SSGCID: Target Page

MytuD.18765.a

Conserved protein

CENTER ID:	MytuD.18765.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18765.a.B2.GE37919	soluble domain 1		1	187

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2208
RefSeq:	NP_216494.1
RefSeq:	YP_006515384.1
TubercuList:	Rv1978
MTB Network Portal:	Rv1978
UniProt:	O53979

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGEANIREQA IATMPRGGPD ASWLDRRFQT DALEYLDRDD VPDEVKQKII GVLDRVGTLT NLHEKYARIA LKLVSDIPNP RILELGAGHG KLSAKILELH PTATVTISDL DPTSVANIAA GELGTHPRAR TQVIDATAID GHDHSYDLAV FALAFHHLPP TVACKAIAEA TRVGKRFLII DLKRQKPLSF TLSSVLLLPL HLLLLPWSSM RSSMHDGFIS ALRAYSPSAL QTLARAADPG MQVEILPAPT RLFPPSLAVV FSRSSSAPTE SSECSADRQP GE

NT Sequence

atgggtgagg cgaacatccg cgagcaggcg atcgccacga tgccacgggg tggccccgac gcgtcttggc tggatcgtcg attccagacc gacgcactgg agtacctcga ccgcgacgat gtgcccgatg aggtcaaaca gaagatcatc ggggtgctcg accgggtggg caccctgacc aacctgcacg agaagtacgc ccggatagcc ctgaaacttg tttctgacat tcccaacccg cgaatcctgg aacttggtgc gggccatggc aagctctcag cgaaaatcct cgagctacac ccgacagcga cggtgacgat cagcgatcta gatcccacct cggtggccaa catcgccgcg ggagagctgg gaacacatcc gcgagcacgc acccaagtga tcgacgccac cgcaatcgac ggccacgacc acagctatga cctggcggtc ttcgcgctgg catttcacca cctgccgcct acggtcgcct gcaaagcgat cgccgaggcc acccgggtgg ggaagcgctt tctgatcatc gacctcaaac ggcagaaacc gctgtcgttc acgctctctt cggtgctgct actgccgctc cacctactgc tgctgccatg gtcgtcgatg cgctcgagca tgcacgacgg ctttatcagc gcactacgtg cctacagtcc ctcggcgttg cagacgcttg cccgcgccgc cgatccggga atgcaggttg aaatcttgcc cgcaccgacc aggctattcc cgccatcgct cgccgttgtg ttctcccgtt cgagctcagc gccaacggaa tctagcgagt gctcggccga tcgccaaccc ggcgaatga

Details for MytuD.18765.a.B2.GE37919

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	21kDa
OBSERVED MW:	21kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG EANIREQAIA TMPRGGPDAS WLDRRFQTDA LEYLDRDDVP DEVKQKIIGV LDRVGTLTNL HEKYARIALK LVSDIPNPRI LELGAGHGKL SAKILELHPT ATVTISDLDP TSVANIAAGE LGTHPRARTQ VIDATAIDGH DHSYDLAVFA LAFHHLPPTV ACKAIAEATR VGKRFLIIDL KRQKP

Validated NT Sequence

atcttactca cttacggttt ctgccgtttg aggtcgatga tcagaaagcg cttccccacc cgggtggcct cggcgatcgc tttgcaggcg accgtaggcg gcaggtggtg aaatgccagc gcgaagaccg ccaggtcata gctgtggtcg tggccgtcga ttgcggtggc gtcgatcact tgggtgcgtg ctcgcggatg tgttcccagc tctcccgcgg cgatgttggc caccgaggtg ggatctagat cgctgatcgt caccgtcgct gtcgggtgta gctcgaggat tttcgctgag agcttgccat ggcccgcacc aagttccagg attcgcgggt tgggaatgtc agaaacaagt ttcagggcta tccgggcgta cttctcgtgc aggttggtca gggtgcccac ccggtcgagc accccgatga tcttctgttt gacctcatcg ggcacatcgt cgcggtcgag gtactccagt gcgtcggtct ggaatcgacg atccagccaa gacgcgtcgg ggccaccccg tggcatcgtg gcgatcgcct gctcgcggat gttcgcctca cccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtg aggcgaacat ccgcgagcag gcgatcgcca cgatgccacg gggtggcccc gacgcgtctt ggctggatcg tcgattccag accgacgcac tggagtacct cgaccgcgac gatgtgcccg atgaggtcaa acagaagatc atcggggtgc tcgaccgggt gggcaccctg accaacctgc acgagaagta cgcccggata gccctgaaac ttgtttctga cattcccaac ccgcgaatcc tggaacttgg tgcgggccat ggcaagctct cagcgaaaat cctcgagcta cacccgacag cgacggtgac gatcagcgat ctagatccca cctcggtggc caacatcgcc gcgggagagc tgggaacaca tccgcgagca cgcacccaag tgatcgacgc caccgcaatc gacggccacg accacagcta tgacctggcg gtcttcgcgc tggcatttca ccacctgccg cctacggtcg cctgcaaagc gatcgccgag gccacccggg tggggaagcg ctttctgatc atcgacctca aacggcagaa accgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat