SSGCID: Target Page

MytuD.18767.a

Uncharacterized protein Rv1480/MT1527

CENTER ID:	MytuD.18767.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18767.a.B1.GE37921	full length		1	317

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.1652
RefSeq:	NP_215996.1
RefSeq:	YP_006514864.1
TubercuList:	Rv1480
MTB Network Portal:	Rv1480
UniProt:	P9WLX5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTESKAPAVV HPPSMLRGDI DDPKLAAALR TLELTVKQKL DGVLHGDHLG LIPGPGSEPG ESRLYQPGDD VRRMDWAVTA RTTHPHVRQM IADRELETWL VVDMSASLDF GTACCEKRDL AVAAAAAITF LNSGGGNRLG ALIANGAAMT RVPARTGRQH QHTMLRTIAT MPQAPAGVRG DLAVAIDALR RPERRRGMAV IISDFLGPIN WMRPLRAIAA RHEVLAIEVL DPRDVELPDV GDVVLQDAES GVVREFSIDP ALRDDFARAA AAHRADVART IRGCGAPLLS LRTDRDWLAD IVRFVASRRR GALAGHQ

NT Sequence

GTGACCGAAT CCAAAGCGCC GGCGGTGGTG CATCCGCCGT CGATGCTGCG CGGGGACATC GACGACCCGA AGCTGGCGGC GGCGCTGCGC ACCCTCGAGT TGACCGTCAA GCAGAAGCTC GACGGTGTCT TGCACGGCGA TCACCTCGGC CTGATACCTG GGCCGGGTTC GGAGCCAGGG GAGTCGCGCC TCTACCAGCC CGGTGACGAT GTCCGCCGGA TGGACTGGGC GGTCACCGCT CGCACCACTC ACCCGCATGT CCGGCAGATG ATCGCCGACC GGGAACTGGA AACCTGGCTG GTGGTCGACA TGTCGGCCAG CCTGGATTTT GGCACCGCCT GCTGCGAGAA ACGTGACCTC GCGGTGGCGG CGGCGGCTGC CATCACCTTC CTCAACAGCG GCGGCGGCAA CCGGCTCGGT GCGCTGATCG CCAACGGCGC CGCGATGACT CGGGTGCCGG CTCGCACCGG GCGCCAACAT CAGCACACGA TGTTGCGCAC CATTGCGACC ATGCCGCAGG CCCCTGCGGG GGTCCGCGGC GACCTGGCGG TTGCCATCGA TGCGCTGCGC CGGCCCGAAC GTCGTCGCGG GATGGCGGTG ATCATCAGCG ATTTTCTGGG CCCGATCAAC TGGATGCGTC CGCTGCGGGC GATCGCAGCC CGCCATGAGG TGCTGGCCAT CGAAGTGCTC GATCCGCGCG ATGTCGAATT GCCGGACGTG GGTGATGTGG TGCTGCAGGA CGCCGAATCC GGGGTTGTGC GCGAGTTCAG CATCGACCCT GCGCTGCGCG ACGACTTCGC TAGGGCAGCT GCGGCGCACC GGGCCGACGT GGCGCGCACC ATCCGCGGTT GCGGGGCACC CTTGCTATCG CTTCGCACCG ACCGCGACTG GCTTGCCGAT ATCGTACGAT TCGTCGCCTC TCGCCGGCGT GGGGCATTGG CGGGACACCA GTGA

Details for MytuD.18767.a.B1.GE37921

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/9/2014
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MVTESKAPAV VHPPSMLRGD IDDPKLAAAL RTLELTVKQK LDGVLHGDHL GLIPGPGSEP GESRLYQPGD DVRRMDWAVT ARTTHPHVRQ MIADRELETW LVVDMSASLD FGTACCEKRD LAVAAAAAIT FLNSGGGNRL GALIANGAAM TRVPARTGRQ HQHTMLRTIA TMPQAPAGVR GDLAVAIDAL RRPERRRGMA VIISDFLGPI NWMRPLRAIA ARHEVLAIEV LDPRDVELPD VGDVVLQDAE SGVVREFSID PALRDDFARA AAAHRADVAR TIRGCGAPLL SLRTDRDWLA DIVRFVASRR RGALAGHQ

Validated NT Sequence

atcttactca cttactggtg tcccgccaat gccccacgcc ggcgagaggc gacgaatcgt acgatatcgg caagccagtc gcggtcggtg cgaagcgata gcaagggtgc cccgcaaccg cggatggtgc gcgccacgtc ggcccggtgc gccgcagctg ccctagcgaa gtcgtcgcgc agcgcagggt cgatgctgaa ctcgcgcaca accccggatt cggcgtcctg cagcaccaca tcacccacgt ccggcaattc gacatcgcgc ggatcgagca cttcgatggc cagcacctca tggcgggctg cgatcgcccg cagcggacgc atccagttga tcgggcccag aaaatcgctg atgatcaccg ccatcccgcg acgacgttcg ggccggcgca gcgcatcgat ggcaaccgcc aggtcgccgc ggacccccgc aggggcctgc ggcatggtcg caatggtgcg caacatcgtg tgctgatgtt ggcgcccggt gcgagccggc acccgagtca tcgcggcgcc gttggcgatc agcgcaccga gccggttgcc gccgccgctg ttgaggaagg tgatggcagc cgccgccgcc accgcgaggt cacgtttctc gcagcaggcg gtgccaaaat ccaggctggc cgacatgtcg accaccagcc aggtttccag ttcccggtcg gcgatcatct gccggacatg cgggtgagtg gtgcgagcgg tgaccgccca gtccatccgg cggacatcgt caccgggctg gtagaggcgc gactcccctg gctccgaacc cggcccaggt atcaggccga ggtgatcgcc gtgcaagaca ccgtcgagct tctgcttgac ggtcaactcg agggtgcgca gcgccgccgc cagcttcggg tcgtcgatgt ccccgcgcag catcgacggc ggatgcacca ccgccggcgc tttggattcg gtcaccatat ggtggtggtg gtggtgagcc a

Expected Protein Sequence

MAHHHHHHMT ESKAPAVVHP PSMLRGDIDD PKLAAALRTL ELTVKQKLDG VLHGDHLGLI PGPGSEPGES RLYQPGDDVR RMDWAVTART THPHVRQMIA DRELETWLVV DMSASLDFGT ACCEKRDLAV AAAAAITFLN SGGGNRLGAL IANGAAMTRV PARTGRQHQH TMLRTIATMP QAPAGVRGDL AVAIDALRRP ERRRGMAVII SDFLGPINWM RPLRAIAARH EVLAIEVLDP RDVELPDVGD VVLQDAESGV VREFSIDPAL RDDFARAAAA HRADVARTIR GCGAPLLSLR TDRDWLADIV RFVASRRRGA LAGHQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg aatccaaagc gccggcggtg gtgcatccgc cgtcgatgct gcgcggggac atcgacgacc cgaagctggc ggcggcgctg cgcaccctcg agttgaccgt caagcagaag ctcgacggtg tcttgcacgg cgatcacctc ggcctgatac ctgggccggg ttcggagcca ggggagtcgc gcctctacca gcccggtgac gatgtccgcc ggatggactg ggcggtcacc gctcgcacca ctcacccgca tgtccggcag atgatcgccg accgggaact ggaaacctgg ctggtggtcg acatgtcggc cagcctggat tttggcaccg cctgctgcga gaaacgtgac ctcgcggtgg cggcggcggc tgccatcacc ttcctcaaca gcggcggcgg caaccggctc ggtgcgctga tcgccaacgg cgccgcgatg actcgggtgc cggctcgcac cgggcgccaa catcagcaca cgatgttgcg caccattgcg accatgccgc aggcccctgc gggggtccgc ggcgacctgg cggttgccat cgatgcgctg cgccggcccg aacgtcgtcg cgggatggcg gtgatcatca gcgattttct gggcccgatc aactggatgc gtccgctgcg ggcgatcgca gcccgccatg aggtgctggc catcgaagtg ctcgatccgc gcgatgtcga attgccggac gtgggtgatg tggtgctgca ggacgccgaa tccggggttg tgcgcgagtt cagcatcgac cctgcgctgc gcgacgactt cgctagggca gctgcggcgc accgggccga cgtggcgcgc accatccgcg gttgcggggc acccttgcta tcgcttcgca ccgaccgcga ctggcttgcc gatatcgtac gattcgtcgc ctctcgccgg cgtggggcat tggcgggaca ccagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat