SSGCID: Target Page

MytuD.18764.a

Conserved protein

CENTER ID:	MytuD.18764.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18764.a.B1.GE37918	full length		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.1180
RefSeq:	NP_215572.1
RefSeq:	YP_006514422.1
TubercuList:	Rv1056
MTB Network Portal:	Rv1056
UniProt:	O53404

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVDYPQMAA TRGRIEPAPR RVRGYLGHVL VFDTSAARYV WEVPYYPQYY IPLADVRMEF LRDENHPQRV QLGPSRLHSL VSAGQTHRSA ARVFDVDGDS PVAGTVRFNW DPLRWFEEDE PIYGHPRNPY QRADALRSHR HVRVELDGIV LADTRSPVLL FETGIPTRYY IDPADIAFEH LEPTSTQTLC PYKGTTSGYW SVRVGDAVHR DLAWTYHYPL PAVAPIAGLV AFYNEKVDLT VDGVALPRPH TQFS

NT Sequence

atgagcgtgg attaccccca aatggctgct acccggggaa gaatagaacc ggccccgcgg cgagttcgcg gctatctcgg acatgtgctc gtcttcgaca ccagtgcggc gcgctatgtc tgggaggttc cctactaccc gcagtactac atcccgctgg cggatgtccg catggagttc ctgcgcgacg agaaccaccc gcagcgagtg cagctgggtc cgtcgcggct gcactccttg gtaagcgccg gtcagaccca ccgatcggcg gcgcgggtat tcgatgtcga cggcgacagc ccggtggcgg gcaccgtgcg tttcaactgg gatccgctgc ggtggttcga ggaggacgag ccgatctacg gccatccgcg caatccctat cagcgggccg atgcgctgcg ctcgcaccga cacgtccgtg tcgagctgga cggcattgtg ctcgctgaca cccgatcgcc cgttctgcta ttcgaaaccg ggatacccac aaggtattac atcgatccgg ccgacatcgc tttcgagcat ctggagccca cctcgacgca gacgttgtgt ccgtacaagg ggacgacgtc gggctattgg tctgtgcgcg tcggcgacgc cgtgcaccgc gacctggcct ggacgtatca ctatccactg cccgccgttg ccccgatcgc cggcctggtg gcgttttaca acgagaaggt cgacctcacc gtcgacggcg tcgccctgcc gcggccgcac actcagttca gctag

Details for MytuD.18764.a.B1.GE37918

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS VDYPQMAATR GRIEPAPRRV RGYLGHVLVF DTSAARYVWE VPYYPQYYIP LADVRMEFLR DENHPQRVQL GPSRLHSLVS AGQTHRSAAR VFDVDGDSPV AGTVRFNWDP LRWFEEDEPI YGHPRNPYQR ADALRSHRHV RVELDGIVLA DTRSPVLLFE TGIPTRYYID PADIAFEHLE PTSTQTLCPY KGTTSGYWSV RVGDAVHRDL AWTYHYPLPA VAPIAGLVAF YNEKVDLTVD GVALPRPHTQ FS

Validated NT Sequence

atcttactca cttagctgaa ctgagtgtgc ggccgcggca gggcgacgcc gtcgacggtg aggtcgacct tctcgttgta aaacgccacc aggccggcga tcggggcaac ggcgggcagt ggatagtgat acgtccaggc caggtcgcgg tgcacggcgt cgccgacgcg cacagaccaa tagcccgacg tcgtcccctt gtacggacac aacgtctgcg tcgaggtggg ctccagatgc tcgaaagcga tgtcggccgg atcgatgtaa taccttgtgg gtatcccagt ttcgaatagc agaacgggcg atcgggtgtc agcgagcaca atgccgtcca gctcgacacg gacgtgtcgg tgcgagcgca gcgcatcggc ccgctgatag ggattgcgcg gatggccgta gatcggctcg tcctcctcga accaccgcag cggatcccag ttgaaacgca cggtgcccgc caccgggctg tcgccgtcga catcgaatac ccgcgccgcc gatcggtggg tctgaccggc gcttaccaag gagtgcagcc gcgacggacc cagctgcact cgctgcgggt ggttctcgtc gcgcaggaac tccatgcgga catccgccag cgggatgtag tactgcgggt agtagggaac ctcccagaca tagcgcgccg cactggtgtc gaagacgagc acatgtccga gatagccgcg aactcgccgc ggggccggtt ctattcttcc ccgggtagca gccatttggg ggtaatccac gctcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tggattaccc ccaaatggct gctacccggg gaagaataga accggccccg cggcgagttc gcggctatct cggacatgtg ctcgtcttcg acaccagtgc ggcgcgctat gtctgggagg ttccctacta cccgcagtac tacatcccgc tggcggatgt ccgcatggag ttcctgcgcg acgagaacca cccgcagcga gtgcagctgg gtccgtcgcg gctgcactcc ttggtaagcg ccggtcagac ccaccgatcg gcggcgcggg tattcgatgt cgacggcgac agcccggtgg cgggcaccgt gcgtttcaac tgggatccgc tgcggtggtt cgaggaggac gagccgatct acggccatcc gcgcaatccc tatcagcggg ccgatgcgct gcgctcgcac cgacacgtcc gtgtcgagct ggacggcatt gtgctcgctg acacccgatc gcccgttctg ctattcgaaa ccgggatacc cacaaggtat tacatcgatc cggccgacat cgctttcgag catctggagc ccacctcgac gcagacgttg tgtccgtaca aggggacgac gtcgggctat tggtctgtgc gcgtcggcga cgccgtgcac cgcgacctgg cctggacgta tcactatcca ctgcccgccg ttgccccgat cgccggcctg gtggcgtttt acaacgagaa ggtcgacctc accgtcgacg gcgtcgccct gccgcggccg cacactcagt tcagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat