRNSA GREVFTNPAK ALAARVSGCQ LALAGDNAXT LALARHGSSV MLRIANQVVA ATRLSDAVVA LRAGTPPDAL FHDEEIDGPA PQRLRVLALA LAGERTVVAA RVAGLDDAYL VAAEDVPELL DAPVGSGGAV LAVRLEMAA
Validated NT Sequence
acggcggcca tctccagccg aacggccaat accgcccccc cggatcccac gggggcgtcg agcagctccg gcacatcctc agccgcgacc agataggcgt catcgagccc ggcaacccga gcggccacca ccgtccgctc gccggccagc gccagcgcca acacccgcag ccgctgcggt gccggcccat cgatttcctc gtcatggaac agcgcatccg gcggcgtccc ggcacgtagc gccacaaccg catccgaaag cctggtagcg gccacaacct ggtttgcgat ccgcagcatg accgaactcc catgccgggc cagcgccagc gtcncggcat tgtctccagc cagggccagc tggcaaccgg aaacgcgagc ggcaagtgcc ttggccgggt tggtgaacac ctctcggccg gcgctgttgc ggagcgcctc agcatccagc tcgtctgcca gcgacgccag atcgatgcgc agcttgggat ccacggtttg caaggccgcc agacccgcgg ccaggtaccg ggacaacccg aactcgtcag gaacccgcag ccgcggttcc agcaccgcga cgcgaccggc cgtgctgtcc cgcagcggac cctcatacgg tgccaccacg acaacccgcg cgcccctgcg caccccgatc gcggcggccc cgaccagcgc cgggtcgccg gggtcgtcgc cggcaacgat cagcacgtca agcggcccga cccagggcgg cgccgcactg gcgagcacga tcggctcggc ggccccggca cctagcgtcg aggccaggat ggtcccggcg gtctcagcgg tcccccggcc ggtcacccag atcaccgagc ggggacggtc actaccgcgc agcaagtcca gttcgccctc gtcggccgcg gcagcgatgg cacgcacctg tgcgccggcc atcgatgcgg cccgcagcag ggcaccccgg tcggcagcga tcaggccttc ggtgtcctcg agatcgatcg cccgggcgac gttcaccat
Expected Protein Sequence
MAHHHHHHMN VARAIDLEDT EGLIAADRGA LLRAASMAGA QVRAIAAAAD EGELDLLRGS DRPRSVIWVT GRGTAETAGT ILASTLGAGA AEPIVLASAA PPWVGPLDVL IVAGDDPGDP ALVGAAAIGV RRGARVVVVA PYEGPLRDST AGRVAVLEPR LRVPDEFGLS RYLAAGLAAL QTVDPKLRID LASLADELDA EALRNSAGRE VFTNPAKALA ARVSGCQLAL AGDNAATLAL ARHGSSVMLR IANQVVAATR LSDAVVALRA GTPPDALFHD EEIDGPAPQR LRVLALALAG ERTVVAARVA GLDDAYLVAA EDVPELLDAP VGSGGAVLAV RLEMAAVYLR LVRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaacg tcgcccgggc gatcgatctc gaggacaccg aaggcctgat cgctgccgac cggggtgccc tgctgcgggc cgcatcgatg gccggcgcac aggtgcgtgc catcgctgcc gcggccgacg agggcgaact ggacttgctg cgcggtagtg accgtccccg ctcggtgatc tgggtgaccg gccgggggac cgctgagacc gccgggacca tcctggcctc gacgctaggt gccggggccg ccgagccgat cgtgctcgcc agtgcggcgc cgccctgggt cgggccgctt gacgtgctga tcgttgccgg cgacgacccc ggcgacccgg cgctggtcgg ggccgccgcg atcggggtgc gcaggggcgc gcgggttgtc gtggtggcac cgtatgaggg tccgctgcgg gacagcacgg ccggtcgcgt cgcggtgctg gaaccgcggc tgcgggttcc tgacgagttc gggttgtccc ggtacctggc cgcgggtctg gcggccttgc aaaccgtgga tcccaagctg cgcatcgatc tggcgtcgct ggcagacgag ctggatgctg aggcgctccg caacagcgcc ggccgagagg tgttcaccaa cccggccaag gcacttgccg ctcgcgtttc cggttgccag ctggccctgg ctggagacaa tgccgcgacg ctggcgctgg cccggcatgg gagttcggtc atgctgcgga tcgcaaacca ggttgtggcc gctaccaggc tttcggatgc ggttgtggcg ctacgtgccg ggacgccgcc ggatgcgctg ttccatgacg aggaaatcga tgggccggca ccgcagcggc tgcgggtgtt ggcgctggcg ctggccggcg agcggacggt ggtggccgct cgggttgccg ggctcgatga cgcctatctg gtcgcggctg aggatgtgcc ggagctgctc gacgcccccg tgggatccgg gggggcggta ttggccgttc ggctggagat ggccgccgtg tatctgcgac tggtgcgagg atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat